Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 524,496 T→G 100% pseudogene (401/491 nt) ybbD → pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273524,4960TG99.6% 968.7 / ‑5.5 237pseudogene (401/491 nt)ybbDpseudogene
Reads supporting (aligned to +/- strand):  ref base T (0/1);  new base G (123/113);  total (123/114)
Fisher's exact test for biased strand distribution p-value = 4.81e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 238 total aligned reads displayed.

CCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGAGG  >  CP009273/524351‑524639
                                                                                                                                                 |                                                                                                                                               
  cTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTaa                                                                                                                                             <  2:1385730/148‑1 (MQ=255)
  cTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTaa                                                                                                                                             >  2:2491632/1‑148 (MQ=255)
   tAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTaaa                                                                                                                                            >  2:2176381/1‑148 (MQ=255)
   tAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTaaa                                                                                                                                            >  1:2752026/1‑148 (MQ=255)
   tAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTaaa                                                                                                                                            >  1:2656643/1‑148 (MQ=255)
   tAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTaaa                                                                                                                                            >  2:3351160/1‑148 (MQ=255)
   tAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTaaa                                                                                                                                            >  1:1521824/1‑148 (MQ=255)
     aCAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAAcc                                                                                                                                          <  2:1048150/148‑1 (MQ=255)
     aCAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAAcc                                                                                                                                          >  1:13254/1‑148 (MQ=255)
          tgatgatgGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCttttt                                                                                                                                     <  1:2142681/148‑1 (MQ=255)
          tgatgatgGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCttttt                                                                                                                                     >  2:1651435/1‑148 (MQ=255)
           gatgatgGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCtttttt                                                                                                                                    >  1:3404018/1‑148 (MQ=255)
             tgatgGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCtttttttt                                                                                                                                  >  2:1856460/1‑148 (MQ=255)
              gatgGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTttg                                                                                                                                 >  2:1467899/1‑148 (MQ=255)
              gatgGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTttg                                                                                                                                 <  1:2229539/148‑1 (MQ=255)
               atgGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTttgt                                                                                                                                >  2:780987/1‑148 (MQ=255)
                  ggggTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTgg                                                                                                                             <  2:953218/148‑1 (MQ=255)
                   gggTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGt                                                                                                                            >  2:2926884/1‑148 (MQ=255)
                     gTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGa                                                                                                                          <  1:1390531/148‑1 (MQ=255)
                      tAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGaa                                                                                                                         >  2:639214/1‑148 (MQ=255)
                      tAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGaa                                                                                                                         <  2:1730130/148‑1 (MQ=255)
                       aGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGaaa                                                                                                                        <  1:2203177/148‑1 (MQ=255)
                         gTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAAtt                                                                                                                      <  1:3369890/148‑1 (MQ=255)
                         gTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAAtt                                                                                                                      >  2:3496691/1‑148 (MQ=255)
                         gTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAAtt                                                                                                                      >  1:2364835/1‑148 (MQ=255)
                          tttAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTa                                                                                                                     <  2:252842/148‑1 (MQ=255)
                          tttAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTa                                                                                                                     <  2:2025620/148‑1 (MQ=255)
                            tAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAAt                                                                                                                   <  1:1999380/148‑1 (MQ=255)
                             aaGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATg                                                                                                                  <  1:1845615/148‑1 (MQ=255)
                              aGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGt                                                                                                                 <  1:3312775/148‑1 (MQ=255)
                               gAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGtt                                                                                                                <  2:1989327/148‑1 (MQ=255)
                                    cATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCa                                                                                                           >  1:352031/1‑148 (MQ=255)
                                      ttGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCatt                                                                                                         <  1:1159506/148‑1 (MQ=255)
                                          cAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTAttt                                                                                                     <  1:1058304/148‑1 (MQ=255)
                                                 ggCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACa                                                                                              >  2:2806334/1‑148 (MQ=255)
                                                  gCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACaa                                                                                             <  1:2104872/148‑1 (MQ=255)
                                                    cAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAat                                                                                           <  1:3254807/148‑1 (MQ=255)
                                                      gggAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAatat                                                                                         >  1:945039/1‑148 (MQ=255)
                                                       ggAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATAtt                                                                                        <  1:2410060/148‑1 (MQ=255)
                                                          aCAAGTGGCGAAAATCGTATCAAATAATGATCCAGATACAAAAAAAGTGTGGTGTAAATAAGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATTTTACGCATTATATTATACCAAATATTGGa                                                                                     >  1:1412851/1‑148 (MQ=255)
                                                          aCAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGa                                                                                     <  2:3094745/148‑1 (MQ=255)
                                                                ggCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGa                                                                               <  2:1106073/148‑1 (MQ=255)
                                                                ggCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGa                                                                               <  1:723825/148‑1 (MQ=255)
                                                                 gCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGAt                                                                              >  1:1663053/1‑148 (MQ=255)
                                                                 gCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGAt                                                                              >  1:2788699/1‑148 (MQ=255)
                                                                 gCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGAt                                                                              >  1:925139/1‑148 (MQ=255)
                                                                  cGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGAtt                                                                             >  1:3352768/1‑148 (MQ=255)
                                                                    aaaaTCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGc                                                                           <  1:3423595/148‑1 (MQ=255)
                                                                    aaaaTCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGc                                                                           <  1:2515911/148‑1 (MQ=255)
                                                                    aaaaTCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGc                                                                           >  1:2169738/1‑148 (MQ=255)
                                                                    aaaaTCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGc                                                                           >  1:61980/1‑148 (MQ=255)
                                                                    aaaaTCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGc                                                                           <  2:525713/148‑1 (MQ=255)
                                                                     aaaTCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTGTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGcc                                                                          >  2:2899187/1‑148 (MQ=255)
                                                                       aTCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTg                                                                        >  1:150168/1‑148 (MQ=255)
                                                                       aTCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTg                                                                        >  1:977853/1‑148 (MQ=255)
                                                                        tCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGa                                                                       <  2:2354536/148‑1 (MQ=255)
                                                                            aTCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCt                                                                   <  1:1981419/148‑1 (MQ=255)
                                                                                 aGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGc                                                              >  1:2312689/1‑148 (MQ=255)
                                                                                  gAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCa                                                             >  2:2825253/1‑148 (MQ=255)
                                                                                  gAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCa                                                             >  1:554749/1‑148 (MQ=255)
                                                                                     tGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCagag                                                          <  1:2627028/148‑1 (MQ=255)
                                                                                     tGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCagag                                                          >  1:2898267/1‑148 (MQ=255)
                                                                                     tGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCagag                                                          <  1:868673/148‑1 (MQ=255)
                                                                                     tGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCagag                                                          <  1:2039646/148‑1 (MQ=255)
                                                                                      gATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGt                                                         >  1:692091/1‑148 (MQ=255)
                                                                                       aTCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTa                                                        <  2:2859156/148‑1 (MQ=255)
                                                                                         ccAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAAt                                                      >  2:884292/1‑148 (MQ=255)
                                                                                           aGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTg                                                    <  2:2069741/148‑1 (MQ=255)
                                                                                            gATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTTGATTGCCTGATGCTTGTGCAGAGTAATTGc                                                   <  2:1897166/148‑1 (MQ=255)
                                                                                            gATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGc                                                   >  2:3192740/1‑148 (MQ=255)
                                                                                             aTACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCt                                                  <  2:2275722/148‑1 (MQ=255)
                                                                                               aCAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAattgattg                                                >  2:2559248/1‑148 (MQ=255)
                                                                                               aCAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTg                                                >  2:162401/1‑148 (MQ=255)
                                                                                                cAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGa                                               <  2:150168/148‑1 (MQ=255)
                                                                                                  aaaaaaGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAAt                                             <  1:1461795/148‑1 (MQ=255)
                                                                                                  aaaaaaGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAAt                                             >  1:1871698/1‑148 (MQ=255)
                                                                                                   aaaaaGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAAtt                                            >  1:1944968/1‑148 (MQ=255)
                                                                                                    aaaaGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTa                                           >  2:2666484/1‑148 (MQ=255)
                                                                                                      aaGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAag                                         >  2:2172043/1‑148 (MQ=255)
                                                                                                                  aaTATGGAAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCat                             >  1:71896/1‑148 (MQ=255)
                                                                                                                    tatGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCatat                           >  2:2334266/1‑148 (MQ=255)
                                                                                                                     atGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATc                          >  1:1458861/1‑148 (MQ=255)
                                                                                                                       ggTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGa                        >  2:370472/1‑148 (MQ=255)
                                                                                                                        gTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGaa                       >  1:1140580/1‑148 (MQ=255)
                                                                                                                         tAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAg                      <  1:3192740/148‑1 (MQ=255)
                                                                                                                              tACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAgtcgtc                 <  2:2942499/148‑1 (MQ=255)
                                                                                                                                ccAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCaa               <  2:3004133/148‑1 (MQ=255)
                                                                                                                                 cAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAAc              <  1:29495/148‑1 (MQ=255)
                                                                                                                                  aGGGCAAGGTGATGGGGTAAACATTTTTTTTGTTGGTGAAATTAATGTTACGGATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTCTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACt             <  1:1813692/148‑1 (MQ=255)
                                                                                                                                    ggCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTc           <  2:1194003/148‑1 (MQ=255)
                                                                                                                                    ggCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTc           >  1:438414/1‑148 (MQ=255)
                                                                                                                                     gCACGGGGTTGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCg          <  2:452947/148‑1 (MQ=255)
                                                                                                                                       aaGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTa        <  2:1218023/148‑1 (MQ=255)
                                                                                                                                        aGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAg       <  2:327921/148‑1 (MQ=255)
                                                                                                                                        aGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAg       >  2:888790/1‑148 (MQ=255)
                                                                                                                                         ggggATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGt      <  2:1601578/148‑1 (MQ=255)
                                                                                                                                          gggATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTg     >  2:1891363/1‑148 (MQ=255)
                                                                                                                                            gATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGAg   <  2:3404018/148‑1 (MQ=255)
                                                                                                                                            gATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGAg   >  2:930393/1‑148 (MQ=255)
                                                                                                                                             aTGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGAgg  <  1:503605/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                               
CCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGAGG  >  CP009273/524351‑524639

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGAGGAAAA  >  CP009273/524347‑524643
                                                                                                                                                     |                                                                                                                                                   
TTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGG                                                                                                                                                     >  SRR19973065.2182285/1‑150 (MQ=60)
TTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGG                                                                                                                                                     >  SRR19973065.2182092/1‑150 (MQ=60)
 TTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGG                                                                                                                                                    <  SRR19973065.2535526/150‑1 (MQ=60)
   TCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTA                                                                                                                                                  <  SRR19973065.3144455/150‑1 (MQ=60)
   TCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTA                                                                                                                                                  <  SRR19973065.3144977/150‑1 (MQ=60)
    CCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAA                                                                                                                                                 <  SRR19973065.754728/150‑1 (MQ=60)
     CCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAA                                                                                                                                                >  SRR19973065.1531799/1‑150 (MQ=60)
     CCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAA                                                                                                                                                >  SRR19973065.2673062/1‑150 (MQ=60)
     CCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAA                                                                                                                                                >  SRR19973065.2769147/1‑150 (MQ=60)
     CCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAA                                                                                                                                                >  SRR19973065.3283241/1‑150 (MQ=60)
       TAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC                                                                                                                                              >  SRR19973065.13456/1‑150 (MQ=60)
           AAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTT                                                                                                                                          >  SRR19973065.2646319/1‑150 (MQ=60)
           AAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTT                                                                                                                                          <  SRR19973065.892950/150‑1 (MQ=60)
            AATGATGATGGGGGTAGGATTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTT                                                                                                                                         <  SRR19973065.2876168/150‑1 (MQ=60)
             ATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTT                                                                                                                                        >  SRR19973065.3424818/1‑150 (MQ=60)
              TGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTT                                                                                                                                       >  SRR19973065.538688/1‑150 (MQ=60)
              TGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTT                                                                                                                                       <  SRR19973065.2156464/150‑1 (MQ=60)
               GATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTT                                                                                                                                      <  SRR19973065.566458/150‑1 (MQ=60)
                  GATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTT                                                                                                                                   <  SRR19973065.2243733/150‑1 (MQ=60)
                  GATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTT                                                                                                                                   <  SRR19973065.2243612/150‑1 (MQ=60)
                  GATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTT                                                                                                                                   >  SRR19973065.1470188/1‑150 (MQ=60)
                     GGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGT                                                                                                                                <  SRR19973065.212236/150‑1 (MQ=60)
                         GTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAA                                                                                                                            <  SRR19973065.1399663/150‑1 (MQ=60)
                         GTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAA                                                                                                                            >  SRR19973065.2082935/1‑150 (MQ=60)
                           AGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATT                                                                                                                          <  SRR19973065.2217279/150‑1 (MQ=60)
                           AGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATT                                                                                                                          >  SRR19973065.2379562/1‑150 (MQ=60)
                            GGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTA                                                                                                                         <  SRR19973065.958372/150‑1 (MQ=60)
                             GTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAA                                                                                                                        >  SRR19973065.99331/1‑150 (MQ=60)
                             GTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAA                                                                                                                        <  SRR19973065.3390502/150‑1 (MQ=60)
                                TAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGT                                                                                                                     <  SRR19973065.2012324/150‑1 (MQ=60)
                                 AAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTT                                                                                                                    <  SRR19973065.1781635/150‑1 (MQ=60)
                                 AAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTT                                                                                                                    <  SRR19973065.1857781/150‑1 (MQ=60)
                                 AAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTT                                                                                                                    >  SRR19973065.2002234/1‑150 (MQ=60)
                                  AGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTA                                                                                                                   <  SRR19973065.3332966/150‑1 (MQ=60)
                                   GAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTAC                                                                                                                  <  SRR19973065.713282/150‑1 (MQ=60)
                                      AACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCA                                                                                                               >  SRR19973065.354498/1‑150 (MQ=60)
                                        CATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATT                                                                                                             >  SRR19973065.535768/1‑150 (MQ=60)
                                         ATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTA                                                                                                            <  SRR19973065.1722035/150‑1 (MQ=60)
                                          TTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTAT                                                                                                           <  SRR19973065.1166937/150‑1 (MQ=60)
                                          TTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTAT                                                                                                           <  SRR19973065.1166934/150‑1 (MQ=60)
                                          TTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTAT                                                                                                           <  SRR19973065.1166873/150‑1 (MQ=60)
                                            GACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTT                                                                                                         <  SRR19973065.1952359/150‑1 (MQ=60)
                                              CAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTA                                                                                                       <  SRR19973065.1065320/150‑1 (MQ=60)
                                               AGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTAT                                                                                                      >  SRR19973065.1471654/1‑150 (MQ=60)
                                                GTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATA                                                                                                     <  SRR19973065.1987793/150‑1 (MQ=60)
                                                 TGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAA                                                                                                    <  SRR19973065.2310301/150‑1 (MQ=60)
                                                      GCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAAT                                                                                               <  SRR19973065.2550919/150‑1 (MQ=60)
                                                      GCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAAT                                                                                               <  SRR19973065.2118401/150‑1 (MQ=60)
                                                      GCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAAT                                                                                               <  SRR19973065.939278/150‑1 (MQ=60)
                                                       CCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATA                                                                                              <  SRR19973065.1185805/150‑1 (MQ=60)
                                                       CCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATA                                                                                              >  SRR19973065.938538/1‑150 (MQ=60)
                                                       CCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATA                                                                                              <  SRR19973065.3373199/150‑1 (MQ=60)
                                                        CAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATAT                                                                                             >  SRR19973065.951281/1‑150 (MQ=60)
                                                        CAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATAT                                                                                             <  SRR19973065.3274488/150‑1 (MQ=60)
                                                         AGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATT                                                                                            <  SRR19973065.2446744/150‑1 (MQ=60)
                                                         AGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATT                                                                                            <  SRR19973065.242366/150‑1 (MQ=60)
                                                           GGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGT                                                                                          >  SRR19973065.3490385/1‑150 (MQ=60)
                                                           GGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGG                                                                                          <  SRR19973065.652624/150‑1 (MQ=60)
                                                           GGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGG                                                                                          <  SRR19973065.2425235/150‑1 (MQ=60)
                                                            GAACAAGTGGCGAAAATCGTATCAAATAATGATCCAGATACAAAAAAAGTGTGGTGTAAATAAGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATTTTACGCATTATATTATACCAAATATTGGA                                                                                         >  SRR19973065.1422084/1‑150 (MQ=60)
                                                              ACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGC                                                                                       >  SRR19973065.3429366/1‑150 (MQ=60)
                                                               CAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCT                                                                                      <  SRR19973065.421356/150‑1 (MQ=60)
                                                               CAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCT                                                                                      >  SRR19973065.3214635/1‑150 (MQ=60)
                                                               CAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCT                                                                                      <  SRR19973065.2027163/150‑1 (MQ=60)
                                                               CAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCT                                                                                      >  SRR19973065.464168/1‑150 (MQ=60)
                                                                AAGTGGCGTTAATCGTATCAAAGTATGATCCAGATACAAAAAATGTGTGGTGTAAATATGGTAAGAGACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTTATGTTACGCATTATTTTATAACAAATATTGGAGCTG                                                                                     <  SRR19973065.1752422/150‑1 (MQ=60)
                                                                   TGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGAT                                                                                  >  SRR19973065.1674193/1‑150 (MQ=60)
                                                                   TGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGAT                                                                                  >  SRR19973065.2805986/1‑150 (MQ=60)
                                                                   TGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGAT                                                                                  >  SRR19973065.931213/1‑150 (MQ=60)
                                                                    GGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATT                                                                                 >  SRR19973065.3373256/1‑150 (MQ=60)
                                                                    GGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATT                                                                                 <  SRR19973065.728654/150‑1 (MQ=60)
                                                                    GGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATT                                                                                 <  SRR19973065.728671/150‑1 (MQ=60)
                                                                      CGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGC                                                                               >  SRR19973065.2183670/1‑150 (MQ=60)
                                                                      CGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGC                                                                               >  SRR19973065.62642/1‑150 (MQ=60)
                                                                        AAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCT                                                                             <  SRR19973065.3444496/150‑1 (MQ=60)
                                                                        AAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCT                                                                             <  SRR19973065.2198650/150‑1 (MQ=60)
                                                                        AAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCT                                                                             <  SRR19973065.2531718/150‑1 (MQ=60)
                                                                         AAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTG                                                                            >  SRR19973065.151492/1‑150 (MQ=60)
                                                                         AAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTG                                                                            >  SRR19973065.151431/1‑150 (MQ=60)
                                                                         AAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTG                                                                            >  SRR19973065.984343/1‑150 (MQ=60)
                                                                           ATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGAT                                                                          >  SRR19973065.2937178/1‑150 (MQ=60)
                                                                                ATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTG                                                                     <  SRR19973065.1994302/150‑1 (MQ=60)
                                                                                ATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTG                                                                     <  SRR19973065.1995076/150‑1 (MQ=60)
                                                                                  CAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTG                                                                   >  SRR19973065.1417530/1‑150 (MQ=60)
                                                                                   AAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGC                                                                  >  SRR19973065.2327202/1‑150 (MQ=60)
                                                                                    AAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCA                                                                 >  SRR19973065.558298/1‑150 (MQ=60)
                                                                                       AATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAG                                                              >  SRR19973065.2915953/1‑150 (MQ=60)
                                                                                        ATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGT                                                             >  SRR19973065.696702/1‑150 (MQ=60)
                                                                                        ATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGT                                                             >  SRR19973065.2232341/1‑150 (MQ=60)
                                                                                         TGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTA                                                            <  SRR19973065.2643279/150‑1 (MQ=60)
                                                                                         TGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTA                                                            <  SRR19973065.2052708/150‑1 (MQ=60)
                                                                                         TGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTA                                                            <  SRR19973065.874241/150‑1 (MQ=60)
                                                                                          GATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAA                                                           >  SRR19973065.2960366/1‑150 (MQ=60)
                                                                                           ATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAAT                                                          <  SRR19973065.676208/150‑1 (MQ=60)
                                                                                                GATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTT                                                     >  SRR19973065.2338879/1‑150 (MQ=60)
                                                                                                 ATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTG                                                    <  SRR19973065.1477315/150‑1 (MQ=60)
                                                                                                 ATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTG                                                    >  SRR19973065.3493208/1‑150 (MQ=60)
                                                                                                    CAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAAT                                                 >  SRR19973065.1884027/1‑150 (MQ=60)
                                                                                                    CAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAAT                                                 >  SRR19973065.3308216/1‑150 (MQ=60)
                                                                                                     AAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATT                                                <  SRR19973065.2763330/150‑1 (MQ=60)
                                                                                                     AAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATT                                                <  SRR19973065.2156533/150‑1 (MQ=60)
                                                                                                     AAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATT                                                >  SRR19973065.1957683/1‑150 (MQ=60)
                                                                                                      AAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTA                                               <  SRR19973065.1471189/150‑1 (MQ=60)
                                                                                                         AAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGA                                            >  SRR19973065.3388521/1‑150 (MQ=60)
                                                                                                         AAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGA                                            >  SRR19973065.1342917/1‑150 (MQ=60)
                                                                                                              GTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTA                                       <  SRR19973065.2336886/150‑1 (MQ=60)
                                                                                                                GGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATC                                     <  SRR19973065.3377318/150‑1 (MQ=60)
                                                                                                                GGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATC                                     <  SRR19973065.3086614/150‑1 (MQ=60)
                                                                                                                    TAAATATGGAAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCAT                                 >  SRR19973065.72645/1‑150 (MQ=60)
                                                                                                                       ATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATC                              >  SRR19973065.1468063/1‑150 (MQ=60)
                                                                                                                       ATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATC                              >  SRR19973065.1932114/1‑150 (MQ=60)
                                                                                                                       ATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATC                              >  SRR19973065.1468079/1‑150 (MQ=60)
                                                                                                                       ATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATC                              >  SRR19973065.1468244/1‑150 (MQ=60)
                                                                                                                          TGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAA                           >  SRR19973065.2136299/1‑150 (MQ=60)
                                                                                                                          TGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAA                           >  SRR19973065.1147956/1‑150 (MQ=60)
                                                                                                                            GTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGT                         >  SRR19973065.3400898/1‑150 (MQ=60)
                                                                                                                             TAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTC                        <  SRR19973065.3211996/150‑1 (MQ=60)
                                                                                                                                GATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTC                     >  SRR19973065.2775225/1‑150 (MQ=60)
                                                                                                                                   ACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAAC                  >  SRR19973065.2718886/1‑150 (MQ=60)
                                                                                                                                    CCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACT                 <  SRR19973065.1848708/150‑1 (MQ=60)
                                                                                                                                     CAGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTT                <  SRR19973065.29876/150‑1 (MQ=60)
                                                                                                                                      AGGGCAAGGTGATGGGGTAAACATTTTTTTTGTTGGTGAAATTAATGTTACGGATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTCTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTC               <  SRR19973065.1825634/150‑1 (MQ=60)
                                                                                                                                      AGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTC               >  SRR19973065.441452/1‑150 (MQ=60)
                                                                                                                                      AGGGCAAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTC               >  SRR19973065.2583485/1‑150 (MQ=60)
                                                                                                                                           AAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGT          <  SRR19973065.748679/150‑1 (MQ=60)
                                                                                                                                           AAGGGGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGT          >  SRR19973065.1123682/1‑150 (MQ=60)
                                                                                                                                               GGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGAGG      <  SRR19973065.1877347/150‑1 (MQ=60)
                                                                                                                                               GGATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGAGG      <  SRR19973065.1602106/150‑1 (MQ=60)
                                                                                                                                                 ATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGAGGAA    <  SRR19973065.506967/150‑1 (MQ=60)
                                                                                                                                                 ATGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGAGGAA    >  SRR19973065.1540814/1‑150 (MQ=60)
                                                                                                                                                  TGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGAGGAAA   >  SRR19973065.1590847/1‑150 (MQ=60)
                                                                                                                                                  TGGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGAGGAAA   >  SRR19973065.1590823/1‑150 (MQ=60)
                                                                                                                                                   GGGGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGAGGAAAA  >  SRR19973065.1568827/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                   
TTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAAACCTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGAGGAAAA  >  CP009273/524347‑524643

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑