Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F394 I0 R1 1342 215.0 7134094 96.2% 6862998 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,409,856 A→C 100% T489P (ACC→CCC)  acrF → multidrug efflux system protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,409,8560AC98.3% 700.6 / ‑1.8 184T489P (ACC→CCC) acrFmultidrug efflux system protein
Reads supporting (aligned to +/- strand):  ref base A (3/1);  new base C (101/79);  total (104/80)
Fisher's exact test for biased strand distribution p-value = 6.34e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 189 total aligned reads displayed.

CGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGAT  >  CP009273/3409711‑3409997
                                                                                                                                                 |                                                                                                                                             
cgcACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTccc                                                                                                                                             <  2:2641645/148‑1 (MQ=255)
 gcACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTcccc                                                                                                                                            <  1:2439076/148‑1 (MQ=255)
  cACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCcccc                                                                                                                                           >  1:2877679/1‑148 (MQ=255)
      ggtggGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCg                                                                                                                                       <  2:1124824/148‑1 (MQ=255)
       gtggGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGt                                                                                                                                      >  2:4319/1‑148 (MQ=255)
        tggGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGtt                                                                                                                                     <  2:2967752/148‑1 (MQ=255)
        tggGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGtt                                                                                                                                     <  2:2937262/148‑1 (MQ=255)
           gTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTAtg                                                                                                                                  >  1:1223050/1‑148 (MQ=255)
             aTCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTAtgtg                                                                                                                                >  1:3496017/1‑148 (MQ=255)
                gcgATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCaa                                                                                                                             <  2:2247970/148‑1 (MQ=255)
                gcgATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCaa                                                                                                                             <  2:2247541/148‑1 (MQ=255)
                  gATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACg                                                                                                                           <  2:2635171/148‑1 (MQ=255)
                  gATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACg                                                                                                                           <  1:2813355/148‑1 (MQ=255)
                   aTGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACgc                                                                                                                          >  1:2980038/1‑148 (MQ=255)
                    tGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACgct                                                                                                                         >  2:2411364/1‑148 (MQ=255)
                       tGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACgctgct                                                                                                                      <  1:769981/148‑1 (MQ=255)
                          tGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTaa                                                                                                                   >  2:2028090/1‑148 (MQ=255)
                           gTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTaaa                                                                                                                  <  2:2809489/148‑1 (MQ=255)
                                cGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGt                                                                                                             <  1:1442276/148‑1 (MQ=255)
                                   tatttattCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGtctc                                                                                                          >  1:774898/1‑148 (MQ=255)
                                   tatttattCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGtctc                                                                                                          >  2:2930734/1‑148 (MQ=255)
                                     tttattCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTctg                                                                                                        <  1:1849790/148‑1 (MQ=255)
                                      ttattCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCACTGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTctgc                                                                                                       >  1:3490602/1‑148 (MQ=255)
                                      ttattCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCATACCCCTGCGTTATGTGCAACGCTGATTAAACCCGTCTctgc                                                                                                       >  1:2865481/1‑148 (MQ=255)
                                               tGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCa                                                                                              <  1:386219/148‑1 (MQ=255)
                                               tGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCa                                                                                              <  1:927569/148‑1 (MQ=255)
                                                ggCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCAc                                                                                             >  2:1765086/1‑148 (MQ=255)
                                                    ttcttcGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGaaa                                                                                         >  1:1066951/1‑148 (MQ=255)
                                                    ttcttcGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACCaaa                                                                                         >  1:336231/1‑148 (MQ=255)
                                                     tcttcGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGaaaa                                                                                        <  2:2076857/148‑1 (MQ=255)
                                                     tcttcGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAAACCGTCTCTGCTGAGCATCACGaaaa                                                                                        >  2:544987/1‑148 (MQ=255)
                                                      cttcGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAAt                                                                                       <  2:1586381/148‑1 (MQ=255)
                                                         cggcggTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAg                                                                                    <  2:1311847/148‑1 (MQ=255)
                                                         cggcggTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAg                                                                                    <  2:665258/148‑1 (MQ=255)
                                                             ggTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCg                                                                                >  1:2679764/1‑148 (MQ=255)
                                                             ggTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCg                                                                                <  1:1727763/148‑1 (MQ=255)
                                                               ttCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGt                                                                              >  1:2494241/1‑148 (MQ=255)
                                                                 cTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGttt                                                                            <  2:245954/148‑1 (MQ=255)
                                                                   aCTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTttct                                                                          <  1:846603/148‑1 (MQ=255)
                                                                   aCTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTttct                                                                          <  1:2396504/148‑1 (MQ=255)
                                                                    cTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTttctt                                                                         >  2:2589797/1‑148 (MQ=255)
                                                                    cTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTttctt                                                                         >  2:1996917/1‑148 (MQ=255)
                                                                      ggggCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCg                                                                       <  2:3556939/148‑1 (MQ=255)
                                                                       gggCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCgg                                                                      >  2:2822025/1‑148 (MQ=255)
                                                                       gggCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCgg                                                                      >  1:230781/1‑148 (MQ=255)
                                                                       gggCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCgg                                                                      <  2:1504625/148‑1 (MQ=255)
                                                                          cAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCggttg                                                                   <  1:2335831/148‑1 (MQ=255)
                                                                            aTTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCggttggt                                                                 <  1:2028090/148‑1 (MQ=255)
                                                                                aTCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTaa                                                             >  2:885872/1‑148 (MQ=255)
                                                                                   gCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATac                                                          >  1:1817923/1‑148 (MQ=255)
                                                                                   gCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATac                                                          <  1:2188235/148‑1 (MQ=255)
                                                                                     cAGTTCTCTATCACCATCGTTTCGGCAATGGCGCGTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATacca                                                        >  1:3224711/1‑148 (MQ=255)
                                                                                      aGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATaccac                                                       <  1:1325252/148‑1 (MQ=255)
                                                                                       gTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATaccacc                                                      <  2:3526653/148‑1 (MQ=255)
                                                                                       gTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATaccacc                                                      <  1:2094573/148‑1 (MQ=255)
                                                                                       gTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATaccacc                                                      <  1:2177615/148‑1 (MQ=255)
                                                                                       gTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGATATGGTGGCATTTATTATTCACCCTGCGTTATGTGCAAAGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTAGGTTGGTTTAATaccacc                                                      >  1:119611/1‑148 (MQ=255)
                                                                                        ttCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCt                                                     >  2:2941730/1‑148 (MQ=255)
                                                                                         tctcTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCtt                                                    <  2:1357093/148‑1 (MQ=255)
                                                                                            ctATCACCATAGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGa                                                 >  2:177063/1‑148 (MQ=255)
                                                                                              aTCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATc                                               >  2:2155556/1‑148 (MQ=255)
                                                                                                cACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCAt                                             <  1:69982/148‑1 (MQ=255)
                                                                                                cACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCAt                                             >  1:1095263/1‑148 (MQ=255)
                                                                                                 aCCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATa                                            >  2:1634398/1‑148 (MQ=255)
                                                                                                  ccATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGATGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAg                                           >  1:802881/1‑148 (MQ=255)
                                                                                                    aTCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCg                                         >  1:3089996/1‑148 (MQ=255)
                                                                                                     tCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAATGGCGGTTTCTTCGGTTGGTTTAATAACACCTTCGATCATAGCGt                                        >  1:1617924/1‑148 (MQ=255)
                                                                                                     tCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGt                                        >  1:1677335/1‑148 (MQ=255)
                                                                                                     tCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGt                                        >  1:562348/1‑148 (MQ=255)
                                                                                                      cGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGtt                                       >  1:1017291/1‑148 (MQ=255)
                                                                                                      cGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGtt                                       >  1:1589315/1‑148 (MQ=255)
                                                                                                      cGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACCCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGtt                                       >  1:1017111/1‑148 (MQ=255)
                                                                                                         ttCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAAc                                    >  2:2485763/1‑148 (MQ=255)
                                                                                                         ttCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAAc                                    <  2:630011/148‑1 (MQ=255)
                                                                                                         ttCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAAc                                    >  1:3458343/1‑148 (MQ=255)
                                                                                                         ttCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAAc                                    >  1:3458756/1‑148 (MQ=255)
                                                                                                             gCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACt                                >  1:592192/1‑148 (MQ=255)
                                                                                                              cAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCAcaa                               >  1:3177450/1‑146 (MQ=255)
                                                                                                              cAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTa                               >  2:3269437/1‑148 (MQ=255)
                                                                                                               aaTGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTac                              >  2:463315/1‑148 (MQ=255)
                                                                                                                   gcgcTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCa                          >  2:702250/1‑148 (MQ=255)
                                                                                                                   gcgcTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCa                          <  2:1677335/148‑1 (MQ=255)
                                                                                                                    cgcTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCaa                         <  2:1618780/148‑1 (MQ=255)
                                                                                                                      cTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACa                       >  2:1229512/1‑148 (MQ=255)
                                                                                                                      cTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACa                       >  2:3008628/1‑148 (MQ=255)
                                                                                                                       tttCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAg                      >  2:773949/1‑148 (MQ=255)
                                                                                                                       tttCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAg                      >  2:8330/1‑148 (MQ=255)
                                                                                                                        ttctgttctgGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGc                     <  1:99759/148‑1 (MQ=255)
                                                                                                                         tctgttctgGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCg                    <  1:2930734/148‑1 (MQ=255)
                                                                                                                          ctgttctgGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGt                   <  2:1817923/148‑1 (MQ=255)
                                                                                                                              tctgGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGc               >  1:2614342/1‑148 (MQ=255)
                                                                                                                              tctgGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGc               >  2:922754/1‑148 (MQ=255)
                                                                                                                                 ggtggCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCaaa            >  1:1620536/1‑148 (MQ=255)
                                                                                                                                  gtggCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCaaaa           <  2:2550390/148‑1 (MQ=255)
                                                                                                                                   tggCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAAt          >  2:1286675/1‑148 (MQ=255)
                                                                                                                                    ggCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATc         >  2:3058203/1‑148 (MQ=255)
                                                                                                                                     gCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATcc        >  1:439120/1‑148 (MQ=255)
                                                                                                                                        ttGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCg     >  2:3230739/1‑148 (MQ=255)
                                                                                                                                         tGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCACAATCCTCgg    >  2:2740068/1‑148 (MQ=255)
                                                                                                                                          gATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGa   <  1:1937959/148‑1 (MQ=255)
                                                                                                                                                 |                                                                                                                                             
CGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGAT  >  CP009273/3409711‑3409997

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCAC  >  CP009273/3409710‑3410001
                                                                                                                                                  |                                                                                                                                                 
GCGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCC                                                                                                                                                <  SRR19973066.486906/150‑1 (MQ=60)
GCGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCC                                                                                                                                                <  SRR19973066.1908491/150‑1 (MQ=60)
 CGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCC                                                                                                                                               >  SRR19973066.2896961/1‑150 (MQ=60)
  GCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCT                                                                                                                                              <  SRR19973066.2256042/150‑1 (MQ=60)
  GCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCT                                                                                                                                              <  SRR19973066.2455689/150‑1 (MQ=60)
   CACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTG                                                                                                                                             >  SRR19973066.1665313/1‑150 (MQ=60)
       GGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTT                                                                                                                                         <  SRR19973066.62647/150‑1 (MQ=60)
          GGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATG                                                                                                                                      >  SRR19973066.1231614/1‑150 (MQ=60)
          GGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATG                                                                                                                                      >  SRR19973066.1231697/1‑150 (MQ=60)
            GTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTG                                                                                                                                    >  SRR19973066.3519325/1‑150 (MQ=60)
              ATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCA                                                                                                                                  >  SRR19973066.2340443/1‑150 (MQ=60)
                CGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAAC                                                                                                                                <  SRR19973066.2160885/150‑1 (MQ=60)
                  CGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGC                                                                                                                              >  SRR19973066.2999719/1‑150 (MQ=60)
                   GATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCT                                                                                                                             <  SRR19973066.788777/150‑1 (MQ=60)
                   GATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCT                                                                                                                             <  SRR19973066.2832275/150‑1 (MQ=60)
                   GATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCT                                                                                                                             <  SRR19973066.2913603/150‑1 (MQ=60)
                        TGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTA                                                                                                                        <  SRR19973066.775684/150‑1 (MQ=60)
                            GTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACC                                                                                                                    <  SRR19973066.2046001/150‑1 (MQ=60)
                               AGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGT                                                                                                                 >  SRR19973066.1039365/1‑150 (MQ=60)
                                 CGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCT                                                                                                               <  SRR19973066.1452541/150‑1 (MQ=60)
                                  GGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTC                                                                                                              >  SRR19973066.780627/1‑150 (MQ=60)
                                   GTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCT                                                                                                             >  SRR19973066.1916666/1‑150 (MQ=60)
                                     ATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCACTGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGC                                                                                                           >  SRR19973066.3513888/1‑150 (MQ=60)
                                     ATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCATACCCCTGCGTTATGTGCAACGCTGATTAAACCCGTCTCTGC                                                                                                           >  SRR19973066.2884712/1‑150 (MQ=60)
                                      TTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCT                                                                                                          <  SRR19973066.1863030/150‑1 (MQ=60)
                                      TTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCT                                                                                                          <  SRR19973066.3158735/150‑1 (MQ=60)
                                                TGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACG                                                                                                <  SRR19973066.389080/150‑1 (MQ=60)
                                                TGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACG                                                                                                <  SRR19973066.2385439/150‑1 (MQ=60)
                                                TGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACG                                                                                                <  SRR19973066.389278/150‑1 (MQ=60)
                                                TGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACG                                                                                                <  SRR19973066.389304/150‑1 (MQ=60)
                                                TGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACG                                                                                                <  SRR19973066.934282/150‑1 (MQ=60)
                                                TGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACG                                                                                                <  SRR19973066.934288/150‑1 (MQ=60)
                                                   CATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAA                                                                                             >  SRR19973066.1074774/1‑150 (MQ=60)
                                                   CATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACCAAA                                                                                             >  SRR19973066.338811/1‑150 (MQ=60)
                                                   CATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGGTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAA                                                                                             >  SRR19973066.7471/1‑150 (MQ=60)
                                                          CGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGG                                                                                      <  SRR19973066.2955556/150‑1 (MQ=60)
                                                            GCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCG                                                                                    >  SRR19973066.2697652/1‑150 (MQ=60)
                                                            GCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCG                                                                                    <  SRR19973066.827930/150‑1 (MQ=60)
                                                              GGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGT                                                                                  >  SRR19973066.2511244/1‑150 (MQ=60)
                                                              GGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGT                                                                                  <  SRR19973066.1740254/150‑1 (MQ=60)
                                                              GGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGT                                                                                  <  SRR19973066.3514160/150‑1 (MQ=60)
                                                                TTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTT                                                                                >  SRR19973066.2895878/1‑150 (MQ=60)
                                                                TTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTT                                                                                >  SRR19973066.3423286/1‑150 (MQ=60)
                                                                   TACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTT                                                                             <  SRR19973066.1133559/150‑1 (MQ=60)
                                                                    ACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTC                                                                            <  SRR19973066.2412654/150‑1 (MQ=60)
                                                                    ACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTC                                                                            <  SRR19973066.852739/150‑1 (MQ=60)
                                                                      TGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGG                                                                          >  SRR19973066.232615/1‑150 (MQ=60)
                                                                         GGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTG                                                                       >  SRR19973066.2523026/1‑150 (MQ=60)
                                                                         GGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTG                                                                       >  SRR19973066.1313883/1‑150 (MQ=60)
                                                                           CAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGT                                                                     <  SRR19973066.2351753/150‑1 (MQ=60)
                                                                             ATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTT                                                                   <  SRR19973066.241926/150‑1 (MQ=60)
                                                                             ATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTT                                                                   <  SRR19973066.2042262/150‑1 (MQ=60)
                                                                                  TCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATAC                                                              >  SRR19973066.1830875/1‑150 (MQ=60)
                                                                                  TCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATAC                                                              >  SRR19973066.3255537/1‑150 (MQ=60)
                                                                                    GCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCA                                                            <  SRR19973066.2203418/150‑1 (MQ=60)
                                                                                    GCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCGTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTACGGTTTGTTTAATACCA                                                            >  SRR19973066.3246033/1‑150 (MQ=60)
                                                                                     CCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCAC                                                           >  SRR19973066.3048049/1‑150 (MQ=60)
                                                                                      CAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGATATGGTGGCATTTATTATTCACCCTGCGTTATGTGCAAAGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTAGGTTGGTTTAATACCACC                                                          >  SRR19973066.120822/1‑150 (MQ=60)
                                                                                       AGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCT                                                         <  SRR19973066.1334854/150‑1 (MQ=60)
                                                                                        GTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTT                                                        <  SRR19973066.2109001/150‑1 (MQ=60)
                                                                                        GTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTT                                                        <  SRR19973066.2192733/150‑1 (MQ=60)
                                                                                         TTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTC                                                       >  SRR19973066.2673565/1‑150 (MQ=60)
                                                                                           CTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGA                                                     >  SRR19973066.1960552/1‑150 (MQ=60)
                                                                                             CTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATC                                                   <  SRR19973066.1722655/150‑1 (MQ=60)
                                                                                               ATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCAT                                                 >  SRR19973066.1103222/1‑150 (MQ=60)
                                                                                               ATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCAT                                                 >  SRR19973066.1103230/1‑150 (MQ=60)
                                                                                                 CACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAG                                               <  SRR19973066.70829/150‑1 (MQ=60)
                                                                                                 CACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGATGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAG                                               >  SRR19973066.808780/1‑150 (MQ=60)
                                                                                                   CCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCG                                             >  SRR19973066.3110638/1‑150 (MQ=60)
                                                                                                    CATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAATGGCGGTTTCTTCGGTTGGTTTAATAACACCTTCGATCATAGCGT                                            >  SRR19973066.1629659/1‑150 (MQ=60)
                                                                                                    CATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATATCGT                                            >  SRR19973066.1628554/1‑150 (MQ=60)
                                                                                                    CATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGT                                            >  SRR19973066.566364/1‑150 (MQ=60)
                                                                                                    CATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGT                                            >  SRR19973066.566356/1‑150 (MQ=60)
                                                                                                    CATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAAACCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGT                                            >  SRR19973066.1689551/1‑150 (MQ=60)
                                                                                                     ATCGTTTCGGCAATGGCGCTTTGTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTT                                           >  SRR19973066.2830567/1‑150 (MQ=60)
                                                                                                     ATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTT                                           >  SRR19973066.1024778/1‑150 (MQ=60)
                                                                                                     ATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGGTTAATACCACCTTCGATCATAGCGTT                                           >  SRR19973066.1600820/1‑150 (MQ=60)
                                                                                                     ATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACCCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCATAGATCATAGCGTT                                           >  SRR19973066.1024597/1‑150 (MQ=60)
                                                                                                        GTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAAC                                        >  SRR19973066.3481900/1‑150 (MQ=60)
                                                                                                        GTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAAC                                        >  SRR19973066.3481885/1‑150 (MQ=60)
                                                                                                        GTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAAC                                        >  SRR19973066.3481467/1‑150 (MQ=60)
                                                                                                         TTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACC                                       <  SRR19973066.2490911/150‑1 (MQ=60)
                                                                                                         TTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACC                                       >  SRR19973066.1785444/1‑150 (MQ=60)
                                                                                                            CGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACT                                    >  SRR19973066.596300/1‑150 (MQ=60)
                                                                                                             GGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTA                                   >  SRR19973066.3198661/1‑150 (MQ=60)
                                                                                                             GGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCATCCATCATAGCGTTAACCACAA                                   >  SRR19973066.3198596/1‑150 (MQ=60)
                                                                                                                    GCGCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAAC                            <  SRR19973066.930360/150‑1 (MQ=60)
                                                                                                                      GCTTTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAG                          <  SRR19973066.1477973/150‑1 (MQ=60)
                                                                                                                         TTCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGT                       <  SRR19973066.100822/150‑1 (MQ=60)
                                                                                                                          TCTGTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTC                      <  SRR19973066.2950242/150‑1 (MQ=60)
                                                                                                                            TGTTCTGGTGGCATTGATTCTACCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGG                    >  SRR19973066.1402702/1‑150 (MQ=60)
                                                                                                                             GTTCTGGTGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGC                   >  SRR19973066.2632009/1‑150 (MQ=60)
                                                                                                                                CTGGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAA                >  SRR19973066.1632286/1‑150 (MQ=60)
                                                                                                                                    TGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCC            >  SRR19973066.1832465/1‑150 (MQ=60)
                                                                                                                                    TGGCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCC            >  SRR19973066.442516/1‑150 (MQ=60)
                                                                                                                                      GCATTGATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTC          <  SRR19973066.466856/150‑1 (MQ=60)
                                                                                                                                           GATTCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATC     <  SRR19973066.1951708/150‑1 (MQ=60)
                                                                                                                                              TCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCAC  >  SRR19973066.682900/1‑150 (MQ=60)
                                                                                                                                              TCTTCCCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCAC  >  SRR19973066.1104775/1‑150 (MQ=60)
                                                                                                                                                  |                                                                                                                                                 
GCGCACTGGTGGGTATCGCGATGGTGCTGTCAGCGGTATTTATTCCGATGGCATTCTTCGGCGGTTCTACTGGGGCAATTTATCGCCAGTTCTCTATCACCATCGTTTCGGCAATGGCGCTTTCTGTTCTGGTGGCATTGATTCTTACCCCTGCGTTATGTGCAACGCTGCTTAAACCCGTCTCTGCTGAGCATCACGAAAATAAGGGCGGTTTCTTCGGTTGGTTTAATACCACCTTCGATCATAGCGTTAACCACTACACCAACAGCGTCGGCAAAATCCTCGGATCCAC  >  CP009273/3409710‑3410001

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑