Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F142 I0 R1 859 274.6 8723680 96.4% 8409627 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,380,174 C→A 21.8% S458S (TCG→TCT)  aaeB ← p‑hydroxybenzoic acid efflux system component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,380,1740CA21.8% 765.2 / 170.0 293S458S (TCG→TCT) aaeBp‑hydroxybenzoic acid efflux system component
Reads supporting (aligned to +/- strand):  ref base C (105/124);  new base A (34/30);  total (139/154)
Fisher's exact test for biased strand distribution p-value = 3.24e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.89e-01

Only 100 of 294 total aligned reads displayed.

CGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGAGCAGCCCTAACGGCAGCGCGGCCAGCGTCCC  >  CP009273/3380025‑3380320
                                                                                                                                                     |                                                                                                                                                  
  cgATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATAGa                                                                                                                                                  >  1:2929358/1‑150 (MQ=255)
     tttATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGTAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGcc                                                                                                                                               <  2:4337100/150‑1 (MQ=255)
       tATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCa                                                                                                                                             <  2:1178033/150‑1 (MQ=255)
          cccGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTc                                                                                                                                          <  2:1752061/150‑1 (MQ=255)
            cGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATAGAGCCCAGTCGc                                                                                                                                        >  2:933533/1‑150 (MQ=255)
            cGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATAGAGCCCAGTCGc                                                                                                                                        >  2:4140307/1‑150 (MQ=255)
               aCCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGt                                                                                                                                     >  1:2765217/1‑150 (MQ=255)
                ccAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGtt                                                                                                                                    >  2:1518715/1‑150 (MQ=255)
                 cAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGttt                                                                                                                                   <  1:311102/150‑1 (MQ=255)
                   gCAAAATCACGGTGACCGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGATATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTCTGGTGCTGGCCAGTGCCCCCATAGAGCCCAGTCGCCGTTTCt                                                                                                                                 <  1:27507/150‑1 (MQ=255)
                   gCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCt                                                                                                                                 <  1:140336/150‑1 (MQ=255)
                   gCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCt                                                                                                                                 <  1:1566700/150‑1 (MQ=255)
                        aTAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATAGAGCCCAGTCGCCGTTTCTGTACt                                                                                                                            <  2:829415/150‑1 (MQ=255)
                          aaCGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGGACGATAATATTTATGGTGCTGGCCAGTGCCCCCATAGAGCCCAGTCGCCGTTTCTGTACTTc                                                                                                                          >  2:2160941/1‑150 (MQ=255)
                            cGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCta                                                                                                                        <  1:1592431/150‑1 (MQ=255)
                            cGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCta                                                                                                                        <  1:3782057/150‑1 (MQ=255)
                            cGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCta                                                                                                                        <  1:440607/150‑1 (MQ=255)
                                gAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATAcc                                                                                                                    >  2:627602/1‑150 (MQ=255)
                                          acacaGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAAt                                                                                                          >  1:2098966/1‑150 (MQ=255)
                                            acaGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATcc                                                                                                        <  1:2296920/150‑1 (MQ=255)
                                             caGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATccc                                                                                                       <  1:2457063/150‑1 (MQ=255)
                                             caGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATAGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATccc                                                                                                       >  2:2688608/1‑150 (MQ=255)
                                              aGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCa                                                                                                      >  1:122742/1‑150 (MQ=255)
                                                ccGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATAGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGc                                                                                                    >  1:1264514/1‑150 (MQ=255)
                                                  gacgaTTTGCCCTAATGCGCTGTCGAGAAACTGACCGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGccc                                                                                                  <  1:2417472/150‑3 (MQ=255)
                                                    cgaTTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACta                                                                                                <  1:4130733/150‑2 (MQ=255)
                                                      aTTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGcc                                                                                              >  1:1774686/1‑150 (MQ=255)
                                                      aTTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGcc                                                                                              >  1:951712/1‑150 (MQ=255)
                                                       tttGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAACCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCa                                                                                             <  2:2898599/150‑1 (MQ=255)
                                                        ttGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAg                                                                                            <  1:2732606/150‑1 (MQ=255)
                                                        ttGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATAGAGCCCAGTCGCCGTTTCTGTTCTTCTATACCGAGGAAGAATCCCAGCACTGCCAg                                                                                            >  1:2915780/1‑150 (MQ=255)
                                                         tGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAgg                                                                                           <  1:2237764/150‑1 (MQ=255)
                                                          gCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATAGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGc                                                                                          >  2:535774/1‑150 (MQ=255)
                                                           cccTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCt                                                                                         <  1:581901/150‑1 (MQ=255)
                                                            ccTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCtc                                                                                        >  2:3209118/1‑149 (MQ=255)
                                                            ccTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTa                                                                                        >  2:747466/1‑150 (MQ=255)
                                                              tAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAAt                                                                                      <  2:1916308/150‑1 (MQ=255)
                                                               aaTGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATg                                                                                     <  1:540725/150‑1 (MQ=255)
                                                                 tGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGca                                                                                   <  1:1652747/150‑1 (MQ=255)
                                                                         cGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAgcagca                                                                           >  1:1936587/1‑150 (MQ=255)
                                                                           agaAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATAGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAAc                                                                         >  1:3177734/1‑150 (MQ=255)
                                                                             aaaCTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACAt                                                                       >  1:1665536/1‑150 (MQ=255)
                                                                             aaaCTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACAt                                                                       >  1:51749/1‑150 (MQ=255)
                                                                              aaCTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATg                                                                      >  2:240332/1‑150 (MQ=255)
                                                                               actgactgAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGc                                                                     <  1:1991907/150‑1 (MQ=255)
                                                                               actgactgAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATAGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGc                                                                     >  1:4214195/1‑150 (MQ=255)
                                                                                 tgactgaAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGctc                                                                   <  2:981804/150‑1 (MQ=255)
                                                                                  gactgaAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGctct                                                                  <  1:4064415/150‑1 (MQ=255)
                                                                                  gactgaAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGctct                                                                  <  2:1404639/150‑1 (MQ=255)
                                                                                  gactgaAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATAGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGctct                                                                  >  1:2319441/1‑150 (MQ=255)
                                                                                      gaAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTg                                                              <  2:3295588/150‑1 (MQ=255)
                                                                                      gaAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTg                                                              <  2:4121478/150‑1 (MQ=255)
                                                                                       aaaTGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTgg                                                             >  1:3878537/1‑150 (MQ=255)
                                                                                        aaTGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTggg                                                            >  2:3434970/1‑150 (MQ=255)
                                                                                        aaTGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTggg                                                            >  2:1044644/1‑150 (MQ=255)
                                                                                         aTGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGt                                                           <  2:1527018/150‑1 (MQ=255)
                                                                                          tGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATAGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTa                                                          <  2:3015578/150‑1 (MQ=255)
                                                                                             aaaGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTa                                                       <  2:3994842/150‑1 (MQ=255)
                                                                                               aGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAATAATACCAGCACAGCCACGCGAATGCACAGCAGCCACAAGCTCTGTTGGGTATTAgg                                                     >  1:366919/1‑150 (MQ=255)
                                                                                                gTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAggg                                                    <  2:1328030/150‑1 (MQ=255)
                                                                                                  cATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCCGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGAt                                                  >  2:155089/1‑150 (MQ=255)
                                                                                                   aTCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTGTGTACTTCTATAACGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATa                                                 >  1:743905/1‑150 (MQ=255)
                                                                                                   aTCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATa                                                 <  1:2570684/150‑1 (MQ=255)
                                                                                                   aTCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATAGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATa                                                 >  1:3918471/1‑150 (MQ=255)
                                                                                                     cGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAAt                                               <  1:2640009/150‑1 (MQ=255)
                                                                                                     cGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAAt                                               <  1:3008814/150‑1 (MQ=255)
                                                                                                      gggTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATc                                              >  2:1122560/1‑150 (MQ=255)
                                                                                                      gggTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATc                                              >  2:769966/1‑150 (MQ=255)
                                                                                                       ggTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCa                                             <  1:1010392/150‑1 (MQ=255)
                                                                                                         ttATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCAcc                                           <  1:398323/150‑1 (MQ=255)
                                                                                                            tCCAGCACGATAATCTATCTGGTGCTGGCCAGTGCCCCCCTCGAGCCCAGTCGCCGTTTCTGTACTTCTAGACCGAGGCAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCACCCTGCTCTGTTGGGTATTAGGGATAATCACCaaa                                        <  2:4273260/150‑1 (MQ=255)
                                                                                                              cAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATAGAGCCCAGTCGCCGTTTCTGTACTTCTATAACGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATTCTCTGTTGGGTATTAGGGATAATCACCaaaaa                                      >  1:1535409/1‑150 (MQ=255)
                                                                                                                    gATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTagag                                <  1:1350559/150‑1 (MQ=255)
                                                                                                                     ataataTTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGagc                               >  1:3545248/1‑150 (MQ=255)
                                                                                                                     ataataTTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGagc                               <  2:1859279/150‑1 (MQ=255)
                                                                                                                      taataTTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTAGACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGagca                              <  1:2681902/150‑1 (MQ=255)
                                                                                                                       aataTTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTTTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATTCACAGCAGCAACATGCTCTGTTGGGTATTATGGATAATCACCAAAAAGTAGagcag                             >  2:2546792/1‑150 (MQ=255)
                                                                                                                       aataTTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGagcag                             >  1:315994/1‑150 (MQ=255)
                                                                                                                       aataTTTATGGTGCTGGCCAGTGCCCCCATAGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGagcag                             <  1:3686849/150‑1 (MQ=255)
                                                                                                                        atatTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGagcagc                            <  1:3479491/150‑1 (MQ=255)
                                                                                                                        atatTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGagcagc                            >  2:1791486/1‑150 (MQ=255)
                                                                                                                         tatTTATGGTGCTGGCCAGTGCCCCCATAGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGAGCAGcc                           >  2:2144015/1‑150 (MQ=255)
                                                                                                                          atTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTCATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGAGCAGccc                          >  2:2778587/1‑150 (MQ=255)
                                                                                                                          atTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACAGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTATAGCAGccc                          >  1:109060/1‑150 (MQ=255)
                                                                                                                           tttATGGTGCTGGCCAGTGCCCCCATAGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGAGCAGCCCt                         <  2:1996138/150‑1 (MQ=255)
                                                                                                                              aTGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGAGCAGCCCTAAc                      <  2:1963290/150‑1 (MQ=255)
                                                                                                                              aTGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGAGCAGCCCTAAc                      <  2:2063690/150‑1 (MQ=255)
                                                                                                                               tGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGAGCAGCCCTAACg                     <  1:3371472/150‑1 (MQ=255)
                                                                                                                                   gCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGAGCAGCCCTAACGGCAg                 <  2:3778814/150‑1 (MQ=255)
                                                                                                                                    cTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTGTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGAGCCGCCCTAACGGCAgc                >  2:2642074/1‑150 (MQ=255)
                                                                                                                                     tGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAAACCCAGCACAGCCAGGCGAATGCACAGCCGCCACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGAGCAGCCCAAACGGCAgcg               >  1:2941728/1‑150 (MQ=255)
                                                                                                                                     tGGCCAGTGCCCCCATAGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGAGCAGCCCTAACGGCAgcg               >  2:3622004/1‑150 (MQ=255)
                                                                                                                                     tGGCCAGTGCCCCCATAGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGAGCAGCCCTAACGGCAgcg               >  2:4200181/1‑150 (MQ=255)
                                                                                                                                      ggCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGAGCAGCCCTAACGGCAgcgc              <  2:2289090/150‑1 (MQ=255)
                                                                                                                                        ccAGTGCCCCCATAGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGAGCAGCCCTAACGGCAGCGCgg            <  1:3173916/150‑1 (MQ=255)
                                                                                                                                         cAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACAAGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGAGCAGCCCTAACGGCAGCGCGGc           >  1:4087075/1‑150 (MQ=255)
                                                                                                                                          aGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGAGCAGCCCTAACGGCAGCGCGGcc          >  2:620631/1‑150 (MQ=255)
                                                                                                                                          aGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGAGCAGCCCAAACGGCAGCGCGGcc          >  1:3078725/1‑150 (MQ=255)
                                                                                                                                            tGCCCCCATAGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGAGCAGCCCTAACGGCAGCGCGGCCAg        >  1:2861088/1‑150 (MQ=255)
                                                                                                                                                  cATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGAGCAGCCCTAACGGCAGCGCGGCCAGCGTccc  >  2:163806/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
CGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGAGCAGCCCTAACGGCAGCGCGGCCAGCGTCCC  >  CP009273/3380025‑3380320

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A