Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,380,174 C→A 36.6% S458S (TCG→TCT)  aaeB ← p‑hydroxybenzoic acid efflux system component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,380,1740CA36.6% 399.8 / 256.6 258S458S (TCG→TCT) aaeBp‑hydroxybenzoic acid efflux system component
Reads supporting (aligned to +/- strand):  ref base C (97/66);  new base A (46/49);  total (143/115)
Fisher's exact test for biased strand distribution p-value = 9.24e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 263 total aligned reads displayed.

CGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGAGCAGCCCTAACGGCAGCGCGGCCAGCGTCCCGT  >  CP009273/3380025‑3380322
                                                                                                                                                     |                                                                                                                                                    
cgcgATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATc                                                                                                                                                      <  1:2136261/150‑1 (MQ=255)
 gcgATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCg                                                                                                                                                     <  1:625854/150‑1 (MQ=255)
 gcgATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCg                                                                                                                                                     <  1:625797/150‑1 (MQ=255)
   gATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATagag                                                                                                                                                   >  2:2327501/1‑150 (MQ=255)
      ttATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGccc                                                                                                                                                <  2:2771958/150‑1 (MQ=255)
       tATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATAGAGCCCa                                                                                                                                               >  2:3511875/1‑150 (MQ=255)
         tCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGt                                                                                                                                             >  2:94124/1‑150 (MQ=255)
         tCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATAGAGCCCAGt                                                                                                                                             <  1:1422877/150‑1 (MQ=255)
          cccGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATAGAGCCCAGTc                                                                                                                                            >  1:2680557/1‑150 (MQ=255)
           ccGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATAGAGCCCAGTCg                                                                                                                                           <  1:2984030/150‑1 (MQ=255)
            cGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGc                                                                                                                                          >  2:3888637/1‑150 (MQ=255)
            cGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGc                                                                                                                                          >  2:768522/1‑150 (MQ=255)
                ccAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGtt                                                                                                                                      <  1:371867/150‑1 (MQ=255)
                 cAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATTGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGttt                                                                                                                                     <  2:3754779/150‑1 (MQ=255)
                   gCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCt                                                                                                                                   <  2:2280551/150‑1 (MQ=255)
                   gCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATAGAGCCCAGTCGCCGTTTCt                                                                                                                                   >  1:2885649/1‑150 (MQ=255)
                   gCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATAGAGCCCAGTCGCCGTTTCt                                                                                                                                   >  1:1966334/1‑150 (MQ=255)
                     aaaaTAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGt                                                                                                                                 <  1:2492783/150‑1 (MQ=255)
                      aaaTAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTa                                                                                                                                >  1:3974528/1‑150 (MQ=255)
                      aaaTAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTa                                                                                                                                >  1:3849843/1‑150 (MQ=255)
                       aaTAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTAc                                                                                                                               <  1:3892692/150‑1 (MQ=255)
                       aaTAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTAc                                                                                                                               >  1:331440/1‑150 (MQ=255)
                        aTAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCCTCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACt                                                                                                                              <  2:1345449/150‑1 (MQ=255)
                         tAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACtt                                                                                                                             <  1:327860/150‑1 (MQ=255)
                         tAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATAGAGCCCAGTCGCCGTTTCTGTACtt                                                                                                                             >  1:2309954/1‑150 (MQ=255)
                          aaCGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTc                                                                                                                            >  2:3128615/1‑150 (MQ=255)
                          aaCGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTc                                                                                                                            >  1:1840290/1‑150 (MQ=255)
                           aCGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATAGAGCCCAGTCGCCGTTTCTGTACTTCt                                                                                                                           <  1:2204767/150‑1 (MQ=255)
                                gAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATAcc                                                                                                                      >  2:1781123/1‑150 (MQ=255)
                                       aGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGgaag                                                                                                               >  1:2331829/1‑150 (MQ=255)
                                        gCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATAGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGgaaga                                                                                                              >  1:686893/1‑150 (MQ=255)
                                          acacaGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGTCTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAAt                                                                                                            <  2:531281/150‑1 (MQ=255)
                                           cacaGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATc                                                                                                           >  1:3406472/1‑150 (MQ=255)
                                             caGCCGACGCTTTGCCCTCCTGCCCTGTCGAGAAACTGACTGAAATGGAAACTCATCGGGTTATCCAGCACGAGAATATGTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGGACGCCTATACCGAGGAAGAATccc                                                                                                         <  2:1510635/150‑1 (MQ=255)
                                              aGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCa                                                                                                        >  1:74527/1‑150 (MQ=255)
                                              aGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATAGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCa                                                                                                        <  1:4127965/150‑1 (MQ=255)
                                                ccGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGc                                                                                                      <  1:3942731/150‑1 (MQ=255)
                                                  gacgaTTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATAGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCAc                                                                                                    >  2:1095609/1‑150 (MQ=255)
                                                   acgaTTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCACATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACt                                                                                                   >  2:885418/1‑150 (MQ=255)
                                                      aTTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGcc                                                                                                >  2:1774183/1‑150 (MQ=255)
                                                      aTTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATAGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGcc                                                                                                >  1:149335/1‑150 (MQ=255)
                                                       tttGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCa                                                                                               <  2:466803/150‑1 (MQ=255)
                                                       tttGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCa                                                                                               <  1:2076463/150‑1 (MQ=255)
                                                        ttGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAg                                                                                              >  2:2303000/1‑150 (MQ=255)
                                                          gCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGc                                                                                            <  2:2642243/150‑1 (MQ=255)
                                                          gCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGc                                                                                            <  2:1272604/150‑1 (MQ=255)
                                                             cTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTaa                                                                                         >  2:1974749/1‑150 (MQ=255)
                                                               aaTGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATg                                                                                       <  2:252687/150‑1 (MQ=255)
                                                                   cgcTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGcaca                                                                                   <  1:890708/150‑1 (MQ=255)
                                                                         cGATAAAATGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCACCATCGAGCCCAGTCGACGTTTCTGTACTTCTATACCGATGTAGAATCACAGCACTGCCAGGCTAATGCAAAgcatca                                                                             <  2:3317372/150‑1 (MQ=255)
                                                                          gagaAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCaa                                                                            >  2:1080482/1‑150 (MQ=255)
                                                                           agaAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATAGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAAc                                                                           >  2:3712517/1‑150 (MQ=255)
                                                                           agaAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATAGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAAc                                                                           <  2:2993931/150‑1 (MQ=255)
                                                                            gaAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACa                                                                          <  1:3676822/150‑1 (MQ=255)
                                                                             aaaCTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACAt                                                                         >  1:2723435/1‑150 (MQ=255)
                                                                                  gactgaAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGctct                                                                    >  2:1896321/1‑150 (MQ=255)
                                                                                  gactgaAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATAGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGctct                                                                    <  1:932028/150‑1 (MQ=255)
                                                                                    ctgaAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATAGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGt                                                                  <  2:3768881/150‑1 (MQ=255)
                                                                                     tgaAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGtt                                                                 >  2:963780/1‑150 (MQ=255)
                                                                                     tgaAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGtt                                                                 >  1:3660989/1‑150 (MQ=255)
                                                                                        aaTGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATAGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTggg                                                              >  1:2544783/1‑150 (MQ=255)
                                                                                          tGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTa                                                            >  2:1956292/1‑150 (MQ=255)
                                                                                           ggAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTAt                                                           >  2:3543486/1‑150 (MQ=255)
                                                                                           ggAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTAt                                                           >  1:4042099/1‑150 (MQ=255)
                                                                                              aaGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAg                                                        >  2:2581308/1‑150 (MQ=255)
                                                                                                 tCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGa                                                     >  2:1693889/1‑150 (MQ=255)
                                                                                                 tCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGa                                                     >  2:2659907/1‑150 (MQ=255)
                                                                                                  cATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGAt                                                    >  1:1742725/1‑150 (MQ=255)
                                                                                                  cATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGAt                                                    >  1:305697/1‑150 (MQ=255)
                                                                                                  cATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATAGAGCCCAGTCGCCGTTTCTGTACTTCTATAACGAGGAAGAAAACCAGCACTGCCAGGCTAATGCACAGCAGCAAAATGATCTGTTGTGTATTAGGGAt                                                    >  1:1124111/1‑150 (MQ=255)
                                                                                                       ggTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATAGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCa                                               >  1:2231425/1‑150 (MQ=255)
                                                                                                         ttATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATAGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCAcc                                             <  2:3413952/150‑1 (MQ=255)
                                                                                                              cAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCaaaaa                                        >  2:2170236/1‑150 (MQ=255)
                                                                                                              cAGCACGATAATATTTATGGTGCTGGCCAGGGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCaaaaa                                        <  2:2090505/150‑1 (MQ=255)
                                                                                                               aGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATAGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAg                                       >  1:3613910/1‑150 (MQ=255)
                                                                                                               aGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATAGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAg                                       >  2:3771845/1‑150 (MQ=255)
                                                                                                                 cACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTa                                     <  2:1065262/150‑1 (MQ=255)
                                                                                                                 cACGATAATATTTATGGTGCTGGCCAGTGCCCCCATAGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTa                                     <  2:3984156/150‑1 (MQ=255)
                                                                                                                    gATAATATTTATGGTGCTGGCCAGTGCCCCCATAGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTagag                                  >  1:1099588/1‑150 (MQ=255)
                                                                                                                     ataataTTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGagc                                 <  2:2954828/150‑1 (MQ=255)
                                                                                                                     ataataTTTATGGTGCTGGCCAGTGCCCCCATAGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGagc                                 >  1:3229401/1‑150 (MQ=255)
                                                                                                                         tatTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGAGCAGcc                             >  2:4020518/1‑150 (MQ=255)
                                                                                                                          atTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGAGCAGccc                            <  1:3991729/150‑1 (MQ=255)
                                                                                                                            ttATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGAGCAGCCCTa                          <  1:3641838/150‑1 (MQ=255)
                                                                                                                               tGGTGCTGGCCAGTGCCCCCATAGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGAGCAGCCCTAACg                       <  1:2626534/150‑1 (MQ=255)
                                                                                                                                 gTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGAGCAGCCCTAACGGc                     >  2:3048565/1‑150 (MQ=255)
                                                                                                                                 gTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGAGCAGCCCTAACGGc                     >  2:4036411/1‑150 (MQ=255)
                                                                                                                                       gCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGAGCAGCCCTAACGGCAGcgcg               <  2:367641/150‑1 (MQ=255)
                                                                                                                                        ccAGTGCCCCCATAGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGAGCAGCCCTAACGGCAGCGCgg              <  2:686811/150‑1 (MQ=255)
                                                                                                                                         cAGTGCCCCCATAGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGAGCAGCCCTAACGGCAGCGCGGc             >  2:3270887/1‑150 (MQ=255)
                                                                                                                                           gTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGAGCAGCCCTAACGGCAGCGCGGCCa           >  1:811049/1‑150 (MQ=255)
                                                                                                                                           gTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGAGCAGCCCTAACGGCAGCGCGGCCa           <  1:3874216/150‑1 (MQ=255)
                                                                                                                                           gTGCCCCCATAGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGAGCAGCCCTAACGGCAGCGCGGCCa           <  2:1589789/150‑1 (MQ=255)
                                                                                                                                            tGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGAGCAGCCCTAACGGCAGCGCGGCCAg          <  2:1042750/150‑1 (MQ=255)
                                                                                                                                             gCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGAGCAGCCCTAACGGCAGCGCGGCCAGc         >  1:3464200/1‑150 (MQ=255)
                                                                                                                                              cccccATCGAGCCCAGTCGCCGTTTCTGAACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGCTAATCACCAAAAAGTAGAGCAGCCCTAACGGCAGCGCGGCCAGCg        >  2:3855223/1‑150 (MQ=255)
                                                                                                                                                 ccATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGAGCAGCCCTAACGGCAGCGCGGCCAGCGTcc     >  1:35775/1‑150 (MQ=255)
                                                                                                                                                  cATAGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGAGCAGCCCTAACGGCAGCGCGGCCAGCGTccc    <  2:872370/150‑1 (MQ=255)
                                                                                                                                                  cATAGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGAGCAGCCCTAACGGCAGCGCGGCCAGCGTccc    <  2:3852543/150‑1 (MQ=255)
                                                                                                                                                  cATAGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACGGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGAGCAGCCCTAACGGCAGCGCGGCCAGCGTccc    <  2:500353/150‑1 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CGCGATTTATCCCGCACCAGCAAAATAACGGTGAACGCGAGCACACAGCCGACGATTTGCCCTAATGCGCTGTCGAGAAACTGACTGAAATGGAAAGTCATCGGGTTATCCAGCACGATAATATTTATGGTGCTGGCCAGTGCCCCCATCGAGCCCAGTCGCCGTTTCTGTACTTCTATACCGAGGAAGAATCCCAGCACTGCCAGGCTAATGCACAGCAGCAACATGCTCTGTTGGGTATTAGGGATAATCACCAAAAAGTAGAGCAGCCCTAACGGCAGCGCGGCCAGCGTCCCGT  >  CP009273/3380025‑3380322

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A