Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F247 I0 R1 1496 299.8 9582018 97.8% 9371213 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,997,314 G→T 100% intergenic (‑227/‑39) fliC ← / → fliD flagellar filament structural protein (flagellin)/flagellar filament capping protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,997,3140GT99.4% 1010.7 / ‑3.6 302intergenic (‑227/‑39)fliC/fliDflagellar filament structural protein (flagellin)/flagellar filament capping protein
Reads supporting (aligned to +/- strand):  ref base G (1/2);  new base T (161/137);  total (163/139)
Fisher's exact test for biased strand distribution p-value = 5.97e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 302 total aligned reads displayed.

TCTGTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCA  >  CP009273/1997170‑1997459
                                                                                                                                                |                                                                                                                                                 
 cTGTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTAtt                                                                                                                                              >  1:4598030/1‑149 (MQ=255)
  tGTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTc                                                                                                                                             <  2:610987/149‑1 (MQ=255)
    tCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGt                                                                                                                                           >  1:3382634/1‑149 (MQ=255)
    tCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGt                                                                                                                                           >  2:184695/1‑149 (MQ=255)
      gTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGttt                                                                                                                                         >  1:2597207/1‑149 (MQ=255)
          aacaacCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATACGTTTTACg                                                                                                                                     >  2:2958431/1‑149 (MQ=255)
           acaacCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACgt                                                                                                                                    >  1:4395652/1‑149 (MQ=255)
            caacCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACgtg                                                                                                                                   <  2:1773363/149‑1 (MQ=255)
             aacCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACgtgt                                                                                                                                  >  1:3785927/1‑149 (MQ=255)
             aacCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACgtgt                                                                                                                                  >  2:1778344/1‑149 (MQ=255)
               ccTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCg                                                                                                                                >  2:4436828/1‑149 (MQ=255)
                cTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGa                                                                                                                               >  2:1598602/1‑149 (MQ=255)
                    aGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGa                                                                                                                           <  1:451479/149‑1 (MQ=255)
                      aaTTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATa                                                                                                                         <  2:4752090/149‑1 (MQ=255)
                      aaTTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATa                                                                                                                         <  2:3368352/149‑1 (MQ=255)
                       aTTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATaa                                                                                                                        <  2:2234581/149‑1 (MQ=255)
                          tttttCAAAAACAGCCATTTTTTGTTA‑‑‑GCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGaa                                                                                                                  <  2:1839974/149‑1 (MQ=255)
                           ttttCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAgg                                                                                                                    <  2:3222363/149‑1 (MQ=255)
                            tttCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGa                                                                                                                   >  2:46344/1‑149 (MQ=255)
                             ttCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGaa                                                                                                                  <  1:1987206/149‑1 (MQ=255)
                              tCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGaaa                                                                                                                 <  1:1210525/149‑1 (MQ=255)
                                 aaaaCAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCg                                                                                                              >  2:2264590/1‑149 (MQ=255)
                                   aaCAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCa                                                                                                            >  1:4679998/1‑149 (MQ=255)
                                    aCAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCAt                                                                                                           >  2:725643/1‑149 (MQ=255)
                                        ccATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTAGTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCa                                                                                                       <  2:67403/149‑1 (MQ=255)
                                         cATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCaa                                                                                                      >  1:18225/1‑149 (MQ=255)
                                         cATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCaa                                                                                                      <  2:47837/149‑1 (MQ=255)
                                         cATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCaa                                                                                                      <  2:4142017/149‑1 (MQ=255)
                                         cATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCaa                                                                                                      >  1:2089117/1‑149 (MQ=255)
                                         cATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCaa                                                                                                      <  1:1917281/149‑1 (MQ=255)
                                           ttttttGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGt                                                                                                    >  2:3086632/1‑149 (MQ=255)
                                            tttttGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTa                                                                                                   >  1:1685890/1‑149 (MQ=255)
                                              tttGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTAtt                                                                                                 <  2:2587901/149‑1 (MQ=255)
                                               ttGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTAttt                                                                                                <  1:4290566/149‑1 (MQ=255)
                                                tGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTc                                                                                               <  2:2826518/149‑1 (MQ=255)
                                                 gTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCa                                                                                              <  1:2302682/149‑1 (MQ=255)
                                                  ttAGTCGCCTTAATACTCTTTTCTCTGCCCATTATTCCCTCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCACTGTATTCGTTTTACGTGTCGAAAGTTAAAAGGAAATCGCATGGCAAGTATTTCAt                                                                                             <  1:4274397/149‑1 (MQ=255)
                                                  ttAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCAt                                                                                             >  1:2558823/1‑149 (MQ=255)
                                                    aGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGAGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCg                                                                                           >  2:4252440/1‑149 (MQ=255)
                                                     gTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGc                                                                                          >  2:4081274/1‑149 (MQ=255)
                                                     gTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGc                                                                                          >  1:4787029/1‑149 (MQ=255)
                                                           gAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAg                                                                                    >  1:3719645/1‑149 (MQ=255)
                                                            aaaTACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGt                                                                                   <  1:4669944/149‑1 (MQ=255)
                                                            aaaTACTCTTTTCTCTGCCCCTTAGTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGt                                                                                   <  1:360469/149‑1 (MQ=255)
                                                             aaTACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCGTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTc                                                                                  >  1:3165118/1‑149 (MQ=255)
                                                              aTACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCg                                                                                 >  2:842748/1‑149 (MQ=255)
                                                                aCTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCggg                                                                               <  2:3217829/149‑1 (MQ=255)
                                                                aCTCTTTTCTCTGCCCCTTATTCCCGATATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCggg                                                                               <  1:1527198/149‑1 (MQ=255)
                                                                aCTCTTTTCTCTGCCCCTTAGTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCggg                                                                               <  1:4467748/149‑1 (MQ=255)
                                                                  tctTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTc                                                                             >  2:1021917/1‑149 (MQ=255)
                                                                   ctTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCa                                                                            >  1:1003627/1‑149 (MQ=255)
                                                                       tctcTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTc                                                                        <  1:1611670/149‑1 (MQ=255)
                                                                         tctGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTg                                                                      >  2:2645345/1‑149 (MQ=255)
                                                                           tGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGGACACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGa                                                                    <  1:3993560/149‑1 (MQ=255)
                                                                             ccccTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGAtt                                                                  <  1:2739121/149‑1 (MQ=255)
                                                                                 ttATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAg                                                              >  2:2209599/1‑149 (MQ=255)
                                                                                  tATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGt                                                             <  2:1744198/149‑1 (MQ=255)
                                                                                  tATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGt                                                             <  2:2513135/149‑1 (MQ=255)
                                                                                    ttCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTc                                                           >  2:291095/1‑149 (MQ=255)
                                                                                    ttCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTc                                                           <  1:2050200/149‑1 (MQ=255)
                                                                                      cccGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCa                                                         >  2:1481250/1‑149 (MQ=255)
                                                                                      cccGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCa                                                         >  1:4751256/1‑149 (MQ=255)
                                                                                        cGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATc                                                       <  2:3476895/149‑1 (MQ=255)
                                                                                         gCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAAGTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATcc                                                      <  1:811336/149‑1 (MQ=255)
                                                                                          cTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCt                                                     <  2:2793230/149‑1 (MQ=255)
                                                                                             ttAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGa                                                  <  2:3864535/149‑1 (MQ=255)
                                                                                             ttAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGa                                                  >  2:471376/1‑149 (MQ=255)
                                                                                              tAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGAt                                                 >  2:4575091/1‑149 (MQ=255)
                                                                                               aaaaaaaaCAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATa                                                >  1:3053247/1‑149 (MQ=255)
                                                                                                  aaaaaCAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGcc                                             >  1:2220748/1‑149 (MQ=255)
                                                                                                    aaaCAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTc                                           >  1:3513312/1‑149 (MQ=255)
                                                                                                    aaaCAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTc                                           >  1:4078982/1‑149 (MQ=255)
                                                                                                     aaCAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCa                                          >  1:2890969/1‑149 (MQ=255)
                                                                                                       cAATTAAACGTCAACTTTGCGCAATTCAGACCGATAACCCCTGTAGTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCAcc                                        <  2:958199/149‑1 (MQ=255)
                                                                                                         aTTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCAccgc                                      >  2:1672533/1‑149 (MQ=255)
                                                                                                          ttAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCAccgcc                                     >  2:2145284/1‑149 (MQ=255)
                                                                                                             aaCGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCcgcg                                  >  1:75827/1‑149 (MQ=255)
                                                                                                             aaCGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCcgcg                                  >  2:486403/1‑149 (MQ=255)
                                                                                                             aaCGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCcgcg                                  >  1:294060/1‑149 (MQ=255)
                                                                                                              aCGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCgcgc                                 <  2:1542823/149‑1 (MQ=255)
                                                                                                              aCGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCgcgc                                 >  2:1323664/1‑149 (MQ=255)
                                                                                                                     cTTTGCGCAATTCAGCCCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGc                          <  1:951394/149‑1 (MQ=255)
                                                                                                                      tttGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCg                         <  2:2351926/149‑1 (MQ=255)
                                                                                                                      tttGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCg                         >  2:264780/1‑149 (MQ=255)
                                                                                                                      tttGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCg                         >  2:1626838/1‑149 (MQ=255)
                                                                                                                        tGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGTAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGAc                       <  1:627015/149‑1 (MQ=255)
                                                                                                                         gcgcAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACg                      <  2:2841577/149‑1 (MQ=255)
                                                                                                                             aaTTCAGACCGATAACCCCTGTTTTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGATGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTaa                  <  2:1146279/149‑1 (MQ=255)
                                                                                                                                tCAGACCGATAACCCCTGTATTCGTTTTACGTTTCGAAATATAAAAGTAAATCGCATGGAATGTATTTCTTCGCTGGGAGTCGGGTCAGTTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAAccc               <  2:4285842/149‑1 (MQ=255)
                                                                                                                                 cAGACCGAAAACCCCTGTATTCTTTTTACGTGTCTAAATATAAAAGGAAATCGCATGGCATGTATTTCATCGCTGGGAGTCGGGTCTGGTCTGTATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGAAAAAAGCGACGCTAAcccc              <  2:1288645/149‑1 (MQ=255)
                                                                                                                                  aGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAAccccc             >  1:3598618/1‑149 (MQ=255)
                                                                                                                                   gACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCa            >  1:1009690/1‑149 (MQ=255)
                                                                                                                                    aCCGATAACCCGTGTATTGTTTTTACGTGTCGAATGATAAAAGGAATTCGCATGGAAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGTTTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAACGCGACGCTAACCCCCAt           <  2:3134539/149‑1 (MQ=255)
                                                                                                                                    aCCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCAt           >  2:3732392/1‑149 (MQ=255)
                                                                                                                                    aCCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCAt           >  1:1427719/1‑149 (MQ=255)
                                                                                                                                     ccGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCAtt          >  2:3994324/1‑149 (MQ=255)
                                                                                                                                     ccGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCAtt          >  2:2098084/1‑149 (MQ=255)
                                                                                                                                           aCCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCACAAAGCGACGCTAACCCCCATTTCAAAt    >  2:3100880/1‑149 (MQ=255)
                                                                                                                                           aCCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAAt    >  2:710233/1‑149 (MQ=255)
                                                                                                                                             cccTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCa  >  1:1312769/1‑149 (MQ=255)
                                                                                                                                                |                                                                                                                                                 
TCTGTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCA  >  CP009273/1997170‑1997459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TATCGTCTGTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCA  >  CP009273/1997165‑1997459
                                                                                                                                                     |                                                                                                                                                 
TATCGTCTGTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCT                                                                                                                                                   <  SRR19973071.718941/150‑1 (MQ=60)
TATCGTCTGTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCT                                                                                                                                                   <  SRR19973071.4156856/150‑1 (MQ=60)
TATCGTCTGTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCT                                                                                                                                                   <  SRR19973071.1759677/150‑1 (MQ=60)
 ATCGTCTGTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTG                                                                                                                                                  >  SRR19973071.985559/1‑150 (MQ=60)
    GTCTGTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTAT                                                                                                                                               >  SRR19973071.944320/1‑150 (MQ=60)
    GTCTGTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTAT                                                                                                                                               >  SRR19973071.944269/1‑150 (MQ=60)
     TCTGTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATT                                                                                                                                              >  SRR19973071.4614421/1‑150 (MQ=60)
        GTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGT                                                                                                                                           >  SRR19973071.3394635/1‑150 (MQ=60)
        GTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGT                                                                                                                                           >  SRR19973071.3550771/1‑150 (MQ=60)
          CGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTT                                                                                                                                         >  SRR19973071.2606606/1‑150 (MQ=60)
           GTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTT                                                                                                                                        >  SRR19973071.1218540/1‑150 (MQ=60)
           GTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTT                                                                                                                                        >  SRR19973071.1218575/1‑150 (MQ=60)
               AACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGT                                                                                                                                    >  SRR19973071.3117356/1‑150 (MQ=60)
               AACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGT                                                                                                                                    >  SRR19973071.4411082/1‑150 (MQ=60)
               AACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGT                                                                                                                                    >  SRR19973071.1826015/1‑150 (MQ=60)
                 CAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGT                                                                                                                                  >  SRR19973071.3799381/1‑150 (MQ=60)
                 CAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGT                                                                                                                                  >  SRR19973071.3799394/1‑150 (MQ=60)
                  AACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTC                                                                                                                                 >  SRR19973071.2308695/1‑150 (MQ=60)
                  AACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTC                                                                                                                                 >  SRR19973071.4713622/1‑150 (MQ=60)
                    CCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGA                                                                                                                               >  SRR19973071.1435327/1‑150 (MQ=60)
                    CCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGA                                                                                                                               >  SRR19973071.4508830/1‑150 (MQ=60)
                     CTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAA                                                                                                                              <  SRR19973071.2892115/150‑1 (MQ=60)
                        TAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGA                                                                                                                           <  SRR19973071.1320858/150‑1 (MQ=60)
                         AGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGAT                                                                                                                          <  SRR19973071.453104/150‑1 (MQ=60)
                          GAAATTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATA                                                                                                                         >  SRR19973071.1557117/1‑150 (MQ=60)
                           AATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAA                                                                                                                        <  SRR19973071.1544276/150‑1 (MQ=60)
                                 TTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAA                                                                                                                  >  SRR19973071.3737533/1‑150 (MQ=60)
                                  TTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAA                                                                                                                 <  SRR19973071.1804754/150‑1 (MQ=60)
                                  TTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAA                                                                                                                 <  SRR19973071.1994382/150‑1 (MQ=60)
                                   TCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAAT                                                                                                                <  SRR19973071.1214693/150‑1 (MQ=60)
                                   TCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAAGTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAAT                                                                                                                <  SRR19973071.3611617/150‑1 (MQ=60)
                                       AAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCA                                                                                                            >  SRR19973071.4696643/1‑150 (MQ=60)
                                       AAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATCAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCA                                                                                                            >  SRR19973071.4696548/1‑150 (MQ=60)
                                          CAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGG                                                                                                         <  SRR19973071.4192009/150‑1 (MQ=60)
                                             CCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAA                                                                                                      >  SRR19973071.2669430/1‑150 (MQ=60)
                                             CCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAA                                                                                                      >  SRR19973071.4301011/1‑150 (MQ=60)
                                             CCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAA                                                                                                      >  SRR19973071.2096812/1‑150 (MQ=60)
                                             CCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAA                                                                                                      >  SRR19973071.18348/1‑150 (MQ=60)
                                             CCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAA                                                                                                      >  SRR19973071.4503580/1‑150 (MQ=60)
                                             CCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAA                                                                                                      >  SRR19973071.2542212/1‑150 (MQ=60)
                                              CATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAG                                                                                                     <  SRR19973071.1924294/150‑1 (MQ=60)
                                              CATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAG                                                                                                     <  SRR19973071.1406286/150‑1 (MQ=60)
                                              CATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAG                                                                                                     >  SRR19973071.4630365/1‑150 (MQ=60)
                                                TTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTA                                                                                                   >  SRR19973071.1692113/1‑150 (MQ=60)
                                                 TTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTAT                                                                                                  <  SRR19973071.3969508/150‑1 (MQ=60)
                                                   TTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTT                                                                                                <  SRR19973071.666387/150‑1 (MQ=60)
                                                    TTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTC                                                                                               <  SRR19973071.4305754/150‑1 (MQ=60)
                                                     TGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCACGTATTTCA                                                                                              >  SRR19973071.3645806/1‑150 (MQ=60)
                                                     TGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCA                                                                                              <  SRR19973071.4204696/150‑1 (MQ=60)
                                                     TGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCA                                                                                              >  SRR19973071.4090172/1‑150 (MQ=60)
                                                      GTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCAT                                                                                             <  SRR19973071.2311101/150‑1 (MQ=60)
                                                      GTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCAT                                                                                             >  SRR19973071.2568114/1‑150 (MQ=60)
                                                       TTAGTCGCCTTAATACTCTTTTCTCTGCCCATTATTCCCTCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCACTGTATTCGTTTTACGTGTCGAAAGTTAAAAGGAAATCGCATGGCAAGTATTTCATC                                                                                            <  SRR19973071.4289542/150‑1 (MQ=60)
                                                       TTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTGTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATC                                                                                            <  SRR19973071.4289571/150‑1 (MQ=60)
                                                         AGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGC                                                                                          >  SRR19973071.4803922/1‑150 (MQ=60)
                                                         AGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGC                                                                                          >  SRR19973071.4803898/1‑150 (MQ=60)
                                                             GCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGG                                                                                      >  SRR19973071.1464904/1‑150 (MQ=60)
                                                               CGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAG                                                                                    >  SRR19973071.648427/1‑150 (MQ=60)
                                                               CGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAG                                                                                    >  SRR19973071.3732826/1‑150 (MQ=60)
                                                               CGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAG                                                                                    >  SRR19973071.3047955/1‑150 (MQ=60)
                                                                 AAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCGTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTC                                                                                  >  SRR19973071.3176170/1‑150 (MQ=60)
                                                                 AAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCGTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTC                                                                                  >  SRR19973071.3176189/1‑150 (MQ=60)
                                                                 AAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTC                                                                                  <  SRR19973071.4686570/150‑1 (MQ=60)
                                                                 AAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTC                                                                                  <  SRR19973071.4686231/150‑1 (MQ=60)
                                                                 AAATACTCTTTTCTCTGCCCCTTAGTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTC                                                                                  <  SRR19973071.361631/150‑1 (MQ=60)
                                                                 AAATACTCTTGTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTC                                                                                  <  SRR19973071.4686708/150‑1 (MQ=60)
                                                                  AATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAAGTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCG                                                                                 <  SRR19973071.4469237/150‑1 (MQ=60)
                                                                  AATACTCTTTTCTCTGCCCCTTAGTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCG                                                                                 <  SRR19973071.4469224/150‑1 (MQ=60)
                                                                     ACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGT                                                                              <  SRR19973071.2077437/150‑1 (MQ=60)
                                                                     ACTCTTTTCTCTGCCCCTTATTCCCGATATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGT                                                                              <  SRR19973071.2204190/150‑1 (MQ=60)
                                                                     ACTCTTTTCTCTGCCCCTTATTCCCGATATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGT                                                                              <  SRR19973071.1532696/150‑1 (MQ=60)
                                                                     ACTCTTTTCTCTGCCCCTTAGTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGT                                                                              <  SRR19973071.4483585/150‑1 (MQ=60)
                                                                       TCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCA                                                                            >  SRR19973071.1007275/1‑150 (MQ=60)
                                                                            TCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCT                                                                       <  SRR19973071.1617379/150‑1 (MQ=60)
                                                                            TCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCT                                                                       <  SRR19973071.61736/150‑1 (MQ=60)
                                                                            TCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCT                                                                       <  SRR19973071.1065569/150‑1 (MQ=60)
                                                                            TCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAAGTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCT                                                                       <  SRR19973071.1617445/150‑1 (MQ=60)
                                                                                TGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGGACACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGAT                                                                   <  SRR19973071.4007515/150‑1 (MQ=60)
                                                                                 GCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATT                                                                  >  SRR19973071.4713180/1‑150 (MQ=60)
                                                                                  CCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTT                                                                 <  SRR19973071.2748514/150‑1 (MQ=60)
                                                                                  CCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTT                                                                 <  SRR19973071.1120565/150‑1 (MQ=60)
                                                                                  CCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTT                                                                 <  SRR19973071.3020558/150‑1 (MQ=60)
                                                                                  CCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTT                                                                 <  SRR19973071.3746923/150‑1 (MQ=60)
                                                                                  CCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTT                                                                 <  SRR19973071.2748838/150‑1 (MQ=60)
                                                                                    CCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAA                                                               >  SRR19973071.430951/1‑150 (MQ=60)
                                                                                         TTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCC                                                          <  SRR19973071.2057724/150‑1 (MQ=60)
                                                                                         TTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCC                                                          <  SRR19973071.2057855/150‑1 (MQ=60)
                                                                                          TCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCA                                                         >  SRR19973071.1985808/1‑150 (MQ=60)
                                                                                          TCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCA                                                         >  SRR19973071.4768063/1‑150 (MQ=60)
                                                                                             CGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCC                                                      >  SRR19973071.770934/1‑150 (MQ=60)
                                                                                              GCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAACTCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCT                                                     <  SRR19973071.4070540/150‑1 (MQ=60)
                                                                                              GCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCT                                                     <  SRR19973071.2763831/150‑1 (MQ=60)
                                                                                              GCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAAGTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCT                                                     <  SRR19973071.814079/150‑1 (MQ=60)
                                                                                                TATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTG                                                   >  SRR19973071.1009535/1‑150 (MQ=60)
                                                                                                  TTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGAT                                                 <  SRR19973071.1012924/150‑1 (MQ=60)
                                                                                                   TAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATA                                                >  SRR19973071.3064086/1‑150 (MQ=60)
                                                                                                      AAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCC                                             >  SRR19973071.2228947/1‑150 (MQ=60)
                                                                                                      AAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCC                                             >  SRR19973071.2228896/1‑150 (MQ=60)
                                                                                                       AAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCT                                            <  SRR19973071.4605746/150‑1 (MQ=60)
                                                                                                        AAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTC                                           >  SRR19973071.3525641/1‑150 (MQ=60)
                                                                                                        AAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTC                                           >  SRR19973071.4093453/1‑150 (MQ=60)
                                                                                                        AAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTC                                           >  SRR19973071.3525862/1‑150 (MQ=60)
                                                                                                         AAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCA                                          >  SRR19973071.786855/1‑150 (MQ=60)
                                                                                                         AAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCA                                          >  SRR19973071.2901312/1‑150 (MQ=60)
                                                                                                         AAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCA                                          >  SRR19973071.2916037/1‑150 (MQ=60)
                                                                                                           ACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACC                                        <  SRR19973071.1997729/150‑1 (MQ=60)
                                                                                                           ACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACC                                        <  SRR19973071.1997719/150‑1 (MQ=60)
                                                                                                                TAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGC                                   >  SRR19973071.3277159/1‑150 (MQ=60)
                                                                                                                 AAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCG                                  >  SRR19973071.294985/1‑150 (MQ=60)
                                                                                                                 AAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCG                                  >  SRR19973071.295113/1‑150 (MQ=60)
                                                                                                                 AAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCG                                  >  SRR19973071.76221/1‑150 (MQ=60)
                                                                                                                   ACGTAAACTTTGCGCAAGTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCA                                <  SRR19973071.1831739/150‑1 (MQ=60)
                                                                                                                     GTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAA                              >  SRR19973071.4793157/1‑150 (MQ=60)
                                                                                                                     GTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAA                              >  SRR19973071.4793202/1‑150 (MQ=60)
                                                                                                                        AACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAG                           <  SRR19973071.1966280/150‑1 (MQ=60)
                                                                                                                          CTTTGCGCAATTCAGCCCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCG                         <  SRR19973071.954841/150‑1 (MQ=60)
                                                                                                                          CTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCG                         >  SRR19973071.3149152/1‑150 (MQ=60)
                                                                                                                          CTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCG                         >  SRR19973071.4316599/1‑150 (MQ=60)
                                                                                                                           TTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGA                        <  SRR19973071.3706031/150‑1 (MQ=60)
                                                                                                                            TTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGAC                       <  SRR19973071.2289436/150‑1 (MQ=60)
                                                                                                                            TTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGAC                       <  SRR19973071.4553217/150‑1 (MQ=60)
                                                                                                                            TTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGAC                       <  SRR19973071.1688810/150‑1 (MQ=60)
                                                                                                                             TGCGCAATTCCGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACG                      <  SRR19973071.2583520/150‑1 (MQ=60)
                                                                                                                             TGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGTAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACG                      <  SRR19973071.629320/150‑1 (MQ=60)
                                                                                                                             TGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACG                      <  SRR19973071.3516624/150‑1 (MQ=60)
                                                                                                                             TGCGAAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGTATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACG                      <  SRR19973071.2583957/150‑1 (MQ=60)
                                                                                                                                      CAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCC             >  SRR19973071.352372/1‑150 (MQ=60)
                                                                                                                                      CAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCC             >  SRR19973071.3611136/1‑150 (MQ=60)
                                                                                                                                      CAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCC             >  SRR19973071.3611157/1‑150 (MQ=60)
                                                                                                                                       AGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCA            >  SRR19973071.2519354/1‑150 (MQ=60)
                                                                                                                                       AGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCA            >  SRR19973071.1013359/1‑150 (MQ=60)
                                                                                                                                        GACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCAT           >  SRR19973071.1432967/1‑150 (MQ=60)
                                                                                                                                           CGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTC        >  SRR19973071.1922512/1‑150 (MQ=60)
                                                                                                                                                 CCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCA  >  SRR19973071.1317583/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                 
TATCGTCTGTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCA  >  CP009273/1997165‑1997459

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑