Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F434 I0 R1 1130 215.9 7031662 97.1% 6827743 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,409,856 A→C 100% T489P (ACC→CCC)  acrF → multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,409,8560AC97.8% 879.9 / 0.7 227T489P (ACC→CCC) acrFmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base A (4/1);  new base C (118/104);  total (122/105)
Fisher's exact test for biased strand distribution p-value = 3.77e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.36e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 229 total aligned reads displayed.

GCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCA  >  CP009273/3409712‑3410000
                                                                                                                                                |                                                                                                                                                
  aCTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCt                                                                                                                                             >  2:1218170/1‑148 (MQ=255)
    tggtggGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGc                                                                                                                                           <  2:1235224/148‑1 (MQ=255)
     ggtggGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCg                                                                                                                                          >  1:524859/1‑148 (MQ=255)
     ggtggGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCg                                                                                                                                          <  1:1412524/148‑1 (MQ=255)
     ggtggGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCg                                                                                                                                          <  1:1731932/148‑1 (MQ=255)
      gtggGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGt                                                                                                                                         >  2:1428591/1‑148 (MQ=255)
         ggTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTAt                                                                                                                                      >  1:335038/1‑148 (MQ=255)
         ggTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTAt                                                                                                                                      <  2:212081/148‑1 (MQ=255)
          gTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTAtg                                                                                                                                     <  1:626666/148‑1 (MQ=255)
          gTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTAtg                                                                                                                                     <  2:2540347/148‑1 (MQ=255)
          gTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTAtg                                                                                                                                     <  2:2540340/148‑1 (MQ=255)
          gTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTACCCCTGCGTTa                                                                                                                                       <  1:1517502/146‑1 (MQ=255)
              cgcgATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCa                                                                                                                                 >  1:1682954/1‑148 (MQ=255)
               gcgATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCaa                                                                                                                                <  1:874547/148‑1 (MQ=255)
                 gATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACg                                                                                                                              <  1:2900400/148‑1 (MQ=255)
                   tGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACgct                                                                                                                            >  2:724767/1‑148 (MQ=255)
                   tGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACgct                                                                                                                            >  2:724771/1‑148 (MQ=255)
                       gCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCtt                                                                                                                        >  2:3284520/1‑148 (MQ=255)
                         tGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTaa                                                                                                                      <  2:2702316/148‑1 (MQ=255)
                         tGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTaa                                                                                                                      >  1:3455517/1‑148 (MQ=255)
                          gTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTaaa                                                                                                                     >  1:823915/1‑148 (MQ=255)
                            cAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAAcc                                                                                                                   >  2:2877313/1‑148 (MQ=255)
                            cAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAAcc                                                                                                                   >  1:401519/1‑148 (MQ=255)
                                  tatttattCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGtctc                                                                                                             >  1:693488/1‑148 (MQ=255)
                                  tatttattCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGtctc                                                                                                             >  1:373956/1‑148 (MQ=255)
                                  tatttattCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACACGtctc                                                                                                             >  1:373996/1‑148 (MQ=255)
                                    tttattCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTctg                                                                                                           <  2:956601/148‑1 (MQ=255)
                                    tttattCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTctg                                                                                                           >  1:2158800/1‑148 (MQ=255)
                                     ttattCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTctgc                                                                                                          <  1:3212076/148‑1 (MQ=255)
                                      tattCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTctgct                                                                                                         >  2:2647294/1‑148 (MQ=255)
                                      tattCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTctgct                                                                                                         >  1:2236289/1‑148 (MQ=255)
                                         tCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAg                                                                                                      >  2:2958348/1‑148 (MQ=255)
                                         tCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAg                                                                                                      >  2:3081699/1‑148 (MQ=255)
                                            gATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCAt                                                                                                   >  2:1469448/1‑148 (MQ=255)
                                            gATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCAt                                                                                                   <  1:395544/148‑1 (MQ=255)
                                             aTGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATc                                                                                                  <  1:1088128/148‑1 (MQ=255)
                                               ggCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCGGAGCATCAc                                                                                                >  1:993485/1‑148 (MQ=255)
                                                 cATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGa                                                                                              >  2:674797/1‑148 (MQ=255)
                                                 cATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTGTCGGCAATGGCGCTGTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGa                                                                                              >  2:2726017/1‑148 (MQ=255)
                                                    tcttcGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGaaaa                                                                                           >  2:44757/1‑148 (MQ=255)
                                                     cttcGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTATTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAAt                                                                                          >  1:689725/1‑148 (MQ=255)
                                                       tcGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATaa                                                                                        <  1:2359762/148‑1 (MQ=255)
                                                       tcGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATaa                                                                                        <  1:2421898/148‑1 (MQ=255)
                                                              ttCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGt                                                                                 >  2:1237155/1‑148 (MQ=255)
                                                              ttCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGt                                                                                 >  2:3272712/1‑148 (MQ=255)
                                                              ttCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGt                                                                                 >  2:3467150/1‑148 (MQ=255)
                                                                      gggCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCgg                                                                         >  2:2334639/1‑148 (MQ=255)
                                                                       ggCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCggt                                                                        >  1:1614797/1‑148 (MQ=255)
                                                                         cAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCggttg                                                                      <  2:49522/148‑1 (MQ=255)
                                                                         cAATTTATCGCCAGTTCTCGATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCggttg                                                                      <  2:1827198/148‑1 (MQ=255)
                                                                         cAACTTAGCGCCAGTACTCCATCCCCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCggttg                                                                      <  1:2060853/148‑1 (MQ=255)
                                                                          aaTTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCggttgg                                                                     <  1:349124/148‑1 (MQ=255)
                                                                          aaTTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCggttgg                                                                     <  2:2196583/148‑1 (MQ=255)
                                                                           aTTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCggttggt                                                                    <  2:262646/148‑1 (MQ=255)
                                                                           aTTTATCGCCAGTTCTCTATCACCATCGTTTAGGCAATGGCGCTTTCTGTTCTGGTGGCATTGTTTCTTCACCCTGCGTTATGTGCATCGCTGCTTTAACCCTTCTTTGCTGAGGATCTGTTTAATAAGGGCGGTTTCTTCggttggt                                                                    <  1:508666/148‑1 (MQ=255)
                                                                            tttATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCggttggtt                                                                   >  1:1937621/1‑148 (MQ=255)
                                                                            tttATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCggttggtt                                                                   >  1:1939141/1‑148 (MQ=255)
                                                                            tttATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCggttggtt                                                                   <  2:827090/148‑1 (MQ=255)
                                                                             ttATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGttt                                                                  >  1:3164720/1‑148 (MQ=255)
                                                                                tCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAAt                                                               >  1:1420757/1‑148 (MQ=255)
                                                                                 cGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATa                                                              <  1:1013674/148‑1 (MQ=255)
                                                                                 cGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGAATCTGAGTCGCAAAACGAAAATAAGGGCGGTTACTGCGGTTGGTTTAATa                                                              >  2:2879434/1‑148 (MQ=255)
                                                                                   ccAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATacc                                                            <  1:784776/148‑1 (MQ=255)
                                                                                    cAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATacca                                                           <  2:3512528/148‑1 (MQ=255)
                                                                                    cAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCGTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATacca                                                           <  2:3512910/148‑1 (MQ=255)
                                                                                      gTTCTCTATCACCATCTTTTCGGCAATGGCGCTTTCTGTTCTGGTGCCAATGATTAATCACCCTGCGTTATGTGCAAAGCTGCTTAAACCCGTCTATGCTGAGAATCACTAAAATAAGGGCGGTTTCTTCGGTTTGTTTAATaacaaa                                                         >  2:2899972/1‑146 (MQ=255)
                                                                                      gTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATaccacc                                                         <  1:2444516/148‑1 (MQ=255)
                                                                                       ttCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCt                                                        >  1:2350006/1‑148 (MQ=255)
                                                                                        tctcTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCGTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCtt                                                       <  1:1395689/148‑1 (MQ=255)
                                                                                         ctctATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTc                                                      <  1:2846614/148‑1 (MQ=255)
                                                                                           ctATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTATTCGGTTGGTTTAATACCACCTTCGa                                                    >  1:2413113/1‑148 (MQ=255)
                                                                                              tCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCa                                                 >  2:880525/1‑148 (MQ=255)
                                                                                              tCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCa                                                 <  1:1287798/148‑1 (MQ=255)
                                                                                               cACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCAt                                                <  1:3082760/148‑1 (MQ=255)
                                                                                               cACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCAt                                                >  1:1986368/1‑148 (MQ=255)
                                                                                                aCCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATa                                               >  2:2095432/1‑148 (MQ=255)
                                                                                                 ccATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAg                                              >  1:2788873/1‑148 (MQ=255)
                                                                                                   aTCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCg                                            >  2:1373898/1‑148 (MQ=255)
                                                                                                   aTCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCg                                            >  2:3120782/1‑148 (MQ=255)
                                                                                                      gTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTa                                         <  2:189142/148‑1 (MQ=255)
                                                                                                         tCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAAcc                                      <  1:2647690/148‑1 (MQ=255)
                                                                                                         tCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCGTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAAcc                                      <  1:1822781/148‑1 (MQ=255)
                                                                                                          cGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCa                                     <  2:2839547/148‑1 (MQ=255)
                                                                                                                   cgcTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCaa                            >  1:1364668/1‑148 (MQ=255)
                                                                                                                      tttCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAg                         <  1:658486/148‑1 (MQ=255)
                                                                                                                           gttctgGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCg                    >  2:1305862/1‑148 (MQ=255)
                                                                                                                             tctgGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGc                  >  1:1456404/1‑148 (MQ=255)
                                                                                                                                ggtggCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCaaa               <  1:1043228/148‑1 (MQ=255)
                                                                                                                                  tggCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAAt             >  1:1816309/1‑148 (MQ=255)
                                                                                                                                    gCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATcc           >  2:1539164/1‑148 (MQ=255)
                                                                                                                                     cATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCt          <  2:2021940/148‑1 (MQ=255)
                                                                                                                                     cATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCt          <  1:1218200/148‑1 (MQ=255)
                                                                                                                                      aTTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTc         <  2:3360674/148‑1 (MQ=255)
                                                                                                                                        tGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCgg       >  2:245668/1‑148 (MQ=255)
                                                                                                                                         gATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGa      <  2:524859/148‑1 (MQ=255)
                                                                                                                                           ttcttcCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATc    <  2:2297983/148‑1 (MQ=255)
                                                                                                                                            tCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATcc   >  2:748858/1‑148 (MQ=255)
                                                                                                                                            tCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATcc   >  2:1637820/1‑148 (MQ=255)
                                                                                                                                            tCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATcc   >  1:2728584/1‑148 (MQ=255)
                                                                                                                                             cTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCca  <  2:473254/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                
GCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCA  >  CP009273/3409712‑3410000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGGCGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCACAGG  >  CP009273/3409708‑3410004
                                                                                                                                                    |                                                                                                                                                    
GGGCGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCC                                                                                                                                                     <  SRR19973065.3501451/150‑1 (MQ=60)
GGGCGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCC                                                                                                                                                     <  SRR19973065.451687/150‑1 (MQ=60)
  GCGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCC                                                                                                                                                   <  SRR19973065.2321147/150‑1 (MQ=60)
   CGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCC                                                                                                                                                  >  SRR19973065.811277/1‑150 (MQ=60)
       CTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCG                                                                                                                                              >  SRR19973065.528308/1‑150 (MQ=60)
         GGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTT                                                                                                                                            <  SRR19973065.1421755/150‑1 (MQ=60)
         GGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTT                                                                                                                                            <  SRR19973065.1743395/150‑1 (MQ=60)
          GTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTA                                                                                                                                           <  SRR19973065.79161/150‑1 (MQ=60)
           TGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTAT                                                                                                                                          >  SRR19973065.337383/1‑150 (MQ=60)
            GGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTACCCCTGCGTTATG                                                                                                                                         <  SRR19973065.1527426/150‑1 (MQ=60)
             GGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGT                                                                                                                                        <  SRR19973065.527782/150‑1 (MQ=60)
              GTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTG                                                                                                                                       <  SRR19973065.630738/150‑1 (MQ=60)
              GTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTG                                                                                                                                       <  SRR19973065.630720/150‑1 (MQ=60)
                ATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCA                                                                                                                                     >  SRR19973065.1694211/1‑150 (MQ=60)
                ATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCA                                                                                                                                     >  SRR19973065.2034946/1‑150 (MQ=60)
                ATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCA                                                                                                                                     >  SRR19973065.1694210/1‑150 (MQ=60)
                   GCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACG                                                                                                                                  <  SRR19973065.880135/150‑1 (MQ=60)
                     GATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCT                                                                                                                                <  SRR19973065.2918092/150‑1 (MQ=60)
                     GATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCT                                                                                                                                >  SRR19973065.1325723/1‑150 (MQ=60)
                        GGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCT                                                                                                                             <  SRR19973065.2882584/150‑1 (MQ=60)
                         GTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGGTCTACTGGGGCACTTTATCGCCAGTTCTCTATCACCATCGTTTCGGCTATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTTTGTGCAACGCTGCTT                                                                                                                            <  SRR19973065.3093038/150‑1 (MQ=60)
                           GCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAA                                                                                                                          >  SRR19973065.692608/1‑150 (MQ=60)
                           GCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAA                                                                                                                          >  SRR19973065.3476579/1‑150 (MQ=60)
                            CTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAA                                                                                                                         >  SRR19973065.829321/1‑150 (MQ=60)
                            CTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAA                                                                                                                         >  SRR19973065.829320/1‑150 (MQ=60)
                              GTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACC                                                                                                                       >  SRR19973065.404333/1‑150 (MQ=60)
                              GTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACC                                                                                                                       >  SRR19973065.404102/1‑150 (MQ=60)
                                    GGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTC                                                                                                                 >  SRR19973065.698108/1‑150 (MQ=60)
                                    GGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTC                                                                                                                 >  SRR19973065.376570/1‑150 (MQ=60)
                                    GGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTC                                                                                                                 >  SRR19973065.376571/1‑150 (MQ=60)
                                    GGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACACGTCTC                                                                                                                 >  SRR19973065.376611/1‑150 (MQ=60)
                                     GTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCT                                                                                                                <  SRR19973065.431433/150‑1 (MQ=60)
                                     GTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCT                                                                                                                >  SRR19973065.1049234/1‑150 (MQ=60)
                                     GTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCT                                                                                                                <  SRR19973065.2424639/150‑1 (MQ=60)
                                     GTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGAAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCT                                                                                                                <  SRR19973065.3179014/150‑1 (MQ=60)
                                      TATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAATCCCGTCTCTG                                                                                                               >  SRR19973065.2172678/1‑150 (MQ=60)
                                      TATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTG                                                                                                               <  SRR19973065.1450440/150‑1 (MQ=60)
                                      TATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTG                                                                                                               <  SRR19973065.3418033/150‑1 (MQ=60)
                                        TTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCT                                                                                                             >  SRR19973065.2250510/1‑150 (MQ=60)
                                        TTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCT                                                                                                             >  SRR19973065.2250448/1‑150 (MQ=60)
                                         TTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTG                                                                                                            <  SRR19973065.646741/150‑1 (MQ=60)
                                         TTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTG                                                                                                            <  SRR19973065.394778/150‑1 (MQ=60)
                                         TTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTG                                                                                                            <  SRR19973065.3231396/150‑1 (MQ=60)
                                          TATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGA                                                                                                           >  SRR19973065.892390/1‑150 (MQ=60)
                                            TTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGC                                                                                                         <  SRR19973065.2367365/150‑1 (MQ=60)
                                                GATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCA                                                                                                     <  SRR19973065.398308/150‑1 (MQ=60)
                                                 ATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCAC                                                                                                    <  SRR19973065.1095300/150‑1 (MQ=60)
                                                 ATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCGGAGCATCAC                                                                                                    >  SRR19973065.1000089/1‑150 (MQ=60)
                                                  TGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACG                                                                                                   >  SRR19973065.3522596/1‑150 (MQ=60)
                                                  TGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACG                                                                                                   <  SRR19973065.1757072/150‑1 (MQ=60)
                                                  TGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACG                                                                                                   <  SRR19973065.1757091/150‑1 (MQ=60)
                                                       TTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAAT                                                                                              <  SRR19973065.2480424/150‑1 (MQ=60)
                                                       TTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTATTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAAT                                                                                              >  SRR19973065.694315/1‑150 (MQ=60)
                                                         CTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAA                                                                                            <  SRR19973065.1953526/150‑1 (MQ=60)
                                                         CTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAA                                                                                            <  SRR19973065.234385/150‑1 (MQ=60)
                                                           TCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGG                                                                                          <  SRR19973065.2374433/150‑1 (MQ=60)
                                                           TCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGG                                                                                          <  SRR19973065.2437180/150‑1 (MQ=60)
                                                           TCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGG                                                                                          <  SRR19973065.2437175/150‑1 (MQ=60)
                                                           TCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGG                                                                                          <  SRR19973065.2436201/150‑1 (MQ=60)
                                                             GGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGC                                                                                        <  SRR19973065.696303/150‑1 (MQ=60)
                                                               CGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGG                                                                                      >  SRR19973065.3484281/1‑150 (MQ=60)
                                                                     TACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTT                                                                                >  SRR19973065.1092148/1‑150 (MQ=60)
                                                                       CTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCG                                                                              <  SRR19973065.648686/150‑1 (MQ=60)
                                                                         GGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGT                                                                            >  SRR19973065.1625633/1‑150 (MQ=60)
                                                                             CAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGT                                                                        <  SRR19973065.851779/150‑1 (MQ=60)
                                                                             CAACTTAGCGCCAGTACTCCATCCCCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGT                                                                        <  SRR19973065.2073999/150‑1 (MQ=60)
                                                                              AATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTT                                                                       >  SRR19973065.1950302/1‑150 (MQ=60)
                                                                              AATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTT                                                                       >  SRR19973065.1950362/1‑150 (MQ=60)
                                                                              AATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTT                                                                       >  SRR19973065.1951828/1‑150 (MQ=60)
                                                                              AATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTT                                                                       <  SRR19973065.351572/150‑1 (MQ=60)
                                                                               ATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTT                                                                      >  SRR19973065.3183889/1‑150 (MQ=60)
                                                                               ATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTT                                                                      >  SRR19973065.3184001/1‑150 (MQ=60)
                                                                               ATTTATCGCCAGTTCTCTATCACCATCGTTTAGGCAATGGCGCTTTCTGTTCTGGTGGCATTGTTTCTTCACCCTGCGTTATGTGCATCGCTGCTTTAACCCTTCTTTGCTGAGGATCTGTTTAATAAGGGCGGTTTCTTCGGTTGGTTT                                                                      <  SRR19973065.512046/150‑1 (MQ=60)
                                                                                 TTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAA                                                                    >  SRR19973065.1573407/1‑150 (MQ=60)
                                                                                  TATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAAT                                                                   >  SRR19973065.1428058/1‑150 (MQ=60)
                                                                                  TATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAAT                                                                   >  SRR19973065.1430029/1‑150 (MQ=60)
                                                                                   ATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATA                                                                  >  SRR19973065.168678/1‑150 (MQ=60)
                                                                                    TCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATAC                                                                 <  SRR19973065.3321286/150‑1 (MQ=60)
                                                                                     CGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACC                                                                <  SRR19973065.1020390/150‑1 (MQ=60)
                                                                                       CCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCAC                                                              <  SRR19973065.789986/150‑1 (MQ=60)
                                                                                         AGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCT                                                            >  SRR19973065.1858559/1‑150 (MQ=60)
                                                                                         AGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCT                                                            >  SRR19973065.2364644/1‑150 (MQ=60)
                                                                                          GTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTT                                                           <  SRR19973065.3017357/150‑1 (MQ=60)
                                                                                          GTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTT                                                           <  SRR19973065.2459919/150‑1 (MQ=60)
                                                                                          GTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTT                                                           <  SRR19973065.2701977/150‑1 (MQ=60)
                                                                                            TCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCGTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCG                                                         <  SRR19973065.1404850/150‑1 (MQ=60)
                                                                                             CTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGA                                                        >  SRR19973065.504709/1‑150 (MQ=60)
                                                                                             CTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGA                                                        <  SRR19973065.2864128/150‑1 (MQ=60)
                                                                                             CTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGA                                                        <  SRR19973065.2863908/150‑1 (MQ=60)
                                                                                             CTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTATTCGGTTGGTTTAATACCACCTTCGA                                                        >  SRR19973065.2428313/1‑150 (MQ=60)
                                                                                               CTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATC                                                      <  SRR19973065.1581464/150‑1 (MQ=60)
                                                                                                 ATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCAT                                                    >  SRR19973065.1998275/1‑150 (MQ=60)
                                                                                                 ATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCAT                                                    >  SRR19973065.1999265/1‑150 (MQ=60)
                                                                                                 ATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGGTTAATACCACCTTCGATCAT                                                    >  SRR19973065.2091281/1‑150 (MQ=60)
                                                                                                  TCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATA                                                   <  SRR19973065.1296289/150‑1 (MQ=60)
                                                                                                   CACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAG                                                  <  SRR19973065.3101585/150‑1 (MQ=60)
                                                                                                   CACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAG                                                  >  SRR19973065.2806160/1‑150 (MQ=60)
                                                                                                   CACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAG                                                  >  SRR19973065.243353/1‑150 (MQ=60)
                                                                                                      CATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGT                                               <  SRR19973065.1235884/150‑1 (MQ=60)
                                                                                                      CATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGT                                               >  SRR19973065.2792846/1‑150 (MQ=60)
                                                                                                        TCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTA                                             <  SRR19973065.3351671/150‑1 (MQ=60)
                                                                                                          GTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAAC                                           >  SRR19973065.1467282/1‑150 (MQ=60)
                                                                                                             TCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCAC                                        <  SRR19973065.2664011/150‑1 (MQ=60)
                                                                                                             TCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCAC                                        <  SRR19973065.1834689/150‑1 (MQ=60)
                                                                                                             TCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCGTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCAC                                        <  SRR19973065.1834784/150‑1 (MQ=60)
                                                                                                               GGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATTTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCAAAACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATAACACCTTAGATCATAGCGTTAACCACTA                                      >  SRR19973065.1405700/1‑150 (MQ=60)
                                                                                                               GGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATATCGTTAAACACTA                                      >  SRR19973065.1405703/1‑150 (MQ=60)
                                                                                                               GGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATAACACCTTCGATAATAGCGTTAACCACTA                                      >  SRR19973065.1382806/1‑150 (MQ=60)
                                                                                                                     GGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAA                                >  SRR19973065.1373693/1‑150 (MQ=60)
                                                                                                                        GCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAG                             >  SRR19973065.2739154/1‑150 (MQ=60)
                                                                                                                          TTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCG                           <  SRR19973065.662837/150‑1 (MQ=60)
                                                                                                                               GTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGC                      >  SRR19973065.1465780/1‑150 (MQ=60)
                                                                                                                                   TGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAA                  >  SRR19973065.2215986/1‑150 (MQ=60)
                                                                                                                                   TGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAA                  >  SRR19973065.1279981/1‑150 (MQ=60)
                                                                                                                                    GGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAAT                 >  SRR19973065.1828271/1‑150 (MQ=60)
                                                                                                                                    GGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAAT                 >  SRR19973065.37303/1‑150 (MQ=60)
                                                                                                                                    GGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAAT                 <  SRR19973065.1050122/150‑1 (MQ=60)
                                                                                                                                         CATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCG            <  SRR19973065.1226109/150‑1 (MQ=60)
                                                                                                                                         CATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCG            <  SRR19973065.1226079/150‑1 (MQ=60)
                                                                                                                                              ATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCC       >  SRR19973065.2745555/1‑150 (MQ=60)
                                                                                                                                                 CTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCACA    >  SRR19973065.961081/1‑150 (MQ=60)
                                                                                                                                                  TTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCACAG   >  SRR19973065.1508461/1‑150 (MQ=60)
                                                                                                                                                   TCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCACAGG  >  SRR19973065.3009682/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                    
GGGCGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCACAGG  >  CP009273/3409708‑3410004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑