Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,409,856 A→C 100% T489P (ACC→CCC)  acrF → multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,409,8560AC100.0% 860.1 / NA 213T489P (ACC→CCC) acrFmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (107/106);  total (107/106)
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 217 total aligned reads displayed.

GCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCACAG  >  CP009273/3409712‑3410003
                                                                                                                                                |                                                                                                                                                   
gcACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTcccc                                                                                                                                                  <  2:464332/148‑1 (MQ=255)
gcACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTcccc                                                                                                                                                  <  2:3427152/148‑1 (MQ=255)
   cTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTg                                                                                                                                               >  2:1693934/1‑148 (MQ=255)
    tggtggGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGc                                                                                                                                              >  2:2202950/1‑148 (MQ=255)
     ggtggGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCg                                                                                                                                             >  1:2112810/1‑148 (MQ=255)
        gggTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGTCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTa                                                                                                                                          <  1:1396974/148‑1 (MQ=255)
          gTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTAtg                                                                                                                                        <  2:693550/148‑1 (MQ=255)
          gTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTAtg                                                                                                                                        >  1:1941043/1‑148 (MQ=255)
           tATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTATTCCCCCTGCGTTAtgt                                                                                                                                       >  1:2024534/1‑148 (MQ=255)
            aTCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTAtgtg                                                                                                                                      >  1:2969288/1‑148 (MQ=255)
              cgcgATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCa                                                                                                                                    >  2:811436/1‑148 (MQ=255)
                     gTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACgctgc                                                                                                                             <  1:3004372/148‑1 (MQ=255)
                     gTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACgctgc                                                                                                                             >  1:2429436/1‑148 (MQ=255)
                      tGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACgctgct                                                                                                                            >  1:242373/1‑148 (MQ=255)
                      tGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACgctgct                                                                                                                            >  1:1692616/1‑148 (MQ=255)
                          gTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCACTTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTaaa                                                                                                                        >  1:2796256/1‑148 (MQ=255)
                          gTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTaaa                                                                                                                        <  2:2395395/148‑1 (MQ=255)
                               cGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGt                                                                                                                   >  2:1757360/1‑148 (MQ=255)
                               cGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGt                                                                                                                   >  1:928320/1‑148 (MQ=255)
                                 gTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGtct                                                                                                                 <  1:1002904/148‑1 (MQ=255)
                                 gTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGtct                                                                                                                 >  1:505392/1‑148 (MQ=255)
                                 gTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGtct                                                                                                                 <  1:728896/148‑1 (MQ=255)
                                  tatttattCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGtctc                                                                                                                >  2:2984217/1‑148 (MQ=255)
                                    tttattCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTctg                                                                                                              >  2:2862531/1‑148 (MQ=255)
                                     ttattCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTctgc                                                                                                             <  1:2500768/148‑1 (MQ=255)
                                       attCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTctgctg                                                                                                           <  1:2591534/148‑1 (MQ=255)
                                        ttCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGa                                                                                                          >  2:1151993/1‑148 (MQ=255)
                                            gATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCAt                                                                                                      <  1:2888031/148‑1 (MQ=255)
                                              tGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCa                                                                                                    <  2:52043/148‑1 (MQ=255)
                                                gCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACg                                                                                                  >  1:2615546/1‑148 (MQ=255)
                                                    tcttcGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGaaaa                                                                                              >  1:596916/1‑148 (MQ=255)
                                                       tcGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATaa                                                                                           <  1:2299805/148‑1 (MQ=255)
                                                         ggcggTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAgg                                                                                         <  2:3162295/148‑1 (MQ=255)
                                                          gcggTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAggg                                                                                        <  1:2351660/148‑1 (MQ=255)
                                                             gTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCgg                                                                                     <  2:279879/148‑1 (MQ=255)
                                                              ttCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGt                                                                                    <  2:3652370/148‑1 (MQ=255)
                                                                cTACTGGGTCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGttt                                                                                  <  1:372336/148‑1 (MQ=255)
                                                                cTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGttt                                                                                  <  1:1029650/148‑1 (MQ=255)
                                                                cTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGttt                                                                                  <  1:1437856/148‑1 (MQ=255)
                                                                  aCTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTttct                                                                                >  1:3040468/1‑148 (MQ=255)
                                                                   cTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTttctt                                                                               <  2:2264434/148‑1 (MQ=255)
                                                                    tGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTttcttc                                                                              >  2:1118641/1‑148 (MQ=255)
                                                                       ggCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCggt                                                                           <  2:1539149/148‑1 (MQ=255)
                                                                       ggCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCggt                                                                           >  2:2442464/1‑148 (MQ=255)
                                                                          aaTTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCggttgg                                                                        <  1:1973861/148‑1 (MQ=255)
                                                                           aTTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCggttggt                                                                       <  2:564782/148‑1 (MQ=255)
                                                                             ttATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGttt                                                                     <  1:1374090/148‑1 (MQ=255)
                                                                               aTCGCCAGTTCTCTATCAGCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTaa                                                                   <  1:1053362/148‑1 (MQ=255)
                                                                               aTCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTaa                                                                   <  2:2847659/148‑1 (MQ=255)
                                                                                tCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAAt                                                                  >  2:2554735/1‑148 (MQ=255)
                                                                                 cGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGGTGGTTTAATa                                                                 >  1:2705774/1‑148 (MQ=255)
                                                                                    cAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGATTGGTTTAATacca                                                              <  2:3080555/148‑1 (MQ=255)
                                                                                     aGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCACCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATaccac                                                             <  2:1433246/148‑1 (MQ=255)
                                                                                      gTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCTGTTTCTTCGGTTGGTTTAATaccacc                                                            >  1:1141699/1‑148 (MQ=255)
                                                                                      gTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAAACCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATaccacc                                                            >  1:672386/1‑148 (MQ=255)
                                                                                       ttCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCAACt                                                           >  2:181977/1‑148 (MQ=255)
                                                                                       ttCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGAGGTTTCTTCGGTTGGTTTAATACCACCt                                                           >  2:1882252/1‑148 (MQ=255)
                                                                                       ttCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGAGGTTTCTTCGGTTGGTTTAATACCACCt                                                           >  2:3539543/1‑148 (MQ=255)
                                                                                        tctcTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCtt                                                          >  2:2200118/1‑148 (MQ=255)
                                                                                          tctATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCg                                                        <  1:2202797/148‑1 (MQ=255)
                                                                                           ctATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGa                                                       >  2:1205737/1‑148 (MQ=255)
                                                                                             aTCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATc                                                     >  1:1258159/1‑148 (MQ=255)
                                                                                              tCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCa                                                    >  2:3295737/1‑148 (MQ=255)
                                                                                              tCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCa                                                    <  2:3364026/148‑1 (MQ=255)
                                                                                              tCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCa                                                    >  2:2300932/1‑148 (MQ=255)
                                                                                              tCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCa                                                    >  2:1186578/1‑148 (MQ=255)
                                                                                               cACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCAt                                                   <  1:415995/148‑1 (MQ=255)
                                                                                                aCCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATa                                                  >  1:111285/1‑148 (MQ=255)
                                                                                                aCCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAAACCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATAACACCTTCGAACATa                                                  >  1:3166372/1‑148 (MQ=255)
                                                                                                 ccATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAg                                                 <  2:242373/148‑1 (MQ=255)
                                                                                                   aTCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCg                                               >  1:180392/1‑148 (MQ=255)
                                                                                                   aTCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCg                                               >  2:1369189/1‑148 (MQ=255)
                                                                                                    tCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGt                                              >  2:3383407/1‑148 (MQ=255)
                                                                                                      gTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTa                                            <  2:60754/148‑1 (MQ=255)
                                                                                                      gTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTa                                            <  2:1992545/148‑1 (MQ=255)
                                                                                                        ttCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGAGTCTTCACCCTGCGTTATGTGCAACGCTTCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAAc                                          <  1:1615720/148‑1 (MQ=255)
                                                                                                               aTGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTAGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTaca                                   >  1:2678103/1‑148 (MQ=255)
                                                                                                                 ggCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCAACTTCGATCATAGCGTTAACCACTACAcc                                 >  1:300125/1‑148 (MQ=255)
                                                                                                                  gcgcTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCa                                >  1:223157/1‑148 (MQ=255)
                                                                                                                    gcTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAAc                              <  2:656700/148‑1 (MQ=255)
                                                                                                                    gcTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAAc                              >  2:1868966/1‑148 (MQ=255)
                                                                                                                      tttCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAg                            <  2:2733126/148‑1 (MQ=255)
                                                                                                                       ttctgttctgGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGc                           >  2:1529131/1‑148 (MQ=255)
                                                                                                                        tctgttctgGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTCCGATCATAGCGTTAACCACTACACCAACAGCg                          <  2:1078980/148‑1 (MQ=255)
                                                                                                                          tgttctgGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTc                        <  1:326557/148‑1 (MQ=255)
                                                                                                                           gttctgGTGTCATTGATTCTTCCCCCTGCTTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCg                       <  1:1415496/148‑1 (MQ=255)
                                                                                                                           gttctgGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCg                       >  1:2726289/1‑148 (MQ=255)
                                                                                                                                ggtggCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCaaa                  >  1:17416/1‑148 (MQ=255)
                                                                                                                                ggtggCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCaaa                  <  2:3712386/148‑1 (MQ=255)
                                                                                                                                ggtggCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCaaa                  <  2:3712460/148‑1 (MQ=255)
                                                                                                                                      aTTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTc            <  2:3602335/148‑1 (MQ=255)
                                                                                                                                      aTTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTc            >  2:1375124/1‑148 (MQ=255)
                                                                                                                                       ttGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCg           <  2:1935687/148‑1 (MQ=255)
                                                                                                                                        tGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCgg          >  1:1363640/1‑148 (MQ=255)
                                                                                                                                          aTTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGAt        >  2:2139373/1‑148 (MQ=255)
                                                                                                                                          aTTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGAt        <  2:2916779/148‑1 (MQ=255)
                                                                                                                                          aTTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGAt        >  2:249102/1‑148 (MQ=255)
                                                                                                                                            tCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATcc      <  1:2752721/148‑1 (MQ=255)
                                                                                                                                            tCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATcc      <  1:1531477/148‑1 (MQ=255)
                                                                                                                                            tCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATcc      <  1:2862531/148‑1 (MQ=255)
                                                                                                                                                |                                                                                                                                                   
GCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCACAG  >  CP009273/3409712‑3410003

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGGGCGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCACAGG  >  CP009273/3409707‑3410004
                                                                                                                                                     |                                                                                                                                                    
AGGGCGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTC                                                                                                                                                      >  SRR19973069.661114/1‑150 (MQ=60)
AGGGCGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTC                                                                                                                                                      <  SRR19973069.2119935/150‑1 (MQ=60)
AGGGCGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTC                                                                                                                                                      >  SRR19973069.3004107/1‑150 (MQ=60)
AGGGCGCACTGGTGGGTATCGCGATGGAGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTC                                                                                                                                                      >  SRR19973069.1086506/1‑150 (MQ=60)
        CTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCG                                                                                                                                              >  SRR19973069.2127378/1‑150 (MQ=60)
        CTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCG                                                                                                                                              >  SRR19973069.2124925/1‑150 (MQ=60)
          GGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGCCATTGATTCTTCCCCCTGCGTT                                                                                                                                            <  SRR19973069.1614404/150‑1 (MQ=60)
           GTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTA                                                                                                                                           >  SRR19973069.2006432/1‑150 (MQ=60)
           GTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTA                                                                                                                                           >  SRR19973069.61479/1‑150 (MQ=60)
             GGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGTCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATG                                                                                                                                         <  SRR19973069.1406722/150‑1 (MQ=60)
             GGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATG                                                                                                                                         <  SRR19973069.1406485/150‑1 (MQ=60)
             GGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATG                                                                                                                                         >  SRR19973069.1954410/1‑150 (MQ=60)
              GGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTATTCCCCCTGCGTTATGT                                                                                                                                        >  SRR19973069.2038594/1‑150 (MQ=60)
               GTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTG                                                                                                                                       >  SRR19973069.2988997/1‑150 (MQ=60)
                        TGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGC                                                                                                                              >  SRR19973069.2445824/1‑150 (MQ=60)
                         GGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCT                                                                                                                             >  SRR19973069.244183/1‑150 (MQ=60)
                         GGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCT                                                                                                                             >  SRR19973069.1704438/1‑150 (MQ=60)
                          GTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTT                                                                                                                            <  SRR19973069.3024307/150‑1 (MQ=60)
                             CTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCACTTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAA                                                                                                                         >  SRR19973069.2814836/1‑150 (MQ=60)
                                 CAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCG                                                                                                                     >  SRR19973069.164681/1‑150 (MQ=60)
                                  AGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGT                                                                                                                    >  SRR19973069.934627/1‑150 (MQ=60)
                                    CGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCT                                                                                                                  >  SRR19973069.2442591/1‑150 (MQ=60)
                                    CGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCT                                                                                                                  <  SRR19973069.2534010/150‑1 (MQ=60)
                                    CGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCT                                                                                                                  >  SRR19973069.2751418/1‑150 (MQ=60)
                                    CGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCT                                                                                                                  >  SRR19973069.509006/1‑150 (MQ=60)
                                     GGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTC                                                                                                                 >  SRR19973069.1414704/1‑150 (MQ=60)
                                     GGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTC                                                                                                                 >  SRR19973069.555479/1‑150 (MQ=60)
                                      GTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCT                                                                                                                <  SRR19973069.733802/150‑1 (MQ=60)
                                      GTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCT                                                                                                                <  SRR19973069.734046/150‑1 (MQ=60)
                                      GTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCT                                                                                                                <  SRR19973069.1011933/150‑1 (MQ=60)
                                      GTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCT                                                                                                                <  SRR19973069.1009789/150‑1 (MQ=60)
                                         TTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCT                                                                                                             <  SRR19973069.2672699/150‑1 (MQ=60)
                                          TTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTG                                                                                                            <  SRR19973069.2517469/150‑1 (MQ=60)
                                            ATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAG                                                                                                          <  SRR19973069.2608813/150‑1 (MQ=60)
                                               CCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCAT                                                                                                       >  SRR19973069.758576/1‑150 (MQ=60)
                                                 GATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCA                                                                                                     <  SRR19973069.2907051/150‑1 (MQ=60)
                                                 GATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCA                                                                                                     <  SRR19973069.2907027/150‑1 (MQ=60)
                                                  ATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCAC                                                                                                    >  SRR19973069.2866423/1‑150 (MQ=60)
                                                   TGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACG                                                                                                   >  SRR19973069.2633027/1‑150 (MQ=60)
                                                       ATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAA                                                                                               >  SRR19973069.600952/1‑150 (MQ=60)
                                                         TCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATA                                                                                             <  SRR19973069.833778/150‑1 (MQ=60)
                                                            TCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGG                                                                                          <  SRR19973069.2315435/150‑1 (MQ=60)
                                                               GCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCG                                                                                       <  SRR19973069.2368238/150‑1 (MQ=60)
                                                               GCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCG                                                                                       <  SRR19973069.2367650/150‑1 (MQ=60)
                                                                    TCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTC                                                                                  <  SRR19973069.1726103/150‑1 (MQ=60)
                                                                     CTACTGGGTCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCT                                                                                 <  SRR19973069.375052/150‑1 (MQ=60)
                                                                     CTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCT                                                                                 >  SRR19973069.3060613/1‑150 (MQ=60)
                                                                     CTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCT                                                                                 <  SRR19973069.1447405/150‑1 (MQ=60)
                                                                     CTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCT                                                                                 <  SRR19973069.1447890/150‑1 (MQ=60)
                                                                     CTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCT                                                                                 >  SRR19973069.3036039/1‑150 (MQ=60)
                                                                     CTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCT                                                                                 >  SRR19973069.40746/1‑150 (MQ=60)
                                                                     CTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCT                                                                                 <  SRR19973069.1036783/150‑1 (MQ=60)
                                                                     CTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCT                                                                                 <  SRR19973069.2026771/150‑1 (MQ=60)
                                                                      TACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTT                                                                                <  SRR19973069.2713757/150‑1 (MQ=60)
                                                                      TACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTT                                                                                <  SRR19973069.2713748/150‑1 (MQ=60)
                                                                               AATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTT                                                                       <  SRR19973069.1987577/150‑1 (MQ=60)
                                                                                ATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTT                                                                      >  SRR19973069.3071959/1‑150 (MQ=60)
                                                                                ATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTT                                                                      <  SRR19973069.1351744/150‑1 (MQ=60)
                                                                                ATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTT                                                                      <  SRR19973069.2995290/150‑1 (MQ=60)
                                                                                  TTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAA                                                                    <  SRR19973069.1383667/150‑1 (MQ=60)
                                                                                   TATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAAT                                                                   <  SRR19973069.3022729/150‑1 (MQ=60)
                                                                                    ATCGCCAGTTCTCTATCAGCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATA                                                                  <  SRR19973069.1060672/150‑1 (MQ=60)
                                                                                    ATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATA                                                                  <  SRR19973069.1060483/150‑1 (MQ=60)
                                                                                    ATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGGTGGTTTAATA                                                                  >  SRR19973069.2723915/1‑150 (MQ=60)
                                                                                     TCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATAC                                                                 <  SRR19973069.2425360/150‑1 (MQ=60)
                                                                                         CAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATTTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGGTTAATACCACC                                                             >  SRR19973069.2610356/1‑150 (MQ=60)
                                                                                         CAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCTGTTTCTTCGGTTGGTTTAATACCACC                                                             >  SRR19973069.1149665/1‑150 (MQ=60)
                                                                                         CAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAAACCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGGTTCTTCGGTTGGTTTTATACCACC                                                             >  SRR19973069.677011/1‑150 (MQ=60)
                                                                                          AGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTATTCCCCCTGCGTTATTTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTTGTTTAATACCACCT                                                            >  SRR19973069.18881/1‑150 (MQ=60)
                                                                                           GTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTT                                                           >  SRR19973069.1982350/1‑150 (MQ=60)
                                                                                               TCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGAT                                                       <  SRR19973069.2217899/150‑1 (MQ=60)
                                                                                               TCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGAT                                                       <  SRR19973069.2217746/150‑1 (MQ=60)
                                                                                                CTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATC                                                      >  SRR19973069.1266654/1‑150 (MQ=60)
                                                                                                CTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATC                                                      >  SRR19973069.1266652/1‑150 (MQ=60)
                                                                                                CTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATC                                                      <  SRR19973069.1493277/150‑1 (MQ=60)
                                                                                                CTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATC                                                      <  SRR19973069.2968070/150‑1 (MQ=60)
                                                                                                CTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATC                                                      <  SRR19973069.2968095/150‑1 (MQ=60)
                                                                                                   TCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATA                                                   >  SRR19973069.112377/1‑150 (MQ=60)
                                                                                                   TCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAAACCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATAACACCTTCGAACATA                                                   >  SRR19973069.3187010/1‑150 (MQ=60)
                                                                                                    CACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAG                                                  <  SRR19973069.419045/150‑1 (MQ=60)
                                                                                                     ACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGC                                                 >  SRR19973069.2187085/1‑150 (MQ=60)
                                                                                                      CCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCG                                                >  SRR19973069.181897/1‑150 (MQ=60)
                                                                                                      CCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAAACCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCG                                                >  SRR19973069.2355989/1‑150 (MQ=60)
                                                                                                        ATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTT                                              <  SRR19973069.937919/150‑1 (MQ=60)
                                                                                                          CGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATAACACCTTCGATCATAGCGTTAA                                            >  SRR19973069.2470295/1‑150 (MQ=60)
                                                                                                            TTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACC                                          <  SRR19973069.439222/150‑1 (MQ=60)
                                                                                                             TTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCA                                         <  SRR19973069.1100592/150‑1 (MQ=60)
                                                                                                             TTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGAGTCTTCACCCTGCGTTATGTGCAACGCTTCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCA                                         <  SRR19973069.1626626/150‑1 (MQ=60)
                                                                                                                  CNATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTAGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACA                                    >  SRR19973069.2696057/1‑150 (MQ=60)
                                                                                                                    ATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACC                                  <  SRR19973069.3271101/150‑1 (MQ=60)
                                                                                                                    ATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCAACTTCGATCATAGCGTTAACCACTACACC                                  >  SRR19973069.302162/1‑150 (MQ=60)
                                                                                                                     TGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCA                                 >  SRR19973069.224879/1‑150 (MQ=60)
                                                                                                                     TGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCA                                 >  SRR19973069.224883/1‑150 (MQ=60)
                                                                                                                              CTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCG                        >  SRR19973069.2744549/1‑150 (MQ=60)
                                                                                                                              CTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCG                        >  SRR19973069.2744494/1‑150 (MQ=60)
                                                                                                                               TGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGG                       <  SRR19973069.328753/150‑1 (MQ=60)
                                                                                                                                GTTCTGGTGTCATTGATTCTTCCCCCTGCTTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGC                      <  SRR19973069.1425364/150‑1 (MQ=60)
                                                                                                                                GTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGAATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGC                      <  SRR19973069.1543509/150‑1 (MQ=60)
                                                                                                                                   CTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAA                   >  SRR19973069.17660/1‑150 (MQ=60)
                                                                                                                                           ATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGG           >  SRR19973069.1373627/1‑150 (MQ=60)
                                                                                                                                           ATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGG           >  SRR19973069.1373121/1‑150 (MQ=60)
                                                                                                                                           ATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGG           >  SRR19973069.2791861/1‑150 (MQ=60)
                                                                                                                                             TGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGAT         >  SRR19973069.1142878/1‑150 (MQ=60)
                                                                                                                                               ATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCC       <  SRR19973069.1214023/150‑1 (MQ=60)
                                                                                                                                                TTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCA      <  SRR19973069.3497644/150‑1 (MQ=60)
                                                                                                                                                 TCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCAC     <  SRR19973069.2771096/150‑1 (MQ=60)
                                                                                                                                                 TCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCAC     <  SRR19973069.1542001/150‑1 (MQ=60)
                                                                                                                                                 TCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCAC     <  SRR19973069.2881348/150‑1 (MQ=60)
                                                                                                                                                  CTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCACA    <  SRR19973069.3673482/150‑1 (MQ=60)
                                                                                                                                                  CTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCACA    <  SRR19973069.1769740/150‑1 (MQ=60)
                                                                                                                                                    TCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCACAGG  <  SRR19973069.740338/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
AGGGCGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCACAGG  >  CP009273/3409707‑3410004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑