Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 743,797 G→T 100% R214R (CGC→CGA)  ybgO ← putative fimbrial protein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273743,7970GT97.8% 719.0 / 4.6 225R214R (CGC→CGA) ybgOputative fimbrial protein
Reads supporting (aligned to +/- strand):  ref base G (1/4);  new base T (98/120);  total (101/124)
Fisher's exact test for biased strand distribution p-value = 3.85e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.87e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 226 total aligned reads displayed.

GGTGCATTGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGAC  >  CP009273/743650‑743943
                                                                                                                                                   |                                                                                                                                                  
    cATTGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATg                                                                                                                                              >  2:1682156/1‑150 (MQ=255)
    cATTGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATg                                                                                                                                              <  1:1789018/150‑1 (MQ=255)
     aTTGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGt                                                                                                                                             >  2:726451/1‑150 (MQ=255)
     aTTGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGt                                                                                                                                             <  2:885357/150‑1 (MQ=255)
      ttGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGtt                                                                                                                                            >  1:112481/1‑150 (MQ=255)
        gATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGtttt                                                                                                                                          <  1:1457109/150‑1 (MQ=255)
        gATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGtttt                                                                                                                                          <  2:2334185/150‑1 (MQ=255)
         aTCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTc                                                                                                                                         <  2:2013992/150‑1 (MQ=255)
         aTCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTc                                                                                                                                         >  2:1256225/1‑150 (MQ=255)
           cGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCa                                                                                                                                       >  2:1737500/1‑150 (MQ=255)
                 ggCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAg                                                                                                                                 <  2:738522/150‑1 (MQ=255)
                     ctTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAat                                                                                                                             <  1:2150255/150‑1 (MQ=255)
                        ggTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATg                                                                                                                          <  1:1664889/150‑1 (MQ=255)
                         gTCGTTTTAATACTGAACGTTTTCGACATTTTGTGGCGACGTATATCCACCAAATTAAATTTACCAAAATCAATCAACTGTGTTTCCGGTGAAATGGAAAAAATTACTATGCAATCCAGCACACGAATCTTTTCCAGACCAGTAATATGa                                                                                                                         >  1:4109774/1‑150 (MQ=255)
                          tCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGat                                                                                                                        <  2:3004921/150‑1 (MQ=255)
                            gTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGatat                                                                                                                      >  1:3363129/1‑150 (MQ=255)
                                tAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCa                                                                                                                  >  2:631571/1‑150 (MQ=255)
                                 aaTACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAg                                                                                                                 >  2:1494425/1‑150 (MQ=255)
                                 aaTACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAg                                                                                                                 >  1:2658485/1‑150 (MQ=255)
                                 aaTACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTGTCCAGACCAGTAATATGATAGTTCAg                                                                                                                 >  2:4734609/1‑150 (MQ=255)
                                   tACTGAACCTTAACTACAATGACTGGCGACGTATATCCAGCAAATAAAATTTACACAAATCAATCACCTGTGTTTCCGGTGAAATGCAAAAATTGACGCTGCAATCCAGCACACGAATGTTTTCCAGACCAGTAATATGATAGTTCAGGt                                                                                                               >  2:470773/1‑150 (MQ=255)
                                      tGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGtttt                                                                                                            <  2:904317/150‑1 (MQ=255)
                                      tGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGtttt                                                                                                            >  2:3320796/1‑150 (MQ=255)
                                      tGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGtttt                                                                                                            >  1:831624/1‑150 (MQ=255)
                                       gAACGTTTTCGACATTGTGTTGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCAGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTg                                                                                                           <  1:3219951/150‑1 (MQ=255)
                                       gAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTg                                                                                                           <  1:2594807/150‑1 (MQ=255)
                                          cGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGc                                                                                                        <  1:390107/150‑1 (MQ=255)
                                           gTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCa                                                                                                       >  1:2314526/1‑150 (MQ=255)
                                                cGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAgg                                                                                                  >  2:3653502/1‑150 (MQ=255)
                                                 taCATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAggg                                                                                                 >  1:1912338/2‑150 (MQ=255)
                                                  aCATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGt                                                                                                <  2:442209/150‑1 (MQ=255)
                                                  aCATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGt                                                                                                >  2:2587677/1‑150 (MQ=255)
                                                         gtgGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGtt                                                                                         <  1:220197/150‑1 (MQ=255)
                                                          tgGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTa                                                                                        <  1:4142473/150‑1 (MQ=255)
                                                           ggCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTaa                                                                                       <  1:2574568/150‑1 (MQ=255)
                                                            gCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAAc                                                                                      <  2:2181808/150‑1 (MQ=255)
                                                            gCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAAc                                                                                      <  2:4889527/150‑1 (MQ=255)
                                                             cGACGTATATCCAGCAAATTAAATTTACCAAATTCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGAAGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACg                                                                                     <  1:2128551/150‑1 (MQ=255)
                                                             cGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACg                                                                                     <  1:4339518/150‑1 (MQ=255)
                                                               aCGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCt                                                                                   <  2:2078113/150‑1 (MQ=255)
                                                               aCGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCt                                                                                   <  1:2777976/150‑1 (MQ=255)
                                                                cGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTa                                                                                  >  2:3987735/1‑150 (MQ=255)
                                                                cGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTa                                                                                  >  2:4322075/1‑150 (MQ=255)
                                                                cGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTa                                                                                  <  1:5094995/150‑1 (MQ=255)
                                                                  tataTCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTAcc                                                                                <  1:744918/150‑1 (MQ=255)
                                                                  tataTCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTAcc                                                                                <  2:537249/150‑1 (MQ=255)
                                                                  tataTCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTAcc                                                                                >  1:2857396/1‑150 (MQ=255)
                                                                   atatCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGTTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCg                                                                               >  2:2945365/1‑150 (MQ=255)
                                                                    tatCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCgg                                                                              <  1:1342164/150‑1 (MQ=255)
                                                                    tatCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCgg                                                                              >  1:4734696/1‑150 (MQ=255)
                                                                     atCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGc                                                                             >  1:3626445/1‑150 (MQ=255)
                                                                            aaattaaattTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCa                                                                      <  1:1935507/150‑1 (MQ=255)
                                                                                taaattTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAAtt                                                                  <  1:1597620/150‑1 (MQ=255)
                                                                                taaattTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAAtt                                                                  <  2:3243570/150‑1 (MQ=255)
                                                                                taaattTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAAtt                                                                  <  1:2048236/150‑1 (MQ=255)
                                                                                        ccAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCacgacg                                                          <  2:2854248/150‑1 (MQ=255)
                                                                                             atcaatcaCCTGTGTTTCAGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGAtata                                                     >  2:1222648/1‑150 (MQ=255)
                                                                                                aatcaCCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTc                                                  <  2:2674960/150‑1 (MQ=255)
                                                                                                aatcaCCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTc                                                  <  1:2357449/150‑1 (MQ=255)
                                                                                                 atcaCCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCg                                                 >  1:3852868/1‑150 (MQ=255)
                                                                                                   caCCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCt                                               <  2:662355/150‑1 (MQ=255)
                                                                                                      cTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAg                                            <  1:1979921/150‑1 (MQ=255)
                                                                                                      cTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAg                                            >  1:4968102/1‑150 (MQ=255)
                                                                                                        gtgtTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCg                                          >  2:4313028/1‑150 (MQ=255)
                                                                                                         tgtTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGt                                         >  2:2320970/1‑150 (MQ=255)
                                                                                                         tgtTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGt                                         <  2:157056/150‑1 (MQ=255)
                                                                                                          gtTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTa                                        >  1:3745779/1‑150 (MQ=255)
                                                                                                          gtTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTa                                        <  2:2809655/150‑1 (MQ=255)
                                                                                                            ttCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACt                                      <  1:4391988/150‑1 (MQ=255)
                                                                                                              ccGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACttt                                    <  1:1836511/150‑1 (MQ=255)
                                                                                                              ccGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACttt                                    >  1:3976420/1‑150 (MQ=255)
                                                                                                                    aaaTGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAAt                              >  1:3955481/1‑150 (MQ=255)
                                                                                                                     aaTGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATc                             <  2:4109774/150‑1 (MQ=255)
                                                                                                                      aTGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCa                            <  1:4749682/150‑1 (MQ=255)
                                                                                                                       tGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCAt                           <  2:1101100/150‑1 (MQ=255)
                                                                                                                        ggAAAAATTGACGCTGCAATCCAGCACTCGATTGTTTTCCAGACCAGTATTATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGATACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATTATCATg                          <  1:2966172/150‑1 (MQ=255)
                                                                                                                        ggAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATg                          <  1:2419158/150‑1 (MQ=255)
                                                                                                                          aaaaaTTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATgcg                        <  2:2691719/150‑1 (MQ=255)
                                                                                                                          aaaaaTTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATgcg                        <  2:106087/150‑1 (MQ=255)
                                                                                                                           aaaaTTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATgcgg                       >  1:2698102/1‑150 (MQ=255)
                                                                                                                           aaaaTTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATgcgg                       >  2:309247/1‑150 (MQ=255)
                                                                                                                           aaaaTTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATgcgg                       <  2:3613450/150‑1 (MQ=255)
                                                                                                                             aaTTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATgcggcg                     <  1:726451/150‑1 (MQ=255)
                                                                                                                              aTTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGcggcgg                    >  1:4896984/1‑150 (MQ=255)
                                                                                                                              aTTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGcggcgg                    >  2:3210987/1‑150 (MQ=255)
                                                                                                                               ttGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCggcggc                   <  2:2570792/150‑1 (MQ=255)
                                                                                                                                tGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCa                  <  2:2060726/150‑1 (MQ=255)
                                                                                                                                 gACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCAt                 >  2:3794745/1‑150 (MQ=255)
                                                                                                                                 gACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCAt                 >  2:4618110/1‑150 (MQ=255)
                                                                                                                                   cGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCAttt               >  2:2414116/1‑150 (MQ=255)
                                                                                                                                    gCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTtc              <  1:138259/150‑1 (MQ=255)
                                                                                                                                       gCAATCCAGGACTCGAATGTTTTCCAGTCCTGTAATATGATATTTCAGGTTTTGGGCAGTATGTTCTTCGTTAACTGTACAGGCACCGTCAAATTGCACGAGGATTTATTCGCTAAGCGTACTTTTATAATCATGCGGCGGCATTTCTCg           <  2:4279896/150‑1 (MQ=255)
                                                                                                                                       gCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCg           >  1:5002615/1‑150 (MQ=255)
                                                                                                                                         aaTCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTa         <  1:4593973/150‑1 (MQ=255)
                                                                                                                                           tCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTAAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGAGTACTATGATAATCATGCGGAGGCATTTCTCGTAtt       >  1:3108664/1‑150 (MQ=255)
                                                                                                                                            ccAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTAttt      <  1:3142868/150‑1 (MQ=255)
                                                                                                                                             cAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTAtttt     >  2:1981124/1‑150 (MQ=255)
                                                                                                                                             cAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTAtttt     >  2:4066852/1‑150 (MQ=255)
                                                                                                                                              aGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTg    >  1:4666091/1‑150 (MQ=255)
                                                                                                                                                cACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGAc  >  2:3654136/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
GGTGCATTGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGAC  >  CP009273/743650‑743943

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGGTGCATTGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTA  >  CP009273/743649‑743946
                                                                                                                                                    |                                                                                                                                                     
CGGTGCATTGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTC                                                                                                                                                      >  SRR19973063.225676/1‑150 (MQ=60)
    GCATTGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAAT                                                                                                                                                  <  SRR19973063.2379349/150‑1 (MQ=60)
    GCATTGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATACAGCAAAATAAATTTACCAAAATAAATCACCAGTGTTTCCGGTGAAATGGAAAAATTGACTCTGCAATCCAGCACTCGAAT                                                                                                                                                  >  SRR19973063.560849/1‑150 (MQ=60)
     CATTGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATG                                                                                                                                                 <  SRR19973063.1794550/150‑1 (MQ=60)
      ATTGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTTACGCTGCAATCCAGCACTCGAATGT                                                                                                                                                >  SRR19973063.3253987/1‑150 (MQ=60)
       TTGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTT                                                                                                                                               >  SRR19973063.112859/1‑150 (MQ=60)
         GATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTT                                                                                                                                             <  SRR19973063.1461551/150‑1 (MQ=60)
         GATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTT                                                                                                                                             <  SRR19973063.1461467/150‑1 (MQ=60)
            CGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCA                                                                                                                                          <  SRR19973063.3912090/150‑1 (MQ=60)
            CGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTACGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCA                                                                                                                                          >  SRR19973063.3067063/1‑150 (MQ=60)
             GTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAG                                                                                                                                         >  SRR19973063.4343665/1‑150 (MQ=60)
              TTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGA                                                                                                                                        <  SRR19973063.2905130/150‑1 (MQ=60)
                      CTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAAT                                                                                                                                <  SRR19973063.2156991/150‑1 (MQ=60)
                      CTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAAT                                                                                                                                <  SRR19973063.2888103/150‑1 (MQ=60)
                      CTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAAT                                                                                                                                <  SRR19973063.1546153/150‑1 (MQ=60)
                         GGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATG                                                                                                                             <  SRR19973063.1669966/150‑1 (MQ=60)
                         GGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATG                                                                                                                             <  SRR19973063.1669856/150‑1 (MQ=60)
                          GTCGTTTTAATACTGAACGTTTTCGACATTTTGTGGCGACGTATATCCACCAAATTAAATTTACCAAAATCAATCAACTGTGTTTCCGGTGAAATGGAAAAAATTACTATGCAATCCAGCACACGAATCTTTTCCAGACCAGTAATATGA                                                                                                                            >  SRR19973063.4122935/1‑150 (MQ=60)
                           TCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGAT                                                                                                                           <  SRR19973063.4884203/150‑1 (MQ=60)
                            CGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATA                                                                                                                          <  SRR19973063.5129889/150‑1 (MQ=60)
                            CGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATA                                                                                                                          <  SRR19973063.1560857/150‑1 (MQ=60)
                             GTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATAT                                                                                                                         <  SRR19973063.183123/150‑1 (MQ=60)
                             GTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATAT                                                                                                                         <  SRR19973063.829764/150‑1 (MQ=60)
                             GTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATAT                                                                                                                         >  SRR19973063.3373833/1‑150 (MQ=60)
                                  AATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAG                                                                                                                    >  SRR19973063.2666998/1‑150 (MQ=60)
                                       TGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTT                                                                                                               >  SRR19973063.834026/1‑150 (MQ=60)
                                        GAACGTTTTCGACATTGTGTTGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCAGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTG                                                                                                              <  SRR19973063.3230299/150‑1 (MQ=60)
                                        GAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTG                                                                                                              <  SRR19973063.2603052/150‑1 (MQ=60)
                                        GAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTG                                                                                                              <  SRR19973063.2603595/150‑1 (MQ=60)
                                        GAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCCGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTG                                                                                                              <  SRR19973063.3230174/150‑1 (MQ=60)
                                           CGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGC                                                                                                           <  SRR19973063.391215/150‑1 (MQ=60)
                                           CGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTACAGGTTTTGGGC                                                                                                           >  SRR19973063.5002445/1‑150 (MQ=60)
                                            GTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCA                                                                                                          >  SRR19973063.2321929/1‑150 (MQ=60)
                                            GTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCA                                                                                                          >  SRR19973063.1863681/1‑150 (MQ=60)
                                                 CGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGG                                                                                                     >  SRR19973063.4716696/1‑150 (MQ=60)
                                                  TACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGG                                                                                                    >  SRR19973063.1918322/1‑150 (MQ=60)
                                                  GACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCGGGTTTTGGGCAGTAGGG                                                                                                    <  SRR19973063.4600990/150‑1 (MQ=60)
                                                  GACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGG                                                                                                    <  SRR19973063.4600973/150‑1 (MQ=60)
                                                    CATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTC                                                                                                  <  SRR19973063.1373470/150‑1 (MQ=60)
                                                          GTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTT                                                                                            <  SRR19973063.220859/150‑1 (MQ=60)
                                                           TGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTA                                                                                           <  SRR19973063.4155725/150‑1 (MQ=60)
                                                            GGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAA                                                                                          <  SRR19973063.2582726/150‑1 (MQ=60)
                                                            GGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAA                                                                                          <  SRR19973063.2582761/150‑1 (MQ=60)
                                                              CGACGTATATCCAGCAAATTAAATTTACCAAATTCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGAAGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACG                                                                                        <  SRR19973063.2135198/150‑1 (MQ=60)
                                                              CGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACG                                                                                        <  SRR19973063.2135255/150‑1 (MQ=60)
                                                              CGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACG                                                                                        >  SRR19973063.4356924/1‑150 (MQ=60)
                                                              CGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACG                                                                                        <  SRR19973063.4353353/150‑1 (MQ=60)
                                                                ACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCT                                                                                      <  SRR19973063.2786923/150‑1 (MQ=60)
                                                                 CGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTA                                                                                     <  SRR19973063.5111388/150‑1 (MQ=60)
                                                                   TATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACC                                                                                   <  SRR19973063.747097/150‑1 (MQ=60)
                                                                   TATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACC                                                                                   >  SRR19973063.2866553/1‑150 (MQ=60)
                                                                   TATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACC                                                                                   >  SRR19973063.2705601/1‑150 (MQ=60)
                                                                    ATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCN                                                                                  <  SRR19973063.207843/150‑1 (MQ=60)
                                                                     TATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGG                                                                                 <  SRR19973063.1346192/150‑1 (MQ=60)
                                                                     TATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGG                                                                                 >  SRR19973063.4749780/1‑150 (MQ=60)
                                                                     TATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGG                                                                                 >  SRR19973063.7333/1‑150 (MQ=60)
                                                                      ATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGC                                                                                >  SRR19973063.3638086/1‑150 (MQ=60)
                                                                            CAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTC                                                                          >  SRR19973063.1779237/1‑150 (MQ=60)
                                                                             AAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATTTTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCA                                                                         <  SRR19973063.1941371/150‑1 (MQ=60)
                                                                             AAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCA                                                                         <  SRR19973063.1941558/150‑1 (MQ=60)
                                                                               ATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAA                                                                       >  SRR19973063.3919589/1‑150 (MQ=60)
                                                                                TTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAAT                                                                      <  SRR19973063.2122029/150‑1 (MQ=60)
                                                                                 TAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATT                                                                     <  SRR19973063.2054634/150‑1 (MQ=60)
                                                                                 TAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATT                                                                     <  SRR19973063.1602520/150‑1 (MQ=60)
                                                                                          CAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGA                                                            <  SRR19973063.2734581/150‑1 (MQ=60)
                                                                                           AAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGAT                                                           >  SRR19973063.5057905/1‑150 (MQ=60)
                                                                                             AATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATAT                                                         <  SRR19973063.2250747/150‑1 (MQ=60)
                                                                                                 AATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTC                                                     >  SRR19973063.970661/1‑150 (MQ=60)
                                                                                                 AATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTC                                                     <  SRR19973063.2364994/150‑1 (MQ=60)
                                                                                                  ATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCG                                                    >  SRR19973063.3865117/1‑150 (MQ=60)
                                                                                                    CACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCT                                                  >  SRR19973063.2326988/1‑150 (MQ=60)
                                                                                                    CACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCT                                                  >  SRR19973063.938093/1‑150 (MQ=60)
                                                                                                       CTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAG                                               >  SRR19973063.4984156/1‑150 (MQ=60)
                                                                                                       CTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAG                                               <  SRR19973063.1986113/150‑1 (MQ=60)
                                                                                                       CTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAG                                               <  SRR19973063.1986162/150‑1 (MQ=60)
                                                                                                       CTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAG                                               <  SRR19973063.4127519/150‑1 (MQ=60)
                                                                                                           GTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTA                                           <  SRR19973063.2356896/150‑1 (MQ=60)
                                                                                                           GTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTA                                           >  SRR19973063.3757825/1‑150 (MQ=60)
                                                                                                           GTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTA                                           >  SRR19973063.3757749/1‑150 (MQ=60)
                                                                                                             TTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACT                                         <  SRR19973063.4406052/150‑1 (MQ=60)
                                                                                                              TCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTT                                        >  SRR19973063.1980516/1‑150 (MQ=60)
                                                                                                               CCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTT                                       <  SRR19973063.1842236/150‑1 (MQ=60)
                                                                                                               CCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTT                                       >  SRR19973063.3989152/1‑150 (MQ=60)
                                                                                                               CCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTT                                       >  SRR19973063.3989161/1‑150 (MQ=60)
                                                                                                                 GGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGA                                     >  SRR19973063.2178732/1‑150 (MQ=60)
                                                                                                                 GGTGAAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGA                                     >  SRR19973063.4751307/1‑150 (MQ=60)
                                                                                                                     AAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAAT                                 >  SRR19973063.3968137/1‑150 (MQ=60)
                                                                                                                     AAATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAAT                                 <  SRR19973063.4624370/150‑1 (MQ=60)
                                                                                                                      AATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATC                                >  SRR19973063.3659051/1‑150 (MQ=60)
                                                                                                                       ATGGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCA                               <  SRR19973063.4764812/150‑1 (MQ=60)
                                                                                                                         GGAAAAATTGACGCTGCAATCCAGCACTCGATTGTTTTCCAGACCAGTATTATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGATACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATTATCATG                             <  SRR19973063.2975654/150‑1 (MQ=60)
                                                                                                                         GGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATG                             <  SRR19973063.4019633/150‑1 (MQ=60)
                                                                                                                         GGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATG                             <  SRR19973063.2426892/150‑1 (MQ=60)
                                                                                                                         GGAAAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATG                             <  SRR19973063.4019687/150‑1 (MQ=60)
                                                                                                                            AAAATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGG                          >  SRR19973063.2706803/1‑150 (MQ=60)
                                                                                                                              AATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCG                        <  SRR19973063.728588/150‑1 (MQ=60)
                                                                                                                              AATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCG                        >  SRR19973063.2807024/1‑150 (MQ=60)
                                                                                                                               ATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGG                       >  SRR19973063.4912788/1‑150 (MQ=60)
                                                                                                                               ATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGG                       >  SRR19973063.4912789/1‑150 (MQ=60)
                                                                                                                               ATTGACGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGG                       >  SRR19973063.4912997/1‑150 (MQ=60)
                                                                                                                                    CGCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTT                  <  SRR19973063.2634356/150‑1 (MQ=60)
                                                                                                                                     GCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTC                 >  SRR19973063.1906694/1‑150 (MQ=60)
                                                                                                                                     GCTGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTC                 <  SRR19973063.138694/150‑1 (MQ=60)
                                                                                                                                       TGCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTC               >  SRR19973063.1130446/1‑150 (MQ=60)
                                                                                                                                        GCAATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCG              >  SRR19973063.5018769/1‑150 (MQ=60)
                                                                                                                                          AATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTA            <  SRR19973063.4608673/150‑1 (MQ=60)
                                                                                                                                          AATCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTA            <  SRR19973063.2595904/150‑1 (MQ=60)
                                                                                                                                            TCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATT          >  SRR19973063.3798044/1‑150 (MQ=60)
                                                                                                                                            TCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATT          >  SRR19973063.3118804/1‑150 (MQ=60)
                                                                                                                                            TCCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTAAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGAGTACTATGATAATCATGCGGAGGCATTTCTCGTATT          >  SRR19973063.3118668/1‑150 (MQ=60)
                                                                                                                                             CCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTT         <  SRR19973063.3152995/150‑1 (MQ=60)
                                                                                                                                             CCAGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTT         <  SRR19973063.3152973/150‑1 (MQ=60)
                                                                                                                                               AGCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTG       >  SRR19973063.4680975/1‑150 (MQ=60)
                                                                                                                                                GCACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGA      >  SRR19973063.3496576/1‑150 (MQ=60)
                                                                                                                                                  ACTCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACG    >  SRR19973063.5177239/1‑150 (MQ=60)
                                                                                                                                                    TCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTA  >  SRR19973063.3257311/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
CGGTGCATTGATCGTTTTGGCTCTTGGTCGTTTTAATACTGAACGTTTTCGACATTGTGTGGCGACGTATATCCAGCAAATTAAATTTACCAAAATCAATCACCTGTGTTTCCGGTGAAATGGAAAAATTGACGCTGCAATCCAGCACGCGAATGTTTTCCAGACCAGTAATATGATATTTCAGGTTTTGGGCAGTAGGGTCTTCGTTAACGCTACCGGCACCGTCAAATTGCACGACGATATAGTCGCTAAGCGTACTTTGATAATCATGCGGCGGCATTTCTCGTATTTTGACGTA  >  CP009273/743649‑743946

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑