Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,997,314 G→T 92.3% intergenic (‑227/‑39) fliC ← / → fliD flagellar filament structural protein (flagellin)/flagellar filament capping protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,997,3140GT92.3% 668.2 / 44.5 221intergenic (‑227/‑39)fliC/fliDflagellar filament structural protein (flagellin)/flagellar filament capping protein
Reads supporting (aligned to +/- strand):  ref base G (9/8);  new base T (113/91);  total (122/99)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.04e-01

Only 100 of 221 total aligned reads displayed.

CGTCTGTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCA  >  CP009273/1997168‑1997462
                                                                                                                                                  |                                                                                                                                                    
cGTCTGTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTa                                                                                                                                                   >  1:1299784/1‑150 (MQ=255)
cGTCTGTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTa                                                                                                                                                   <  1:3411708/150‑1 (MQ=255)
 gTCTGTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTAt                                                                                                                                                  >  1:633781/1‑150 (MQ=255)
  tCTGTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTAtt                                                                                                                                                 <  1:3043198/150‑1 (MQ=255)
    tGTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCg                                                                                                                                               >  2:382640/1‑150 (MQ=255)
       cttAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTAGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGATATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGttt                                                                                                                                            <  1:2274109/148‑1 (MQ=255)
         tAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTa                                                                                                                                          <  2:1662634/150‑1 (MQ=255)
           aaaCAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACg                                                                                                                                        <  2:2698094/150‑1 (MQ=255)
            aacaacCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACgt                                                                                                                                       >  1:870580/1‑150 (MQ=255)
            aacaacCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACgt                                                                                                                                       >  2:4055258/1‑150 (MQ=255)
                acCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCg                                                                                                                                   >  2:4258482/1‑150 (MQ=255)
                 ccTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGa                                                                                                                                  <  1:3779611/150‑1 (MQ=255)
                  cTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGaa                                                                                                                                 >  2:3757425/1‑150 (MQ=255)
                   tttAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTAGTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGaaa                                                                                                                                <  2:1298679/150‑1 (MQ=255)
                     tAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGa                                                                                                                              <  1:3393785/150‑1 (MQ=255)
                       gAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATa                                                                                                                            >  1:3714914/1‑150 (MQ=255)
                       gAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATa                                                                                                                            >  1:2586475/1‑150 (MQ=255)
                        aaTTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATaa                                                                                                                           <  1:2234600/150‑1 (MQ=255)
                         aTTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATaaa                                                                                                                          >  1:3871923/1‑150 (MQ=255)
                              tttCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGaa                                                                                                                     <  1:1963410/150‑1 (MQ=255)
                              tttCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGaa                                                                                                                     >  2:1010915/1‑150 (MQ=255)
                              tttCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGaa                                                                                                                     <  2:1296956/150‑1 (MQ=255)
                               ttCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGaaa                                                                                                                    >  2:678408/1‑150 (MQ=255)
                                 cAAAAACAGCAATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTACACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATc                                                                                                                  <  2:2545896/150‑1 (MQ=255)
                                     aaCAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCAt                                                                                                              <  2:611633/150‑1 (MQ=255)
                                        aGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGc                                                                                                           >  1:1231009/1‑150 (MQ=255)
                                        aGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGc                                                                                                           >  1:672304/1‑150 (MQ=255)
                                          ccATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCACATGGCaa                                                                                                         <  2:194000/150‑1 (MQ=255)
                                           cATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAg                                                                                                        <  2:1040132/150‑1 (MQ=255)
                                            aTTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGt                                                                                                       <  2:4325827/150‑1 (MQ=255)
                                             ttttttGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTa                                                                                                      >  1:2346908/1‑150 (MQ=255)
                                               ttttGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAACCGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTAtt                                                                                                    <  2:3928547/150‑1 (MQ=255)
                                                tttGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTAttt                                                                                                   <  2:1857793/150‑1 (MQ=255)
                                                 ttGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTc                                                                                                  >  2:2439144/1‑150 (MQ=255)
                                                  tGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCa                                                                                                 <  1:345995/150‑1 (MQ=255)
                                                  tGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCa                                                                                                 <  1:2519790/150‑1 (MQ=255)
                                                   gTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCAt                                                                                                >  1:747753/1‑150 (MQ=255)
                                                   gTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCAt                                                                                                >  1:420665/1‑150 (MQ=255)
                                                     tAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCg                                                                                              <  1:2537988/150‑1 (MQ=255)
                                                     tAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCg                                                                                              <  1:2537207/150‑1 (MQ=255)
                                                       gTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCt                                                                                            >  2:2234304/1‑150 (MQ=255)
                                                         cNCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTgg                                                                                          >  1:973049/1‑150 (MQ=255)
                                                         cGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTgg                                                                                          >  1:1815348/1‑150 (MQ=255)
                                                          gCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTggg                                                                                         >  1:348444/1‑150 (MQ=255)
                                                             gAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGt                                                                                      >  2:4208692/1‑150 (MQ=255)
                                                                aTACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCgg                                                                                   <  1:1036404/150‑1 (MQ=255)
                                                                aTACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCgg                                                                                   >  1:3183561/1‑150 (MQ=255)
                                                                       tttCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGt                                                                            <  1:2417260/150‑1 (MQ=255)
                                                                       tttCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGt                                                                            <  2:510504/150‑1 (MQ=255)
                                                                       tttCTCTGCCCCTTAGTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTGTACGTGTCGACAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGt                                                                            <  2:1189436/150‑1 (MQ=255)
                                                                        ttCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTc                                                                           >  2:2348574/1‑150 (MQ=255)
                                                                          ctctGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTg                                                                         <  1:162099/150‑1 (MQ=255)
                                                                          ctctGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGAGAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTg                                                                         >  1:2677409/1‑150 (MQ=255)
                                                                            ctGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGa                                                                       >  2:1802132/1‑150 (MQ=255)
                                                                             tGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGAt                                                                      >  1:3099461/1‑150 (MQ=255)
                                                                             tGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACATAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGAt                                                                      >  1:1546965/1‑150 (MQ=255)
                                                                              gCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGAtt                                                                     <  2:3734843/150‑1 (MQ=255)
                                                                                   ttATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGt                                                                >  1:1706858/1‑150 (MQ=255)
                                                                                    tATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGtt                                                               >  1:1135192/1‑150 (MQ=255)
                                                                                        cccGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCAt                                                           <  2:2758024/150‑1 (MQ=255)
                                                                                          cGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATcc                                                         <  2:3541044/150‑1 (MQ=255)
                                                                                           gCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCt                                                        >  2:586637/1‑150 (MQ=255)
                                                                                            cTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCtt                                                       >  1:2928328/1‑150 (MQ=255)
                                                                                              aTTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGa                                                     >  2:2091083/1‑150 (MQ=255)
                                                                                               ttAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGAt                                                    >  2:1443963/1‑150 (MQ=255)
                                                                                                   aaaaaaCAATTAAACGTAAACTTTGCGCAAGTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGcc                                                <  2:2902400/150‑1 (MQ=255)
                                                                                                      aaaCAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCa                                             <  1:1900379/150‑1 (MQ=255)
                                                                                                      aaaCAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCa                                             <  1:4088526/150‑1 (MQ=255)
                                                                                                        aCAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCAcc                                           >  2:3038265/1‑150 (MQ=255)
                                                                                                           aTTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCAccgcc                                        >  1:2722788/1‑150 (MQ=255)
                                                                                                           aTTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCAccgcc                                        >  1:2821618/1‑150 (MQ=255)
                                                                                                               aaCGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCgcgc                                    <  2:73715/150‑1 (MQ=255)
                                                                                                               aaCGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCgcgc                                    >  2:122175/1‑150 (MQ=255)
                                                                                                                 cGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGAGTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCaa                                  >  2:3162997/1‑150 (MQ=255)
                                                                                                                    aaaCTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCaaaaa                               >  2:3283518/1‑150 (MQ=255)
                                                                                                                    aaaCTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCaaaaa                               >  2:3288237/1‑150 (MQ=255)
                                                                                                                    aaaCTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCaaaaa                               >  1:2282485/1‑150 (MQ=255)
                                                                                                                     aaCTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAg                              <  1:1103378/150‑1 (MQ=255)
                                                                                                                     aaCTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAg                              <  1:1917482/150‑1 (MQ=255)
                                                                                                                     aaCTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAg                              <  1:2762821/150‑1 (MQ=255)
                                                                                                                      aCTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGc                             <  1:119674/150‑1 (MQ=255)
                                                                                                                      aCTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGc                             >  2:1343692/1‑150 (MQ=255)
                                                                                                                      aCTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGc                             >  2:868027/1‑150 (MQ=255)
                                                                                                                      aCTTTGCGCAAGTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGc                             <  1:3918598/150‑1 (MQ=255)
                                                                                                                         ttGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGAc                          <  1:1320558/150‑1 (MQ=255)
                                                                                                                         ttGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGAc                          <  2:3458469/150‑1 (MQ=255)
                                                                                                                            cgcAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATGTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCt                       >  2:2404554/1‑150 (MQ=255)
                                                                                                                               aaTTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAAc                    >  2:1728827/1‑150 (MQ=255)
                                                                                                                               aaTTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAAc                    >  1:4028439/1‑150 (MQ=255)
                                                                                                                               aaTTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAAc                    >  1:3476034/1‑150 (MQ=255)
                                                                                                                               aaTTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGTGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCAGCGCAAAAAGCGACGCTAAc                    >  2:2036307/1‑150 (MQ=255)
                                                                                                                               aaTTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAAc                    >  2:3240189/1‑150 (MQ=255)
                                                                                                                                 ttCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAAccc                  >  2:1640868/1‑150 (MQ=255)
                                                                                                                                 ttCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAAccc                  >  2:202328/1‑150 (MQ=255)
                                                                                                                                  tCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAAcccc                 >  1:1379643/1‑150 (MQ=255)
                                                                                                                                      aCCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCAtt             >  1:3296880/1‑150 (MQ=255)
                                                                                                                                       ccGATAACCCCTGTTTTCTTTTTACGTGTCGAAAGATAAAAGGAAATCGCTTGGCAAGTATTTCATCGCTGGTAGTCGGGTCTGGTCTGTATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCAttt            <  2:2483233/150‑1 (MQ=255)
                                                                                                                                        cGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTc           >  2:2386177/1‑150 (MQ=255)
                                                                                                                                             aCCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATc      >  1:3350525/1‑150 (MQ=255)
                                                                                                                                                 cGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCa  >  2:3625094/1‑150 (MQ=255)
                                                                                                                                                  |                                                                                                                                                    
CGTCTGTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATCAGCA  >  CP009273/1997168‑1997462

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TATCGTCTGTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATC  >  CP009273/1997165‑1997458
                                                                                                                                                     |                                                                                                                                                
TATCGTCTGTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCT                                                                                                                                                  >  SRR19973079.2260511/1‑150 (MQ=60)
 ATCGTCTGTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTG                                                                                                                                                 >  SRR19973079.3559181/1‑150 (MQ=60)
   CGTCTGTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTA                                                                                                                                               >  SRR19973079.1307156/1‑150 (MQ=60)
   CGTCTGTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTA                                                                                                                                               <  SRR19973079.3429318/150‑1 (MQ=60)
   CGTCTGTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTA                                                                                                                                               <  SRR19973079.3429285/150‑1 (MQ=60)
    GTCTGTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTAT                                                                                                                                              >  SRR19973079.637406/1‑150 (MQ=60)
     TCTGTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATT                                                                                                                                             <  SRR19973079.3058954/150‑1 (MQ=60)
      CTGTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTC                                                                                                                                            <  SRR19973079.744018/150‑1 (MQ=60)
       TGTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCG                                                                                                                                           >  SRR19973079.2060050/1‑150 (MQ=60)
       TGTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCG                                                                                                                                           <  SRR19973079.3001925/150‑1 (MQ=60)
       TGTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCG                                                                                                                                           <  SRR19973079.426220/150‑1 (MQ=60)
          CTTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTAGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGATATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTT                                                                                                                                        <  SRR19973079.2285926/150‑1 (MQ=60)
          CGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTT                                                                                                                                        <  SRR19973079.1624684/150‑1 (MQ=60)
          CGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTT                                                                                                                                        >  SRR19973079.2328118/1‑150 (MQ=60)
          CGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTT                                                                                                                                        >  SRR19973079.2588289/1‑150 (MQ=60)
              AAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACG                                                                                                                                    <  SRR19973079.1088998/150‑1 (MQ=60)
               AACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGT                                                                                                                                   >  SRR19973079.875617/1‑150 (MQ=60)
                 CAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGT                                                                                                                                 >  SRR19973079.3476322/1‑150 (MQ=60)
                   ACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCG                                                                                                                               >  SRR19973079.319390/1‑150 (MQ=60)
                    CCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGA                                                                                                                              <  SRR19973079.3798973/150‑1 (MQ=60)
                        TAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGA                                                                                                                          <  SRR19973079.3411285/150‑1 (MQ=60)
                          GAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATA                                                                                                                        >  SRR19973079.3733918/1‑150 (MQ=60)
                          GAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATA                                                                                                                        <  SRR19973079.14236/150‑1 (MQ=60)
                          GAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATA                                                                                                                        >  SRR19973079.2599732/1‑150 (MQ=60)
                          GAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATA                                                                                                                        >  SRR19973079.2599745/1‑150 (MQ=60)
                          GAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATA                                                                                                                        <  SRR19973079.12372/150‑1 (MQ=60)
                           AATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAA                                                                                                                       <  SRR19973079.2246167/150‑1 (MQ=60)
                            ATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAA                                                                                                                      >  SRR19973079.3891619/1‑150 (MQ=60)
                                 TTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAA                                                                                                                 <  SRR19973079.1973584/150‑1 (MQ=60)
                                 TTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAA                                                                                                                 <  SRR19973079.1973854/150‑1 (MQ=60)
                                 TTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAAGTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAA                                                                                                                 <  SRR19973079.1974188/150‑1 (MQ=60)
                                          CAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGG                                                                                                        <  SRR19973079.3925262/150‑1 (MQ=60)
                                           AGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGC                                                                                                       >  SRR19973079.676027/1‑150 (MQ=60)
                                           AGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGC                                                                                                       >  SRR19973079.1238102/1‑150 (MQ=60)
                                           AGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGC                                                                                                       >  SRR19973079.1238081/1‑150 (MQ=60)
                                                TTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTA                                                                                                  >  SRR19973079.2359088/1‑150 (MQ=60)
                                                     TGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCA                                                                                             <  SRR19973079.347890/150‑1 (MQ=60)
                                                     TGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCA                                                                                             <  SRR19973079.2532529/150‑1 (MQ=60)
                                                     TGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCA                                                                                             <  SRR19973079.347970/150‑1 (MQ=60)
                                                      GTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCAT                                                                                            >  SRR19973079.423140/1‑150 (MQ=60)
                                                      GTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCAT                                                                                            >  SRR19973079.751811/1‑150 (MQ=60)
                                                       TTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATC                                                                                           <  SRR19973079.1680189/150‑1 (MQ=60)
                                                       TTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATC                                                                                           <  SRR19973079.4204340/150‑1 (MQ=60)
                                                        TAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCG                                                                                          <  SRR19973079.2550781/150‑1 (MQ=60)
                                                        TAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCG                                                                                          <  SRR19973079.2550038/150‑1 (MQ=60)
                                                        TAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCG                                                                                          <  SRR19973079.2549962/150‑1 (MQ=60)
                                                        TAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCG                                                                                          <  SRR19973079.2549998/150‑1 (MQ=60)
                                                            CNCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGG                                                                                      >  SRR19973079.978580/1‑150 (MQ=60)
                                                            CGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGG                                                                                      <  SRR19973079.2672134/150‑1 (MQ=60)
                                                            CGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGG                                                                                      >  SRR19973079.1824809/1‑150 (MQ=60)
                                                            CGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGG                                                                                      <  SRR19973079.3504802/150‑1 (MQ=60)
                                                             GCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGG                                                                                     <  SRR19973079.1339026/150‑1 (MQ=60)
                                                             GCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGG                                                                                     >  SRR19973079.350344/1‑150 (MQ=60)
                                                             GCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGG                                                                                     <  SRR19973079.1907183/150‑1 (MQ=60)
                                                                  AATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCG                                                                                >  SRR19973079.726813/1‑150 (MQ=60)
                                                                   ATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGG                                                                               <  SRR19973079.1042152/150‑1 (MQ=60)
                                                                   ATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGG                                                                               >  SRR19973079.3199837/1‑150 (MQ=60)
                                                                         TTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGG                                                                         >  SRR19973079.3054724/1‑150 (MQ=60)
                                                                          TTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGT                                                                        <  SRR19973079.2431080/150‑1 (MQ=60)
                                                                          TTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGT                                                                        <  SRR19973079.2429714/150‑1 (MQ=60)
                                                                            TCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCT                                                                      >  SRR19973079.2721430/1‑150 (MQ=60)
                                                                             CTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTG                                                                     <  SRR19973079.163344/150‑1 (MQ=60)
                                                                             CTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGAGAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTG                                                                     >  SRR19973079.2691288/1‑150 (MQ=60)
                                                                                TGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGAT                                                                  >  SRR19973079.3115458/1‑150 (MQ=60)
                                                                                TGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGAT                                                                  >  SRR19973079.3115468/1‑150 (MQ=60)
                                                                                TGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACATAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGAT                                                                  >  SRR19973079.1555508/1‑150 (MQ=60)
                                                                                 GCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATT                                                                 <  SRR19973079.1762277/150‑1 (MQ=60)
                                                                                      TTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGT                                                            >  SRR19973079.1715992/1‑150 (MQ=60)
                                                                                       TATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTT                                                           >  SRR19973079.1141695/1‑150 (MQ=60)
                                                                                       TATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTT                                                           >  SRR19973079.3721486/1‑150 (MQ=60)
                                                                                        ATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTC                                                          >  SRR19973079.2378274/1‑150 (MQ=60)
                                                                                           CCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCAT                                                       <  SRR19973079.324141/150‑1 (MQ=60)
                                                                                               CTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTT                                                   >  SRR19973079.2460728/1‑150 (MQ=60)
                                                                                               CTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTT                                                   >  SRR19973079.2943255/1‑150 (MQ=60)
                                                                                                TATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTG                                                  >  SRR19973079.4010806/1‑150 (MQ=60)
                                                                                                TATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTG                                                  >  SRR19973079.4010758/1‑150 (MQ=60)
                                                                                                   TAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATA                                               >  SRR19973079.3404189/1‑150 (MQ=60)
                                                                                                   TAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATA                                               <  SRR19973079.82221/150‑1 (MQ=60)
                                                                                                   TAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATA                                               >  SRR19973079.3404287/1‑150 (MQ=60)
                                                                                                   TAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGT                                                                                                                                 >  SRR19973079.431214/1‑68 (MQ=60)
                                                                                                      AAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCC                                            >  SRR19973079.643647/1‑150 (MQ=60)
                                                                                                       AAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCT                                           >  SRR19973079.2278179/1‑150 (MQ=60)
                                                                                                       AAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCT                                           >  SRR19973079.4073853/1‑150 (MQ=60)
                                                                                                         AAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCA                                         <  SRR19973079.4109246/150‑1 (MQ=60)
                                                                                                         AAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCA                                         <  SRR19973079.1911616/150‑1 (MQ=60)
                                                                                                         AAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCA                                         <  SRR19973079.1910497/150‑1 (MQ=60)
                                                                                                              ATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCC                                    >  SRR19973079.2736869/1‑150 (MQ=60)
                                                                                                              ATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCC                                    >  SRR19973079.2836043/1‑150 (MQ=60)
                                                                                                                       AAACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAA                           >  SRR19973079.2294337/1‑150 (MQ=60)
                                                                                                                        AACTTTGCGCACTTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAG                          <  SRR19973079.1109622/150‑1 (MQ=60)
                                                                                                                        AACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAG                          <  SRR19973079.2779529/150‑1 (MQ=60)
                                                                                                                        AACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAG                          <  SRR19973079.384801/150‑1 (MQ=60)
                                                                                                                        AACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAG                          <  SRR19973079.1927718/150‑1 (MQ=60)
                                                                                                                        AACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAG                          <  SRR19973079.2777042/150‑1 (MQ=60)
                                                                                                                        AACTTTGCGCAAGTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAG                          <  SRR19973079.1109563/150‑1 (MQ=60)
                                                                                                                         ACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGC                         <  SRR19973079.120675/150‑1 (MQ=60)
                                                                                                                         ACTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGC                         <  SRR19973079.120664/150‑1 (MQ=60)
                                                                                                                         ACTTTGCGCAAGTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGC                         <  SRR19973079.3938433/150‑1 (MQ=60)
                                                                                                                          CTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCG                        <  SRR19973079.3776676/150‑1 (MQ=60)
                                                                                                                          CTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCG                        >  SRR19973079.918422/1‑150 (MQ=60)
                                                                                                                          CTTTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCG                        >  SRR19973079.1292690/1‑150 (MQ=60)
                                                                                                                            TTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGAC                      <  SRR19973079.1328006/150‑1 (MQ=60)
                                                                                                                            TTGCGCAATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGAC                      <  SRR19973079.1328003/150‑1 (MQ=60)
                                                                                                                                  AATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAAC                >  SRR19973079.4048768/1‑150 (MQ=60)
                                                                                                                                  AATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAAC                >  SRR19973079.3493986/1‑150 (MQ=60)
                                                                                                                                  AATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAAC                >  SRR19973079.3493949/1‑150 (MQ=60)
                                                                                                                                  AATTCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAAC                >  SRR19973079.2749374/1‑150 (MQ=60)
                                                                                                                                     TCAGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCC             >  SRR19973079.1387273/1‑150 (MQ=60)
                                                                                                                                      CAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCC            >  SRR19973079.1630654/1‑150 (MQ=60)
                                                                                                                                      CAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCC            >  SRR19973079.333410/1‑150 (MQ=60)
                                                                                                                                       AGACCGATAACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCA           >  SRR19973079.2293027/1‑150 (MQ=60)
                                                                                                                                         ACCTATAACCCCTTTATTCTTTTTAGGTGTCTAAATATAAAAGTAAATCGCTTGGAAATTATTTAATGGCTGGGAGTGGGGTCAGTTCTGTATTTATGTTCCATCATTTATAGCCACACCGCCGCGAAAAAAGCGACGCTAACCCCCATT         <  SRR19973079.2890159/150‑1 (MQ=32)
                                                                                                                                         ACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATT         >  SRR19973079.3313661/1‑150 (MQ=60)
                                                                                                                                                ACCCCTGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATC  >  SRR19973079.3367725/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                
TATCGTCTGTCGTAAAACAACCTTTAGAATTTTTTTCAAAAACAGCCATTTTTTGTTAGTCGCCGAAATACTCTTTTCTCTGCCCCTTATTCCCGCTATTAAAAAAAACAATTAAACGTAAACTTTGCGCAATTCAGACCGATAACCCCGGTATTCGTTTTACGTGTCGAAAGATAAAAGGAAATCGCATGGCAAGTATTTCATCGCTGGGAGTCGGGTCAGGTCTGGATTTAAGTTCCATCCTTGATAGCCTCACCGCCGCGCAAAAAGCGACGCTAACCCCCATTTCAAATC  >  CP009273/1997165‑1997458

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑