Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 524,496 T→G 100% pseudogene (401/491 nt) ybbD → pseudogene

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273524,4960TG97.0% 726.9 / 3.7 197pseudogene (401/491 nt)ybbDpseudogene
Reads supporting (aligned to +/- strand):  ref base T (4/2);  new base G (104/86);  total (109/88)
Fisher's exact test for biased strand distribution p-value = 6.93e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.01e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 201 total aligned reads displayed.

TTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGTGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGAGGAAA  >  CP009273/524349‑524642
                                                                                                                                                    |                                                                                                                                                   
ttCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGgggt                                                                                                                                                   <  1:4556206/150‑1 (MQ=255)
ttCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGgggt                                                                                                                                                   <  1:1155453/150‑1 (MQ=255)
ttCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGgggt                                                                                                                                                   >  1:1874528/1‑150 (MQ=255)
 tCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTa                                                                                                                                                  <  2:1181810/150‑1 (MQ=255)
  cccTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTaa                                                                                                                                                 >  1:4088854/1‑150 (MQ=255)
  cccTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTaa                                                                                                                                                 >  2:255455/1‑150 (MQ=255)
     tAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAAcc                                                                                                                                              <  2:3012668/150‑1 (MQ=255)
       aCAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑tt                                                                                                                                           >  2:4738083/1‑150 (MQ=255)
       aCAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACA‑tt                                                                                                                                           >  1:3200651/1‑150 (MQ=255)
        cAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑ttt                                                                                                                                          <  2:406179/150‑1 (MQ=255)
        cAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑ttt                                                                                                                                          >  1:52832/1‑150 (MQ=255)
        cAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑ttt                                                                                                                                          <  2:468583/150‑1 (MQ=255)
             gatgatgGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑tttttttt                                                                                                                                     >  1:3048600/1‑150 (MQ=255)
               tgatgGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTttgt                                                                                                                                   >  2:3662964/1‑150 (MQ=255)
                 atgGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTttgttg                                                                                                                                 >  2:859632/1‑150 (MQ=255)
                    ggggTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAGAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTg                                                                                                                              <  2:1156187/150‑1 (MQ=255)
                      ggTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGaa                                                                                                                            <  2:3562723/150‑1 (MQ=255)
                      ggTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGaa                                                                                                                            >  1:2400459/1‑150 (MQ=255)
                         aGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAAtt                                                                                                                         <  2:3255598/150‑1 (MQ=255)
                         aGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAAtt                                                                                                                         <  1:2916741/150‑1 (MQ=255)
                           gTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTaa                                                                                                                       <  1:1377723/150‑1 (MQ=255)
                            tttAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAAt                                                                                                                      <  1:3746598/150‑1 (MQ=255)
                             tnAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATg                                                                                                                     >  1:1931417/1‑150 (MQ=255)
                               aaGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGtt                                                                                                                   >  1:79223/1‑150 (MQ=255)
                                aGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTa                                                                                                                  >  2:4015608/1‑150 (MQ=255)
                                aGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTa                                                                                                                  >  2:1437677/1‑150 (MQ=255)
                                   aaaCATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGc                                                                                                               <  2:200484/150‑1 (MQ=255)
                                    aaCATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCa                                                                                                              >  2:253185/1‑150 (MQ=255)
                                        ttGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCattat                                                                                                          >  1:1011627/1‑150 (MQ=255)
                                          gACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTAttt                                                                                                        >  1:4246056/1‑150 (MQ=255)
                                            cAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTta                                                                                                      >  2:2364869/1‑150 (MQ=255)
                                                gAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAAc                                                                                                  <  2:3418390/150‑1 (MQ=255)
                                                      cAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGTGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAatat                                                                                            >  1:1781019/1‑150 (MQ=255)
                                                      cAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAatat                                                                                            >  1:4100459/1‑150 (MQ=255)
                                                       aGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATAtt                                                                                           >  2:4571141/1‑150 (MQ=255)
                                                          gAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGa                                                                                        >  2:4081732/1‑150 (MQ=255)
                                                          gAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATAC‑AAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAg                                                                                       >  2:276885/1‑150 (MQ=255)
                                                            aCAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGc                                                                                      >  2:1902997/1‑150 (MQ=255)
                                                              aaGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTg                                                                                    <  1:256604/150‑1 (MQ=255)
                                                                gTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGa                                                                                  >  1:659762/1‑150 (MQ=255)
                                                                 tGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGAt                                                                                 <  2:1516078/150‑1 (MQ=255)
                                                                 tGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGAt                                                                                 <  2:2133113/150‑1 (MQ=255)
                                                                   gCGAAAATCGTATCAAAGAATGATACAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTATTAGTTGGTGAAATTAATGTTACGCATAATGTTATAACAAATATATGAGCTGGATTg                                                                               >  1:1550054/1‑150 (MQ=255)
                                                                      aaaaTCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCt                                                                            >  1:2066539/1‑150 (MQ=255)
                                                                       aaaTCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTg                                                                           >  2:382833/1‑150 (MQ=255)
                                                                       aaaTCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTg                                                                           <  2:1954837/150‑1 (MQ=255)
                                                                       aaaTCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTg                                                                           <  1:859632/150‑1 (MQ=255)
                                                                        aaTCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACCTTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTg                                                                           >  1:1344405/1‑150 (MQ=255)
                                                                           cGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGc                                                                       <  2:1954986/150‑1 (MQ=255)
                                                                            gTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCt                                                                      <  1:1429618/150‑1 (MQ=255)
                                                                             tATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCtt                                                                     <  2:2850982/150‑1 (MQ=255)
                                                                              aTCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTtg                                                                    <  1:158304/150‑1 (MQ=255)
                                                                                   aGAATGATCCAGATACAAAAAAAGTGTGGTGTATATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCag                                                               <  2:4325868/150‑1 (MQ=255)
                                                                                   aGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCag                                                               >  2:4688635/1‑150 (MQ=255)
                                                                                   aGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCag                                                               >  1:107029/1‑150 (MQ=255)
                                                                                     aaTGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCagag                                                             >  1:1085974/1‑150 (MQ=255)
                                                                                     aaTGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCagag                                                             >  1:4041571/1‑150 (MQ=255)
                                                                                      aTGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGt                                                            >  1:3903648/1‑150 (MQ=255)
                                                                                       tGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAAATAATGTTACGCATTATTTTATAACAAATTTTGGAGCGGGATTGGCTGATGCTTGTGCAGAGTa                                                           >  2:4877380/1‑150 (MQ=255)
                                                                                         aTCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAAt                                                         >  1:887043/1‑150 (MQ=255)
                                                                                         aTCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAAt                                                         >  1:3567311/1‑150 (MQ=255)
                                                                                          tCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGAGGGGGTAACCC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAAtt                                                        <  2:1542219/150‑1 (MQ=255)
                                                                                           ccAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTg                                                       <  2:4173092/150‑1 (MQ=255)
                                                                                             aGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGca                                                     <  2:4434710/150‑2 (MQ=255)
                                                                                             aGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCt                                                     >  2:455240/1‑150 (MQ=255)
                                                                                                tACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGa                                                  <  1:961212/150‑1 (MQ=255)
                                                                                                tACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGa                                                  >  1:1704050/1‑150 (MQ=255)
                                                                                                   aaaaaaaGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAAtt                                               >  2:3719284/1‑150 (MQ=255)
                                                                                                    aaaaaaGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGTGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTa                                              >  1:1712076/1‑150 (MQ=255)
                                                                                                      caaaGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTCATTGCTTGAATTAag                                            >  2:1413196/2‑150 (MQ=255)
                                                                                                      aaaaGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAag                                            >  1:1242901/1‑150 (MQ=255)
                                                                                                         aGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGt                                         >  2:1816476/1‑150 (MQ=255)
                                                                                                             tggtgTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTAt                                     <  1:3793583/150‑1 (MQ=255)
                                                                                                             tggtgTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTAt                                     <  1:3402347/150‑1 (MQ=255)
                                                                                                               gtgtAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATcc                                   <  2:4623759/150‑1 (MQ=255)
                                                                                                               gtgtAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATcc                                   >  2:3180396/1‑150 (MQ=255)
                                                                                                                 gtAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCa                                 <  1:5088174/150‑1 (MQ=255)
                                                                                                                 gtAAATATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCa                                 <  1:3652328/150‑1 (MQ=255)
                                                                                                                   aaaTATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCata                               >  2:4542150/1‑150 (MQ=255)
                                                                                                                   aaaTATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCata                               >  1:4413584/1‑150 (MQ=255)
                                                                                                                   aaaTATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCata                               >  1:2286280/1‑150 (MQ=255)
                                                                                                                    aaTATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCatat                              >  2:4482926/1‑150 (MQ=255)
                                                                                                                    aaTATGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCatat                              <  2:447006/150‑1 (MQ=255)
                                                                                                                      tatGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCataaaa                            >  2:114421/1‑147 (MQ=255)
                                                                                                                      tatGGTAAGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCg                            <  2:695358/150‑1 (MQ=255)
                                                                                                                           tAAGATACCAGGGCAA‑GGGGATGGGGTAAACCTTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAgt                        >  1:173985/1‑150 (MQ=255)
                                                                                                                            aaGATACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAgtcg                      <  2:3602865/150‑1 (MQ=255)
                                                                                                                                tACCAGGGCAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCaa                  <  2:2908369/150‑1 (MQ=255)
                                                                                                                                   cAGGGCAATGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACt                >  2:1265412/1‑150 (MQ=255)
                                                                                                                                    aGGTCAA‑GGTGATGGGGTAAACA‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTc              <  2:5067427/150‑1 (MQ=255)
                                                                                                                                        cAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAg          <  2:5116790/150‑1 (MQ=255)
                                                                                                                                        cAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAg          >  1:4443352/1‑150 (MQ=255)
                                                                                                                                        cAA‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAg          <  2:322669/150‑1 (MQ=255)
                                                                                                                                         aa‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGt         >  1:3221604/1‑150 (MQ=255)
                                                                                                                                          a‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTg        <  1:2688810/150‑1 (MQ=255)
                                                                                                                                          a‑GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTg        <  1:3664317/150‑1 (MQ=255)
                                                                                                                                            ggggATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGa       >  2:2468730/1‑150 (MQ=255)
                                                                                                                                               gATGGGGTAAACCTTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGAgg     <  1:5014231/150‑1 (MQ=255)
                                                                                                                                               gATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGAGGa    >  2:1782895/1‑150 (MQ=255)
                                                                                                                                                 tGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGAGGaaa  <  2:3204505/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                   
TTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAA‑GGGGATGGTGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGAGGAAA  >  CP009273/524349‑524642

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGAGGAAAAGT  >  CP009273/524347‑524645
                                                                                                                                                     |                                                                                                                                                      
TTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGG                                                                                                                                                        <  SRR19973063.4455622/150‑1 (MQ=60)
  TTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGT                                                                                                                                                      >  SRR19973063.1083889/1‑150 (MQ=60)
  TTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGT                                                                                                                                                      >  SRR19973063.1880388/1‑150 (MQ=60)
  TTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGT                                                                                                                                                      <  SRR19973063.1158940/150‑1 (MQ=60)
  TTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGT                                                                                                                                                      <  SRR19973063.4570804/150‑1 (MQ=60)
    CCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAA                                                                                                                                                    >  SRR19973063.2666267/1‑150 (MQ=60)
    CCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAA                                                                                                                                                    >  SRR19973063.4101957/1‑150 (MQ=60)
     CCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAA                                                                                                                                                   >  SRR19973063.697401/1‑150 (MQ=60)
      CTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAAC                                                                                                                                                  <  SRR19973063.4020260/150‑1 (MQ=60)
       TAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC                                                                                                                                                 >  SRR19973063.3214810/1‑150 (MQ=60)
         ACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACA‑TT                                                                                                                                              >  SRR19973063.3210940/1‑150 (MQ=60)
          CAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTT                                                                                                                                             >  SRR19973063.53020/1‑150 (MQ=60)
            AATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTT                                                                                                                                           <  SRR19973063.3718906/150‑1 (MQ=60)
             ATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGTCAAGGGGATGGGGTAAACC‑TTTTTT                                                                                                                                          >  SRR19973063.3492445/1‑150 (MQ=60)
             ATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTT                                                                                                                                          >  SRR19973063.1597780/1‑150 (MQ=60)
               GATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTT                                                                                                                                        >  SRR19973063.3058355/1‑150 (MQ=60)
                   ATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGG‑GTAAACC‑TTTTTTTTGTTGG                                                                                                                                   >  SRR19973063.1069913/1‑150 (MQ=60)
                        GGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAA                                                                                                                               >  SRR19973063.2408138/1‑150 (MQ=60)
                           AGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCCAGGGGATGGTGTAAACC‑TTTTTTTTGTTGGTGAAATT                                                                                                                            <  SRR19973063.1124196/150‑1 (MQ=60)
                           AGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATT                                                                                                                            <  SRR19973063.2926044/150‑1 (MQ=60)
                             GTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAA                                                                                                                          <  SRR19973063.1381878/150‑1 (MQ=60)
                             GTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAA                                                                                                                          <  SRR19973063.1381874/150‑1 (MQ=60)
                              TTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAAT                                                                                                                         <  SRR19973063.3758569/150‑1 (MQ=60)
                               TNAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATG                                                                                                                        >  SRR19973063.1937455/1‑150 (MQ=60)
                                 AAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTT                                                                                                                      >  SRR19973063.79494/1‑150 (MQ=60)
                                     AAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGC                                                                                                                  >  SRR19973063.1270247/1‑150 (MQ=60)
                                          TTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTAT                                                                                                             >  SRR19973063.1014678/1‑150 (MQ=60)
                                            GACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTT                                                                                                           >  SRR19973063.4259588/1‑150 (MQ=60)
                                              CAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTA                                                                                                         >  SRR19973063.2801071/1‑150 (MQ=60)
                                              CAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTA                                                                                                         >  SRR19973063.1890606/1‑150 (MQ=60)
                                                 TGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAA                                                                                                      >  SRR19973063.2355703/1‑150 (MQ=60)
                                                        CAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATAT                                                                                               >  SRR19973063.1786516/1‑150 (MQ=60)
                                                        CAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATAT                                                                                               >  SRR19973063.1786465/1‑150 (MQ=60)
                                                        CAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATAT                                                                                               >  SRR19973063.4113600/1‑150 (MQ=60)
                                                        CAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATAT                                                                                               >  SRR19973063.4113518/1‑150 (MQ=60)
                                                               CAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAAGTAATGTTACGCATTATTTTATAACAAATATTGGAGCT                                                                                        >  SRR19973063.2495279/1‑150 (MQ=60)
                                                                AAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTG                                                                                       <  SRR19973063.257373/150‑1 (MQ=60)
                                                                  GTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAACTAGTGGAGCTGGA                                                                                     >  SRR19973063.661693/1‑150 (MQ=60)
                                                                    GGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATT                                                                                   <  SRR19973063.4116419/150‑1 (MQ=60)
                                                                     GCGAAAATCGTATCAAAGAATGATACAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTATTAGTTGGTGAAATTAATGTTACGCATAATGTTATAACAAATATATGAGCTGGATTG                                                                                  >  SRR19973063.1554803/1‑150 (MQ=60)
                                                                       GAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCC                                                                                >  SRR19973063.890728/1‑150 (MQ=60)
                                                                        AAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCT                                                                               <  SRR19973063.3807153/150‑1 (MQ=60)
                                                                        AAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCT                                                                               >  SRR19973063.2072999/1‑150 (MQ=60)
                                                                         AAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTG                                                                              <  SRR19973063.862105/150‑1 (MQ=60)
                                                                          AATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTG                                                                              >  SRR19973063.1348443/1‑150 (MQ=60)
                                                                            TCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATG                                                                           >  SRR19973063.864093/1‑150 (MQ=60)
                                                                              GTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCT                                                                         <  SRR19973063.1433975/150‑1 (MQ=60)
                                                                              GTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCT                                                                         <  SRR19973063.1433980/150‑1 (MQ=60)
                                                                                ATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTG                                                                       <  SRR19973063.158736/150‑1 (MQ=60)
                                                                                ATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTG                                                                       <  SRR19973063.158797/150‑1 (MQ=60)
                                                                                 TCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGT                                                                      <  SRR19973063.3203452/150‑1 (MQ=60)
                                                                                     AGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAG                                                                  >  SRR19973063.521177/1‑150 (MQ=60)
                                                                                     AGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAG                                                                  >  SRR19973063.107390/1‑150 (MQ=60)
                                                                                       AATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAG                                                                >  SRR19973063.4054554/1‑150 (MQ=60)
                                                                                       AATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAG                                                                >  SRR19973063.4054524/1‑150 (MQ=60)
                                                                                       AATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAG                                                                >  SRR19973063.1089281/1‑150 (MQ=60)
                                                                                        ATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGT                                                               >  SRR19973063.3916084/1‑150 (MQ=60)
                                                                                         TGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTA                                                              >  SRR19973063.1403459/1‑150 (MQ=60)
                                                                                           ATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAAT                                                            >  SRR19973063.3578748/1‑150 (MQ=60)
                                                                                           ATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAAT                                                            >  SRR19973063.889608/1‑150 (MQ=60)
                                                                                            TCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATT                                                           <  SRR19973063.1725177/150‑1 (MQ=60)
                                                                                             CCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTG                                                          >  SRR19973063.2205617/1‑150 (MQ=60)
                                                                                                  TACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGA                                                     <  SRR19973063.964105/150‑1 (MQ=60)
                                                                                                  TACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGA                                                     >  SRR19973063.1709244/1‑150 (MQ=60)
                                                                                                   ACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAA                                                    >  SRR19973063.361489/1‑150 (MQ=60)
                                                                                                      AAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGTGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTA                                                 >  SRR19973063.1716907/1‑150 (MQ=60)
                                                                                                      AAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGTGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTA                                                 >  SRR19973063.1717296/1‑150 (MQ=60)
                                                                                                        AAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAG                                               >  SRR19973063.1246610/1‑150 (MQ=60)
                                                                                                           AGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGT                                            >  SRR19973063.621713/1‑150 (MQ=60)
                                                                                                               TGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTAT                                        <  SRR19973063.3805664/150‑1 (MQ=60)
                                                                                                               TGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTAT                                        <  SRR19973063.3805677/150‑1 (MQ=60)
                                                                                                               TGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTAT                                        <  SRR19973063.3413169/150‑1 (MQ=60)
                                                                                                                   GTAAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCA                                    <  SRR19973063.5104552/150‑1 (MQ=60)
                                                                                                                   GTAAATATGGTAAGAGACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCA                                    <  SRR19973063.3664040/150‑1 (MQ=60)
                                                                                                                     AAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATA                                  >  SRR19973063.4427743/1‑150 (MQ=60)
                                                                                                                     AAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATA                                  >  SRR19973063.4427793/1‑150 (MQ=60)
                                                                                                                     AAATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATA                                  >  SRR19973063.2293576/1‑150 (MQ=60)
                                                                                                                       ATATGGTAAGATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATC                                >  SRR19973063.3354099/1‑150 (MQ=60)
                                                                                                                             TAAGATACCAGGGCAAGGGGATGGGGTAAACCTTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGT                           >  SRR19973063.174524/1‑150 (MQ=60)
                                                                                                                                 ATACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCA                      <  SRR19973063.4753178/150‑1 (MQ=60)
                                                                                                                                  TACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAA                     <  SRR19973063.3674705/150‑1 (MQ=60)
                                                                                                                                   ACCAGGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAAC                    >  SRR19973063.1879762/1‑150 (MQ=60)
                                                                                                                                       TGGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCG                >  SRR19973063.3048637/1‑150 (MQ=60)
                                                                                                                                        GGCAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGT               >  SRR19973063.859345/1‑150 (MQ=60)
                                                                                                                                          CAAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAG             >  SRR19973063.4457606/1‑150 (MQ=60)
                                                                                                                                           AAGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGT            >  SRR19973063.3231961/1‑150 (MQ=60)
                                                                                                                                            AGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTTAAATTATTGTTACGCATTATTTTATAACAAATATTGGAGCTGTATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGTAGTCGTCAACTTCGTAGTG           <  SRR19973063.4565520/150‑1 (MQ=60)
                                                                                                                                            AGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTG           <  SRR19973063.2697471/150‑1 (MQ=60)
                                                                                                                                            AGGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTG           <  SRR19973063.3676060/150‑1 (MQ=60)
                                                                                                                                             GGGGATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGA          <  SRR19973063.1964429/150‑1 (MQ=60)
                                                                                                                                                GATGGGGTAAACCTTTTTTTTTGTTGGTGAAATTAATGTTTCGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGAGG        <  SRR19973063.5030674/150‑1 (MQ=60)
                                                                                                                                                GATGGGGTAAACCTTTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGAGG        <  SRR19973063.5030419/150‑1 (MQ=60)
                                                                                                                                                 ATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGAGGAA      <  SRR19973063.4367381/150‑1 (MQ=60)
                                                                                                                                                 ATGGGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGAGGAA      <  SRR19973063.1169369/150‑1 (MQ=60)
                                                                                                                                                    GGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGAGGAAAAG   <  SRR19973063.936288/150‑1 (MQ=60)
                                                                                                                                                    GGGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGAGGAAAAG   <  SRR19973063.952712/150‑1 (MQ=60)
                                                                                                                                                     GGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGAGGAAAAGT  <  SRR19973063.3435376/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                      
TTTTCCCTAACAAATGATGATGGGGGTAGGTTTAAGAAAACATTGACAGTGAGGGCCAGGGAACAAGTGGCGAAAATCGTATCAAAGAATGATCCAGATACAAAAAAAGTGTGGTGTAAATATGGTAAGATACCAGGGCAAGGGGATGGTGTAAACC‑TTTTTTTTGTTGGTGAAATTAATGTTACGCATTATTTTATAACAAATATTGGAGCTGGATTGCCTGATGCTTGTGCAGAGTAATTGCTTGAATTAAGAGTCTATCCCATATCGAAGTCGTCAACTTCGTAGTGAGGAAAAGT  >  CP009273/524347‑524645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑