Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F368 I0 R1 1472 214.4 7116750 95.9% 6824963 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,409,856 A→C 100% T489P (ACC→CCC)  acrF → multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,409,8560AC97.3% 882.7 / 3.3 230T489P (ACC→CCC) acrFmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base A (3/4);  new base C (134/89);  total (137/93)
Fisher's exact test for biased strand distribution p-value = 4.45e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 232 total aligned reads displayed.

CGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCA  >  CP009273/3409711‑3410000
                                                                                                                                                 |                                                                                                                                                
cgcACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTccc                                                                                                                                                <  1:2486827/148‑1 (MQ=255)
cgcACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTccc                                                                                                                                                <  2:1019282/148‑1 (MQ=255)
          ggTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGAGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTAt                                                                                                                                      <  1:1355710/148‑1 (MQ=255)
          ggTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGAGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTAt                                                                                                                                      <  1:3125186/148‑1 (MQ=255)
             aTCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTAtgtg                                                                                                                                   >  1:1192587/1‑148 (MQ=255)
             aTCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTAtgtg                                                                                                                                   >  2:1123039/1‑148 (MQ=255)
              tCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGc                                                                                                                                  >  1:2195598/1‑148 (MQ=255)
                gcgATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCaa                                                                                                                                <  1:346452/148‑1 (MQ=255)
                    tGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACgct                                                                                                                            >  2:218668/1‑148 (MQ=255)
                     ggTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACgctg                                                                                                                           >  2:959538/1‑148 (MQ=255)
                     ggTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACgctg                                                                                                                           <  1:2887372/148‑1 (MQ=255)
                     ggTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTCCGTTATGTGCAACgctg                                                                                                                           <  2:1822799/148‑1 (MQ=255)
                      gTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACgctgc                                                                                                                          >  2:2534383/1‑148 (MQ=255)
                         cTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTa                                                                                                                       <  2:1615525/148‑1 (MQ=255)
                           gTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTaaa                                                                                                                     <  2:1893793/148‑1 (MQ=255)
                                cGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGt                                                                                                                >  1:501638/1‑148 (MQ=255)
                                    atttattCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTctct                                                                                                            >  2:381628/1‑148 (MQ=255)
                                    atttattCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTctct                                                                                                            >  2:1168215/1‑148 (MQ=255)
                                    atttattCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTctct                                                                                                            >  1:1053236/1‑148 (MQ=255)
                                    atttattCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTctct                                                                                                            >  1:2065464/1‑148 (MQ=255)
                                     tttattCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTctg                                                                                                           >  2:181472/1‑148 (MQ=255)
                                         ttCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGa                                                                                                       <  1:144117/148‑1 (MQ=255)
                                          tCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAg                                                                                                      <  2:1080429/148‑1 (MQ=255)
                                          tCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAg                                                                                                      <  2:1079994/148‑1 (MQ=255)
                                             gATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGGGCAt                                                                                                   >  1:1810847/1‑148 (MQ=255)
                                              aTGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATc                                                                                                  <  1:1541167/148‑1 (MQ=255)
                                                ggCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCAc                                                                                                >  2:1977779/1‑148 (MQ=255)
                                                  cATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGa                                                                                              >  1:1887898/1‑148 (MQ=255)
                                                  cATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGa                                                                                              >  2:2151234/1‑148 (MQ=255)
                                                    ttcttcGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGaaa                                                                                            <  1:1948128/148‑1 (MQ=255)
                                                     tcttcGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGTCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGaaaa                                                                                           >  1:3251196/1‑148 (MQ=255)
                                                       ttcGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATa                                                                                         <  2:2670314/148‑1 (MQ=255)
                                                       ttcGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATa                                                                                         <  2:2670671/148‑1 (MQ=255)
                                                             ggTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCg                                                                                   >  2:1106036/1‑148 (MQ=255)
                                                                tCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGtt                                                                                <  2:754542/148‑1 (MQ=255)
                                                                tCTACTGGGGCAATTGATCGCCAGTTCTCTATCACCATCGTTTCTGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATTATGGCGGtt                                                                                <  2:2847283/148‑1 (MQ=255)
                                                                   aCTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTttct                                                                             <  1:1683516/148‑1 (MQ=255)
                                                                      ggggCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCg                                                                          <  1:3215450/148‑1 (MQ=255)
                                                                      ggggCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCg                                                                          <  1:2271236/148‑1 (MQ=255)
                                                                       gggCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCGTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCgg                                                                         >  2:1527281/1‑148 (MQ=255)
                                                                        ggCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCggt                                                                        >  1:1129727/1‑148 (MQ=255)
                                                                          cAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCGCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCggttg                                                                      >  1:2098070/1‑148 (MQ=255)
                                                                            aTTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCggttggt                                                                    >  1:3313995/1‑148 (MQ=255)
                                                                               tATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTa                                                                 <  1:2695983/148‑1 (MQ=255)
                                                                                 tCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAAt                                                               <  1:1854157/148‑1 (MQ=255)
                                                                                 tCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAAt                                                               >  2:240501/1‑148 (MQ=255)
                                                                                 tCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAAt                                                               >  2:662116/1‑148 (MQ=255)
                                                                                    ccAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATacc                                                            >  1:2370583/1‑148 (MQ=255)
                                                                                     cAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATacca                                                           <  1:439033/148‑1 (MQ=255)
                                                                                            ctATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGa                                                    >  1:1985071/1‑148 (MQ=255)
                                                                                            ctATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGa                                                    <  1:1688191/148‑1 (MQ=255)
                                                                                             tATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGAt                                                   >  1:1913731/1‑148 (MQ=255)
                                                                                             tATCACCATCGTTTCGGCAATGGCGCTTTCTGGTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGAt                                                   <  1:2138260/148‑1 (MQ=255)
                                                                                              aTCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATc                                                  <  1:1758510/148‑1 (MQ=255)
                                                                                              aTCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGCTTCTTCGGTTGGTTTAATACCACCTTCGATc                                                  <  1:3012901/148‑1 (MQ=255)
                                                                                               tCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCa                                                 <  2:458178/148‑1 (MQ=255)
                                                                                               tCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCa                                                 <  1:1255412/148‑1 (MQ=255)
                                                                                               tCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCa                                                 <  1:1255453/148‑1 (MQ=255)
                                                                                               tCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCa                                                 <  1:1390239/148‑1 (MQ=255)
                                                                                                cACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCAt                                                <  1:258279/148‑1 (MQ=255)
                                                                                                cACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCAt                                                >  2:1148765/1‑148 (MQ=255)
                                                                                                  ccATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAg                                              <  1:2553048/148‑1 (MQ=255)
                                                                                                   cATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGc                                             >  2:2655070/1‑148 (MQ=255)
                                                                                                   cATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGc                                             >  1:2658039/1‑148 (MQ=255)
                                                                                                     tCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGt                                           >  2:1497971/1‑148 (MQ=255)
                                                                                                      cGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGtt                                          >  2:2461407/1‑148 (MQ=255)
                                                                                                       gTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTa                                         <  2:2964140/148‑1 (MQ=255)
                                                                                                        tttCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTaa                                        >  1:548598/1‑148 (MQ=255)
                                                                                                         ttCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGGTTAATACCACCTTCGATCATAGCGTTAAc                                       >  2:1788992/1‑148 (MQ=255)
                                                                                                         ttCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAAAGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAAc                                       >  1:78408/1‑148 (MQ=255)
                                                                                                          tCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAAcc                                      >  2:2153166/1‑148 (MQ=255)
                                                                                                            ggCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCAc                                    >  1:1055056/1‑148 (MQ=255)
                                                                                                             gCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACt                                   <  1:167788/148‑1 (MQ=255)
                                                                                                               aaTGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAGAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTac                                 <  2:2029247/148‑1 (MQ=255)
                                                                                                               aaTGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTac                                 <  1:3176677/148‑1 (MQ=255)
                                                                                                                 tGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTacac                               >  1:2606169/1‑148 (MQ=255)
                                                                                                                  ggCGCTTTCTGTTCTGGTGGCATTGATTATTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACAcc                              >  1:634867/1‑148 (MQ=255)
                                                                                                                   gcgcTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCa                             >  2:3556759/1‑148 (MQ=255)
                                                                                                                   gcgcTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCa                             >  1:386114/1‑148 (MQ=255)
                                                                                                                          ctgttctgGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGt                      <  1:2446721/148‑1 (MQ=255)
                                                                                                                            gttctgGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCg                    >  2:3208947/1‑148 (MQ=255)
                                                                                                                            gttctgGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCg                    >  2:3208904/1‑148 (MQ=255)
                                                                                                                             ttctgGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCgg                   >  2:2921413/1‑148 (MQ=255)
                                                                                                                             ttctgGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCgg                   >  1:2877772/1‑148 (MQ=255)
                                                                                                                                 ggtggCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCaaa               >  1:1761769/1‑148 (MQ=255)
                                                                                                                                  gtggCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGAGTTAACCACTACACCAACAGCGTCGGCaaaa              >  2:2279802/1‑148 (MQ=255)
                                                                                                                                   tggCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAAt             >  2:809713/1‑148 (MQ=255)
                                                                                                                                    ggCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATc            <  2:1729189/148‑1 (MQ=255)
                                                                                                                                     gCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATcc           >  1:3377334/1‑148 (MQ=255)
                                                                                                                                      cATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCt          >  2:482618/1‑148 (MQ=255)
                                                                                                                                      cATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCt          >  2:2019521/1‑148 (MQ=255)
                                                                                                                                        ttGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCg        >  2:2878720/1‑148 (MQ=255)
                                                                                                                                        ttGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCg        >  2:31050/1‑148 (MQ=255)
                                                                                                                                        ttGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCg        >  2:34548/1‑148 (MQ=255)
                                                                                                                                         tGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCgg       >  1:611042/1‑148 (MQ=255)
                                                                                                                                           aTTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGAt     >  1:676374/1‑148 (MQ=255)
                                                                                                                                           aTTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGAt     >  2:1271025/1‑148 (MQ=255)
                                                                                                                                           aTTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGAt     >  2:2329895/1‑148 (MQ=255)
                                                                                                                                             tCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATcc   <  1:1977779/148‑1 (MQ=255)
                                                                                                                                              cTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCca  >  2:259525/1‑148 (MQ=255)
                                                                                                                                                 |                                                                                                                                                
CGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCA  >  CP009273/3409711‑3410000

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
AGGGCGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCACAGG  >  CP009273/3409707‑3410004
                                                                                                                                                     |                                                                                                                                                    
AGGGCGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTC                                                                                                                                                      >  SRR19973067.2886785/1‑150 (MQ=60)
    CGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCC                                                                                                                                                  >  SRR19973067.3224962/1‑150 (MQ=60)
    CGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCC                                                                                                                                                  <  SRR19973067.2503169/150‑1 (MQ=60)
       ACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGC                                                                                                                                               >  SRR19973067.2026668/1‑150 (MQ=60)
          GGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTT                                                                                                                                            <  SRR19973067.2492015/150‑1 (MQ=60)
              GGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGAGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGT                                                                                                                                        <  SRR19973067.1364973/150‑1 (MQ=60)
              GGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGAGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGT                                                                                                                                        <  SRR19973067.3145048/150‑1 (MQ=60)
               GTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTG                                                                                                                                       >  SRR19973067.1200417/1‑150 (MQ=60)
               GTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTG                                                                                                                                       >  SRR19973067.2863857/1‑150 (MQ=60)
                TATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGC                                                                                                                                      >  SRR19973067.2210242/1‑150 (MQ=60)
                 ATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCA                                                                                                                                     >  SRR19973067.1451293/1‑150 (MQ=60)
                  TCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAA                                                                                                                                    <  SRR19973067.1568122/150‑1 (MQ=60)
                    GCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACG                                                                                                                                  <  SRR19973067.348846/150‑1 (MQ=60)
                     CGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGC                                                                                                                                 >  SRR19973067.1187113/1‑150 (MQ=60)
                      GATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCT                                                                                                                                <  SRR19973067.1336532/150‑1 (MQ=60)
                       ATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTG                                                                                                                               <  SRR19973067.613733/150‑1 (MQ=60)
                         GGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCT                                                                                                                             <  SRR19973067.2905829/150‑1 (MQ=60)
                         GGTGCTGTCAGCGGTATTTAGTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCT                                                                                                                             <  SRR19973067.2905772/150‑1 (MQ=60)
                          GTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTT                                                                                                                            <  SRR19973067.2464689/150‑1 (MQ=60)
                             CTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAA                                                                                                                         >  SRR19973067.461355/1‑150 (MQ=60)
                             CTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAA                                                                                                                         >  SRR19973067.461361/1‑150 (MQ=60)
                             CTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAA                                                                                                                         <  SRR19973067.304450/150‑1 (MQ=60)
                                TCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCC                                                                                                                      >  SRR19973067.2641326/1‑150 (MQ=60)
                                  AGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGT                                                                                                                    >  SRR19973067.505079/1‑150 (MQ=60)
                                    CGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCT                                                                                                                  <  SRR19973067.21720/150‑1 (MQ=60)
                                      GTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCT                                                                                                                >  SRR19973067.2079203/1‑150 (MQ=60)
                                      GTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCT                                                                                                                >  SRR19973067.2079878/1‑150 (MQ=60)
                                      GTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCT                                                                                                                >  SRR19973067.1060456/1‑150 (MQ=60)
                                          TTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAAACCGTCTCTGCTG                                                                                                            >  SRR19973067.772954/1‑150 (MQ=60)
                                           TATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGA                                                                                                           >  SRR19973067.1993709/1‑150 (MQ=60)
                                            ATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAG                                                                                                          <  SRR19973067.2067849/150‑1 (MQ=60)
                                             TTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGC                                                                                                         <  SRR19973067.145263/150‑1 (MQ=60)
                                             TTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGC                                                                                                         <  SRR19973067.145379/150‑1 (MQ=60)
                                               CCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGGGCAT                                                                                                       >  SRR19973067.1823093/1‑150 (MQ=60)
                                               CCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCAT                                                                                                       >  SRR19973067.1823207/1‑150 (MQ=60)
                                                  ATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCAC                                                                                                    >  SRR19973067.631238/1‑150 (MQ=60)
                                                  ATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCAC                                                                                                    <  SRR19973067.1551616/150‑1 (MQ=60)
                                                    GGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGA                                                                                                  >  SRR19973067.1368752/1‑150 (MQ=60)
                                                    GGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGA                                                                                                  >  SRR19973067.1900788/1‑150 (MQ=60)
                                                     GCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAA                                                                                                 >  SRR19973067.396034/1‑150 (MQ=60)
                                                     GCATTCTTCGGCGGTTCTACTGGGGCAATTAATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAA                                                                                                 >  SRR19973067.1384701/1‑150 (MQ=60)
                                                       ATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGTCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAA                                                                                               >  SRR19973067.3271539/1‑150 (MQ=60)
                                                        TTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAAT                                                                                              <  SRR19973067.1960982/150‑1 (MQ=60)
                                                        TTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAAT                                                                                              <  SRR19973067.1961401/150‑1 (MQ=60)
                                                         TCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATA                                                                                             >  SRR19973067.1590403/1‑150 (MQ=60)
                                                            TCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGG                                                                                          <  SRR19973067.218201/150‑1 (MQ=60)
                                                               GCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCG                                                                                       <  SRR19973067.1803809/150‑1 (MQ=60)
                                                                     CTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCT                                                                                 >  SRR19973067.1090269/1‑150 (MQ=60)
                                                                       ACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTC                                                                               <  SRR19973067.1695124/150‑1 (MQ=60)
                                                                        CTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCG                                                                              <  SRR19973067.3175184/150‑1 (MQ=60)
                                                                        CTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCG                                                                              <  SRR19973067.3467930/150‑1 (MQ=60)
                                                                          GGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGT                                                                            <  SRR19973067.2286279/150‑1 (MQ=60)
                                                                          GGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGT                                                                            <  SRR19973067.3140007/150‑1 (MQ=60)
                                                                          GGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGT                                                                            >  SRR19973067.1137349/1‑150 (MQ=60)
                                                                          GGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGT                                                                            <  SRR19973067.3235686/150‑1 (MQ=60)
                                                                            GGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCGCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTG                                                                          >  SRR19973067.2111971/1‑150 (MQ=60)
                                                                            GGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGGTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTG                                                                          >  SRR19973067.1907257/1‑150 (MQ=60)
                                                                              CAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGT                                                                        >  SRR19973067.3334944/1‑150 (MQ=60)
                                                                              CAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCAAGAAAATAAGGCCGGTTTCTTCGGTTGGT                                                                        >  SRR19973067.3265461/1‑150 (MQ=60)
                                                                              CAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTATGGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCAGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGT                                                                        >  SRR19973067.3335084/1‑150 (MQ=60)
                                                                                ATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTT                                                                      <  SRR19973067.1006515/150‑1 (MQ=60)
                                                                                   TATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAAT                                                                   <  SRR19973067.2713305/150‑1 (MQ=60)
                                                                                   TATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAAT                                                                   <  SRR19973067.2713323/150‑1 (MQ=60)
                                                                                     TCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATAC                                                                 <  SRR19973067.1866794/150‑1 (MQ=60)
                                                                                     TCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATAC                                                                 <  SRR19973067.639215/150‑1 (MQ=60)
                                                                                      CGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACC                                                                >  SRR19973067.2386005/1‑150 (MQ=60)
                                                                                      CGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACC                                                                >  SRR19973067.120831/1‑150 (MQ=60)
                                                                                      CGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACA                                                                >  SRR19973067.2385958/1‑150 (MQ=60)
                                                                                       GCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCA                                                               >  SRR19973067.1939458/1‑150 (MQ=60)
                                                                                       GCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCA                                                               >  SRR19973067.1736490/1‑150 (MQ=60)
                                                                                         CAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACC                                                             <  SRR19973067.442087/150‑1 (MQ=60)
                                                                                            TTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTC                                                          <  SRR19973067.1729926/150‑1 (MQ=60)
                                                                                              CTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGA                                                        >  SRR19973067.529460/1‑150 (MQ=60)
                                                                                              CTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGA                                                        >  SRR19973067.1998505/1‑150 (MQ=60)
                                                                                               TCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGAT                                                       >  SRR19973067.1926791/1‑150 (MQ=60)
                                                                                               TCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGAT                                                       >  SRR19973067.122864/1‑150 (MQ=60)
                                                                                                CTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATC                                                      <  SRR19973067.1699820/150‑1 (MQ=60)
                                                                                                CTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATC                                                      <  SRR19973067.1699692/150‑1 (MQ=60)
                                                                                                 TATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCA                                                     <  SRR19973067.1879068/150‑1 (MQ=60)
                                                                                                 TATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCA                                                     <  SRR19973067.2152532/150‑1 (MQ=60)
                                                                                                 TATCACCATCGTTTCGGCAATGGCGCTTTCTGGTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCA                                                     <  SRR19973067.2152550/150‑1 (MQ=60)
                                                                                                  ATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCAT                                                    <  SRR19973067.1770483/150‑1 (MQ=60)
                                                                                                  ATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCAT                                                    <  SRR19973067.2503306/150‑1 (MQ=60)
                                                                                                  ATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGCTTCTTCGGTTGGTTTAATACCACCTTCGATCAT                                                    <  SRR19973067.3032072/150‑1 (MQ=60)
                                                                                                   TCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATA                                                   <  SRR19973067.1399688/150‑1 (MQ=60)
                                                                                                   TCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATA                                                   <  SRR19973067.1263949/150‑1 (MQ=60)
                                                                                                   TCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATA                                                   <  SRR19973067.1263908/150‑1 (MQ=60)
                                                                                                   TCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATA                                                   <  SRR19973067.1263944/150‑1 (MQ=60)
                                                                                                    CACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAG                                                  <  SRR19973067.260043/150‑1 (MQ=60)
                                                                                                     ACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGC                                                 >  SRR19973067.2675146/1‑150 (MQ=60)
                                                                                                     ACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAAACCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATATC                                                 >  SRR19973067.3400195/1‑150 (MQ=60)
                                                                                                      CCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCG                                                <  SRR19973067.2569743/150‑1 (MQ=60)
                                                                                                      CCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCG                                                <  SRR19973067.2570340/150‑1 (MQ=60)
                                                                                                       CATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGT                                               >  SRR19973067.2620732/1‑150 (MQ=60)
                                                                                                       CATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGT                                               >  SRR19973067.2620729/1‑150 (MQ=60)
                                                                                                          CGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAA                                            >  SRR19973067.552216/1‑150 (MQ=60)
                                                                                                           GTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAAAGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAAC                                           >  SRR19973067.79285/1‑150 (MQ=60)
                                                                                                              TCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCAC                                        >  SRR19973067.1062289/1‑150 (MQ=60)
                                                                                                              TCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAAACAC                                        >  SRR19973067.1061446/1‑150 (MQ=60)
                                                                                                                 GCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTAC                                     <  SRR19973067.169176/150‑1 (MQ=60)
                                                                                                                 GCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTAC                                     <  SRR19973067.169149/150‑1 (MQ=60)
                                                                                                                   AATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACAC                                   <  SRR19973067.3196779/150‑1 (MQ=60)
                                                                                                                   AATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACAACAC                                   >  SRR19973067.2623104/1‑150 (MQ=60)
                                                                                                                    ATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACC                                  >  SRR19973067.2232006/1‑150 (MQ=60)
                                                                                                                    ATGGCGCTTTCTGTTCTGGTGGCATTGATTATTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATAACACCTTCGATCATATCGTTAAACACTACACC                                  >  SRR19973067.639126/1‑150 (MQ=60)
                                                                                                                     TGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCA                                 >  SRR19973067.388802/1‑150 (MQ=60)
                                                                                                                     TGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCA                                 >  SRR19973067.388810/1‑150 (MQ=60)
                                                                                                                        CGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACA                              <  SRR19973067.3249265/150‑1 (MQ=60)
                                                                                                                         GCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAG                             >  SRR19973067.3279763/1‑150 (MQ=60)
                                                                                                                              CTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCG                        <  SRR19973067.2462771/150‑1 (MQ=60)
                                                                                                                              CTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCG                        <  SRR19973067.2462795/150‑1 (MQ=60)
                                                                                                                               TGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGG                       >  SRR19973067.2896181/1‑150 (MQ=60)
                                                                                                                                   CTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAA                   >  SRR19973067.1773751/1‑150 (MQ=60)
                                                                                                                                   CTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAA                   >  SRR19973067.1773759/1‑150 (MQ=60)
                                                                                                                                       TGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCC               >  SRR19973067.3398804/1‑150 (MQ=60)
                                                                                                                                        GGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCT              >  SRR19973067.2989414/1‑150 (MQ=60)
                                                                                                                                         GCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTC             >  SRR19973067.2724041/1‑150 (MQ=60)
                                                                                                                                           ATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGG           >  SRR19973067.614999/1‑150 (MQ=60)
                                                                                                                                           ATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGG           >  SRR19973067.615880/1‑150 (MQ=60)
                                                                                                                                             TGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGAT         >  SRR19973067.681014/1‑150 (MQ=60)
                                                                                                                                             TGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCATCGGAT         <  SRR19973067.1978768/150‑1 (MQ=60)
                                                                                                                                                 TCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCAC     <  SRR19973067.183107/150‑1 (MQ=60)
                                                                                                                                                 TCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCAC     <  SRR19973067.1991175/150‑1 (MQ=60)
                                                                                                                                                   TTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCACAG   <  SRR19973067.2784059/150‑1 (MQ=60)
                                                                                                                                                   TTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCACAG   <  SRR19973067.997102/150‑1 (MQ=60)
                                                                                                                                                    TCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCACAGG  >  SRR19973067.480154/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                    
AGGGCGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCACAGG  >  CP009273/3409707‑3410004

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑