Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F247 I0 R1 1496 299.8 9582018 97.8% 9371213 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 743,797 G→T 88.1% R214R (CGC→CGA)  ybgO ← putative fimbrial protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273743,7970GT88.1% 627.3 / 78.7 254R214R (CGC→CGA) ybgOputative fimbrial protein
Reads supporting (aligned to +/- strand):  ref base G (19/12);  new base T (95/125);  total (117/137)
Fisher's exact test for biased strand distribution p-value = 8.18e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 257 total aligned reads displayed.

GGTGCATTGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGA  >  CP009273/743650‑743942
                                                                                                                                                   |                                                                                                                                                 
ggTGCATTGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCAcgc                                                                                                                                                  <  2:2395321/149‑1 (MQ=255)
 gTGCATTGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCActcg                                                                                                                                                 >  1:2732360/1‑149 (MQ=255)
   gCATTGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGaa                                                                                                                                               <  1:3498688/149‑1 (MQ=255)
     aTTGATCGTTTTGGCTCTTGGTCGTGTTAATACTGAACGTTTTCGACATTGTGTTGCGACGTATATCCAGCAAATTAAATTTAACAAAATAAAAAACCAGTGTTTCCGGTGTAATGGTAAAATTGACGCTGCAATCCAGCACTCGAATg                                                                                                                                             >  1:2036272/1‑149 (MQ=255)
       tGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGtt                                                                                                                                           <  1:4641492/149‑1 (MQ=255)
        gATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTTACGCTGCAATCCAGCACTCGAATGttt                                                                                                                                          >  1:2347185/1‑149 (MQ=255)
          tCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTc                                                                                                                                        <  1:3689625/149‑1 (MQ=255)
          cgatcttGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTc                                                                                                                                        <  2:1866110/144‑1 (MQ=255)
           cGTTTTGGCTCTTGGTCGTTTTAATAAAGAACGTTTTCGACAATGTGTTGCGACGTATATACAGCAAATTAAATTTACCAAAAAAAAAAACCTGTGTTTACGGTGAAATTGAAAAAATTACGCTGCAAAACAGCACACGAATGttttac                                                                                                                                       >  1:1300802/1‑147 (MQ=255)
                 ggCTCTTGGTCGTTTTAATACATAACGTTTTAGACATTTTTTTGCGACGTAAATCCAGAAAATTAAATTTAACAAAAACAAAAACCAGTTTTTAAGGTTAAATTGAAAAAAAGACGCATCAATCCATCACTCGAAAGTTTTCCAGaaag                                                                                                                                 >  1:1866110/1‑146 (MQ=255)
                    tctTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTa                                                                                                                              <  2:2774509/149‑1 (MQ=255)
                     ctTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAACTCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTaa                                                                                                                             <  2:2729408/149‑1 (MQ=255)
                      ttGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAat                                                                                                                            <  1:2785893/149‑1 (MQ=255)
                      ttGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAat                                                                                                                            <  1:168884/149‑1 (MQ=255)
                       tGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAata                                                                                                                           <  1:368300/149‑1 (MQ=255)
                       tGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAata                                                                                                                           <  1:4137096/149‑1 (MQ=255)
                       tGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAata                                                                                                                           >  1:1584327/1‑149 (MQ=255)
                       tGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAata                                                                                                                           <  1:4137150/149‑1 (MQ=255)
                       tGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAata                                                                                                                           <  2:251687/149‑1 (MQ=255)
                        ggTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAatat                                                                                                                          <  2:465485/149‑1 (MQ=255)
                        ggTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAatat                                                                                                                          <  2:2857880/149‑1 (MQ=255)
                          tCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGa                                                                                                                        >  1:2668538/1‑149 (MQ=255)
                           cGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGat                                                                                                                       <  1:2518895/149‑1 (MQ=255)
                            gTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGata                                                                                                                      >  2:2201248/1‑149 (MQ=255)
                               ttAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATAttt                                                                                                                   >  1:3226030/1‑149 (MQ=255)
                                 aaTACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCa                                                                                                                 >  1:1315870/1‑149 (MQ=255)
                                  aTACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGGTATTTCAg                                                                                                                <  1:1487842/149‑1 (MQ=255)
                                  aTACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAg                                                                                                                <  2:615668/149‑1 (MQ=255)
                                   tACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAgg                                                                                                               <  1:53847/149‑1 (MQ=255)
                                    aCTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCGCCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGt                                                                                                              >  2:3498135/1‑149 (MQ=255)
                                    aCTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGt                                                                                                              >  1:1173840/1‑149 (MQ=255)
                                    aCTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGt                                                                                                              <  2:4366228/149‑1 (MQ=255)
                                     cTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGtt                                                                                                             <  1:1752908/149‑1 (MQ=255)
                                     cTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGtt                                                                                                             <  2:4291782/149‑1 (MQ=255)
                                     cTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGtt                                                                                                             <  2:3098292/149‑1 (MQ=255)
                                      tGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGttt                                                                                                            <  1:1896378/149‑1 (MQ=255)
                                        aaCGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTg                                                                                                          >  2:4226476/1‑149 (MQ=255)
                                         aCGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTgg                                                                                                         <  2:4772339/149‑1 (MQ=255)
                                            ttttCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCa                                                                                                      >  2:307594/1‑149 (MQ=255)
                                               tCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTa                                                                                                   <  1:3630693/149‑1 (MQ=255)
                                                cGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAg                                                                                                  <  1:826941/149‑1 (MQ=255)
                                                 gACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAgg                                                                                                 <  1:2383584/149‑1 (MQ=255)
                                                       gtgtgGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTc                                                                                           <  2:2282840/149‑1 (MQ=255)
                                                             cGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAAc                                                                                     <  2:3349950/149‑1 (MQ=255)
                                                                  tataTCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTAc                                                                                <  1:3086123/149‑1 (MQ=255)
                                                                  tataTCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTAc                                                                                <  1:3537107/149‑1 (MQ=255)
                                                                    tatCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCg                                                                              >  2:1048282/1‑149 (MQ=255)
                                                                     atCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCgg                                                                             >  2:1031511/1‑149 (MQ=255)
                                                                        cAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCAc                                                                          >  2:724958/1‑149 (MQ=255)
                                                                        cAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCAc                                                                          >  2:1653206/1‑149 (MQ=255)
                                                                         aGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCAcc                                                                         >  2:3611069/1‑149 (MQ=255)
                                                                            aaattaaattTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTc                                                                      <  2:2697976/149‑1 (MQ=255)
                                                                             aattaaattTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCa                                                                     >  2:4158197/1‑149 (MQ=255)
                                                                               ttaaattTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCaaa                                                                   <  2:4446219/149‑1 (MQ=255)
                                                                                 aaattTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAAtt                                                                 >  1:450969/1‑149 (MQ=255)
                                                                                  aattTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTg                                                                >  2:3829581/1‑149 (MQ=255)
                                                                                   attTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGc                                                               >  1:2503339/1‑149 (MQ=255)
                                                                                   attTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGc                                                               >  1:2607104/1‑149 (MQ=255)
                                                                                      tACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCacg                                                            >  2:2536914/1‑149 (MQ=255)
                                                                                       aCCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCacga                                                           <  2:1216748/149‑1 (MQ=255)
                                                                                        ccAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCacgac                                                          >  1:9130/1‑149 (MQ=255)
                                                                                          aaaaTCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCacgacta                                                        >  2:458397/1‑147 (MQ=255)
                                                                                          aaaaTCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCAcgacga                                                        <  1:1065641/149‑1 (MQ=255)
                                                                                             atcaatcaCCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGatat                                                     >  1:446973/1‑149 (MQ=255)
                                                                                                aatcaCCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGt                                                  <  1:1572451/149‑1 (MQ=255)
                                                                                                  tcaCCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCg                                                <  1:3733148/149‑1 (MQ=255)
                                                                                                  tcaCCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCg                                                >  1:479221/1‑149 (MQ=255)
                                                                                                   caCCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGc                                               >  2:1569097/1‑149 (MQ=255)
                                                                                                   caCCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGc                                               <  1:4621198/149‑1 (MQ=255)
                                                                                                   caCCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGc                                               >  2:2559125/1‑149 (MQ=255)
                                                                                                       tgtgTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAg                                           >  2:1742312/1‑149 (MQ=255)
                                                                                                        gtgtTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGc                                          <  2:2505154/149‑1 (MQ=255)
                                                                                                        gtgtTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGc                                          >  2:3285737/1‑149 (MQ=255)
                                                                                                         tgtTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCg                                         <  1:719537/149‑1 (MQ=255)
                                                                                                          gtTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGt                                        >  1:316712/1‑149 (MQ=255)
                                                                                                           tttCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTa                                       <  1:206991/149‑1 (MQ=255)
                                                                                                            ttCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTCAGCGTAc                                      >  2:1508327/1‑149 (MQ=255)
                                                                                                            ttCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTAc                                      <  1:4250583/149‑1 (MQ=255)
                                                                                                                ggTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTg                                  >  2:1609972/1‑149 (MQ=255)
                                                                                                                   gAAATGTAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTAACGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATa                               >  2:1417897/1‑149 (MQ=255)
                                                                                                                   gAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCTCGACGATATAGTCGCTAAGCGTACTTTGATa                               <  2:4007064/149‑1 (MQ=255)
                                                                                                                   gAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATa                               >  1:4747361/1‑149 (MQ=255)
                                                                                                                       tGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCa                           <  1:291279/149‑1 (MQ=255)
                                                                                                                        ggAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCAt                          >  1:2571093/1‑149 (MQ=255)
                                                                                                                         gAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATg                         <  2:3982165/149‑1 (MQ=255)
                                                                                                                          aaaaaTTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATgc                        <  2:324601/149‑1 (MQ=255)
                                                                                                                            aaaTTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATgcgg                      <  2:862084/149‑1 (MQ=255)
                                                                                                                            aaaTTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATgcgg                      >  2:3693226/1‑149 (MQ=255)
                                                                                                                            aaaTTGACGCTGCAATCCAGCACTCGAATGTTGTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATgcgg                      <  2:2594904/149‑1 (MQ=255)
                                                                                                                             aaTTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATgcggc                     <  2:2383573/149‑1 (MQ=255)
                                                                                                                              aTTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATgcggcg                    >  1:2002445/1‑149 (MQ=255)
                                                                                                                              aTTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATgcggcg                    >  2:2024358/1‑149 (MQ=255)
                                                                                                                                   cGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCAtt               <  2:1315870/149‑1 (MQ=255)
                                                                                                                                    gCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCAttt              >  1:4392633/1‑149 (MQ=255)
                                                                                                                                    gCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCAttt              <  2:4623768/149‑1 (MQ=255)
                                                                                                                                     cTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTtc             >  1:2590374/1‑149 (MQ=255)
                                                                                                                                     cTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTtc             >  1:1619665/1‑149 (MQ=255)
                                                                                                                                           tCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTAt       >  1:3308566/1‑149 (MQ=255)
                                                                                                                                              aGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTAtttt    >  1:1293735/1‑149 (MQ=255)
                                                                                                                                                cACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGa  <  2:3185693/149‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                 
GGTGCATTGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGA  >  CP009273/743650‑743942

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GGTGCATTGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGT  >  CP009273/743650‑743945
                                                                                                                                                   |                                                                                                                                                    
GGTGCATTGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCG                                                                                                                                                    >  SRR19973071.2742067/1‑150 (MQ=60)
   GCATTGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAAT                                                                                                                                                 <  SRR19973071.3982413/150‑1 (MQ=60)
   GCATTGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAAT                                                                                                                                                 <  SRR19973071.1585511/150‑1 (MQ=60)
   GCATTGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAAT                                                                                                                                                 <  SRR19973071.3510980/150‑1 (MQ=60)
   GCATTGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAAT                                                                                                                                                 <  SRR19973071.3511187/150‑1 (MQ=60)
    CATTGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCAGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCCCTCGAATG                                                                                                                                                >  SRR19973071.2676606/1‑150 (MQ=60)
    CATTGATCGTTTTGGCTCTTGGTCGTTTTAATAATTAAAGTTTTAGACATTGTGTGGCGACGTATATACAGCAAAATAAATTTACCAAAATCAAACACCATTGTTTCCGGTGAAAATGAAAAATTTAAGCTGCAATCCAGCACGCGCATG                                                                                                                                                >  SRR19973071.3698416/1‑150 (MQ=60)
    CATTGATCGTTTTGGCTCTTGGTCGTTTTAATAATGAACGGTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTTACGCTGCAATCCAGCACGCGAATG                                                                                                                                                >  SRR19973071.2043722/1‑150 (MQ=60)
    CATTGATCGTTTTGGCTCTTGGTCGTGTTAATACTGAACGTTTTCGACATTGTGTTGCGACGTATATCCAGCAAATTAAATTTAACAAAATAAAAAACCAGTGTTTCCGGTGTAATGGTAAAATTGACGCTGCAATCCAGCACTCGAATG                                                                                                                                                >  SRR19973071.2043727/1‑150 (MQ=60)
     ATTGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGT                                                                                                                                               <  SRR19973071.1076411/150‑1 (MQ=60)
      TTGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTT                                                                                                                                              <  SRR19973071.880959/150‑1 (MQ=60)
       TGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTTACGCTGCAATCCAGCACTCGAATGTTT                                                                                                                                             >  SRR19973071.2355705/1‑150 (MQ=60)
       TGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTT                                                                                                                                             <  SRR19973071.4657992/150‑1 (MQ=60)
       TGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTT                                                                                                                                             <  SRR19973071.4658024/150‑1 (MQ=60)
          TCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCC                                                                                                                                          <  SRR19973071.3702897/150‑1 (MQ=60)
          TCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCC                                                                                                                                          <  SRR19973071.3702686/150‑1 (MQ=60)
          TCGTTTTGGCTCTTGGTCGTTTTAATAATGAACGTTTTAGACAATTTGTGGCGACGTATATACAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTACGGTGAAATGGAAAAAATGACGCTGCAATCCCGCACACGAATGTTTTAC                                                                                                                                          >  SRR19973071.1305628/1‑150 (MQ=60)
          TCGTTTTGGCTCTTGGTCGTTTTAATAATGAAAGTTTTAGAAATTGTGTGGCGACGTATATACAGCAAATTAAATTTAACAAAATAAATAACCTGTGTTTACGGTGAAATTGAAAAATTGAAGCTGCAATACAGCAATCGAAAGTTTTCC                                                                                                                                          >  SRR19973071.1305552/1‑150 (MQ=60)
          TCGTTTTGGCTCTTGGTCGTTTTAATAAAGAACGTTTTCGACAATGTGTTGCGACGTATATACAGCAAATTAAATTTACCAAAAAAAAAAACCTGTGTTTACGGTGAAATTGAAAAAATTACGCTGCAAAACAGCACACGAATGTTTTAC                                                                                                                                          >  SRR19973071.1305550/1‑150 (MQ=60)
           CGTTTTGGCTCTTGGTCGTGTTAATACTGAACGGTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCCCGCGAATTTTTTACA                                                                                                                                         >  SRR19973071.4640710/1‑150 (MQ=60)
               TTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACC                                                                                                                                     <  SRR19973071.2472759/150‑1 (MQ=60)
                TGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCA                                                                                                                                    <  SRR19973071.3098368/150‑1 (MQ=60)
                 GGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAG                                                                                                                                   <  SRR19973071.2327343/150‑1 (MQ=60)
                     CTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAAT                                                                                                                               <  SRR19973071.2502769/150‑1 (MQ=60)
                      TTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATA                                                                                                                              >  SRR19973071.1589938/1‑150 (MQ=60)
                      TTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATA                                                                                                                              <  SRR19973071.2795577/150‑1 (MQ=60)
                      TTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATA                                                                                                                              <  SRR19973071.2795675/150‑1 (MQ=60)
                      TTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATA                                                                                                                              <  SRR19973071.169410/150‑1 (MQ=60)
                      TTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATA                                                                                                                              <  SRR19973071.169609/150‑1 (MQ=60)
                       TGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATAT                                                                                                                             <  SRR19973071.369479/150‑1 (MQ=60)
                       TGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATAT                                                                                                                             <  SRR19973071.4151826/150‑1 (MQ=60)
                       TGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATAT                                                                                                                             <  SRR19973071.4151837/150‑1 (MQ=60)
                       TGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATAT                                                                                                                             <  SRR19973071.4151880/150‑1 (MQ=60)
                       TGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATAT                                                                                                                             <  SRR19973071.369470/150‑1 (MQ=60)
                       TGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATAT                                                                                                                             <  SRR19973071.369367/150‑1 (MQ=60)
                        GGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATG                                                                                                                            <  SRR19973071.4668886/150‑1 (MQ=60)
                         GTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGA                                                                                                                           >  SRR19973071.2678105/1‑150 (MQ=60)
                           CGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATA                                                                                                                         <  SRR19973071.2528063/150‑1 (MQ=60)
                           CGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATA                                                                                                                         <  SRR19973071.2528055/150‑1 (MQ=60)
                              TTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTT                                                                                                                      >  SRR19973071.3237107/1‑150 (MQ=60)
                              TTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTT                                                                                                                      >  SRR19973071.3237079/1‑150 (MQ=60)
                              TTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTT                                                                                                                      >  SRR19973071.3237368/1‑150 (MQ=60)
                              TTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTT                                                                                                                      >  SRR19973071.3295812/1‑150 (MQ=60)
                                TAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCA                                                                                                                    >  SRR19973071.1320696/1‑150 (MQ=60)
                                 AATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAG                                                                                                                   >  SRR19973071.2611149/1‑150 (MQ=60)
                                 AATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAG                                                                                                                   >  SRR19973071.2610694/1‑150 (MQ=60)
                                  ATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGGTATTTCAGG                                                                                                                  <  SRR19973071.1493242/150‑1 (MQ=60)
                                   TACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGT                                                                                                                 <  SRR19973071.54159/150‑1 (MQ=60)
                                   TACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGT                                                                                                                 <  SRR19973071.54107/150‑1 (MQ=60)
                                   TACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGT                                                                                                                 >  SRR19973071.1177887/1‑150 (MQ=60)
                                     CTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTT                                                                                                               <  SRR19973071.1759452/150‑1 (MQ=60)
                                      TGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTT                                                                                                              <  SRR19973071.2951799/150‑1 (MQ=60)
                                      TGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTT                                                                                                              <  SRR19973071.1903354/150‑1 (MQ=60)
                                      TGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTT                                                                                                              <  SRR19973071.1903366/150‑1 (MQ=60)
                                        AACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGG                                                                                                            <  SRR19973071.648077/150‑1 (MQ=60)
                                               TCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAG                                                                                                     <  SRR19973071.3643444/150‑1 (MQ=60)
                                               TCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAG                                                                                                     <  SRR19973071.3643437/150‑1 (MQ=60)
                                                CGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGG                                                                                                    <  SRR19973071.829809/150‑1 (MQ=60)
                                                 GACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGG                                                                                                   <  SRR19973071.2392174/150‑1 (MQ=60)
                                                 GACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGG                                                                                                   <  SRR19973071.2392123/150‑1 (MQ=60)
                                                    ATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCT                                                                                                <  SRR19973071.1373770/150‑1 (MQ=60)
                                                        TGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGT                                                                                            >  SRR19973071.1187611/1‑150 (MQ=60)
                                                                 GTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTAC                                                                                   >  SRR19973071.4489118/1‑150 (MQ=60)
                                                                  TATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACC                                                                                  <  SRR19973071.3097031/150‑1 (MQ=60)
                                                                  TATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACC                                                                                  <  SRR19973071.3549477/150‑1 (MQ=60)
                                                                       CCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCAC                                                                             >  SRR19973071.3360388/1‑150 (MQ=60)
                                                                        CAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACC                                                                            >  SRR19973071.2943150/1‑150 (MQ=60)
                                                                        CAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACC                                                                            <  SRR19973071.4804209/150‑1 (MQ=60)
                                                                            AAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCA                                                                        >  SRR19973071.2852849/1‑150 (MQ=60)
                                                                                TAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATT                                                                    >  SRR19973071.452591/1‑150 (MQ=60)
                                                                                 AAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCTGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTG                                                                   >  SRR19973071.3525913/1‑150 (MQ=60)
                                                                                  AATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGC                                                                  >  SRR19973071.2616618/1‑150 (MQ=60)
                                                                                  AATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGC                                                                  >  SRR19973071.2512461/1‑150 (MQ=60)
                                                                                  AATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGC                                                                  >  SRR19973071.2616529/1‑150 (MQ=60)
                                                                                    TTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCAC                                                                <  SRR19973071.4444945/150‑1 (MQ=60)
                                                                                       ACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGAC                                                             >  SRR19973071.9198/1‑150 (MQ=60)
                                                                                       ACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGAC                                                             >  SRR19973071.175577/1‑150 (MQ=60)
                                                                                         CAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGA                                                           >  SRR19973071.4775154/1‑150 (MQ=60)
                                                                                          AAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGAT                                                          <  SRR19973071.1069444/150‑1 (MQ=60)
                                                                                            AATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATAT                                                        >  SRR19973071.448572/1‑150 (MQ=60)
                                                                                                AATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTC                                                    <  SRR19973071.1578028/150‑1 (MQ=60)
                                                                                                 ATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCG                                                   >  SRR19973071.480987/1‑150 (MQ=60)
                                                                                                  TCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGC                                                  <  SRR19973071.3746189/150‑1 (MQ=60)
                                                                                                  TCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGC                                                  <  SRR19973071.3746395/150‑1 (MQ=60)
                                                                                                   CACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCT                                                 <  SRR19973071.4637672/150‑1 (MQ=60)
                                                                                                   CACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCT                                                 <  SRR19973071.4637565/150‑1 (MQ=60)
                                                                                                    ACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTA                                                >  SRR19973071.4400160/1‑150 (MQ=60)
                                                                                                       TGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGC                                             >  SRR19973071.409514/1‑150 (MQ=60)
                                                                                                         TGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGT                                           >  SRR19973071.552818/1‑150 (MQ=60)
                                                                                                         TGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGT                                           <  SRR19973071.2710425/150‑1 (MQ=60)
                                                                                                         TGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGT                                           <  SRR19973071.722041/150‑1 (MQ=60)
                                                                                                         TGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGT                                           <  SRR19973071.722085/150‑1 (MQ=60)
                                                                                                         TGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGT                                           <  SRR19973071.863775/150‑1 (MQ=60)
                                                                                                         TGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGT                                           <  SRR19973071.1890541/150‑1 (MQ=60)
                                                                                                         TGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGT                                           >  SRR19973071.317804/1‑150 (MQ=60)
                                                                                                           TTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTAC                                         <  SRR19973071.207756/150‑1 (MQ=60)
                                                                                                           TTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTAC                                         <  SRR19973071.207829/150‑1 (MQ=60)
                                                                                                           TTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTAC                                         <  SRR19973071.207883/150‑1 (MQ=60)
                                                                                                            TTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACT                                        <  SRR19973071.1867074/150‑1 (MQ=60)
                                                                                                            TTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACT                                        <  SRR19973071.4265675/150‑1 (MQ=60)
                                                                                                              CCGGTGAAATGGAAAAATTGACGCTTCAATCCAGCACTCGATTGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTTGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTT                                      <  SRR19973071.1686590/150‑1 (MQ=60)
                                                                                                                 GTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGAT                                   <  SRR19973071.1564560/150‑1 (MQ=60)
                                                                                                                 GTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGAT                                   <  SRR19973071.1564568/150‑1 (MQ=60)
                                                                                                                  TGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATA                                  >  SRR19973071.4764160/1‑150 (MQ=60)
                                                                                                                     AATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATC                               <  SRR19973071.2539713/150‑1 (MQ=60)
                                                                                                                     AATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATC                               <  SRR19973071.2539728/150‑1 (MQ=60)
                                                                                                                     AATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTATGATAATC                               <  SRR19973071.2769405/150‑1 (MQ=60)
                                                                                                                       TGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCAT                             >  SRR19973071.2580420/1‑150 (MQ=60)
                                                                                                                       TGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCAT                             <  SRR19973071.292329/150‑1 (MQ=60)
                                                                                                                       TGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCAT                             >  SRR19973071.2580476/1‑150 (MQ=60)
                                                                                                                        GGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATG                            >  SRR19973071.1607002/1‑150 (MQ=60)
                                                                                                                        GGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATG                            >  SRR19973071.1606894/1‑150 (MQ=60)
                                                                                                                           AAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTTATAATCATGCGG                         >  SRR19973071.3313621/1‑150 (MQ=60)
                                                                                                                             AATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCG                       >  SRR19973071.2009655/1‑150 (MQ=60)
                                                                                                                             AATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTATGATAATCATGCGGCG                       >  SRR19973071.4201653/1‑150 (MQ=60)
                                                                                                                                TGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCA                    >  SRR19973071.1913320/1‑150 (MQ=60)
                                                                                                                                   CGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTT                 >  SRR19973071.4408037/1‑150 (MQ=60)
                                                                                                                                    GCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTC                >  SRR19973071.2599761/1‑150 (MQ=60)
                                                                                                                                    GCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTC                >  SRR19973071.1625451/1‑150 (MQ=60)
                                                                                                                                          ATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTAT          <  SRR19973071.4465914/150‑1 (MQ=60)
                                                                                                                                          ATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTAT          >  SRR19973071.4152057/1‑150 (MQ=60)
                                                                                                                                          ATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTAT          >  SRR19973071.3320292/1‑150 (MQ=60)
                                                                                                                                            CCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTT        >  SRR19973071.1831320/1‑150 (MQ=60)
                                                                                                                                             CAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTT       >  SRR19973071.1298402/1‑150 (MQ=60)
                                                                                                                                             CAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTT       >  SRR19973071.1298474/1‑150 (MQ=60)
                                                                                                                                             CAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTT       >  SRR19973071.1298451/1‑150 (MQ=60)
                                                                                                                                                CACTCGAATGTTTTCCAGACCATTAATATGATATTTCTGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGAC    <  SRR19973071.460070/150‑1 (MQ=60)
                                                                                                                                                CACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGAC    >  SRR19973071.3185324/1‑150 (MQ=60)
                                                                                                                                                CACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGAC    >  SRR19973071.785207/1‑150 (MQ=60)
                                                                                                                                                 ACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACG   >  SRR19973071.694844/1‑150 (MQ=60)
                                                                                                                                                 ACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTAAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACG   >  SRR19973071.3094863/1‑150 (MQ=60)
                                                                                                                                                  CTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGT  >  SRR19973071.1438455/1‑150 (MQ=60)
                                                                                                                                                  CTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCATCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGT  >  SRR19973071.1843582/1‑150 (MQ=60)
                                                                                                                                                   |                                                                                                                                                    
GGTGCATTGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGT  >  CP009273/743650‑743945

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑