Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,993,530 C→A 100% E113* (GAG→TAG)  fliY ← cystine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,993,5300CA98.2% 686.8 / 1.7 214E113* (GAG→TAG) fliYcystine transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (1/3);  new base A (109/101);  total (110/104)
Fisher's exact test for biased strand distribution p-value = 3.58e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.99e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 219 total aligned reads displayed.

TCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAAC  >  CP009273/1993385‑1993673
                                                                                                                                                 |                                                                                                                                               
tCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCtaat                                                                                                                                               >  2:1043772/1‑148 (MQ=255)
   tAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAg                                                                                                                                            >  2:253883/1‑148 (MQ=255)
    aGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGa                                                                                                                                           >  2:1687404/1‑148 (MQ=255)
      ttGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGaaa                                                                                                                                         <  2:3242588/148‑1 (MQ=255)
                 accgaccCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGa                                                                                                                              >  1:3204911/1‑148 (MQ=255)
                 accgaccCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGa                                                                                                                              <  1:1286213/148‑1 (MQ=255)
                 accgaccCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGa                                                                                                                              <  1:2102204/148‑1 (MQ=255)
                  ccgaccCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGAt                                                                                                                             >  1:1278094/1‑148 (MQ=255)
                  ccgaccCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGAt                                                                                                                             >  1:2862759/1‑148 (MQ=255)
                     accCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTaa                                                                                                                          <  2:1494693/148‑1 (MQ=255)
                     acacccACTTTTTTGCCTTTCAGATAATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTaa                                                                                                                          >  2:3167671/4‑148 (MQ=255)
                      cccccACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAAt                                                                                                                         <  2:248547/148‑1 (MQ=255)
                        cccACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATca                                                                                                                       >  1:646052/1‑148 (MQ=255)
                         ccACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATcac                                                                                                                      <  1:429587/148‑1 (MQ=255)
                         ccACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATcac                                                                                                                      >  2:146201/1‑148 (MQ=255)
                         ccACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATcac                                                                                                                      <  1:326531/148‑1 (MQ=255)
                         ccACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATcac                                                                                                                      <  1:432139/148‑1 (MQ=255)
                          cACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATcacc                                                                                                                     <  2:196692/148‑1 (MQ=255)
                           aCTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATcacca                                                                                                                    >  2:2478455/1‑148 (MQ=255)
                            cTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATcaccac                                                                                                                   >  1:2617457/1‑148 (MQ=255)
                                 ttGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCaa                                                                                                              <  2:39157/148‑1 (MQ=255)
                                 ttGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCaa                                                                                                              <  2:3450252/148‑1 (MQ=255)
                                  tGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGNTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAAt                                                                                                             <  1:1785755/148‑1 (MQ=255)
                                     cTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACg                                                                                                          >  2:735160/1‑148 (MQ=255)
                                      tttCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGt                                                                                                         >  2:2526662/1‑148 (MQ=255)
                                      tttCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGt                                                                                                         >  2:2526641/1‑148 (MQ=255)
                                      tttCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGt                                                                                                         <  1:2704189/148‑1 (MQ=255)
                                       ttCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGtt                                                                                                        <  2:340801/148‑1 (MQ=255)
                                       ttCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGtt                                                                                                        <  2:1665111/148‑1 (MQ=255)
                                           gATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTaga                                                                                                    >  2:1627066/1‑148 (MQ=255)
                                           gATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTaga                                                                                                    >  1:3380085/1‑148 (MQ=255)
                                           gATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTaga                                                                                                    <  2:1211265/148‑1 (MQ=255)
                                            atcatcGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTagag                                                                                                   >  1:3412166/1‑148 (MQ=255)
                                             tcatcGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGt                                                                                                  >  1:3097140/1‑148 (MQ=255)
                                              catcGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTc                                                                                                 >  1:2916727/1‑148 (MQ=255)
                                                 cGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAg                                                                                              >  2:1012821/1‑148 (MQ=255)
                                                  ggCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGc                                                                                             <  2:3247288/148‑1 (MQ=255)
                                                    cTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGa                                                                                           >  2:2091911/1‑148 (MQ=255)
                                                     tGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGt                                                                                                         <  1:3502650/133‑1 (MQ=255)
                                                      gTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACg                                                                                         <  2:3261971/148‑1 (MQ=255)
                                                       ttttAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGc                                                                                        >  2:367130/1‑148 (MQ=255)
                                                        tttAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGcc                                                                                       <  1:1905679/148‑1 (MQ=255)
                                                        tttAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGcc                                                                                       >  1:1314008/1‑148 (MQ=255)
                                                        tttAATGGTGCCTTCGTTACCTTTTTTCACCAGAGCCTGATTACCTTAAATGGTGTACGTGGTTGATAAATCTTATTTTTTCTTGCGCTAATCTGAATTGGTTACATGATTAATCACCACATCATTACGTTTAGTGTCCAGCGACGcc                                                                                       <  1:922864/148‑1 (MQ=255)
                                                              ggTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATa                                                                                 <  1:2889063/148‑1 (MQ=255)
                                                                  ccTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGt                                                                             <  1:24837/148‑1 (MQ=255)
                                                                    ttCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTcc                                                                           >  2:691170/1‑148 (MQ=255)
                                                                    ttCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTcc                                                                           >  1:9597/1‑148 (MQ=255)
                                                                     tCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTccc                                                                          <  2:2022022/148‑1 (MQ=255)
                                                                        ttACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCAtt                                                                       <  1:729021/148‑1 (MQ=255)
                                                                          aCCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTg                                                                     >  2:1878705/1‑148 (MQ=255)
                                                                           ccTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCAGTTgg                                                                    >  2:2865154/1‑148 (MQ=255)
                                                                              tttttCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCg                                                                 >  2:1068999/1‑148 (MQ=255)
                                                                               ttttCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCgg                                                                <  2:523472/148‑1 (MQ=255)
                                                                                 ttCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGtt                                                              >  2:1068862/1‑148 (MQ=255)
                                                                                     ccAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAg                                                          >  2:1182229/1‑148 (MQ=255)
                                                                                     ccAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAg                                                          <  2:3341581/148‑1 (MQ=255)
                                                                                     ccAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAg                                                          >  1:2065691/1‑148 (MQ=255)
                                                                                     ccAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAg                                                          <  1:1158931/148‑1 (MQ=255)
                                                                                       aGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTg                                                        <  2:622155/148‑1 (MQ=255)
                                                                                         cgcCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGAc                                                      <  2:2854344/148‑1 (MQ=255)
                                                                                            cTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGcc                                                   >  2:2453254/1‑148 (MQ=255)
                                                                                             tGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCt                                                  >  1:1667478/1‑148 (MQ=255)
                                                                                               aaTACCACAAATGGTGTACGGGGTTTAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATATGGTCGGTGTTAGTGACGCCTCa                                                >  2:3475708/1‑148 (MQ=255)
                                                                                                aTACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCaa                                               >  2:3404355/1‑148 (MQ=255)
                                                                                                aTACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCaa                                               >  2:370535/1‑148 (MQ=255)
                                                                                                  aCCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACg                                             >  2:2720462/1‑148 (MQ=255)
                                                                                                      gAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCaa                                         >  1:577758/1‑148 (MQ=255)
                                                                                                       aaaTGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACTCCAAg                                        >  2:2830504/1‑148 (MQ=255)
                                                                                                       aaaTGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAg                                        >  2:714801/1‑148 (MQ=255)
                                                                                                           ggTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGt                                    <  1:1854315/148‑1 (MQ=255)
                                                                                                            gtgtACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGtt                                   <  1:2707171/148‑1 (MQ=255)
                                                                                                            gtgtACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGtt                                   <  1:253883/148‑1 (MQ=255)
                                                                                                              gtACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGtttt                                 <  1:2187717/148‑1 (MQ=255)
                                                                                                               tACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTg                                <  2:2251149/148‑1 (MQ=255)
                                                                                                                aCGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATANCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGc                               <  2:79671/148‑1 (MQ=255)
                                                                                                                  ggggTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCa                             >  1:2094029/1‑148 (MQ=255)
                                                                                                                    ggTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGc                           <  1:2041455/148‑1 (MQ=255)
                                                                                                                     gTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCt                          <  1:1627066/148‑1 (MQ=255)
                                                                                                                     gTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCt                          <  1:146201/148‑1 (MQ=255)
                                                                                                                      ttGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTg                         <  1:1687404/148‑1 (MQ=255)
                                                                                                                      ttGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTg                         <  1:3007006/148‑1 (MQ=255)
                                                                                                                      ttGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTg                         >  2:2229368/1‑148 (MQ=255)
                                                                                                                       tGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGt                        <  1:673134/148‑1 (MQ=255)
                                                                                                                        gagaAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGtt                       <  2:1278094/148‑1 (MQ=255)
                                                                                                                        gagaAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGtt                       <  2:3078064/148‑1 (MQ=255)
                                                                                                                        gagaAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGtt                       <  2:1295720/148‑1 (MQ=255)
                                                                                                                           aaaTCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTggg                    >  1:2554791/1‑148 (MQ=255)
                                                                                                                           aaaTCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTggg                    >  1:797776/1‑148 (MQ=255)
                                                                                                                           aaaTCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTggg                    >  2:903941/1‑148 (MQ=255)
                                                                                                                            aaTCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGc                   >  1:1776510/1‑148 (MQ=255)
                                                                                                                            aaTCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGc                   >  1:2701370/1‑148 (MQ=255)
                                                                                                                               cGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCaaa                >  1:3349963/1‑148 (MQ=255)
                                                                                                                                 tATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAAtt              >  2:2226854/1‑148 (MQ=255)
                                                                                                                                   ttttttCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTcc            >  2:313801/1‑148 (MQ=255)
                                                                                                                                       ttCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACtt        >  2:1117876/1‑148 (MQ=255)
                                                                                                                                         cTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCa      >  1:2440429/1‑148 (MQ=255)
                                                                                                                                           tGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCaaa    <  2:2648134/148‑1 (MQ=255)
                                                                                                                                            gcgcTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCaaaa   <  1:368558/148‑1 (MQ=255)
                                                                                                                                             cgcTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAAc  >  1:3021183/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
TCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAAC  >  CP009273/1993385‑1993673

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTTCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAACCGGTTA  >  CP009273/1993383‑1993679
                                                                                                                                                   |                                                                                                                                                     
CTTCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAAT                                                                                                                                                     <  SRR19973065.1592544/150‑1 (MQ=60)
CTTCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAAT                                                                                                                                                     <  SRR19973065.1592498/150‑1 (MQ=60)
 TTCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATC                                                                                                                                                    >  SRR19973065.1928532/1‑150 (MQ=60)
  TCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCA                                                                                                                                                   <  SRR19973065.365974/150‑1 (MQ=60)
   CATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAG                                                                                                                                                  >  SRR19973065.311695/1‑150 (MQ=60)
      AGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAA                                                                                                                                               >  SRR19973065.462812/1‑150 (MQ=60)
       GTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAAT                                                                                                                                              >  SRR19973065.2981282/1‑150 (MQ=60)
                CAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGNGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTG                                                                                                                                     >  SRR19973065.1795063/1‑150 (MQ=60)
                CAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTG                                                                                                                                     >  SRR19973065.2896456/1‑150 (MQ=60)
                CAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTG                                                                                                                                     >  SRR19973065.586912/1‑150 (MQ=60)
                 AGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGA                                                                                                                                    >  SRR19973065.3224202/1‑150 (MQ=60)
                  GACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGAT                                                                                                                                   >  SRR19973065.1286529/1‑150 (MQ=60)
                  GACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGAT                                                                                                                                   >  SRR19973065.1286640/1‑150 (MQ=60)
                  GACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGAT                                                                                                                                   >  SRR19973065.1286533/1‑150 (MQ=60)
                  GACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGAT                                                                                                                                   >  SRR19973065.2880331/1‑150 (MQ=60)
                   ACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATT                                                                                                                                  <  SRR19973065.754244/150‑1 (MQ=60)
                   ACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATT                                                                                                                                  <  SRR19973065.2115720/150‑1 (MQ=60)
                   ACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATT                                                                                                                                  <  SRR19973065.1294693/150‑1 (MQ=60)
                        CCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCA                                                                                                                             >  SRR19973065.650299/1‑150 (MQ=60)
                        CCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCA                                                                                                                             <  SRR19973065.3240988/150‑1 (MQ=60)
                           CCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCA                                                                                                                          <  SRR19973065.435144/150‑1 (MQ=60)
                           CCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCA                                                                                                                          <  SRR19973065.433551/150‑1 (MQ=60)
                           CCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCA                                                                                                                          <  SRR19973065.2110591/150‑1 (MQ=60)
                           CCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCA                                                                                                                          <  SRR19973065.432568/150‑1 (MQ=60)
                           CCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCA                                                                                                                          <  SRR19973065.328804/150‑1 (MQ=60)
                            CACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCAC                                                                                                                         >  SRR19973065.2633669/1‑150 (MQ=60)
                                  TTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAAT                                                                                                                   <  SRR19973065.1340775/150‑1 (MQ=60)
                                    TGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGNTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATAC                                                                                                                 <  SRR19973065.1797441/150‑1 (MQ=60)
                                        gctcttccgatctGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTT                                                                                                             <  SRR19973065.3523920/137‑1 (MQ=60)
                                        TTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTT                                                                                                             <  SRR19973065.2721007/150‑1 (MQ=60)
                                        TTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTT                                                                                                             <  SRR19973065.2720996/150‑1 (MQ=60)
                                        TTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTT                                                                                                             <  SRR19973065.2720989/150‑1 (MQ=60)
                                         TTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTA                                                                                                            >  SRR19973065.322369/1‑150 (MQ=60)
                                           CAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGA                                                                                                          >  SRR19973065.3400750/1‑150 (MQ=60)
                                           CAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGA                                                                                                          >  SRR19973065.2672474/1‑150 (MQ=60)
                                           CAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGA                                                                                                          >  SRR19973065.3400192/1‑150 (MQ=60)
                                            AGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAG                                                                                                         >  SRR19973065.1679790/1‑150 (MQ=60)
                                            AGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAG                                                                                                         <  SRR19973065.1672276/150‑1 (MQ=60)
                                            AGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAG                                                                                                         >  SRR19973065.3433012/1‑150 (MQ=60)
                                            AGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAG                                                                                                         <  SRR19973065.709794/150‑1 (MQ=60)
                                             GATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGT                                                                                                        >  SRR19973065.3116055/1‑150 (MQ=60)
                                              ATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTC                                                                                                       >  SRR19973065.2934468/1‑150 (MQ=60)
                                               TCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCC                                                                                                      >  SRR19973065.1865950/1‑150 (MQ=60)
                                                    GGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGA                                                                                                 >  SRR19973065.2609851/1‑150 (MQ=60)
                                                        GTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCC                                                                                             >  SRR19973065.1322698/1‑150 (MQ=60)
                                                          TTTATTGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAG                                                                                           <  SRR19973065.1918199/150‑1 (MQ=60)
                                                          TTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAG                                                                                           <  SRR19973065.245542/150‑1 (MQ=60)
                                                          TTTAATGGTGCCTTCGTTACCTTTTTTCACCAGAGCCTGATTACCTTAAATGGTGTACGTGGTTGATAAATCTTATTTTTTCTTGCGCTAATCTGAATTGGTTACATGATTAATCACCACATCATTACGTTTAGTGTCCAGCGACGCCAG                                                                                           <  SRR19973065.928911/150‑1 (MQ=60)
                                                                GGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACC                                                                                     <  SRR19973065.2906720/150‑1 (MQ=60)
                                                                   GCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTC                                                                                  >  SRR19973065.3519788/1‑150 (MQ=60)
                                                                    CCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCC                                                                                 >  SRR19973065.9749/1‑150 (MQ=60)
                                                                    CCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCC                                                                                 <  SRR19973065.25169/150‑1 (MQ=60)
                                                                    CCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCC                                                                                 <  SRR19973065.25168/150‑1 (MQ=60)
                                                                     CTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCC                                                                                <  SRR19973065.1308932/150‑1 (MQ=60)
                                                                          TTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTG                                                                           <  SRR19973065.733903/150‑1 (MQ=60)
                                                                          TTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTG                                                                           >  SRR19973065.3346682/1‑150 (MQ=60)
                                                                            ACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGTGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGGGTGGCCAGCATACCGTCCCATTTGGT                                                                         <  SRR19973065.2861878/150‑1 (MQ=60)
                                                                              CTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACTCCAGCATACCGTCCCATTTGGTCG                                                                       >  SRR19973065.672578/1‑150 (MQ=60)
                                                                                TTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGT                                                                     <  SRR19973065.2167203/150‑1 (MQ=60)
                                                                                     CACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAG                                                                >  SRR19973065.2078854/1‑150 (MQ=60)
                                                                                       CCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTG                                                              <  SRR19973065.1166359/150‑1 (MQ=60)
                                                                                        CAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGA                                                             >  SRR19973065.2664458/1‑150 (MQ=60)
                                                                                         AGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGAC                                                            <  SRR19973065.371533/150‑1 (MQ=60)
                                                                                             CCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCT                                                        >  SRR19973065.1678635/1‑150 (MQ=60)
                                                                                              CTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTC                                                       >  SRR19973065.1909167/1‑150 (MQ=60)
                                                                                              CTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTC                                                       >  SRR19973065.1908526/1‑150 (MQ=60)
                                                                                              CTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTC                                                       >  SRR19973065.1701161/1‑150 (MQ=60)
                                                                                                      CAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAA                                               >  SRR19973065.581380/1‑150 (MQ=60)
                                                                                                      CAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAA                                               >  SRR19973065.3284768/1‑150 (MQ=60)
                                                                                                         AAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGAT                                            <  SRR19973065.2105347/150‑1 (MQ=60)
                                                                                                         AAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCACATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGAT                                            >  SRR19973065.3030767/1‑150 (MQ=60)
                                                                                                             GGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTT                                        <  SRR19973065.1866522/150‑1 (MQ=60)
                                                                                                              GTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTT                                       <  SRR19973065.2724001/150‑1 (MQ=60)
                                                                                                              GTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTT                                       <  SRR19973065.255658/150‑1 (MQ=60)
                                                                                                                GTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGC                                     <  SRR19973065.2201413/150‑1 (MQ=60)
                                                                                                                GTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGC                                     <  SRR19973065.2201749/150‑1 (MQ=60)
                                                                                                                  ACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCA                                   >  SRR19973065.2107482/1‑150 (MQ=60)
                                                                                                                     GGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCT                                <  SRR19973065.1000940/150‑1 (MQ=60)
                                                                                                                      GGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTG                               <  SRR19973065.2054523/150‑1 (MQ=60)
                                                                                                                       GTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGT                              <  SRR19973065.2505228/150‑1 (MQ=60)
                                                                                                                       GTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGT                              <  SRR19973065.147434/150‑1 (MQ=60)
                                                                                                                       GTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGT                              <  SRR19973065.1637971/150‑1 (MQ=60)
                                                                                                                       GTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGT                              <  SRR19973065.1638944/150‑1 (MQ=60)
                                                                                                                        TTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTT                             <  SRR19973065.1698677/150‑1 (MQ=60)
                                                                                                                        TTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTT                             <  SRR19973065.3025425/150‑1 (MQ=60)
                                                                                                                         TGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTG                            <  SRR19973065.677617/150‑1 (MQ=60)
                                                                                                                          GAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGG                           <  SRR19973065.2933551/150‑1 (MQ=60)
                                                                                                                          GAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGG                           <  SRR19973065.2933760/150‑1 (MQ=60)
                                                                                                                           AGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGG                          >  SRR19973065.2570774/1‑150 (MQ=60)
                                                                                                                           AGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGG                          >  SRR19973065.803049/1‑150 (MQ=60)
                                                                                                                           AGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGG                          >  SRR19973065.2570766/1‑150 (MQ=60)
                                                                                                                           AGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATAAGTTTAGAGTCCAGCGACGCCAGCTTACCGTCCCCTTTGGTCGGTTTTAGTGACGCCTCAACTCCAAGATGTTTTGCCAGCTGTTGGG                          >  SRR19973065.2068638/1‑150 (MQ=60)
                                                                                                                            GAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGC                         >  SRR19973065.1788125/1‑150 (MQ=60)
                                                                                                                            GAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGC                         >  SRR19973065.2718154/1‑150 (MQ=60)
                                                                                                                               ATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAA                      >  SRR19973065.3370434/1‑150 (MQ=60)
                                                                                                                                TCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAAT                     <  SRR19973065.1631043/150‑1 (MQ=60)
                                                                                                                                 CGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATT                    >  SRR19973065.83653/1‑150 (MQ=60)
                                                                                                                                    ATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCA                 >  SRR19973065.704041/1‑150 (MQ=60)
                                                                                                                                      TTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACT               <  SRR19973065.3432169/150‑1 (MQ=60)
                                                                                                                                      TTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACT               >  SRR19973065.950912/1‑150 (MQ=60)
                                                                                                                                         TTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCACATTCCACTTCA            >  SRR19973065.2455813/1‑150 (MQ=60)
                                                                                                                                             TGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAAC        >  SRR19973065.3039694/1‑150 (MQ=60)
                                                                                                                                              GCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAACC       <  SRR19973065.371141/150‑1 (MQ=60)
                                                                                                                                                 CTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAACCGGT    <  SRR19973065.114285/150‑1 (MQ=60)
                                                                                                                                                   AATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAACCGGTTA  <  SRR19973065.1189714/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
CTTCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAACCGGTTA  >  CP009273/1993383‑1993679

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑