Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,993,530 C→A 100% E113* (GAG→TAG)  fliY ← cystine transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,993,5300CA99.3% 795.8 / ‑3.8 243E113* (GAG→TAG) fliYcystine transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (127/114);  total (128/115)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.97e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 249 total aligned reads displayed.

CTTCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAACCG  >  CP009273/1993383‑1993675
                                                                                                                                                   |                                                                                                                                                 
ctTCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCtaat                                                                                                                                                 <  1:876855/150‑1 (MQ=255)
  tCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCtaatca                                                                                                                                               <  2:3116636/150‑1 (MQ=255)
   cATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCtaatcat                                                                                                                                              <  1:681363/150‑2 (MQ=255)
   cATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAg                                                                                                                                              >  1:3619444/1‑150 (MQ=255)
   cATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAg                                                                                                                                              <  1:32629/150‑1 (MQ=255)
    aTAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGa                                                                                                                                             >  2:4905254/1‑150 (MQ=255)
    aTAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTGCGTTACCTTTTTTCACCAGCGCCTGAAAACCAGAAATGGTGTACGGGGTTGAGAAATCGAATTTTTTCTTGCGCTAATCAGa                                                                                                                                             >  1:1548942/1‑150 (MQ=255)
       gTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAAt                                                                                                                                          <  1:122722/150‑1 (MQ=255)
         tGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATgg                                                                                                                                        >  1:1027433/1‑150 (MQ=255)
           gTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTg                                                                                                                                      <  2:3497175/150‑1 (MQ=255)
           gTGCCCAGACCGACCCCCACATTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCTTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTg                                                                                                                                      <  1:4342358/150‑1 (MQ=255)
            tGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGa                                                                                                                                     >  1:2223646/1‑150 (MQ=255)
                cAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTg                                                                                                                                 <  2:3594049/150‑1 (MQ=255)
                  gaccgaccCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGAt                                                                                                                               <  1:2534815/150‑1 (MQ=255)
                   accgaccCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGAtt                                                                                                                              >  2:2903898/1‑150 (MQ=255)
                    ccgaccCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTa                                                                                                                             <  1:3139725/150‑1 (MQ=255)
                    ccgaccCCCACTTTTTTGCCTTTCAGATCATCGGCTATTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTa                                                                                                                             >  2:5089446/1‑150 (MQ=255)
                         ccccACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATcac                                                                                                                        <  1:3499975/150‑1 (MQ=255)
                         ccccACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATcac                                                                                                                        >  2:1528423/1‑150 (MQ=255)
                           ccACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATcacca                                                                                                                      <  1:3182406/150‑1 (MQ=255)
                            cACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATcaccac                                                                                                                     <  1:4492896/150‑1 (MQ=255)
                            cACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATcaccac                                                                                                                     <  1:4297331/150‑1 (MQ=255)
                             aCTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACcaca                                                                                                                    >  1:4675881/1‑150 (MQ=255)
                               ttttttGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATc                                                                                                                  <  1:2414254/150‑1 (MQ=255)
                               ttttttGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATc                                                                                                                  <  2:2683638/150‑1 (MQ=255)
                                    tGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATAc                                                                                                             >  2:1962422/1‑150 (MQ=255)
                                          tCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTag                                                                                                       <  2:2732675/150‑1 (MQ=255)
                                           cAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTaga                                                                                                      <  1:238522/150‑1 (MQ=255)
                                                catcGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCa                                                                                                 <  2:3220648/150‑1 (MQ=255)
                                                catcGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCa                                                                                                 >  2:2361482/1‑150 (MQ=255)
                                                 atcGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAg                                                                                                <  2:3675216/150‑1 (MQ=255)
                                                 atcGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGATAATCAGAAATGGTGACCTTATTAATAACCACATCAATACGTTTAGAGTCCAg                                                                                                >  1:4899420/1‑150 (MQ=255)
                                                   cGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCg                                                                                              >  1:2510969/1‑150 (MQ=255)
                                                    ggCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGa                                                                                             <  2:566162/150‑1 (MQ=255)
                                                    ggCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGa                                                                                             >  1:4579395/1‑150 (MQ=255)
                                                    ggCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGa                                                                                             >  2:3238163/1‑150 (MQ=255)
                                                        gTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGcc                                                                                         <  1:3160399/150‑1 (MQ=255)
                                                         ttttAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCa                                                                                        <  1:764770/150‑1 (MQ=255)
                                                            tAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCa                                                                                     >  2:1340106/1‑150 (MQ=255)
                                                              aTGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATa                                                                                   >  1:547824/1‑150 (MQ=255)
                                                              aTGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATa                                                                                   >  1:2285804/1‑150 (MQ=255)
                                                               tGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATAc                                                                                  <  2:3057471/150‑1 (MQ=255)
                                                               tGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATAc                                                                                  >  2:4270241/1‑150 (MQ=255)
                                                                ggTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATAcc                                                                                 >  2:4785188/1‑150 (MQ=255)
                                                                   gCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTc                                                                              <  2:90265/150‑1 (MQ=255)
                                                                    ccTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTcc                                                                             >  1:5063767/1‑150 (MQ=255)
                                                                    ccTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTcc                                                                             >  2:608105/1‑150 (MQ=255)
                                                                     cTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTccc                                                                            >  2:1808442/1‑150 (MQ=255)
                                                                      ttCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCa                                                                           <  1:5071388/150‑1 (MQ=255)
                                                                         gTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGGTTAGAGTCCAGCGACGCCAGCATACCGTCCCAgtt                                                                        >  1:4851550/1‑150 (MQ=255)
                                                                           tACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTgg                                                                      >  2:3868445/1‑150 (MQ=255)
                                                                            aCCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGt                                                                     <  1:1793374/150‑1 (MQ=255)
                                                                            aCCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGt                                                                     >  1:2973848/1‑150 (MQ=255)
                                                                             ccTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTc                                                                    >  2:4034975/1‑150 (MQ=255)
                                                                             ccTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTc                                                                    <  2:1694899/150‑1 (MQ=255)
                                                                              cTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCg                                                                   >  2:413314/1‑150 (MQ=255)
                                                                               ttttttCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCgg                                                                  >  1:1574040/1‑150 (MQ=255)
                                                                                 ttttCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGtt                                                                <  2:3331236/150‑1 (MQ=255)
                                                                                 ttttCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGtt                                                                <  1:167659/150‑1 (MQ=255)
                                                                                 ttttCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGtt                                                                >  1:4225746/1‑150 (MQ=255)
                                                                                    tCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTa                                                             <  1:4741694/150‑1 (MQ=255)
                                                                                    tCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTa                                                             <  1:3345915/150‑1 (MQ=255)
                                                                                     cACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAg                                                            <  1:3952866/150‑1 (MQ=255)
                                                                                      aCCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGt                                                           <  2:4525430/150‑1 (MQ=255)
                                                                                        cAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGa                                                         >  1:1265075/1‑150 (MQ=255)
                                                                                        cAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGa                                                         <  2:11998/150‑1 (MQ=255)
                                                                                        cAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATGTGGTCGGTTTTAGTGa                                                         >  2:1978939/1‑150 (MQ=255)
                                                                                         aGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGAc                                                        <  2:3644523/150‑1 (MQ=255)
                                                                                         aGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCAGTTGGGCGGTTTTAGTGAc                                                        >  2:3384508/1‑150 (MQ=255)
                                                                                          gcgcCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACg                                                       <  1:1671764/150‑1 (MQ=255)
                                                                                           cgcCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTGAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGc                                                      >  2:1140570/1‑150 (MQ=255)
                                                                                             ccTGAATACCAGACATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCt                                                    <  1:3044594/150‑1 (MQ=255)
                                                                                             ccTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCt                                                    <  1:3989698/150‑1 (MQ=255)
                                                                                              cTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTc                                                   <  2:4369824/150‑1 (MQ=255)
                                                                                               tGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCa                                                  >  2:477002/1‑150 (MQ=255)
                                                                                               tGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCa                                                  >  1:1838876/1‑150 (MQ=255)
                                                                                                   tACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCATCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGc                                              >  2:3095496/1‑150 (MQ=255)
                                                                                                   tACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGc                                              >  2:1647237/1‑150 (MQ=255)
                                                                                                       aGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAg                                          >  2:1773565/1‑150 (MQ=255)
                                                                                                             ggTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGttt                                    <  2:5012983/150‑1 (MQ=255)
                                                                                                              gtgtACGGTGTTGAGAATTCGTATGTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCTTTACGTTTAGAGTCCAGCGACGCCTGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACTCCAAGATGtttt                                   <  2:3757522/150‑1 (MQ=255)
                                                                                                                gtACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTACTCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGc                                 <  2:3459914/150‑1 (MQ=255)
                                                                                                                gtACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGc                                 >  2:4575703/1‑150 (MQ=255)
                                                                                                                    ggggTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGc                             <  2:1027433/150‑1 (MQ=255)
                                                                                                                      ggTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTg                           <  1:4135089/150‑1 (MQ=255)
                                                                                                                      ggTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTg                           >  1:643503/1‑150 (MQ=255)
                                                                                                                          gagaAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTgg                       >  2:2569144/1‑150 (MQ=255)
                                                                                                                            gaAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGc                     <  1:3553935/150‑1 (MQ=255)
                                                                                                                              aaTCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCaa                   <  2:4247784/150‑1 (MQ=255)
                                                                                                                                  gTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTc               >  2:2525527/1‑150 (MQ=255)
                                                                                                                                    aTTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCa             >  2:761392/1‑150 (MQ=255)
                                                                                                                                      tttttCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACt           >  1:1736754/1‑150 (MQ=255)
                                                                                                                                       ttttCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACtt          <  1:2056816/150‑1 (MQ=255)
                                                                                                                                        tttCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTc         >  2:389608/1‑150 (MQ=255)
                                                                                                                                         ttCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCa        <  2:2200292/150‑1 (MQ=255)
                                                                                                                                            ttGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCaaaa     <  1:378411/150‑1 (MQ=255)
                                                                                                                                             tGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAAc    <  1:3092766/150‑1 (MQ=255)
                                                                                                                                               cgcTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCCGCTGTTGGGCACATTCCACTTCAAAACCg  >  2:4354688/1‑150 (MQ=255)
                                                                                                                                               cgcTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCCGCTGTTGGGCAACTTCCACTTCAAAACCg  >  2:1494331/1‑150 (MQ=255)
                                                                                                                                               cgcTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAACCg  >  2:536465/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
CTTCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAACCG  >  CP009273/1993383‑1993675

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CTCTTCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAACCGGTT  >  CP009273/1993381‑1993678
                                                                                                                                                     |                                                                                                                                                    
CTCTTCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTA                                                                                                                                                      <  SRR19973063.85582/150‑1 (MQ=60)
 TCTTCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAA                                                                                                                                                     <  SRR19973063.3665136/150‑1 (MQ=60)
  CTTCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAAT                                                                                                                                                    <  SRR19973063.3190475/150‑1 (MQ=60)
  CTTCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAAT                                                                                                                                                    <  SRR19973063.879381/150‑1 (MQ=60)
  CTTCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAAT                                                                                                                                                    <  SRR19973063.879281/150‑1 (MQ=60)
     CATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAT                                                                                                                                                 <  SRR19973063.683359/150‑1 (MQ=60)
     CATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAG                                                                                                                                                 <  SRR19973063.32751/150‑1 (MQ=60)
     CATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAG                                                                                                                                                 <  SRR19973063.32758/150‑1 (MQ=60)
     CATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAG                                                                                                                                                 >  SRR19973063.3631057/1‑150 (MQ=60)
      ATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTGCGTTACCTTTTTTCACCAGCGCCTGAAAACCAGAAATGGTGTACGGGGTTGAGAAATCGAATTTTTTCTTGCGCTAATCAGA                                                                                                                                                >  SRR19973063.1553688/1‑150 (MQ=60)
         GTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAAT                                                                                                                                             <  SRR19973063.123123/150‑1 (MQ=60)
           TGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGG                                                                                                                                           >  SRR19973063.1030532/1‑150 (MQ=60)
             GTGCCCAGACCGACCCCCACATTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCTTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTG                                                                                                                                         <  SRR19973063.4356210/150‑1 (MQ=60)
              TGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGA                                                                                                                                        <  SRR19973063.2986703/150‑1 (MQ=60)
              TGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGA                                                                                                                                        >  SRR19973063.2230685/1‑150 (MQ=60)
               GCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGAC                                                                                                                                       >  SRR19973063.3352786/1‑150 (MQ=60)
               GCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGAC                                                                                                                                       <  SRR19973063.5143795/150‑1 (MQ=60)
                 CCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCT                                                                                                                                     >  SRR19973063.1822229/1‑150 (MQ=60)
                  CAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTG                                                                                                                                    <  SRR19973063.3453437/150‑1 (MQ=60)
                  CAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTG                                                                                                                                    <  SRR19973063.2072159/150‑1 (MQ=60)
                    GACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGAT                                                                                                                                  <  SRR19973063.2542895/150‑1 (MQ=60)
                      CCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTA                                                                                                                                <  SRR19973063.3149822/150‑1 (MQ=60)
                      CCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTA                                                                                                                                >  SRR19973063.323228/1‑150 (MQ=60)
                        GACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTAAATTGTCCCTTCGTTACCTATTATCACAAGCGCCTGAAAACCACACATGGTGAACGCGGATGAGAAATCGTATTTTTTCTTTCTCTAATCAGAAATGGTGACCTGATTAAT                                                                                                                              >  SRR19973063.2729903/1‑150 (MQ=60)
                           CCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCAC                                                                                                                           >  SRR19973063.3981164/1‑150 (MQ=60)
                           CCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCAC                                                                                                                           <  SRR19973063.3511165/150‑1 (MQ=60)
                             CCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCA                                                                                                                         <  SRR19973063.3192637/150‑1 (MQ=60)
                              CACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCAC                                                                                                                        <  SRR19973063.4310987/150‑1 (MQ=60)
                              CACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCAC                                                                                                                        <  SRR19973063.4507306/150‑1 (MQ=60)
                              CACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCAC                                                                                                                        <  SRR19973063.1725194/150‑1 (MQ=60)
                              CACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCAC                                                                                                                        >  SRR19973063.4261318/1‑150 (MQ=60)
                              CACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCAC                                                                                                                        >  SRR19973063.4261334/1‑150 (MQ=60)
                               ACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACA                                                                                                                       >  SRR19973063.4690794/1‑150 (MQ=60)
                                 TTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATC                                                                                                                     <  SRR19973063.2421976/150‑1 (MQ=60)
                                 TTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATC                                                                                                                     <  SRR19973063.2421990/150‑1 (MQ=60)
                                    TTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAAT                                                                                                                  <  SRR19973063.5069931/150‑1 (MQ=60)
                                    TTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAAT                                                                                                                  >  SRR19973063.1171866/1‑150 (MQ=60)
                                     TTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATA                                                                                                                 <  SRR19973063.3098681/150‑1 (MQ=60)
                                     TTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATA                                                                                                                 >  SRR19973063.123990/1‑150 (MQ=60)
                                      TGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATAC                                                                                                                >  SRR19973063.1480064/1‑150 (MQ=60)
                                         CTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTT                                                                                                             >  SRR19973063.1966498/1‑150 (MQ=60)
                                             CAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGA                                                                                                         <  SRR19973063.239243/150‑1 (MQ=60)
                                              AGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAG                                                                                                        >  SRR19973063.2094467/1‑150 (MQ=60)
                                                  CATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCA                                                                                                    >  SRR19973063.742873/1‑150 (MQ=60)
                                                   ATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGATAATCAGAAATGGTGACCTTATTAATAACCACATCAATACGTTTAGAGTCCAG                                                                                                   >  SRR19973063.4915238/1‑150 (MQ=60)
                                                     CGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCG                                                                                                 <  SRR19973063.3821247/150‑1 (MQ=60)
                                                     CGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCG                                                                                                 >  SRR19973063.2518972/1‑150 (MQ=60)
                                                      GGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGA                                                                                                >  SRR19973063.4963580/1‑150 (MQ=60)
                                                      GGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGA                                                                                                >  SRR19973063.4594043/1‑150 (MQ=60)
                                                      GGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGA                                                                                                <  SRR19973063.4335375/150‑1 (MQ=60)
                                                          GTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCC                                                                                            >  SRR19973063.3112114/1‑150 (MQ=60)
                                                          GTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCC                                                                                            <  SRR19973063.3170408/150‑1 (MQ=60)
                                                          GTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCC                                                                                            <  SRR19973063.3170572/150‑1 (MQ=60)
                                                           TTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCA                                                                                           <  SRR19973063.767004/150‑1 (MQ=60)
                                                           TTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCA                                                                                           <  SRR19973063.3950209/150‑1 (MQ=60)
                                                           TTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCA                                                                                           <  SRR19973063.767107/150‑1 (MQ=60)
                                                             TTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGC                                                                                         >  SRR19973063.4597963/1‑150 (MQ=60)
                                                                ATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATA                                                                                      >  SRR19973063.549444/1‑150 (MQ=60)
                                                                ATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATA                                                                                      >  SRR19973063.2293100/1‑150 (MQ=60)
                                                                  GGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACC                                                                                    <  SRR19973063.240123/150‑1 (MQ=60)
                                                                    TGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACAAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTATCTTGAGCTAATCAGAAATGTTGACCTGATTAATCACCACATCAATAAGTTTAGAGTCAAGCGACGCCAGCATACCGT                                                                                  >  SRR19973063.3720399/1‑150 (MQ=60)
                                                                     GCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTC                                                                                 <  SRR19973063.1713272/150‑1 (MQ=60)
                                                                      CCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCC                                                                                >  SRR19973063.5080071/1‑150 (MQ=60)
                                                                      CCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCC                                                                                >  SRR19973063.5080061/1‑150 (MQ=60)
                                                                        TTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGTGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCA                                                                              <  SRR19973063.2230775/150‑1 (MQ=60)
                                                                        TTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCA                                                                              <  SRR19973063.5087700/150‑1 (MQ=60)
                                                                        TTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCA                                                                              <  SRR19973063.2369047/150‑1 (MQ=60)
                                                                           GTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTT                                                                           >  SRR19973063.4867287/1‑150 (MQ=60)
                                                                           GTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGGTTAGAGTCCAGCGACGCCAGCATACCGTCCCAGTT                                                                           >  SRR19973063.4867174/1‑150 (MQ=60)
                                                                             TACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGG                                                                         <  SRR19973063.2444638/150‑1 (MQ=60)
                                                                              ACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGT                                                                        <  SRR19973063.1798923/150‑1 (MQ=60)
                                                                              ACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGT                                                                        >  SRR19973063.2983352/1‑150 (MQ=60)
                                                                              ACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGT                                                                        >  SRR19973063.3560642/1‑150 (MQ=60)
                                                                                 TTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGG                                                                     >  SRR19973063.1578868/1‑150 (MQ=60)
                                                                                 TTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGG                                                                     >  SRR19973063.1578765/1‑150 (MQ=60)
                                                                                 TTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGG                                                                     >  SRR19973063.3156484/1‑150 (MQ=60)
                                                                                   TTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTT                                                                   >  SRR19973063.4239215/1‑150 (MQ=60)
                                                                                   TTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTT                                                                   <  SRR19973063.168174/150‑1 (MQ=60)
                                                                                   TTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTT                                                                   <  SRR19973063.168169/150‑1 (MQ=60)
                                                                                     TTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTT                                                                 <  SRR19973063.66129/150‑1 (MQ=60)
                                                                                     TTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTT                                                                 <  SRR19973063.1599416/150‑1 (MQ=60)
                                                                                     TTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTT                                                                 <  SRR19973063.4176323/150‑1 (MQ=60)
                                                                                      TCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTA                                                                <  SRR19973063.3356577/150‑1 (MQ=60)
                                                                                      TCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTA                                                                <  SRR19973063.4756793/150‑1 (MQ=60)
                                                                                       CACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAG                                                               <  SRR19973063.3965506/150‑1 (MQ=60)
                                                                                        ACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGT                                                              >  SRR19973063.3441084/1‑150 (MQ=60)
                                                                                          CAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGA                                                            >  SRR19973063.1268840/1‑150 (MQ=60)
                                                                                          CAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGA                                                            >  SRR19973063.1268865/1‑150 (MQ=60)
                                                                                           AGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGAC                                                           >  SRR19973063.4080690/1‑150 (MQ=60)
                                                                                            GCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACG                                                          <  SRR19973063.1676859/150‑1 (MQ=60)
                                                                                             CGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGC                                                         >  SRR19973063.3620897/1‑150 (MQ=60)
                                                                                             CGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGC                                                         >  SRR19973063.3620599/1‑150 (MQ=60)
                                                                                               CCTGAATACCAGACATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCT                                                       <  SRR19973063.3054332/150‑1 (MQ=60)
                                                                                               CCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCT                                                       <  SRR19973063.4002487/150‑1 (MQ=60)
                                                                                                 TGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCA                                                     >  SRR19973063.1844614/1‑150 (MQ=60)
                                                                                                   AATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAAC                                                   >  SRR19973063.4549511/1‑150 (MQ=60)
                                                                                                   AATAACAGAACTGGTGTACGGGTTTGATAAATCGTATTTTTTCTTGCGATAATCATAAATTGTGACCTGATTAATCACCACATCAATACGTTTATAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACTCCTCAAC                                                   <  SRR19973063.4789268/150‑1 (MQ=60)
                                                                                                     TACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGC                                                 >  SRR19973063.2777628/1‑150 (MQ=60)
                                                                                                       CCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCA                                               <  SRR19973063.2332211/150‑1 (MQ=60)
                                                                                                       CCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCA                                               >  SRR19973063.4478668/1‑150 (MQ=60)
                                                                                                         AGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAG                                             <  SRR19973063.4502561/150‑1 (MQ=60)
                                                                                                            AATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATG                                          >  SRR19973063.165366/1‑150 (MQ=60)
                                                                                                               GGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTT                                       <  SRR19973063.534273/150‑1 (MQ=60)
                                                                                                                GTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACACCAAGATGTTTT                                      <  SRR19973063.4061130/150‑1 (MQ=60)
                                                                                                                    ACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCA                                  >  SRR19973063.4673772/1‑150 (MQ=60)
                                                                                                                        GGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTG                              >  SRR19973063.645392/1‑150 (MQ=60)
                                                                                                                        GGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTG                              >  SRR19973063.646126/1‑150 (MQ=60)
                                                                                                                        GGTTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTG                              <  SRR19973063.4148316/150‑1 (MQ=60)
                                                                                                                          TTGAGAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTT                            <  SRR19973063.1807615/150‑1 (MQ=60)
                                                                                                                              GAAATCGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGC                        <  SRR19973063.3565326/150‑1 (MQ=60)
                                                                                                                                   CGTATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATT                   >  SRR19973063.4821298/1‑150 (MQ=60)
                                                                                                                                      ATTTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCA                >  SRR19973063.1750502/1‑150 (MQ=60)
                                                                                                                                        TTTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACT              >  SRR19973063.1742038/1‑150 (MQ=60)
                                                                                                                                         TTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTT             <  SRR19973063.2538514/150‑1 (MQ=60)
                                                                                                                                         TTTTCTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTT             <  SRR19973063.2063242/150‑1 (MQ=60)
                                                                                                                                             CTTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAA         >  SRR19973063.624207/1‑150 (MQ=60)
                                                                                                                                              TTGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAA        <  SRR19973063.379491/150‑1 (MQ=60)
                                                                                                                                               TGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAAC       >  SRR19973063.4739395/1‑150 (MQ=60)
                                                                                                                                               TGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAAC       <  SRR19973063.3102718/150‑1 (MQ=60)
                                                                                                                                               TGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAAC       <  SRR19973063.3102671/150‑1 (MQ=60)
                                                                                                                                               TGCGCTAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAAC       >  SRR19973063.977296/1‑150 (MQ=60)
                                                                                                                                                    TAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAACCGGTT  <  SRR19973063.4892260/150‑1 (MQ=60)
                                                                                                                                                    TAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAACCGGTT  >  SRR19973063.3191430/1‑150 (MQ=60)
                                                                                                                                                    TAATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCACCCCCGCAAATTCCACTTCAAAACCGGTT  <  SRR19973063.4891680/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
CTCTTCATAGTTGGTGCCCAGACCGACCCCCACTTTTTTGCCTTTCAGATCATCGGCTGTTTTAATGGTGCCTTCGTTACCTTTTTTCACCAGCGCCTGAATACCAGAAATGGTGTACGGGGTTGAGAAATCGTATTTTTTCTTGCGCTCATCAGAAATGGTGACCTGATTAATCACCACATCAATACGTTTAGAGTCCAGCGACGCCAGCATACCGTCCCATTTGGTCGGTTTTAGTGACGCCTCAACGCCAAGATGTTTTGCCAGCTGTTGGGCAAATTCCACTTCAAAACCGGTT  >  CP009273/1993381‑1993678

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑