Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F527 I0 R1 1651 322.4 10661268 95.2% 10149527 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,381,943 C→A 27.7% T181T (ACG→ACT)  aaeA ← p‑hydroxybenzoic acid efflux system component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,381,9430CA27.7% 679.1 / 217.6 327T181T (ACG→ACT) aaeAp‑hydroxybenzoic acid efflux system component
Reads supporting (aligned to +/- strand):  ref base C (122/114);  new base A (53/38);  total (175/152)
Fisher's exact test for biased strand distribution p-value = 3.23e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.33e-01

Only 100 of 340 total aligned reads displayed.

GCGGCAACACTATCAACAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAACCGTTTG  >  CP009273/3381794‑3382092
                                                                                                                                                     |                                                                                                                                                     
  ggCAACACTATCAACAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGt                                                                                                                                                     >  2:3289624/1‑150 (MQ=255)
   gCAACACTATCAACAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGtt                                                                                                                                                    <  1:4600841/150‑1 (MQ=255)
   gCAACACTATCAACAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGtt                                                                                                                                                    <  2:1028049/150‑1 (MQ=255)
   gCAACACTATCAACAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGtt                                                                                                                                                    <  1:3956054/150‑1 (MQ=255)
    cAACACTATCAACAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTg                                                                                                                                                   >  1:3722252/1‑150 (MQ=255)
      acacTATCAACAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGAt                                                                                                                                                 <  2:169012/150‑1 (MQ=255)
       cacTATCAACAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCAGTTGATc                                                                                                                                                <  1:1835456/150‑1 (MQ=255)
        acTATCAACAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATcc                                                                                                                                               >  1:3276519/1‑150 (MQ=255)
         cTATCAACAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCAGTTGATCCt                                                                                                                                              <  2:1114030/150‑1 (MQ=255)
           aTCAACAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCg                                                                                                                                            <  2:3363453/150‑1 (MQ=255)
           aTCAACAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCg                                                                                                                                            <  2:2312907/150‑1 (MQ=255)
              aaCAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCCGCGCAACCGCCGTTGATCCTCGAGt                                                                                                                                         >  2:2061083/1‑150 (MQ=255)
                  gTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGtaata                                                                                                                                     >  1:2159372/1‑150 (MQ=255)
                   tCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCAGTTGATCCTCGAGtaataa                                                                                                                                    <  1:2608621/150‑1 (MQ=255)
                    cccTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATaaa                                                                                                                                   >  2:4640474/1‑150 (MQ=255)
                     ccTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAAc                                                                                                                                  >  2:4365637/1‑150 (MQ=255)
                     ccTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAAc                                                                                                                                  >  2:4152957/1‑150 (MQ=255)
                     ccTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCAGTTGATCCTCGAGTAATAAAc                                                                                                                                  <  2:3908699/150‑1 (MQ=255)
                      cTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACt                                                                                                                                 <  1:1601241/150‑1 (MQ=255)
                        ttCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCa                                                                                                                               <  1:4884216/150‑1 (MQ=255)
                          cAGCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCAcc                                                                                                                             <  1:1019150/150‑1 (MQ=255)
                          cAGCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCAGTTGATCCTCGAGTAATAAACTCAcc                                                                                                                             >  1:723036/1‑150 (MQ=255)
                            gCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCgg                                                                                                                           >  2:2653735/1‑150 (MQ=255)
                                 ttGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAg                                                                                                                      <  2:3639324/150‑1 (MQ=255)
                                  tGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGa                                                                                                                     <  1:5041427/150‑1 (MQ=255)
                                    ttACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACg                                                                                                                   <  2:926852/150‑1 (MQ=255)
                                     tACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCAGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGt                                                                                                                  <  1:4371814/150‑1 (MQ=255)
                                       cTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCATAGAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTg                                                                                                                <  1:1773506/150‑1 (MQ=255)
                                            aaGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGtt                                                                                                           <  1:2833259/150‑1 (MQ=255)
                                             aGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTg                                                                                                          <  1:589819/150‑1 (MQ=255)
                                             aGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCAGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTg                                                                                                          <  2:3201840/150‑1 (MQ=255)
                                                 gcgTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCa                                                                                                      <  1:5282882/150‑1 (MQ=255)
                                                  cgTGATCACTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCATTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGacac                                                                                                     >  2:884212/1‑150 (MQ=255)
                                                     gATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCa                                                                                                  >  2:3711645/1‑150 (MQ=255)
                                                     gATATCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTATTCCATATAGTCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCAGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCa                                                                                                  >  2:4472612/1‑150 (MQ=255)
                                                      aTCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAg                                                                                                 <  1:2022946/150‑1 (MQ=255)
                                                      aTCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCAGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAg                                                                                                 >  2:505782/1‑150 (MQ=255)
                                                       tctcTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTTCATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGc                                                                                                <  1:1748191/150‑1 (MQ=255)
                                                         tctGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCAGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGCCACCCAGCCa                                                                                              <  2:4125751/150‑1 (MQ=255)
                                                                  tACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCATAGAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAACCTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGgc                                                                                     <  2:2938838/150‑1 (MQ=255)
                                                                      ccGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGgcgcgc                                                                                 <  1:2067411/150‑1 (MQ=255)
                                                                      ccGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCAGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGgcgcgc                                                                                 >  1:3453349/1‑150 (MQ=255)
                                                                        ggACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCgg                                                                               >  1:53241/1‑150 (MQ=255)
                                                                        ggACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCgg                                                                               >  2:4030860/1‑150 (MQ=255)
                                                                        ggACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCgg                                                                               <  1:930009/150‑1 (MQ=255)
                                                                          aCGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGAt                                                                             <  1:4640474/150‑1 (MQ=255)
                                                                            gCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCa                                                                           <  1:3602421/150‑1 (MQ=255)
                                                                              aCCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCAGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCAcc                                                                         <  1:1153278/150‑1 (MQ=255)
                                                                                cccTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGt                                                                       <  1:1105886/150‑1 (MQ=255)
                                                                                 ccTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTg                                                                      <  1:2231136/150‑1 (MQ=255)
                                                                                 ccTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCAGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTg                                                                      <  1:2488940/150‑1 (MQ=255)
                                                                                  cTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCAGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGc                                                                     <  1:4854492/150‑1 (MQ=255)
                                                                                  cTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCAGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGc                                                                     >  2:144396/1‑150 (MQ=255)
                                                                                     ccAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGtt                                                                  <  1:4150938/150‑1 (MQ=255)
                                                                                      cAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTc                                                                 <  2:4789342/150‑1 (MQ=255)
                                                                                      cAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCAGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTc                                                                 <  1:3742436/150‑1 (MQ=255)
                                                                                      cAGCTTAGTTTCTTCCATATAGGCCAGCACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTc                                                                 >  2:2156187/1‑150 (MQ=255)
                                                                                       aGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCa                                                                >  2:3884890/1‑150 (MQ=255)
                                                                                         cTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCATCGCAACCGCCGTTGATCCTCGATTAATAAACTCACAGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAg                                                              >  2:2673764/1‑150 (MQ=255)
                                                                                           tAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGAt                                                            >  2:1981075/1‑150 (MQ=255)
                                                                                            aGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCAGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATc                                                           <  2:2449111/150‑1 (MQ=255)
                                                                                                tcttcCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGTGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGt                                                       <  2:1221875/150‑1 (MQ=255)
                                                                                                 cttcCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGtt                                                      >  1:44739/1‑150 (MQ=255)
                                                                                                 cttcCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCAGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGtt                                                      >  2:2722918/1‑150 (MQ=255)
                                                                                                  ttcCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGttt                                                     <  2:3393394/150‑1 (MQ=255)
                                                                                                    ccATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTg                                                   <  1:3427415/150‑1 (MQ=255)
                                                                                                      atatAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCAGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGcc                                                 >  1:3940082/1‑150 (MQ=255)
                                                                                                      atatAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCAGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGcc                                                 >  2:583635/1‑150 (MQ=255)
                                                                                                           ggCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATc                                            <  2:1352494/150‑1 (MQ=255)
                                                                                                             ccAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATcgc                                          >  2:495376/1‑150 (MQ=255)
                                                                                                              cAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATcgcg                                         <  2:2849007/150‑1 (MQ=255)
                                                                                                              cAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCAGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATcgcg                                         >  2:3762417/1‑150 (MQ=255)
                                                                                                                    aTAGAAGGAGTTCTGTTTCACCAGCGCAACCGCAGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGc                                   >  2:1408709/1‑150 (MQ=255)
                                                                                                                     tAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGcc                                  >  1:2285907/1‑150 (MQ=255)
                                                                                                                       gAAGGAGTTCTGTTTCACCAGCGCAACCGCAGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTg                                >  2:5164728/1‑150 (MQ=255)
                                                                                                                        aaGGAGTTCTGTTTCACCAGCGCAACCGCAGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTgc                               <  1:2785090/150‑1 (MQ=255)
                                                                                                                        aaGGAGTTCTGTTTCACCAGCGCAACCGCAGGTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTgc                               <  1:1513441/150‑1 (MQ=255)
                                                                                                                         aGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTgcg                              >  1:2771000/1‑150 (MQ=255)
                                                                                                                          ggAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTgcgc                             >  2:2217575/1‑150 (MQ=255)
                                                                                                                           gAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCt                            >  1:3774945/1‑150 (MQ=255)
                                                                                                                           gAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTAACCGGTATAGACGTTGAGCTTGGTCACCCAGCCATCTGCTGGCGCGCGCATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCt                            >  2:4574425/1‑150 (MQ=255)
                                                                                                                              ttCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTc                         <  2:1613850/150‑1 (MQ=255)
                                                                                                                                 tGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCt                      >  2:1803878/1‑150 (MQ=255)
                                                                                                                                  gTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGcga                     >  1:4077462/1‑148 (MQ=255)
                                                                                                                                  gTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTa                     <  2:2610092/150‑1 (MQ=255)
                                                                                                                                  gTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTa                     <  1:4504582/150‑1 (MQ=255)
                                                                                                                                   tttCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTaa                    >  2:3164050/1‑150 (MQ=255)
                                                                                                                                      cACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTg                 <  1:814660/150‑1 (MQ=255)
                                                                                                                                      cACCAGCGCAACCGCAGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTg                 >  1:1947065/1‑150 (MQ=255)
                                                                                                                                        ccAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGAt               >  1:4798950/1‑150 (MQ=255)
                                                                                                                                        ccAGCGCAACCGCAGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGAt               <  1:2396394/150‑1 (MQ=255)
                                                                                                                                          aGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGc             <  1:1357029/150‑1 (MQ=255)
                                                                                                                                             gcAACCGCAGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTGTCGCTAACTGATGCAGa          >  1:307914/1‑150 (MQ=255)
                                                                                                                                              cAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGaa         <  1:3201163/150‑1 (MQ=255)
                                                                                                                                               aaCCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAAc        <  1:3181500/150‑1 (MQ=255)
                                                                                                                                               aaCCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAAc        >  2:3503438/1‑150 (MQ=255)
                                                                                                                                                aCCGCAGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAAcc       <  2:667157/150‑1 (MQ=255)
                                                                                                                                                 ccgcagTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAACCg      <  2:3940082/150‑1 (MQ=255)
                                                                                                                                                  cgccgTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAACCGt     >  2:1884952/1‑150 (MQ=255)
                                                                                                                                                   gccgTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCCGCGCTTTCGCTAACTGATGCAGAACCGtt    >  1:4270736/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
GCGGCAACACTATCAACAGTCCCTTTCAGCACTTTGTTACTGCCAAGCGGCGTGATCTCTGCACGATACCCCGGACGCACCCCTTCCAGCTTAGTTTCTTCCATATAGGCCAGTACATAGAAGGAGTTCTGTTTCACCAGCGCAACCGCCGTTGATCCTCGAGTAATAAACTCACCGGTATAGACGTTGAGGTTGGTCACCCAGCCATCTGCTGGCGCGCGGATCACCGTGCGTTCAAGATCCAGTTTTGCCAGATCGCGGGTCGCCTGCGCTTTCGCTAACTGATGCAGAACCGTTTG  >  CP009273/3381794‑3382092

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A