Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,172,212 C→A 13.5% S46S (TCC→TCA)  lolD → outer membrane‑specific lipoprotein transporter subunit

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,172,2120CA13.5% 738.3 / 64.4 220S46S (TCC→TCA) lolDouter membrane‑specific lipoprotein transporter subunit
Reads supporting (aligned to +/- strand):  ref base C (102/88);  new base A (12/18);  total (114/106)
Fisher's exact test for biased strand distribution p-value = 1.75e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.81e-01

Only 100 of 221 total aligned reads displayed.

GGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTC  >  CP009273/1172064‑1172360
                                                                                                                                                    |                                                                                                                                                    
 gCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCgg                                                                                                                                                    >  1:1386793/1‑150 (MQ=255)
 gCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCgg                                                                                                                                                    <  2:1545204/150‑1 (MQ=255)
  cTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGt                                                                                                                                                   <  1:2697386/150‑1 (MQ=255)
    ttACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAATCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCAGGTaa                                                                                                                                                 <  1:2702793/150‑1 (MQ=255)
     tACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTaaa                                                                                                                                                >  2:4399544/1‑150 (MQ=255)
       cGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAg                                                                                                                                              >  2:4546238/1‑150 (MQ=255)
       cGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAg                                                                                                                                              >  2:869452/1‑150 (MQ=255)
        gTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGt                                                                                                                                             >  1:202320/1‑150 (MQ=255)
          tATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTAc                                                                                                                                           >  1:1821874/1‑150 (MQ=255)
           aTGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTAcc                                                                                                                                          <  2:3148531/150‑1 (MQ=255)
           aTGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTAcc                                                                                                                                          <  2:3148571/150‑1 (MQ=255)
            tGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCt                                                                                                                                         >  1:527825/1‑150 (MQ=255)
             gAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCtt                                                                                                                                        <  2:1053451/150‑1 (MQ=255)
             gAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCtt                                                                                                                                        <  2:908488/150‑1 (MQ=255)
                tAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTtgct                                                                                                                                     <  1:2973940/150‑1 (MQ=255)
                 aaGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTtgctg                                                                                                                                    >  1:1310132/1‑150 (MQ=255)
                  aGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAACCCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCAGGTAAAAGTACCTtgctgc                                                                                                                                   <  2:3961903/150‑1 (MQ=255)
                  aGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTtgctgc                                                                                                                                   >  1:3777170/1‑150 (MQ=255)
                  aGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTtgctgc                                                                                                                                   >  1:4583370/1‑150 (MQ=255)
                     tCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCAcc                                                                                                                                >  2:3453682/1‑150 (MQ=255)
                      ccTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCAGGTAAAAGTACCTTGCTGCACct                                                                                                                               >  1:1833868/1‑150 (MQ=255)
                        tGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACctgc                                                                                                                             >  1:3627813/1‑150 (MQ=255)
                           tGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTgg                                                                                                                          >  1:160655/1‑150 (MQ=255)
                           tGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTgg                                                                                                                          >  1:160663/1‑150 (MQ=255)
                           tGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTgg                                                                                                                          >  2:1561133/1‑150 (MQ=255)
                             cAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTgggc                                                                                                                        >  2:892598/1‑150 (MQ=255)
                                  cGACAACCTGTGCAAACGCTATCAGGAAGTCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTAGGTAGCTCTGTTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCt                                                                                                                   <  2:2449072/150‑1 (MQ=255)
                                   gACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTg                                                                                                                  <  2:1783124/150‑1 (MQ=255)
                                     cAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCGCTGGTTCAGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGa                                                                                                                >  1:1787764/1‑150 (MQ=255)
                                      aaCCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGAt                                                                                                               <  2:2508059/150‑1 (MQ=255)
                                        ccTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATAc                                                                                                             >  1:3353167/1‑150 (MQ=255)
                                         cTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCAGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACg                                                                                                            <  2:1107919/150‑1 (MQ=255)
                                           gtgCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCAGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGcc                                                                                                          >  2:1767419/1‑150 (MQ=255)
                                              cAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAAc                                                                                                       >  1:107035/1‑150 (MQ=255)
                                              cAAACGCTATCAGGAAGGCAGTGTGCAAACCGAGGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTTAAATGATGGCGATCGTCGGTAGCACTGGTTCAGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAAc                                                                                                       <  2:606020/150‑1 (MQ=255)
                                               aaaCGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAAcc                                                                                                      <  1:3417916/150‑1 (MQ=255)
                                               aaaCGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAAcc                                                                                                      >  2:3777036/1‑150 (MQ=255)
                                                aaCGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAAccc                                                                                                     >  1:109764/1‑149 (MQ=255)
                                                aaCGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCt                                                                                                     >  1:1718310/1‑150 (MQ=255)
                                                 aCGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTc                                                                                                    >  1:1243672/1‑150 (MQ=255)
                                                  cGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTcc                                                                                                   >  2:2028540/1‑150 (MQ=255)
                                                  cGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTcc                                                                                                   >  1:1023482/1‑150 (MQ=255)
                                                  cGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTcc                                                                                                   <  2:1343153/150‑1 (MQ=255)
                                                    cTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCgg                                                                                                 <  1:3733467/150‑1 (MQ=255)
                                                    cTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCAGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCgg                                                                                                 >  2:132797/1‑150 (MQ=255)
                                                     tATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGc                                                                                                >  2:948193/1‑150 (MQ=255)
                                                       tCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGa                                                                                              >  1:4479252/1‑150 (MQ=255)
                                                        cAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGAt                                                                                             <  1:2198585/150‑1 (MQ=255)
                                                         aGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGAtg                                                                                            >  1:2575373/1‑150 (MQ=255)
                                                         aGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGAtg                                                                                            >  1:4286403/1‑150 (MQ=255)
                                                           gAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGAtgtg                                                                                          >  2:2269682/1‑150 (MQ=255)
                                                                 aGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGAttttt                                                                                    >  1:4070645/1‑150 (MQ=255)
                                                                  gtgtGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTa                                                                                   >  1:393669/1‑150 (MQ=255)
                                                                     tgCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATg                                                                                <  1:1865034/150‑1 (MQ=255)
                                                                        aaaCCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTc                                                                             <  2:413182/150‑1 (MQ=255)
                                                                           ccGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGc                                                                          <  2:4037418/150‑1 (MQ=255)
                                                                            cGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGcc                                                                         >  2:3988489/1‑150 (MQ=255)
                                                                            cGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGcc                                                                         >  2:1666927/1‑150 (MQ=255)
                                                                             gATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCAGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCa                                                                        <  2:796565/150‑1 (MQ=255)
                                                                                  gctgcACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAg                                                                   <  2:1852719/150‑1 (MQ=255)
                                                                                   ctgcACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGc                                                                  <  2:2000073/150‑1 (MQ=255)
                                                                                   ctgcACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCAGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGc                                                                  >  1:3648028/1‑150 (MQ=255)
                                                                                   ctgcACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCAGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGc                                                                  <  2:3312968/150‑1 (MQ=255)
                                                                                    tgcACAATGTCAGTTTCAGCGTCAGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCAGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCa                                                                 >  1:3454171/1‑150 (MQ=255)
                                                                                      cacaATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCaaa                                                               >  2:1482570/1‑150 (MQ=255)
                                                                                        caATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACt                                                             <  1:882130/150‑1 (MQ=255)
                                                                                            gTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTgtcg                                                         >  1:1961033/1‑150 (MQ=255)
                                                                                             tCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTgtcgt                                                        >  1:158669/1‑150 (MQ=255)
                                                                                              cAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTgtcgtc                                                       <  2:816734/150‑1 (MQ=255)
                                                                                               aGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGtcgtcg                                                      >  1:1933642/1‑150 (MQ=255)
                                                                                               aGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCAGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGtcgtcg                                                      <  1:2890690/150‑1 (MQ=255)
                                                                                                 tttCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGc                                                    >  2:1621636/1‑150 (MQ=255)
                                                                                                    cAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTATCGTCGGCAGc                                                 >  1:768061/1‑150 (MQ=255)
                                                                                                       cGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCACTGAGCAAACTGTCGTCGGCAGCGaa                                              >  2:2887954/1‑150 (MQ=255)
                                                                                                       cGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGaa                                              >  2:4085057/1‑150 (MQ=255)
                                                                                                        gTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGaaa                                             >  2:2685099/1‑150 (MQ=255)
                                                                                                        gTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGaaa                                             <  2:2839203/150‑1 (MQ=255)
                                                                                                        gTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGaaa                                             >  2:1824488/1‑150 (MQ=255)
                                                                                                           ggCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCt                                          <  2:2999742/150‑1 (MQ=255)
                                                                                                            gCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTg                                         <  2:3299214/150‑1 (MQ=255)
                                                                                                              gAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGaa                                       <  1:1760703/150‑1 (MQ=255)
                                                                                                              gAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGaa                                       >  1:4247764/1‑150 (MQ=255)
                                                                                                              gAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGaa                                       >  1:546777/1‑150 (MQ=255)
                                                                                                              gAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGaa                                       >  1:863689/1‑150 (MQ=255)
                                                                                                               aaGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAAc                                      >  2:3015385/1‑150 (MQ=255)
                                                                                                               aaGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAAc                                      >  2:3957851/1‑150 (MQ=255)
                                                                                                                aGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCTGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACt                                     <  2:3785478/150‑1 (MQ=255)
                                                                                                                aGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACt                                     <  1:1453106/150‑1 (MQ=255)
                                                                                                                aGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACt                                     >  1:3288518/1‑150 (MQ=255)
                                                                                                                 ggTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTg                                    >  2:3640992/1‑150 (MQ=255)
                                                                                                                 ggTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTg                                    >  2:2151659/1‑150 (MQ=255)
                                                                                                                       atgatgGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCacc                              >  2:252707/1‑148 (MQ=255)
                                                                                                                        tgatgGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAAcc                             <  1:4625342/150‑1 (MQ=255)
                                                                                                                                     cgGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCtt                >  2:4075837/1‑150 (MQ=255)
                                                                                                                                      ggTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCttt               >  2:2496830/1‑150 (MQ=255)
                                                                                                                                            tctGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTttatttat         <  2:410190/150‑1 (MQ=255)
                                                                                                                                            tctGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTttatttat         >  1:2713969/1‑150 (MQ=255)
                                                                                                                                             ctGGTTCAGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATc        >  2:3769076/1‑150 (MQ=255)
                                                                                                                                                  tCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGttt   <  2:4253964/150‑1 (MQ=255)
                                                                                                                                                  tCCGGTAAAAGTACCTGGCTGCACCTGCTGGGCGGGCTGGAGACGCCACCCTCCGGCGATGTGATTTTTACTGGTCCGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGttt   <  2:3888807/150‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GGCTTTACGTTATGAATAAGATCCTGTTGCAATGCGACAACCTGTGCAAACGCTATCAGGAAGGCAGTGTGCAAACCGATGTGCTGCACAATGTCAGTTTCAGCGTCGGCGAAGGTGAAATGATGGCGATCGTCGGTAGCTCTGGTTCCGGTAAAAGTACCTTGCTGCACCTGCTGGGCGGGCTGGATACGCCAACCTCCGGCGATGTGATTTTTAATGGTCAGCCAATGAGCAAACTGTCGTCGGCAGCGAAAGCTGAACTGCGCAACCAGAAGCTGGGCTTTATTTATCAGTTTC  >  CP009273/1172064‑1172360

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A