Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,454,724 G→T 100% G472C (GGT→TGT)  paaH → 3‑hydroxyadipyl‑CoA dehydrogenase, NAD+‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,454,7240GT99.5% 728.7 / ‑4.3 240G472C (GGT→TGT) paaH3‑hydroxyadipyl‑CoA dehydrogenase, NAD+‑dependent
Reads supporting (aligned to +/- strand):  ref base G (2/0);  new base T (115/123);  total (117/123)
Fisher's exact test for biased strand distribution p-value = 2.37e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 240 total aligned reads displayed.

AATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGG  >  CP009273/1454580‑1454867
                                                                                                                                                |                                                                                                                                               
 aTTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTa                                                                                                                                             >  1:1024786/1‑148 (MQ=255)
   tATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATg                                                                                                                                           >  1:3119427/1‑148 (MQ=255)
      ccATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGt                                                                                                                                        >  1:963051/1‑148 (MQ=255)
      ccATATCGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGt                                                                                                                                        <  1:12088/148‑1 (MQ=255)
       cATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTc                                                                                                                                       <  2:878568/148‑1 (MQ=255)
         tatGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCAt                                                                                                                                     >  2:1966992/1‑148 (MQ=255)
             gCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAgg                                                                                                                                 >  2:2142783/1‑148 (MQ=255)
              cccACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGc                                                                                                                                <  1:1932020/148‑1 (MQ=255)
              cccACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGc                                                                                                                                <  2:3351104/148‑1 (MQ=255)
               ccACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGcc                                                                                                                               >  1:2713172/1‑148 (MQ=255)
               ccACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGcc                                                                                                                               >  1:2712305/1‑148 (MQ=255)
               ccACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGcc                                                                                                                               <  1:689581/148‑1 (MQ=255)
                cACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCt                                                                                                                              <  2:1122679/148‑1 (MQ=255)
                 aCTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTg                                                                                                                             >  2:2247028/1‑148 (MQ=255)
                  cTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGATTCATAATGCCTgg                                                                                                                            >  1:3226796/1‑148 (MQ=255)
                    tGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCa                                                                                                                          >  1:1337813/1‑148 (MQ=255)
                     gCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCaa                                                                                                                         <  2:449929/148‑1 (MQ=255)
                      ccTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCaaa                                                                                                                        >  2:2848499/1‑148 (MQ=255)
                      ccTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCaaa                                                                                                                        <  2:2032228/148‑1 (MQ=255)
                      ccTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCaaa                                                                                                                        <  1:243735/148‑1 (MQ=255)
                      ccTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCaaa                                                                                                                        >  2:209336/1‑148 (MQ=255)
                       cTGGGGAGCGCAACTTGTCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCaaaa                                                                                                                       <  2:260274/148‑1 (MQ=255)
                       cTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCaaaa                                                                                                                       <  2:2346657/148‑1 (MQ=255)
                         ggggAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATg                                                                                                                     <  1:2466565/148‑1 (MQ=255)
                           ggAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGcc                                                                                                                   >  1:2126723/1‑148 (MQ=255)
                           ggAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGcc                                                                                                                   >  1:332295/1‑148 (MQ=255)
                            gAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGccc                                                                                                                  <  2:2162910/148‑1 (MQ=255)
                            gAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGccc                                                                                                                  >  2:1970990/1‑148 (MQ=255)
                            gAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGccc                                                                                                                  >  2:2885468/1‑148 (MQ=255)
                                 cAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCa                                                                                                             <  2:3399386/148‑1 (MQ=255)
                                 cAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCa                                                                                                             <  2:3485026/148‑1 (MQ=255)
                                 cAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCa                                                                                                             <  1:1329067/148‑1 (MQ=255)
                                    cTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATg                                                                                                          >  2:3031838/1‑148 (MQ=255)
                                     ttGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGt                                                                                                         <  2:3179985/148‑1 (MQ=255)
                                      tggctggcAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTa                                                                                                        <  1:177195/148‑1 (MQ=255)
                                        gctggcAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATg                                                                                                      <  2:978604/148‑1 (MQ=255)
                                          tggcAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATgag                                                                                                    >  2:1535557/1‑148 (MQ=255)
                                           ggcAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATgaga                                                                                                   <  2:2490046/148‑1 (MQ=255)
                                            gcAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGaa                                                                                                  <  2:2684093/148‑1 (MQ=255)
                                               gCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGa                                                                                               <  2:905869/148‑1 (MQ=255)
                                               gCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGa                                                                                               <  2:1653602/148‑1 (MQ=255)
                                                cGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGAt                                                                                              >  2:1387433/1‑148 (MQ=255)
                                                cGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGAt                                                                                              >  2:130076/1‑148 (MQ=255)
                                                 gAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATg                                                                                             <  1:217020/148‑1 (MQ=255)
                                                      ttAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTgc                                                                                        >  1:1478975/1‑148 (MQ=255)
                                                       tAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGAGCCAACGGGCGCTTCTGGATAGCTGTTATGAGTCATAACGCCTGGCAAAATGCCCATGCAATGTATTAGAACGAAGCCTgcg                                                                                       >  1:1991045/1‑148 (MQ=255)
                                                          ggCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCa                                                                                    >  2:10693/1‑148 (MQ=255)
                                                          ggCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCa                                                                                    >  2:10951/1‑148 (MQ=255)
                                                           gCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCaa                                                                                   >  2:1160828/1‑148 (MQ=255)
                                                               cTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAgcg                                                                               <  1:1966175/148‑1 (MQ=255)
                                                                ttGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAgcgc                                                                              <  2:2016476/148‑1 (MQ=255)
                                                                ttGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAgcgc                                                                              >  1:2199731/1‑148 (MQ=255)
                                                                 tGAAAATCTACAGCTTCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCt                                                                             >  1:3086823/1‑148 (MQ=255)
                                                                 tGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATTCAATGTATGAGAACGATGCCTGCGCCAAAGCGCt                                                                             <  2:686066/148‑1 (MQ=255)
                                                                 tGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCt                                                                             >  1:1701343/1‑148 (MQ=255)
                                                                      aTCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCa                                                                        >  1:2219217/1‑148 (MQ=255)
                                                                      aTCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCGTGGCa                                                                        >  1:408461/1‑148 (MQ=255)
                                                                        cTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATc                                                                      >  2:152108/1‑148 (MQ=255)
                                                                               aTCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTa                                                               <  1:3320266/148‑1 (MQ=255)
                                                                                tCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTAt                                                              >  1:369880/1‑148 (MQ=255)
                                                                                     aTGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCaa                                                         <  2:1024786/148‑1 (MQ=255)
                                                                                     aTGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCaa                                                         <  1:1274194/148‑1 (MQ=255)
                                                                                        gCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATgg                                                      <  1:1159183/148‑1 (MQ=255)
                                                                                           aagaaCGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCAAGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACAATATCTCAATGGATg                                                   >  1:3291558/1‑148 (MQ=255)
                                                                                             gaaCGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGACCGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGaa                                                 <  2:2664962/148‑1 (MQ=255)
                                                                                              aaCGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAg                                                >  2:2527079/1‑148 (MQ=255)
                                                                                                cGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGc                                              >  1:2639290/1‑148 (MQ=255)
                                                                                                   tATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATTCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATATCAATGGATGAAGGCttt                                           >  1:18221/1‑148 (MQ=255)
                                                                                                   tATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCttt                                           <  1:2992491/148‑1 (MQ=255)
                                                                                                     tCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGc                                         >  2:409580/1‑148 (MQ=255)
                                                                                                      cGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCt                                        >  1:857264/1‑148 (MQ=255)
                                                                                                         ccATGTTCATTGCTGCGCCAACGGGCGCTTCGGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCAAGCAATGTATTAGAACGATGCCTGCGCCAAATCGCTTGGCATCGACATTATATCAATGGATGAAGGCTTTGCTGTa                                     >  1:1616732/1‑148 (MQ=255)
                                                                                                           aTGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGt                                   <  2:2582941/148‑1 (MQ=255)
                                                                                                           aTGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGt                                   <  2:3137207/148‑1 (MQ=255)
                                                                                                               tCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGAcc                               <  1:2629497/148‑1 (MQ=255)
                                                                                                               tCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGAcc                               <  2:2944182/148‑1 (MQ=255)
                                                                                                                cATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCa                              <  2:3133025/148‑1 (MQ=255)
                                                                                                                   tgctgcGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGa                           <  1:464000/148‑1 (MQ=255)
                                                                                                                   tgctgcGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGa                           <  2:1744416/148‑1 (MQ=255)
                                                                                                                       gcgcCAACGGGCGCTTGTGGAGAGCTGTTATGAGTAATAATGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCAGCGCCAAAGCGCTTGGCATCGACCATATCACAAATGATGAAGGCTTTGCTGTAGTGACCATGACCGt                       >  1:3309130/1‑148 (MQ=255)
                                                                                                                       gcgcCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGt                       <  1:2243998/148‑1 (MQ=255)
                                                                                                                       gcgcCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAAGGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGt                       <  1:3259193/148‑1 (MQ=255)
                                                                                                                       gcgcCAACGGGCGCTTCTGGAGAGATGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGACCGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGt                       <  1:3387966/148‑1 (MQ=255)
                                                                                                                         gcCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCa                     <  1:2247028/148‑1 (MQ=255)
                                                                                                                            aaCGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTg                  <  1:2268560/148‑1 (MQ=255)
                                                                                                                              cGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTCGTGACCATGACCGTCACTGca                >  2:2180644/1‑148 (MQ=255)
                                                                                                                              cGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGca                >  2:2259096/1‑148 (MQ=255)
                                                                                                                               gggCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGcac               >  2:273798/1‑148 (MQ=255)
                                                                                                                                ggCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGcaca              >  2:2430360/1‑148 (MQ=255)
                                                                                                                                 gcgcTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAAGGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACaa             <  2:8186/148‑1 (MQ=255)
                                                                                                                                  cgcTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACaaa            <  2:3533832/148‑1 (MQ=255)
                                                                                                                                   gcTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAAt           <  2:1227825/148‑1 (MQ=255)
                                                                                                                                     ttCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGc         <  2:1478975/148‑1 (MQ=255)
                                                                                                                                      tCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCt        >  2:2360260/1‑148 (MQ=255)
                                                                                                                                      tCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCt        >  1:2223040/1‑148 (MQ=255)
                                                                                                                                       cTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCtt       <  2:2785550/148‑1 (MQ=255)
                                                                                                                                        tGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTa      <  2:963051/148‑1 (MQ=255)
                                                                                                                                         ggAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTaa     >  1:754882/1‑148 (MQ=255)
                                                                                                                                         ggAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTaa     >  1:2571679/1‑148 (MQ=255)
                                                                                                                                          gagaGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAAc    >  1:2203217/1‑148 (MQ=255)
                                                                                                                                                |                                                                                                                                               
AATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGG  >  CP009273/1454580‑1454867

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGTGAATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGTCATCA  >  CP009273/1454576‑1454873
                                                                                                                                                    |                                                                                                                                                     
GGTGAATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTG                                                                                                                                                      >  SRR19973066.635348/1‑150 (MQ=60)
GGTGAATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTG                                                                                                                                                      >  SRR19973066.1323859/1‑150 (MQ=60)
  TGAATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTT                                                                                                                                                    <  SRR19973066.3068654/150‑1 (MQ=60)
   GAATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTA                                                                                                                                                   >  SRR19973066.1032329/1‑150 (MQ=60)
    AATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTAT                                                                                                                                                  >  SRR19973066.1934516/1‑150 (MQ=60)
     ATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATG                                                                                                                                                 >  SRR19973066.3140259/1‑150 (MQ=60)
       TATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAG                                                                                                                                               >  SRR19973066.8322/1‑150 (MQ=60)
        ATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGT                                                                                                                                              >  SRR19973066.970118/1‑150 (MQ=60)
          CCATATCGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCA                                                                                                                                            <  SRR19973066.12279/150‑1 (MQ=60)
               TGGCCCACGTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGG                                                                                                                                       <  SRR19973066.2012008/150‑1 (MQ=60)
                 GCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCC                                                                                                                                     >  SRR19973066.2731395/1‑150 (MQ=60)
                 GCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCC                                                                                                                                     >  SRR19973066.2731339/1‑150 (MQ=60)
                 GCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCC                                                                                                                                     >  SRR19973066.2730520/1‑150 (MQ=60)
                  CCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCT                                                                                                                                    <  SRR19973066.1945734/150‑1 (MQ=60)
                   CCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTG                                                                                                                                   <  SRR19973066.694655/150‑1 (MQ=60)
                    CACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGG                                                                                                                                  <  SRR19973066.563304/150‑1 (MQ=60)
                    CACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGG                                                                                                                                  <  SRR19973066.2364374/150‑1 (MQ=60)
                    CACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGATTCATAATGCCTGG                                                                                                                                  >  SRR19973066.3248125/1‑150 (MQ=60)
                      CTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCA                                                                                                                                >  SRR19973066.1347521/1‑150 (MQ=60)
                          CCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAAT                                                                                                                            <  SRR19973066.245635/150‑1 (MQ=60)
                           CTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATG                                                                                                                           >  SRR19973066.1786746/1‑150 (MQ=60)
                            TGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGC                                                                                                                          >  SRR19973066.712512/1‑150 (MQ=60)
                             GGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCC                                                                                                                         <  SRR19973066.2483383/150‑1 (MQ=60)
                             GGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCC                                                                                                                         >  SRR19973066.334759/1‑150 (MQ=60)
                             GGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCC                                                                                                                         >  SRR19973066.334835/1‑150 (MQ=60)
                             GGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCC                                                                                                                         >  SRR19973066.2141452/1‑150 (MQ=60)
                                GAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCAT                                                                                                                      <  SRR19973066.2867638/150‑1 (MQ=60)
                                   CGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCA                                                                                                                   >  SRR19973066.2682804/1‑150 (MQ=60)
                                     CAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAAT                                                                                                                 <  SRR19973066.1338728/150‑1 (MQ=60)
                                     CAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAAT                                                                                                                 <  SRR19973066.1338702/150‑1 (MQ=60)
                                      AACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATG                                                                                                                <  SRR19973066.652140/150‑1 (MQ=60)
                                         TTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTAT                                                                                                             <  SRR19973066.3234074/150‑1 (MQ=60)
                                         TTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTAT                                                                                                             <  SRR19973066.299400/150‑1 (MQ=60)
                                          TGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATG                                                                                                            <  SRR19973066.178755/150‑1 (MQ=60)
                                             CTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTGTGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGA                                                                                                         <  SRR19973066.1209554/150‑1 (MQ=60)
                                               GGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAAC                                                                                                       <  SRR19973066.1268766/150‑1 (MQ=60)
                                                  AGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGAT                                                                                                    >  SRR19973066.2655452/1‑150 (MQ=60)
                                                     GAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCC                                                                                                 <  SRR19973066.218802/150‑1 (MQ=60)
                                                      TATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCT                                                                                                <  SRR19973066.89769/150‑1 (MQ=60)
                                                      AATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCT                                                                                                <  SRR19973066.374968/150‑1 (MQ=60)
                                                        TATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGC                                                                                              >  SRR19973066.1489375/1‑150 (MQ=60)
                                                         ATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGAGCCAACGGGCGCTTCTGGATAGCTGTTATGAGTCATAACGCCTGGCAAAATGCCCATGCAATGTATTAGAACGAAGCCTGCG                                                                                             >  SRR19973066.2005061/1‑150 (MQ=60)
                                                               GCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCAAAAG                                                                                       <  SRR19973066.3216101/150‑1 (MQ=60)
                                                                  CCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGC                                                                                    >  SRR19973066.2214985/1‑150 (MQ=60)
                                                                  CCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGC                                                                                    >  SRR19973066.2216027/1‑150 (MQ=60)
                                                                   CTTGAAAATCTACAGCTTCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCT                                                                                   >  SRR19973066.3107447/1‑150 (MQ=60)
                                                                   CTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCT                                                                                   >  SRR19973066.1713683/1‑150 (MQ=60)
                                                                   CTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCT                                                                                   >  SRR19973066.1713700/1‑150 (MQ=60)
                                                                   CTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCT                                                                                   <  SRR19973066.1980222/150‑1 (MQ=60)
                                                                   CTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCT                                                                                   <  SRR19973066.1980085/150‑1 (MQ=60)
                                                                   CTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCT                                                                                   <  SRR19973066.1980067/150‑1 (MQ=60)
                                                                     TGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTG                                                                                 <  SRR19973066.128638/150‑1 (MQ=60)
                                                                        AAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCA                                                                              >  SRR19973066.2234864/1‑150 (MQ=60)
                                                                        AAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCA                                                                              >  SRR19973066.2234574/1‑150 (MQ=60)
                                                                        AAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCA                                                                              >  SRR19973066.2461106/1‑150 (MQ=60)
                                                                        AAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCGTGGCA                                                                              >  SRR19973066.411673/1‑150 (MQ=60)
                                                                         AATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCAT                                                                             >  SRR19973066.3296103/1‑150 (MQ=60)
                                                                           TCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCG                                                                           <  SRR19973066.3344156/150‑1 (MQ=60)
                                                                            CTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTAATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGA                                                                          >  SRR19973066.1309979/1‑150 (MQ=60)
                                                                              ACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACA                                                                        <  SRR19973066.234356/150‑1 (MQ=60)
                                                                                  CATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTAT                                                                    >  SRR19973066.372778/1‑150 (MQ=60)
                                                                                   ATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATC                                                                   <  SRR19973066.3342317/150‑1 (MQ=60)
                                                                                    TCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCT                                                                  <  SRR19973066.2157655/150‑1 (MQ=60)
                                                                                    TCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCT                                                                  <  SRR19973066.2157647/150‑1 (MQ=60)
                                                                                         ATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATG                                                             <  SRR19973066.1283394/150‑1 (MQ=60)
                                                                                            GCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGAT                                                          <  SRR19973066.1167413/150‑1 (MQ=60)
                                                                                             CGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTTTGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATG                                                         >  SRR19973066.3312837/1‑150 (MQ=60)
                                                                                             CGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATG                                                         <  SRR19973066.2568154/150‑1 (MQ=60)
                                                                                             CGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATG                                                         <  SRR19973066.2568185/150‑1 (MQ=60)
                                                                                             CGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATG                                                         <  SRR19973066.3320572/150‑1 (MQ=60)
                                                                                             CGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATG                                                         <  SRR19973066.1453442/150‑1 (MQ=60)
                                                                                             CGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCAAGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACAATATCTCAATGGATG                                                         >  SRR19973066.3313276/1‑150 (MQ=60)
                                                                                               AAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATTCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAA                                                       >  SRR19973066.1347170/1‑150 (MQ=60)
                                                                                                AGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAG                                                      <  SRR19973066.1530808/150‑1 (MQ=60)
                                                                                                  AACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGC                                                    >  SRR19973066.2657050/1‑150 (MQ=60)
                                                                                                     GCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATTCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATATCAATGGATGAAGGCTTT                                                 >  SRR19973066.18497/1‑150 (MQ=60)
                                                                                                     GCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATTTATGAGAACGAGGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTT                                                 <  SRR19973066.985231/150‑1 (MQ=60)
                                                                                                     GCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTT                                                 <  SRR19973066.2689204/150‑1 (MQ=60)
                                                                                                     GCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCGTCTGGAGAGCTGTTATGAGTGATAATGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTT                                                 >  SRR19973066.18092/1‑150 (MQ=60)
                                                                                                       TATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGC                                               <  SRR19973066.3012322/150‑1 (MQ=60)
                                                                                                        ATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCACTGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCT                                              >  SRR19973066.424584/1‑150 (MQ=60)
                                                                                                        ATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCT                                              >  SRR19973066.863444/1‑150 (MQ=60)
                                                                                                        ATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCT                                              <  SRR19973066.2222336/150‑1 (MQ=60)
                                                                                                        ATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCT                                              <  SRR19973066.2222454/150‑1 (MQ=60)
                                                                                                           GCCCATGTTCATTGCTGCGCCAACGGGCGCTTGTGGAGGGCTGTTTTTATTCATAAGGCCTGGCAAAATGCCCAAGCAAAGTAATAGAACGATTCCTGCGCCAAATCCCTTGGCAACGACATTATATCAAATGATGAAGGCTTTTCTGTA                                           >  SRR19973066.1628471/1‑150 (MQ=53)
                                                                                                           GCCCATGTTCATTGCTGCGCCAACGGGCGCTTCGGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCAAGCAATGTATTAGAACGATGCCTGCGCCAAATCGCTTGGCATCGACATTATATCAATGGATGAAGGCTTTGCTGTA                                           >  SRR19973066.1628462/1‑150 (MQ=60)
                                                                                                              CATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTG                                        >  SRR19973066.1150858/1‑150 (MQ=60)
                                                                                                              CATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTG                                        >  SRR19973066.2611018/1‑150 (MQ=60)
                                                                                                               ATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATTCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGA                                       >  SRR19973066.1946432/1‑150 (MQ=60)
                                                                                                                TGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATTCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGAC                                      >  SRR19973066.1671937/1‑150 (MQ=60)
                                                                                                                 GTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATATCAATGGATGAAGGCTTTGCTGTAGTGACC                                     >  SRR19973066.3440275/1‑150 (MQ=60)
                                                                                                                   TCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCAT                                   <  SRR19973066.2647216/150‑1 (MQ=60)
                                                                                                                       TGCTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACC                               <  SRR19973066.467544/150‑1 (MQ=60)
                                                                                                                         CTGCGCCAACGGGCGCTTGTGGAGAGCTGTTATGAGTAATAATGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCAGCGCCAAAGCGCTTGGCATCGACCATATCACAAATGATGAAGGCTTTGCTGTAGTGACCATGACCGT                             >  SRR19973066.3331060/1‑150 (MQ=60)
                                                                                                                         CTGCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCAAAAGGCCAGGCAAAATGCCCATGCAATGTATGAGAACGAAGCCTGCGCCAAAGCGCGTGGCCTCGACCTTATATCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCCT                             >  SRR19973066.3331657/1‑150 (MQ=60)
                                                                                                                          TGCGCCAACGGGCGCTTCGGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTC                            >  SRR19973066.804538/1‑150 (MQ=60)
                                                                                                                           GCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCTACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCA                           <  SRR19973066.3280247/150‑1 (MQ=60)
                                                                                                                           GCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCA                           <  SRR19973066.2259476/150‑1 (MQ=60)
                                                                                                                           GCGCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAAGGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCA                           <  SRR19973066.3280631/150‑1 (MQ=60)
                                                                                                                           GCGCCAACGGGCGCTTCTGGAGAGATGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGACCGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCA                           <  SRR19973066.3410574/150‑1 (MQ=60)
                                                                                                                             GCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACT                         <  SRR19973066.2262518/150‑1 (MQ=60)
                                                                                                                             GCCAACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACT                         <  SRR19973066.2262466/150‑1 (MQ=60)
                                                                                                                                AACGGGCGCTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCA                      <  SRR19973066.2284162/150‑1 (MQ=60)
                                                                                                                                        CTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCT              <  SRR19973066.3132614/150‑1 (MQ=60)
                                                                                                                                        CTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCT              >  SRR19973066.3088296/1‑150 (MQ=60)
                                                                                                                                        CTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCT              >  SRR19973066.3382362/1‑150 (MQ=60)
                                                                                                                                        CTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCT              >  SRR19973066.2238410/1‑150 (MQ=60)
                                                                                                                                        CTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCT              >  SRR19973066.2238419/1‑150 (MQ=60)
                                                                                                                                        CTTCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCT              >  SRR19973066.2238467/1‑150 (MQ=60)
                                                                                                                                          TCTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTA            >  SRR19973066.3516297/1‑150 (MQ=60)
                                                                                                                                           CTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAA           >  SRR19973066.2589129/1‑150 (MQ=60)
                                                                                                                                           CTGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAA           >  SRR19973066.760479/1‑150 (MQ=60)
                                                                                                                                            TGGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAAC          >  SRR19973066.2218486/1‑150 (MQ=60)
                                                                                                                                             GGAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACG         <  SRR19973066.2275099/150‑1 (MQ=60)
                                                                                                                                              GAGAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGG        >  SRR19973066.1761812/1‑150 (MQ=60)
                                                                                                                                                GAGCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGTC      <  SRR19973066.2185804/150‑1 (MQ=60)
                                                                                                                                                  GCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGTCAT    <  SRR19973066.2183216/150‑1 (MQ=60)
                                                                                                                                                  GCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGTCAT    <  SRR19973066.2178662/150‑1 (MQ=60)
                                                                                                                                                  GCTGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGTCAT    <  SRR19973066.2178579/150‑1 (MQ=60)
                                                                                                                                                    TGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGTCATCA  >  SRR19973066.870248/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
GGTGAATTATCCATATGGCCCACTTGCCTGGGGAGCGCAACTTGGCTGGCAGCGAATATTAAGGCTCCTTGAAAATCTACAGCATCACTATGGCGAAGAACGCTATCGCCCATGTTCATTGCTGCGCCAACGGGCGCTTCTGGAGAGCGGTTATGAGTCATAAGGCCTGGCAAAATGCCCATGCAATGTATGAGAACGATGCCTGCGCCAAAGCGCTTGGCATCGACATTATCTCAATGGATGAAGGCTTTGCTGTAGTGACCATGACCGTCACTGCACAAATGCTTAACGGTCATCA  >  CP009273/1454576‑1454873

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑