Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F78 I0 R1 626 241.3 10389380 77.7% 8072548 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,997,314 G→T 23.8% intergenic (‑227/‑39) fliC ← / → fliD flagellar filament structural protein (flagellin)/flagellar filament capping protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,997,3140GT23.8% 393.5 / 104.9 171intergenic (‑227/‑39)fliC/fliDflagellar filament structural protein (flagellin)/flagellar filament capping protein
Reads supporting (aligned to +/- strand):  ref base G (61/69);  new base T (25/16);  total (86/85)
Fisher's exact test for biased strand distribution p-value = 1.52e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 179 total aligned reads displayed.

ATCGTCTGTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAA  >  CP009273/1997166‑1997463
                                                                                                                                                    |                                                                                                                                                     
aTCGTCTGTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCgg                                                                                                                                                      <  1:2845398/150‑1 (MQ=255)
aTCGTCTGTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCgg                                                                                                                                                      <  2:210681/150‑1 (MQ=255)
  cGTCTGTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTa                                                                                                                                                    >  1:4381171/1‑150 (MQ=255)
   gTCTGTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTAt                                                                                                                                                   >  1:1789262/1‑150 (MQ=255)
    tCTGTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTAtt                                                                                                                                                  <  1:2732140/150‑1 (MQ=255)
     cTGTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTc                                                                                                                                                 <  2:3359865/150‑1 (MQ=255)
      tGTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCg                                                                                                                                                >  1:4168876/1‑150 (MQ=255)
       gTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTATTGTTAGTCGCCGAAATACTCTTTTCTATGCCCCTTATTCCCGCTATTAAAAAATAAAATTAAACGTAAACTTTGAGCAATTAAGACCGATAACCCATGAATTCGt                                                                                                                                               >  1:3255010/1‑150 (MQ=255)
        tCGTAAAACAACCTTTATAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGtt                                                                                                                                              <  2:5069716/150‑1 (MQ=255)
        tCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGtt                                                                                                                                              <  2:2593602/150‑1 (MQ=255)
        tCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGtt                                                                                                                                              <  2:2593660/150‑1 (MQ=255)
        tCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGtt                                                                                                                                              <  2:5070164/150‑1 (MQ=255)
            aaaaCAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTAc                                                                                                                                          <  1:4470540/150‑1 (MQ=255)
              aacaacCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACgt                                                                                                                                        >  1:393214/1‑150 (MQ=255)
                  acCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCg                                                                                                                                    <  1:1451185/150‑1 (MQ=255)
                   ccTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGa                                                                                                                                   <  1:5149510/150‑1 (MQ=255)
                   ccTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGa                                                                                                                                   >  1:3752518/1‑150 (MQ=255)
                    cTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGaa                                                                                                                                  <  2:2554009/150‑1 (MQ=255)
                    cTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGaa                                                                                                                                  >  1:3527027/1‑150 (MQ=255)
                     tttAGAATTTTTTTCAAAAACTTCCATTTTTTGTTAGTCGCCGAAATACTATTTTCTCTGCCCCTTATTCCCGCTTTTAAAAAAAACAATTCAACTTAAACTTTGCGCAATTCAGACAGATTACCCCGGTTTTCGTTTTACGTGTCGaaa                                                                                                                                 <  2:1292170/150‑1 (MQ=255)
                     tttAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGaaa                                                                                                                                 >  2:954595/1‑150 (MQ=255)
                        aGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGAt                                                                                                                              >  2:2815760/1‑150 (MQ=255)
                         gAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATa                                                                                                                             <  1:4871585/150‑1 (MQ=255)
                          aaTTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATaa                                                                                                                            <  1:5001445/150‑1 (MQ=255)
                          aaTTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATaa                                                                                                                            >  2:2937987/1‑150 (MQ=255)
                           aTTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATaaa                                                                                                                           <  1:3796325/150‑1 (MQ=255)
                              tttttCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAgg                                                                                                                        <  2:2315067/150‑1 (MQ=255)
                               ttttCAAAAAAAGGCATTTTTTGTTATTCGCCGAAATTATCTTTTCTCTTCCACTTTTTCCAGATATTAATAAATTCAATTAATCGTAAACTTTGCGCACTTCAGACCGATAACCCCGGTATTCGTTTTTCGTGTCGAAAGATcaattga                                                                                                                       <  2:1607911/150‑5 (MQ=255)
                                  tCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAAt                                                                                                                    <  2:2036024/150‑1 (MQ=255)
                                   cAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATc                                                                                                                   <  2:2392788/150‑1 (MQ=255)
                                      aaaCAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCa                                                                                                                >  2:680611/1‑150 (MQ=255)
                                        aCAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATg                                                                                                              >  1:1120684/1‑150 (MQ=255)
                                         cAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATgg                                                                                                             <  2:1132905/150‑1 (MQ=255)
                                         cAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATgg                                                                                                             >  2:711735/1‑150 (MQ=255)
                                            ccATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCaa                                                                                                          <  1:2240990/150‑1 (MQ=255)
                                             cATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAg                                                                                                         <  2:881429/150‑1 (MQ=255)
                                             cATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAg                                                                                                         >  1:1833162/1‑150 (MQ=255)
                                                   ttGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTc                                                                                                   >  2:5017105/1‑150 (MQ=255)
                                                     gTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCAt                                                                                                 <  1:3343134/150‑1 (MQ=255)
                                                     gTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCAt                                                                                                 <  1:738038/150‑1 (MQ=255)
                                                     gTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCAt                                                                                                 <  1:4702534/150‑1 (MQ=255)
                                                         gTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCt                                                                                             <  2:768743/150‑1 (MQ=255)
                                                            gCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTGACGTGTCGAAAGATAAAAGGAAATCGCATGGCACGTATTTCATCGCTggg                                                                                          >  1:2789794/1‑150 (MQ=255)
                                                             ccGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGa                                                                                         <  1:4446825/150‑1 (MQ=255)
                                                             ccGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGa                                                                                         >  1:1074995/1‑150 (MQ=255)
                                                             ccGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGa                                                                                         >  1:2657132/1‑150 (MQ=255)
                                                                     ctctTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTc                                                                                 <  1:701266/150‑1 (MQ=255)
                                                                       ctTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAg                                                                               >  2:2999500/1‑150 (MQ=255)
                                                                           tctcTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCt                                                                           >  2:710945/1‑150 (MQ=255)
                                                                           tctcTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCt                                                                           >  2:981510/1‑150 (MQ=255)
                                                                             tctGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTgg                                                                         >  1:5089447/1‑150 (MQ=255)
                                                                                  cccTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATATCATCGCTGGGAGTCGGGTCAGGTCTGGATTTa                                                                    >  1:4232672/1‑150 (MQ=255)
                                                                                   ccTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTaa                                                                   >  2:4548774/1‑150 (MQ=255)
                                                                                    cTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAg                                                                  >  2:904795/1‑150 (MQ=255)
                                                                                      tATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGtt                                                                <  2:3657467/150‑1 (MQ=255)
                                                                                       aTTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTc                                                               >  2:1935653/1‑150 (MQ=255)
                                                                                        ttCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTcc                                                              >  2:4267642/1‑150 (MQ=255)
                                                                                          cccGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCAt                                                            <  2:4887001/150‑1 (MQ=255)
                                                                                            cGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATcc                                                          >  1:2586448/1‑150 (MQ=255)
                                                                                              cTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCtt                                                        <  1:2357606/150‑1 (MQ=255)
                                                                                               tATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTg                                                       <  1:493100/150‑1 (MQ=255)
                                                                                                 ttAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGAt                                                     <  2:3790449/150‑1 (MQ=255)
                                                                                                 ttAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCACGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGAt                                                     >  2:5009990/1‑150 (MQ=255)
                                                                                                 ttAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGAt                                                     <  2:238889/150‑1 (MQ=255)
                                                                                                  tAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATa                                                    >  2:4642691/1‑150 (MQ=255)
                                                                                                  tAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATa                                                    <  1:2032953/150‑1 (MQ=255)
                                                                                                      aaaaaCAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCt                                                <  1:1628393/150‑1 (MQ=255)
                                                                                                       aaaaCAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTc                                               <  2:2688766/150‑1 (MQ=255)
                                                                                                       aaaaCAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTc                                               <  2:3846172/150‑1 (MQ=255)
                                                                                                        aaaCAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCa                                              <  1:2158441/150‑1 (MQ=255)
                                                                                                         aaCAATTAAACGTAAACTTTGCGCAAGTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCAc                                             <  2:3477483/150‑1 (MQ=255)
                                                                                                          aCAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCAcc                                            <  2:3208705/150‑1 (MQ=255)
                                                                                                          aCAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCAcc                                            <  2:413330/150‑1 (MQ=255)
                                                                                                           cAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCAccg                                           <  2:1749498/150‑1 (MQ=255)
                                                                                                           cAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCAccg                                           <  1:5021866/150‑1 (MQ=255)
                                                                                                               tAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACcgccgc                                       >  2:1928849/1‑150 (MQ=255)
                                                                                                               tAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACcgccgc                                       >  2:1929196/1‑150 (MQ=255)
                                                                                                                       aaCTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAg                               >  1:1925126/1‑150 (MQ=255)
                                                                                                                         cTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCg                             >  2:4279604/1‑150 (MQ=255)
                                                                                                                         cTTTGCGCAAGTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCg                             <  1:965916/150‑1 (MQ=255)
                                                                                                                            tGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGCTTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACg                          >  2:3642722/1‑150 (MQ=255)
                                                                                                                              cgcAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGcg                        >  1:602354/1‑149 (MQ=255)
                                                                                                                                cAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTaa                      >  2:4595058/1‑150 (MQ=255)
                                                                                                                                 aaTTCCGCCCGATAACCCGTGTTTGGGTTTTCCGTGTCTAAATATAAAAGCAAATCGCTTGGAATTTTTTTCATCGCTGGGTGTGGGGTCTGGTCTGTTTTTATGTTCCATCCTTGATAGCCTCACCGCCGCGAAAAAAGCGACGCTAAc                     <  1:3511861/150‑1 (MQ=255)
                                                                                                                                 aaTTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAAc                     >  1:3742522/1‑150 (MQ=255)
                                                                                                                                  aTTCAGCCCGATAACCCCGTTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCTTGGCAAGTATTTCATCGCTGGGAGTCGGTTCAGGTCTGTATTTAAGTTCCATCCTTTATAGCCTCACCGCCGCGCAAAAAGCGACGCTAAcc                    <  2:4381171/150‑1 (MQ=255)
                                                                                                                                   ttCAGACCGATAACCCCGGTATACGTTTTACGTGACGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAAccc                   >  2:1438195/1‑150 (MQ=255)
                                                                                                                                    tCAGACCGATAACCCCTTTATTCTTTTTACGTGTCGAAATATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAAcccc                  <  2:4168876/150‑1 (MQ=255)
                                                                                                                                    tCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAAcccc                  >  1:2707740/1‑150 (MQ=255)
                                                                                                                                     cAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAAccccc                 >  1:1520476/1‑150 (MQ=255)
                                                                                                                                       gACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCAt               >  1:4157096/1‑150 (MQ=255)
                                                                                                                                       gACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCAt               >  1:1562307/1‑150 (MQ=255)
                                                                                                                                         cctaTAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGTAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCTGGTCTGGATTTATGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCAttt             <  2:3858448/147‑1 (MQ=255)
                                                                                                                                         ccGATAACCCGGGTATTCGTTTTCCGTTTCGAAAGATAAATGGAAATCGCTTGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGTATTTAAGTTCCATCCTTGATAGCCTCCCCGCCGCGCAAAAAGCGACGCTAACCCCCAttt             <  2:2405498/150‑1 (MQ=255)
                                                                                                                                           gATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCa           >  1:1729303/1‑150 (MQ=255)
                                                                                                                                              aaCCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCAATTCAAAt        >  2:3078520/1‑150 (MQ=255)
                                                                                                                                                ccccTGTATTCTTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGAAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCa      <  2:5065791/150‑1 (MQ=255)
                                                                                                                                                ccccGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCa      >  1:3896200/1‑150 (MQ=255)
                                                                                                                                                  ccGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGc    <  2:4668975/150‑1 (MQ=255)
                                                                                                                                                    ggTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCaa  >  2:4262784/1‑150 (MQ=255)
                                                                                                                                                    |                                                                                                                                                     
ATCGTCTGTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCAA  >  CP009273/1997166‑1997463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A