Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,993,530 C→A 100% E113* (GAG→TAG)  fliY ← cystine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,993,5300CA98.0% 648.7 / 3.2 203E113* (GAG→TAG) fliYcystine transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (1/3);  new base A (104/95);  total (105/98)
Fisher's exact test for biased strand distribution p-value = 3.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 211 total aligned reads displayed.

TCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAACC  >  CP009273/1993385‑1993674
                                                                                                                                                 |                                                                                                                                                
tCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCtaat                                                                                                                                                <  1:281343/148‑1 (MQ=255)
tCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCtaat                                                                                                                                                >  1:250464/1‑148 (MQ=255)
  aTAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCCTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCtaataa                                                                                                                                              <  1:433628/148‑3 (MQ=255)
     gTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGaa                                                                                                                                           <  2:2952939/148‑1 (MQ=255)
        ggTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCAACAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATg                                                                                                                                        <  1:915600/148‑1 (MQ=255)
             ccAGACCGACCCCCCCTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGAc                                                                                                                                   <  2:172219/148‑1 (MQ=255)
                gaccgaccCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTg                                                                                                                                >  2:1330565/1‑148 (MQ=255)
                gaccgaccCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTg                                                                                                                                >  2:2404461/1‑148 (MQ=255)
                 accgaccCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGa                                                                                                                               >  1:262538/1‑148 (MQ=255)
                  ccgaccCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGAt                                                                                                                              <  2:2093164/148‑1 (MQ=255)
                   cgaccCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGAtt                                                                                                                             >  2:1168488/1‑148 (MQ=255)
                   cgaccCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGAtt                                                                                                                             >  1:1738114/1‑148 (MQ=255)
                    gaccCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTa                                                                                                                            <  1:941982/148‑1 (MQ=255)
                      cccccACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAAt                                                                                                                          <  1:2835812/148‑1 (MQ=255)
                      cccccACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAAt                                                                                                                          >  2:3234938/1‑148 (MQ=255)
                       ccccACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATc                                                                                                                         <  2:1291231/148‑1 (MQ=255)
                        cccACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATca                                                                                                                        <  2:1076723/148‑1 (MQ=255)
                          cACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATcacc                                                                                                                      <  2:250464/148‑1 (MQ=255)
                            cTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATcaccac                                                                                                                    >  1:2486282/1‑148 (MQ=255)
                                 ttGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCaa                                                                                                               <  2:3037373/148‑1 (MQ=255)
                                   gCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATa                                                                                                             <  1:794011/148‑1 (MQ=255)
                                      tttCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGt                                                                                                          <  1:2171930/148‑1 (MQ=255)
                                      tttCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGt                                                                                                          >  2:3390086/1‑148 (MQ=255)
                                      tttCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGt                                                                                                          <  2:1033815/148‑1 (MQ=255)
                                        tCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGttt                                                                                                        >  1:2949115/1‑148 (MQ=255)
                                           gATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTaga                                                                                                     >  1:2023188/1‑148 (MQ=255)
                                           gATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTaga                                                                                                     <  1:1161290/148‑1 (MQ=255)
                                              catcGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGACATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTc                                                                                                  >  1:1902804/1‑148 (MQ=255)
                                              catcGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTc                                                                                                  <  2:2253434/148‑1 (MQ=255)
                                              catcGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTc                                                                                                  <  1:3506484/148‑1 (MQ=255)
                                               atcGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTGGAGTcc                                                                                                 >  1:2952708/1‑148 (MQ=255)
                                               atcGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTcc                                                                                                 <  2:297402/148‑1 (MQ=255)
                                               atcGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTcc                                                                                                 >  2:2702594/1‑148 (MQ=255)
                                                tcGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCa                                                                                                <  1:301420/148‑1 (MQ=255)
                                                tcGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCa                                                                                                >  1:1011042/1‑148 (MQ=255)
                                                 cGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAg                                                                                               >  1:3150937/1‑148 (MQ=255)
                                                 cGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAg                                                                                               <  2:3540542/148‑1 (MQ=255)
                                                   gCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCGAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCg                                                                                             <  2:1149903/148‑1 (MQ=255)
                                                    cTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGa                                                                                            >  2:3352603/1‑148 (MQ=255)
                                                       ttttAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGc                                                                                         <  2:1365935/148‑1 (MQ=255)
                                                        tttAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGcc                                                                                        <  1:1592641/148‑1 (MQ=255)
                                                        tttAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGAGCTAATCAGAAATGGTGACCTGATTAAACACCACATCAATACGTTTAGATTCCAGCGACGcc                                                                                        >  1:2902043/1‑148 (MQ=255)
                                                          tAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAg                                                                                      >  1:177955/1‑148 (MQ=255)
                                                             tGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCAt                                                                                   >  1:1087916/1‑148 (MQ=255)
                                                                tGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATAcc                                                                                >  1:775867/1‑148 (MQ=255)
                                                                  ccTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGt                                                                              >  2:610981/1‑148 (MQ=255)
                                                                   cTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTc                                                                             >  1:1370885/1‑148 (MQ=255)
                                                                    ttCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTcc                                                                            >  1:3538832/1‑148 (MQ=255)
                                                                    ttCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTcc                                                                            <  1:2298847/148‑1 (MQ=255)
                                                                      cGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCa                                                                          >  1:1770915/1‑148 (MQ=255)
                                                                       gTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCAt                                                                         <  2:1531092/148‑1 (MQ=255)
                                                                       gTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCAt                                                                         <  2:492550/148‑1 (MQ=255)
                                                                       gTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCAt                                                                         <  2:1528407/148‑1 (MQ=255)
                                                                         tACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCAttt                                                                       >  2:3215012/1‑148 (MQ=255)
                                                                          aCCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTg                                                                      >  2:435247/1‑148 (MQ=255)
                                                                           ccTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTgg                                                                     >  1:712657/1‑148 (MQ=255)
                                                                           ccTTTTGTCACCAGCGCCTGAAGACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTgg                                                                     <  2:1850079/148‑1 (MQ=255)
                                                                             ttttttCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTc                                                                   >  1:1051272/1‑148 (MQ=255)
                                                                             ttttttCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTc                                                                   >  2:124013/1‑148 (MQ=255)
                                                                              tttttCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCg                                                                  >  1:628626/1‑148 (MQ=255)
                                                                                    aCCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTa                                                            >  1:703076/1‑148 (MQ=255)
                                                                                       aGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTg                                                         <  1:1330565/148‑1 (MQ=255)
                                                                                          gcCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACg                                                      <  2:1632046/148‑1 (MQ=255)
                                                                                           ccTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGc                                                     <  2:177955/148‑1 (MQ=255)
                                                                                            cTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGcc                                                    <  2:734543/148‑1 (MQ=255)
                                                                                            cTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGcc                                                    <  1:1168488/148‑1 (MQ=255)
                                                                                             tGAATACCAGAAATGGTGTACGGGGTTGAGAAATCTTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCt                                                   <  1:330020/148‑1 (MQ=255)
                                                                                             tGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCt                                                   <  1:220260/148‑1 (MQ=255)
                                                                                             tGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCt                                                   <  2:1624610/148‑1 (MQ=255)
                                                                                             tGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCt                                                   <  2:712657/148‑1 (MQ=255)
                                                                                             tGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCt                                                   <  1:666999/148‑1 (MQ=255)
                                                                                             tGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCt                                                   >  2:2387433/1‑148 (MQ=255)
                                                                                              gAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTc                                                  >  1:1151675/1‑148 (MQ=255)
                                                                                               aaTACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCa                                                 >  2:110395/1‑148 (MQ=255)
                                                                                                aTACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCaa                                                <  1:876507/148‑1 (MQ=255)
                                                                                                 tACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAAc                                               >  2:1574722/1‑148 (MQ=255)
                                                                                                  aCCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCACTACGTTTAGAGTCCAGCTACGCCAGCATACCGTCCCATGTGGTCGGTGTTAGTGACGCCTCATCg                                              >  1:3336028/1‑148 (MQ=255)
                                                                                                  aCCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACg                                              >  2:1066201/1‑148 (MQ=255)
                                                                                                     aGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCa                                           >  1:1249077/1‑148 (MQ=255)
                                                                                                      gAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCaa                                          >  1:3278770/1‑148 (MQ=255)
                                                                                                      gAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCaa                                          >  1:2910510/1‑148 (MQ=255)
                                                                                                        aaTGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGa                                        <  2:1738114/148‑1 (MQ=255)
                                                                                                         aTGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGAt                                       <  2:1779764/148‑1 (MQ=255)
                                                                                                          tGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATg                                      >  1:3142682/1‑148 (MQ=255)
                                                                                                           ggTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGt                                     <  2:65634/148‑1 (MQ=255)
                                                                                                           ggTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGt                                     >  2:1971936/1‑148 (MQ=255)
                                                                                                               tACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTg                                 <  2:597966/148‑1 (MQ=255)
                                                                                                                 cGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGcc                               <  2:2486282/148‑1 (MQ=255)
                                                                                                                    ggTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGc                            >  2:2946662/1‑148 (MQ=255)
                                                                                                                       tGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGt                         >  1:2393666/1‑148 (MQ=255)
                                                                                                                       tGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGt                         >  1:3344915/1‑148 (MQ=255)
                                                                                                                        gagaAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGtt                        <  2:3150937/148‑1 (MQ=255)
                                                                                                                             aTCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCa                   >  2:1222146/1‑148 (MQ=255)
                                                                                                                               cGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCaaa                 >  1:504773/1‑148 (MQ=255)
                                                                                                                                  aTTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTc              >  2:3519486/1‑148 (MQ=255)
                                                                                                                                      tttCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACt          <  2:139347/148‑1 (MQ=255)
                                                                                                                                           tGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCaaa     >  1:926851/1‑148 (MQ=255)
                                                                                                                                           tGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCaaa     <  2:2553906/148‑1 (MQ=255)
                                                                                                                                            gcgcTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGATGTTGGGCAAATTCCACTTCaaaa    <  1:1104357/148‑1 (MQ=255)
                                                                                                                                              gcTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAAcc  <  1:2554567/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
TCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAACC  >  CP009273/1993385‑1993674

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTCTTCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAACCGGTTA  >  CP009273/1993381‑1993679
                                                                                                                                                     |                                                                                                                                                     
CTCTTCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTA                                                                                                                                                       >  SRR19973066.2571252/1‑150 (MQ=60)
CTCTTCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTA                                                                                                                                                       >  SRR19973066.2571250/1‑150 (MQ=60)
  CTTCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAAT                                                                                                                                                     >  SRR19973066.252387/1‑150 (MQ=60)
  CTTCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAAT                                                                                                                                                     >  SRR19973066.252269/1‑150 (MQ=60)
    TCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGACATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCA                                                                                                                                                   >  SRR19973066.785189/1‑150 (MQ=60)
    TCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCA                                                                                                                                                   <  SRR19973066.283939/150‑1 (MQ=60)
    TCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCA                                                                                                                                                   <  SRR19973066.283388/150‑1 (MQ=60)
    TCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCA                                                                                                                                                   <  SRR19973066.1600286/150‑1 (MQ=60)
      ATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGA                                                                                                                                                 >  SRR19973066.140692/1‑150 (MQ=60)
      ATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCCTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATAAGA                                                                                                                                                 <  SRR19973066.436985/150‑1 (MQ=60)
          TTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATG                                                                                                                                             <  SRR19973066.2543806/150‑1 (MQ=60)
            GGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCAACAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGT                                                                                                                                           <  SRR19973066.922138/150‑1 (MQ=60)
                 CCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCT                                                                                                                                      <  SRR19973066.1076355/150‑1 (MQ=60)
                  CAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTG                                                                                                                                     <  SRR19973066.1054412/150‑1 (MQ=60)
                   AGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGA                                                                                                                                    >  SRR19973066.264508/1‑150 (MQ=60)
                     ACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATT                                                                                                                                  >  SRR19973066.1750658/1‑150 (MQ=60)
                        GACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAAT                                                                                                                               <  SRR19973066.948847/150‑1 (MQ=60)
                          CCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCA                                                                                                                             <  SRR19973066.2854871/150‑1 (MQ=60)
                           CCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCAC                                                                                                                            >  SRR19973066.1763205/1‑150 (MQ=60)
                            CCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACC                                                                                                                           >  SRR19973066.782969/1‑150 (MQ=60)
                              CACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCAC                                                                                                                         >  SRR19973066.2630563/1‑150 (MQ=60)
                              CACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCAC                                                                                                                         >  SRR19973066.810425/1‑150 (MQ=60)
                              CACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCAC                                                                                                                         >  SRR19973066.2503243/1‑150 (MQ=60)
                               ACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACA                                                                                                                        >  SRR19973066.917247/1‑150 (MQ=60)
                                       GCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACG                                                                                                                <  SRR19973066.799843/150‑1 (MQ=60)
                                          TTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTT                                                                                                             >  SRR19973066.2970340/1‑150 (MQ=60)
                                          TTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTT                                                                                                             >  SRR19973066.2968683/1‑150 (MQ=60)
                                          TTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTT                                                                                                             <  SRR19973066.2187186/150‑1 (MQ=60)
                                          TTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTT                                                                                                             <  SRR19973066.2187013/150‑1 (MQ=60)
                                            TCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAG                                                                                                           >  SRR19973066.1372006/1‑150 (MQ=60)
                                             CAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGA                                                                                                          >  SRR19973066.2037334/1‑150 (MQ=60)
                                             CAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAAAACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCATAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGA                                                                                                          >  SRR19973066.2254077/1‑150 (MQ=60)
                                               GATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGT                                                                                                        <  SRR19973066.1169527/150‑1 (MQ=60)
                                               GATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGT                                                                                                        <  SRR19973066.1170887/150‑1 (MQ=60)
                                                ATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGACATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTC                                                                                                       >  SRR19973066.1916371/1‑150 (MQ=60)
                                                 CCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTGGAGTCC                                                                                                      >  SRR19973066.2972290/1‑150 (MQ=60)
                                                  CATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCA                                                                                                     <  SRR19973066.3529854/150‑1 (MQ=60)
                                                  CATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCA                                                                                                     >  SRR19973066.1018494/1‑150 (MQ=60)
                                                   ATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAG                                                                                                    >  SRR19973066.3171942/1‑150 (MQ=60)
                                                    TCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGC                                                                                                   <  SRR19973066.303556/150‑1 (MQ=60)
                                                    TCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGC                                                                                                   <  SRR19973066.2004954/150‑1 (MQ=60)
                                                       GCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGAC                                                                                                <  SRR19973066.3535276/150‑1 (MQ=60)
                                                          GTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCC                                                                                             >  SRR19973066.2921387/1‑150 (MQ=60)
                                                          GTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGAGCTAATCAGAAATGGTGACCTGATTAAACACCACATCAATACGTTTAGATTCCAGCGACGCC                                                                                             >  SRR19973066.2921431/1‑150 (MQ=60)
                                                            TTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAG                                                                                           >  SRR19973066.179522/1‑150 (MQ=60)
                                                            TTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAG                                                                                           >  SRR19973066.179519/1‑150 (MQ=60)
                                                            TTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAG                                                                                           <  SRR19973066.1604170/150‑1 (MQ=60)
                                                            TTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAG                                                                                           >  SRR19973066.179190/1‑150 (MQ=60)
                                                              TAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCA                                                                                         >  SRR19973066.442254/1‑150 (MQ=60)
                                                               AATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCAT                                                                                        >  SRR19973066.1095851/1‑150 (MQ=60)
                                                                ATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATA                                                                                       <  SRR19973066.3343026/150‑1 (MQ=60)
                                                                 TGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATAC                                                                                      >  SRR19973066.3512839/1‑150 (MQ=60)
                                                                  GGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACC                                                                                     <  SRR19973066.934876/150‑1 (MQ=60)
                                                                  GGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACC                                                                                     >  SRR19973066.781603/1‑150 (MQ=60)
                                                                     GCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTC                                                                                  >  SRR19973066.1380804/1‑150 (MQ=60)
                                                                      CCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCC                                                                                 >  SRR19973066.3562351/1‑150 (MQ=60)
                                                                        TTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCA                                                                               >  SRR19973066.1783597/1‑150 (MQ=60)
                                                                        TTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCA                                                                               <  SRR19973066.2314605/150‑1 (MQ=60)
                                                                           GTTACCTTTTTTCACCCGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTT                                                                            >  SRR19973066.686287/1‑150 (MQ=60)
                                                                           GTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTT                                                                            >  SRR19973066.2938130/1‑150 (MQ=60)
                                                                            TTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTTACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTG                                                                           >  SRR19973066.922269/1‑150 (MQ=60)
                                                                             TACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGG                                                                          >  SRR19973066.717937/1‑150 (MQ=60)
                                                                               CCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTC                                                                        >  SRR19973066.1059002/1‑150 (MQ=60)
                                                                                CTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCG                                                                       >  SRR19973066.633137/1‑150 (MQ=60)
                                                                                     TTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTT                                                                  <  SRR19973066.1164125/150‑1 (MQ=60)
                                                                                      TCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTA                                                                 >  SRR19973066.708331/1‑150 (MQ=60)
                                                                                      TCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTA                                                                 >  SRR19973066.708264/1‑150 (MQ=60)
                                                                                         CCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTG                                                              <  SRR19973066.1102452/150‑1 (MQ=60)
                                                                                           AGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGAC                                                            <  SRR19973066.2336320/150‑1 (MQ=60)
                                                                                           AGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGAC                                                            <  SRR19973066.1340215/150‑1 (MQ=60)
                                                                                               CCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCT                                                        >  SRR19973066.359574/1‑150 (MQ=60)
                                                                                                CTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTC                                                       >  SRR19973066.2940150/1‑150 (MQ=60)
                                                                                                CTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTC                                                       <  SRR19973066.1176750/150‑1 (MQ=60)
                                                                                                CTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTC                                                       >  SRR19973066.1159881/1‑150 (MQ=60)
                                                                                                CTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTC                                                       >  SRR19973066.1159817/1‑150 (MQ=60)
                                                                                                 TGAATACCAGAAATGGTGTACGGGGTTGAGAAATCTTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCA                                                      <  SRR19973066.332534/150‑1 (MQ=60)
                                                                                                 TGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCA                                                      <  SRR19973066.222054/150‑1 (MQ=60)
                                                                                                 TGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCA                                                      <  SRR19973066.671872/150‑1 (MQ=60)
                                                                                                    ATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCACTACGTTTAGAGTCCAGCTACGCCAGCATACCGTCCCATGTGGTCGGTGTTAGTGACGCCTCATCG                                                   >  SRR19973066.3358225/1‑150 (MQ=60)
                                                                                                    ATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACG                                                   >  SRR19973066.3358263/1‑150 (MQ=60)
                                                                                                    ATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACG                                                   <  SRR19973066.882760/150‑1 (MQ=60)
                                                                                                    ATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACG                                                   <  SRR19973066.2420718/150‑1 (MQ=60)
                                                                                                    ATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACG                                                   <  SRR19973066.2680505/150‑1 (MQ=60)
                                                                                                      ACCAGAAATGGTGTACGGGGTTGACAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCC                                                 <  SRR19973066.1216550/150‑1 (MQ=60)
                                                                                                       CCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCA                                                >  SRR19973066.1258044/1‑150 (MQ=60)
                                                                                                        CAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAA                                               >  SRR19973066.3300304/1‑150 (MQ=60)
                                                                                                        CAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAA                                               >  SRR19973066.1333249/1‑150 (MQ=60)
                                                                                                        CAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAA                                               >  SRR19973066.3300553/1‑150 (MQ=60)
                                                                                                        CAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAA                                               >  SRR19973066.2929940/1‑150 (MQ=60)
                                                                                                           AAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGAT                                            <  SRR19973066.75917/150‑1 (MQ=60)
                                                                                                            AATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATG                                           >  SRR19973066.3163663/1‑150 (MQ=60)
                                                                                                            AATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATG                                           >  SRR19973066.3163646/1‑150 (MQ=60)
                                                                                                               GGTGTACGGGGTTGAGAAATCTTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTT                                        <  SRR19973066.640721/150‑1 (MQ=60)
                                                                                                                   TACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCC                                    >  SRR19973066.3104783/1‑150 (MQ=60)
                                                                                                                       GGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCT                                <  SRR19973066.1745280/150‑1 (MQ=60)
                                                                                                                         GTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGT                              >  SRR19973066.2409785/1‑150 (MQ=60)
                                                                                                                         GTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGT                              >  SRR19973066.2408550/1‑150 (MQ=60)
                                                                                                                         GTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGT                              >  SRR19973066.3367185/1‑150 (MQ=60)
                                                                                                                         GTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGT                              >  SRR19973066.2147529/1‑150 (MQ=60)
                                                                                                                           TGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTG                            >  SRR19973066.2927271/1‑150 (MQ=60)
                                                                                                                             AGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGG                          >  SRR19973066.606157/1‑150 (MQ=60)
                                                                                                                              GAAATCGTATTTTTTCTTGCGCCAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGC                         >  SRR19973066.3257470/1‑150 (MQ=60)
                                                                                                                                 ATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAA                      >  SRR19973066.3016600/1‑150 (MQ=60)
                                                                                                                                 ATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAA                      >  SRR19973066.508524/1‑150 (MQ=60)
                                                                                                                                  TCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAAT                     <  SRR19973066.3439408/150‑1 (MQ=60)
                                                                                                                                             CTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAA          >  SRR19973066.933557/1‑150 (MQ=60)
                                                                                                                                                GCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGATGTTGGGCAAATTCCACTTCAAAACC       <  SRR19973066.1112362/150‑1 (MQ=60)
                                                                                                                                                  GCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAACCGG     <  SRR19973066.2571914/150‑1 (MQ=60)
                                                                                                                                                    TAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAACCGGTT   <  SRR19973066.1380419/150‑1 (MQ=60)
                                                                                                                                                     AATCAGAAATGGTGACCTGATTAATCACCACATCACTACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAACCGGTTA  <  SRR19973066.2107385/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
CTCTTCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAACCGGTTA  >  CP009273/1993381‑1993679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑