Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 321,223 C→A 100% intergenic (‑148/+65) ykgH ← / ← betA putative inner membrane protein/choline dehydrogenase, a flavoprotein

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273321,2230CA96.7% 710.8 / 13.6 214intergenic (‑148/+65)ykgH/betAputative inner membrane protein/choline dehydrogenase, a flavoprotein
Reads supporting (aligned to +/- strand):  ref base C (2/5);  new base A (110/97);  total (112/102)
Fisher's exact test for biased strand distribution p-value = 2.62e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.92e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 214 total aligned reads displayed.

ATATATCCAGAGAATAAAATCTGTCGCAGATAAGGTTGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTCGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGCTCCTCGGCAGCGCTTCCTGTCCACGAAT  >  CP009273/321077‑321368
                                                                                                                                                  |                                                                                                                                                 
atatatCCAGAGAATAAAATCTGTCGCAGATAAGGTTGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGt                                                                                                                                                <  1:4109424/150‑1 (MQ=255)
 tatatCCAGAGAATAAAATCTGTCGCAGATAAGGTTGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTc                                                                                                                                               >  2:2083122/1‑150 (MQ=255)
 tatatCCAGAGAATAAAATCTGTCGCAGATAAGGTTGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTc                                                                                                                                               >  1:1738691/1‑150 (MQ=255)
  atatCCAGAGAATAAAATCTGTCGCAGATAAGGTTGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTcc                                                                                                                                              >  2:2531494/1‑150 (MQ=255)
   tatCCAGAGAATAAAATCTGTCGCAGATAAGGTTGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTccc                                                                                                                                             <  1:3698692/150‑1 (MQ=255)
    atCCAGAGAATAAAATCTGTCGCAGATAAGNTTGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTcccc                                                                                                                                            >  1:4277923/1‑150 (MQ=255)
           gaATAAAATCTGTCGCAGATAAGGTTGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTg                                                                                                                                     >  2:707830/1‑150 (MQ=255)
             aTAAAATCTGTCGCAGATAAGGTTGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGa                                                                                                                                   >  2:639890/1‑150 (MQ=255)
             aTAAAATCTGTCGCAGATAAGGTTGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGa                                                                                                                                   >  2:2316409/1‑150 (MQ=255)
                    cTGTCGCAGATAAGGTTGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTaa                                                                                                                            <  2:3257859/150‑1 (MQ=255)
                        cGCAGATAAGGTTGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAActct                                                                                                                        >  2:1881444/1‑150 (MQ=255)
                          cAGATAAGGTTGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTCGGTCCCCCTCTCTGGAACGGTAACTCTCa                                                                                                                      >  1:751395/1‑150 (MQ=255)
                           aGATAAGGTTGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCaa                                                                                                                     >  2:49011/1‑150 (MQ=255)
                              tAAGTTTTTATTAATAGTCTGTATCAGTAATGTTCTGGTTAAATATCAGCAATAAGCCCGCATCATGAATACTGTATATGAAGCATGATAGTTACGTCAGTGTTTATATAATGATTAGGTACCGCTCTCTGGATCGGTAACTCTCAATCt                                                                                                                  <  1:2190094/150‑1 (MQ=255)
                                 ggTTGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGAt                                                                                                               >  1:563544/1‑150 (MQ=255)
                                    tGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCgg                                                                                                            >  1:3189865/1‑150 (MQ=255)
                                    tGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCgg                                                                                                            >  1:1480135/1‑150 (MQ=255)
                                     gTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGt                                                                                                           >  1:1928774/1‑150 (MQ=255)
                                     gTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGt                                                                                                           >  2:4584953/1‑150 (MQ=255)
                                       aTTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTc                                                                                                         >  2:48468/1‑150 (MQ=255)
                                       aTTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTc                                                                                                         >  1:1352463/1‑150 (MQ=255)
                                        ttAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTcc                                                                                                        <  2:41291/150‑1 (MQ=255)
                                         tAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCt                                                                                                       <  2:3595583/150‑1 (MQ=255)
                                          aaTAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTg                                                                                                      <  1:3897239/150‑1 (MQ=255)
                                          aaTAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTg                                                                                                      >  2:395385/1‑150 (MQ=255)
                                          aaTAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTg                                                                                                      >  2:3362172/1‑150 (MQ=255)
                                           aTAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTCGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGc                                                                                                     <  1:4560213/150‑1 (MQ=255)
                                              gTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGGTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGtt                                                                                                  <  2:4280622/150‑1 (MQ=255)
                                                cTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAg                                                                                                <  2:1697808/150‑1 (MQ=255)
                                                cTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAg                                                                                                <  1:1013443/150‑1 (MQ=255)
                                                cTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAg                                                                                                >  2:386907/1‑150 (MQ=255)
                                                    aTCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTca                                                                                            >  1:3384170/1‑150 (MQ=255)
                                                       aGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACAt                                                                                         <  2:3965953/150‑1 (MQ=255)
                                                       aGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCACAATCAGATCGGTTCCTGCGTTAGTTCACAt                                                                                         >  1:1389052/1‑150 (MQ=255)
                                                        ggAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATc                                                                                        <  2:4306156/150‑1 (MQ=255)
                                                          aaTGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCAc                                                                                      <  1:277858/150‑1 (MQ=255)
                                                            tGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGa                                                                                    >  1:432309/1‑150 (MQ=255)
                                                              ttCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACt                                                                                  >  1:1828755/1‑150 (MQ=255)
                                                               tCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTc                                                                                 <  1:3304133/150‑1 (MQ=255)
                                                                cGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCa                                                                                <  1:2068080/150‑1 (MQ=255)
                                                                 gggTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCAt                                                                               <  1:2682155/150‑1 (MQ=255)
                                                                    ttAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTCGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCAtttt                                                                            <  1:3096690/150‑1 (MQ=255)
                                                                    ttAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCAtttt                                                                            <  2:3586013/150‑1 (MQ=255)
                                                                        atatCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCg                                                                        >  2:286331/1‑150 (MQ=255)
                                                                        atatCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCg                                                                        >  2:2639664/1‑150 (MQ=255)
                                                                            cAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGctct                                                                    <  1:718858/150‑1 (MQ=255)
                                                                             aGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCtctc                                                                   <  1:1414604/150‑1 (MQ=255)
                                                                             aGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCtctc                                                                   <  2:1807385/150‑1 (MQ=255)
                                                                              gCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCa                                                                  <  1:3394977/150‑1 (MQ=255)
                                                                                aaaaaGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCAcc                                                                <  1:2536658/150‑1 (MQ=255)
                                                                                aaaaaGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCAcc                                                                >  1:4189189/1‑150 (MQ=255)
                                                                                aaaaaGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCAcc                                                                >  2:3097220/1‑150 (MQ=255)
                                                                                aaaaaGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCAcc                                                                <  2:4086383/150‑1 (MQ=255)
                                                                                 aaaaGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCg                                                               >  1:1143503/1‑150 (MQ=255)
                                                                                    aGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGACCGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCa                                                            >  2:3801293/1‑150 (MQ=255)
                                                                                    aGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCa                                                            >  1:4500237/1‑150 (MQ=255)
                                                                                       ccGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATcc                                                         <  1:845117/150‑1 (MQ=255)
                                                                                         gCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCa                                                       >  1:102988/1‑150 (MQ=255)
                                                                                          catcatGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCAt                                                      >  1:1651366/1‑150 (MQ=255)
                                                                                              atGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGc                                                  <  1:4219177/150‑1 (MQ=255)
                                                                                                gAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCca                                                <  1:3407063/150‑1 (MQ=255)
                                                                                                 aaTACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCcac                                               >  1:1532950/1‑150 (MQ=255)
                                                                                                 aaTACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCcac                                               >  2:2403264/1‑150 (MQ=255)
                                                                                                    aCTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACaaa                                            >  1:1533377/1‑150 (MQ=255)
                                                                                                     cTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACaaaa                                           <  1:799777/150‑1 (MQ=255)
                                                                                                     cTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACaaaa                                           >  1:627843/1‑150 (MQ=255)
                                                                                                     cTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACaaaa                                           >  2:2756006/1‑150 (MQ=255)
                                                                                                     cTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACAACACGACTCATTTTTTCGCTCTCACCGGCATCCCATATGCCACaaaa                                           >  2:1089812/1‑150 (MQ=255)
                                                                                                       ggATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAata                                         >  1:1986181/1‑150 (MQ=255)
                                                                                                       ggATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAata                                         >  1:3620473/1‑150 (MQ=255)
                                                                                                         atatGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATc                                       >  1:2957467/1‑150 (MQ=255)
                                                                                                         atatGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATc                                       <  2:2808594/150‑1 (MQ=255)
                                                                                                         atatGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATc                                       <  2:1112555/150‑1 (MQ=255)
                                                                                                          tatGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATcc                                      >  1:3982487/1‑150 (MQ=255)
                                                                                                             gAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCccgc                                   >  2:290339/1‑150 (MQ=255)
                                                                                                              aaGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCccgcc                                  <  1:3568624/150‑1 (MQ=255)
                                                                                                               aGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCccgccg                                 >  2:3218907/1‑150 (MQ=255)
                                                                                                                gCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGt                                >  2:696256/1‑150 (MQ=255)
                                                                                                                 cATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAAGCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTg                               >  1:2901747/1‑150 (MQ=255)
                                                                                                                    gagaGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGctc                            >  2:383883/1‑150 (MQ=255)
                                                                                                                     agagTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGctcc                           >  2:2048938/1‑150 (MQ=255)
                                                                                                                      gagTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGctcct                          >  1:4540401/1‑150 (MQ=255)
                                                                                                                       agTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGctcctc                         <  2:2051859/150‑1 (MQ=255)
                                                                                                                          tACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGCTCCTCGGc                      >  1:2984660/1‑150 (MQ=255)
                                                                                                                           aCCTCAGTGTTTATATTAGGATTAGGTCCACCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGCTCCTCGGCa                     <  2:4244551/150‑1 (MQ=255)
                                                                                                                           aCCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGCTCCTCGGCa                     <  2:2213011/150‑1 (MQ=255)
                                                                                                                                aGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGCTCCTCGGCAGCGCt                <  1:4530047/150‑1 (MQ=255)
                                                                                                                                 gtgtTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGCTCCTCGGCAGCGCtt               >  2:3716102/1‑150 (MQ=255)
                                                                                                                                 gtgtTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGCTCCTCGGCAGCGCtt               >  1:2867240/1‑150 (MQ=255)
                                                                                                                                 gtgtTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGCTCCTCGGCAGCGCtt               >  2:1396850/1‑150 (MQ=255)
                                                                                                                                  tgtTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGCTCCTCGGCAGCGCTTc              >  2:1516096/1‑150 (MQ=255)
                                                                                                                                  tgtTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGCTCCTCGGCAGCGCTTc              >  1:3688705/1‑150 (MQ=255)
                                                                                                                                   gtTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGCTCCTCGGCAGCGCTTcc             >  2:4576791/1‑150 (MQ=255)
                                                                                                                                       atataAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGCTCCTCGGCAGCGCTTCCTGTc         >  1:1437067/1‑150 (MQ=255)
                                                                                                                                        tataAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGCTCCTCGGCAGCGCTTCCTGTcc        <  1:2599108/150‑1 (MQ=255)
                                                                                                                                        tataAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGCTCCTCGGCAGCGCTTCCTGTcc        <  1:1689100/150‑1 (MQ=255)
                                                                                                                                         ataAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGCTCCTCGGCAGCGCTTCCTGTCCa       >  2:1044702/1‑150 (MQ=255)
                                                                                                                                           aaGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGCTCCTCGGCAGCGCTTCCTGTCCACg     <  2:3831193/150‑1 (MQ=255)
                                                                                                                                           aaGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGCTCCTCGGCAGCGCTTCCTGTCCACg     <  1:370255/150‑1 (MQ=255)
                                                                                                                                              gATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGCTCCTCGGCAGCGCTTCCTGTCCACGAAt  <  1:2679351/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                 
ATATATCCAGAGAATAAAATCTGTCGCAGATAAGGTTGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTCGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGCTCCTCGGCAGCGCTTCCTGTCCACGAAT  >  CP009273/321077‑321368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ATATATCCAGAGAATAAAATCTGTCGCAGATAAGGTTGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTCGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGCTCCTCGGCAGCGCTTCCTGTCCACGAAT  >  CP009273/321077‑321368
                                                                                                                                                  |                                                                                                                                                 
ATATATCCAGAGAATAAAATCTGTCGCAGATAAGGTTGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGT                                                                                                                                                <  SRR19973073.4117694/150‑1 (MQ=60)
ATATATCCAGAGAATAAAATCTGTCGCAGATAAGGTTGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGT                                                                                                                                                <  SRR19973073.4117651/150‑1 (MQ=60)
ATATATCCAGAGAATAAAATCTGTCGCAGATAAGGTTGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGT                                                                                                                                                >  SRR19973073.3937699/1‑150 (MQ=60)
 TATATCCAGAGAATAAAATCTGTCGCAGATAAGGTTGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTC                                                                                                                                               >  SRR19973073.1742302/1‑150 (MQ=60)
   TATCCAGAGAATAAAATCTGTCGCAGATAAGGTTGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCC                                                                                                                                             <  SRR19973073.3706191/150‑1 (MQ=60)
   TATCCAGAGAATAAAATCTGTCGCAGATAAGGTTGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCC                                                                                                                                             <  SRR19973073.3706118/150‑1 (MQ=60)
    ATCCAGAGAATAAAATCTGTCGCAGATAAGNTTGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCC                                                                                                                                            >  SRR19973073.4286414/1‑150 (MQ=60)
    ATCCAGAGAATAAAATCTGTCGCAGATAAGGTTGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCC                                                                                                                                            <  SRR19973073.2019466/150‑1 (MQ=60)
    ATCCAGAGAATAAAATCTGTCGCAGATAAGGTTGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCC                                                                                                                                            >  SRR19973073.4025013/1‑150 (MQ=60)
    ATCCAGAGAATAAAATCTGTCGCAGATAAGGTTGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCC                                                                                                                                            <  SRR19973073.1966443/150‑1 (MQ=60)
          ATAATAAAATCTGTCGCAGATAAGGTTGTATTAATAGTCTGTATCAGGAATGTTCTGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCT                                                                                                                                      <  SRR19973073.1281862/150‑1 (MQ=60)
           GAATAAAATCTGTCGCAGATAAGGTTGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTG                                                                                                                                     >  SRR19973073.3021708/1‑150 (MQ=60)
             ATAAAATCTGTCGCAGATAAGGTTGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTCGGTCCCCCTCTCTGGA                                                                                                                                   <  SRR19973073.4267150/150‑1 (MQ=60)
                       TCGCAGATAAGGTTGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTC                                                                                                                         <  SRR19973073.1362277/150‑1 (MQ=60)
                          CAGATAAGGTTGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTCGGTCCCCCTCTCTGGAACGGTAACTCTCA                                                                                                                      >  SRR19973073.752914/1‑150 (MQ=60)
                          CAGATAAGGTTGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTCGGTCCCCCTCTCTGGAACGGTAACTCTCA                                                                                                                      >  SRR19973073.752902/1‑150 (MQ=60)
                            GATAAGGTTGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAAT                                                                                                                    <  SRR19973073.454106/150‑1 (MQ=60)
                            GATAAGGTTGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAAT                                                                                                                    <  SRR19973073.1405872/150‑1 (MQ=60)
                              TAAGTTTTTATTAATAGTCTGTATCAGTAATGTTCTGGTTAAATATCAGCAATAAGCCCGCATCATGAATACTGTATATGAAGCATGATAGTTACGTCAGTGTTTATATAATGATTAGGTACCGCTCTCTGGATCGGTAACTCTCAATCT                                                                                                                  <  SRR19973073.2194663/150‑1 (MQ=60)
                              TAAGGTTGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCT                                                                                                                  <  SRR19973073.4527269/150‑1 (MQ=60)
                               AAGGTTGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTG                                                                                                                 >  SRR19973073.3307067/1‑150 (MQ=60)
                               AAGGTTGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTG                                                                                                                 >  SRR19973073.3307743/1‑150 (MQ=60)
                                 GGTTGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGAT                                                                                                               >  SRR19973073.1028541/1‑150 (MQ=60)
                                 GGTTGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGAT                                                                                                               >  SRR19973073.564763/1‑150 (MQ=60)
                                 GGTTGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGAT                                                                                                               >  SRR19973073.564758/1‑150 (MQ=60)
                                   TTGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCG                                                                                                             <  SRR19973073.1748244/150‑1 (MQ=60)
                                    TGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGG                                                                                                            >  SRR19973073.3196301/1‑150 (MQ=60)
                                    TGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGG                                                                                                            >  SRR19973073.1483221/1‑150 (MQ=60)
                                    TGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGG                                                                                                            >  SRR19973073.1483224/1‑150 (MQ=60)
                                     GTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGT                                                                                                           >  SRR19973073.1932638/1‑150 (MQ=60)
                                     GTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGT                                                                                                           >  SRR19973073.1932693/1‑150 (MQ=60)
                                       ATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTC                                                                                                         >  SRR19973073.1355336/1‑150 (MQ=60)
                                          AATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTG                                                                                                      >  SRR19973073.3350866/1‑150 (MQ=60)
                                          AATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTG                                                                                                      <  SRR19973073.3904981/150‑1 (MQ=60)
                                           ATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTCGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGC                                                                                                     <  SRR19973073.4569344/150‑1 (MQ=60)
                                            TAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCG                                                                                                    >  SRR19973073.4257154/1‑150 (MQ=60)
                                                CTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAG                                                                                                <  SRR19973073.1015564/150‑1 (MQ=60)
                                                    ATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCA                                                                                            >  SRR19973073.3390973/1‑150 (MQ=60)
                                                       AGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCACAATCAGATCGGTTCCTGCGTTAGTTCACAT                                                                                         >  SRR19973073.1391989/1‑150 (MQ=60)
                                                          AATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCAC                                                                                      <  SRR19973073.278197/150‑1 (MQ=60)
                                                          AATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCAC                                                                                      <  SRR19973073.278456/150‑1 (MQ=60)
                                                            TGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGA                                                                                    >  SRR19973073.433179/1‑150 (MQ=60)
                                                            TGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGA                                                                                    >  SRR19973073.1421456/1‑150 (MQ=60)
                                                              TTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACT                                                                                  >  SRR19973073.1832529/1‑150 (MQ=60)
                                                               TCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTC                                                                                 <  SRR19973073.3310820/150‑1 (MQ=60)
                                                                CGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCA                                                                                <  SRR19973073.2072386/150‑1 (MQ=60)
                                                                 GGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCAT                                                                               <  SRR19973073.2687523/150‑1 (MQ=60)
                                                                 GGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCAT                                                                               <  SRR19973073.2687639/150‑1 (MQ=60)
                                                                 GGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCAT                                                                               <  SRR19973073.2687674/150‑1 (MQ=60)
                                                                   GTTAAATATCATCAAAAAGCCCGCATCATTAATTCTGGATATGAAGAATGAGAGTTACCTCAGTGATTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTATCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTT                                                                             <  SRR19973073.1958411/150‑1 (MQ=60)
                                                                   GTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTT                                                                             <  SRR19973073.4200555/150‑1 (MQ=60)
                                                                    TTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTCGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTT                                                                            <  SRR19973073.3102912/150‑1 (MQ=60)
                                                                     TAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTT                                                                           <  SRR19973073.3835800/150‑1 (MQ=60)
                                                                     TAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTT                                                                           <  SRR19973073.4554439/150‑1 (MQ=60)
                                                                      AAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTT                                                                          <  SRR19973073.709292/150‑1 (MQ=60)
                                                                       AATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTC                                                                         >  SRR19973073.3529050/1‑150 (MQ=60)
                                                                            CAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCT                                                                    <  SRR19973073.720337/150‑1 (MQ=60)
                                                                             AGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTC                                                                   <  SRR19973073.1417587/150‑1 (MQ=60)
                                                                             AGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTC                                                                   <  SRR19973073.2174024/150‑1 (MQ=60)
                                                                              GCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCA                                                                  <  SRR19973073.3401789/150‑1 (MQ=60)
                                                                                AAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACC                                                                >  SRR19973073.2882216/1‑150 (MQ=60)
                                                                                AAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACC                                                                >  SRR19973073.4197512/1‑150 (MQ=60)
                                                                                AAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACC                                                                <  SRR19973073.2541896/150‑1 (MQ=60)
                                                                                 AAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCG                                                               >  SRR19973073.1145819/1‑150 (MQ=60)
                                                                                    AGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCA                                                            >  SRR19973073.4509284/1‑150 (MQ=60)
                                                                                       CCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAATGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCCCCGGCATCC                                                         <  SRR19973073.846854/150‑1 (MQ=60)
                                                                                       CCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCC                                                         <  SRR19973073.846860/150‑1 (MQ=60)
                                                                                         GCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCA                                                       >  SRR19973073.103241/1‑150 (MQ=60)
                                                                                          CATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCAT                                                      >  SRR19973073.1654788/1‑150 (MQ=60)
                                                                                          CATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCAT                                                      >  SRR19973073.1654881/1‑150 (MQ=60)
                                                                                              ATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGC                                                  <  SRR19973073.4227545/150‑1 (MQ=60)
                                                                                                GAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCA                                                <  SRR19973073.3413887/150‑1 (MQ=60)
                                                                                                 AATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCAC                                               <  SRR19973073.3405895/150‑1 (MQ=60)
                                                                                                 AATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCAC                                               >  SRR19973073.1535999/1‑150 (MQ=60)
                                                                                                 AATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCAC                                               >  SRR19973073.1536093/1‑150 (MQ=60)
                                                                                                 AATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCAC                                               >  SRR19973073.1536097/1‑150 (MQ=60)
                                                                                                    ACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAA                                            >  SRR19973073.1536520/1‑150 (MQ=60)
                                                                                                     CTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAA                                           <  SRR19973073.801399/150‑1 (MQ=60)
                                                                                                     CTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAA                                           <  SRR19973073.801251/150‑1 (MQ=60)
                                                                                                     CTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAA                                           >  SRR19973073.629183/1‑150 (MQ=60)
                                                                                                       GGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATA                                         >  SRR19973073.1990295/1‑150 (MQ=60)
                                                                                                       GGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATA                                         >  SRR19973073.3627744/1‑150 (MQ=60)
                                                                                                        GATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATAT                                        <  SRR19973073.2524641/150‑1 (MQ=60)
                                                                                                         ATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATC                                       <  SRR19973073.3399107/150‑1 (MQ=60)
                                                                                                         ATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATC                                       >  SRR19973073.2963494/1‑150 (MQ=60)
                                                                                                          TATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCC                                      >  SRR19973073.3990423/1‑150 (MQ=60)
                                                                                                            TGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCG                                    <  SRR19973073.3112158/150‑1 (MQ=60)
                                                                                                            TGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCACTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCG                                    <  SRR19973073.2811852/150‑1 (MQ=60)
                                                                                                              AAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCC                                  <  SRR19973073.3575777/150‑1 (MQ=60)
                                                                                                              AAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCC                                  <  SRR19973073.3575804/150‑1 (MQ=60)
                                                                                                                GCATGAGAGTTACCTCAGTGTTTATATAAGGATTATGTCACCCTCTCTGGAAAGGTAACTCTCAATCTGTTCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACAGGCATCCCATTTGCCACAAAATATCCCGCCGT                                <  SRR19973073.3666325/150‑1 (MQ=60)
                                                                                                                 CATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTG                               >  SRR19973073.2907697/1‑150 (MQ=60)
                                                                                                                 CATGAGAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAAGCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTG                               >  SRR19973073.2907674/1‑150 (MQ=60)
                                                                                                                      GAGTTACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGCTCCT                          >  SRR19973073.4549506/1‑150 (MQ=60)
                                                                                                                          TACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGCTCCTCGGC                      >  SRR19973073.2990724/1‑150 (MQ=60)
                                                                                                                          TACCTCAGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGCTCCTCGGC                      >  SRR19973073.2990730/1‑150 (MQ=60)
                                                                                                                                AGTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGCTCCTCGGCAGCGCT                <  SRR19973073.4539132/150‑1 (MQ=60)
                                                                                                                                 GTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGCTCCTCGGCAGCGCTT               >  SRR19973073.595627/1‑150 (MQ=60)
                                                                                                                                 GTGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGCTCCTCGGCAGCGCTT               >  SRR19973073.2873105/1‑150 (MQ=60)
                                                                                                                                  TGTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGCTCCTCGGCAGCGCTTC              >  SRR19973073.3696111/1‑150 (MQ=60)
                                                                                                                                   GTTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGCTCCTCGGCAGCGCTTCC             <  SRR19973073.1519220/150‑1 (MQ=60)
                                                                                                                                    TTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGCTCCTCGGCAGCGCTTCCT            <  SRR19973073.4343795/150‑1 (MQ=60)
                                                                                                                                    TTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGCTCCTCGGCAGCGCTTCCT            <  SRR19973073.4313396/150‑1 (MQ=60)
                                                                                                                                    TTTATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGCTCCTCGGCAGCGCTTCCT            <  SRR19973073.2630721/150‑1 (MQ=60)
                                                                                                                                       ATATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGCTCCTCGGCAGCGCTTCCTGTC         >  SRR19973073.1440093/1‑150 (MQ=60)
                                                                                                                                        TATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGCTCCTCGGCAGCGCTTCCTGTCC        <  SRR19973073.2604457/150‑1 (MQ=60)
                                                                                                                                        TATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGCTCCTCGGCAGCGCTTCCTGTCC        <  SRR19973073.1692606/150‑1 (MQ=60)
                                                                                                                                        TATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGCTCCTCGGCAGCGCTTCCTGTCC        <  SRR19973073.1692619/150‑1 (MQ=60)
                                                                                                                                        TATAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGCTCCTCGGCAGCGCTTCCTGTCC        <  SRR19973073.2604445/150‑1 (MQ=60)
                                                                                                                                          TAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGCTCCTCGGCAGCGCTTCCTGTCCAC      <  SRR19973073.3698775/150‑1 (MQ=60)
                                                                                                                                          TAAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGCTCCTCGGCAGCGCTTCCTGTCCAC      <  SRR19973073.3698747/150‑1 (MQ=60)
                                                                                                                                           AAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGCTCCTCGGCAGCGCTTCCTGTCCACG     <  SRR19973073.370986/150‑1 (MQ=60)
                                                                                                                                           AAGGATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGCTCCTCGGCAGCGCTTCCTGTCCACG     <  SRR19973073.528239/150‑1 (MQ=60)
                                                                                                                                              GATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGCTCCTCGGCAGCGCTTCCTGTCCACGAAT  <  SRR19973073.1589905/150‑1 (MQ=60)
                                                                                                                                              GATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGCTCCTCGGCAGCGCTTCCTGTCCACGAAT  <  SRR19973073.2684829/150‑1 (MQ=60)
                                                                                                                                              GATTAGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGCTCCTCGGCAGCGCTTCCTGTCCACGAAT  <  SRR19973073.1134563/150‑1 (MQ=60)
                                                                                                                                                  |                                                                                                                                                 
ATATATCCAGAGAATAAAATCTGTCGCAGATAAGGTTGTATTAATAGTCTGTATCAGGAATGTTCGGGTTAAATATCAGCAAAAAGCCCGCATCATGAATACTGGATATGAAGCATGAGAGTTACCTCAGTGTTTATATAAGGATTCGGTCCCCCTCTCTGGAACGGTAACTCTCAATCTGATCGGTTCCTGCGTTAGTTCACATCACGACTCATTTTTTCGCTCTCACCGGCATCCCATTTGCCACAAAATATCCCGCCGTGCTCCTCGGCAGCGCTTCCTGTCCACGAAT  >  CP009273/321077‑321368

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑