Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,576,844 G→A 54.1% intergenic (‑63/+339) ydeN ← / ← ydeO putative Ser‑type periplasmic non‑aryl sulfatase/UV‑inducible global regulator, EvgA‑, GadE‑dependent

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,576,8440GA54.1% 51.6 / inf 270intergenic (‑63/+339)ydeN/ydeOputative Ser‑type periplasmic non‑aryl sulfatase/UV‑inducible global regulator, EvgA‑, GadE‑dependent
Reads supporting (aligned to +/- strand):  ref base G (61/63);  new base A (77/69);  total (138/132)
Fisher's exact test for biased strand distribution p-value = 6.25e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.95e-01

Only 100 of 276 total aligned reads displayed.

CGCATGAGCAGCAAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTATTGATATTTAATAAATTATTGCA  >  CP009273/1576695‑1576992
                                                                                                                                                     |                                                                                                                                                    
cGCATGAGCAGCAAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATg                                                                                                                                                      <  1:4227272/150‑1 (MQ=255)
   aTGAGCAGCAAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATaa                                                                                                                                                   <  2:2941603/150‑1 (MQ=255)
    tGAGCAGCAAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAg                                                                                                                                                  >  1:3734021/1‑150 (MQ=255)
     gAGCAGCAAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGt                                                                                                                                                 >  2:3882819/1‑150 (MQ=255)
      agcagcAAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTg                                                                                                                                                >  1:743130/1‑150 (MQ=255)
      agcagcAAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTg                                                                                                                                                <  1:4477332/150‑1 (MQ=255)
        cagcaAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGaa                                                                                                                                              <  2:3949985/150‑1 (MQ=255)
         agcaAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAg                                                                                                                                             >  1:4608831/1‑150 (MQ=255)
         agcaAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAg                                                                                                                                             <  2:2052342/150‑1 (MQ=255)
          gcaAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGt                                                                                                                                            >  1:2314341/1‑150 (MQ=255)
           caAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGtt                                                                                                                                           <  1:548109/150‑1 (MQ=255)
           caAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGtt                                                                                                                                           <  1:2961286/150‑1 (MQ=255)
              aTGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGt                                                                                                                                        <  2:2539483/150‑1 (MQ=255)
               tGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTc                                                                                                                                       <  1:895038/150‑1 (MQ=255)
                    ccATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAACTAAGTGGATATAAGTGAAGTTAGTCACATa                                                                                                                                  >  2:307376/1‑150 (MQ=255)
                      aTACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATaaa                                                                                                                                >  2:835651/1‑150 (MQ=255)
                      aTACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATaaa                                                                                                                                <  1:393940/150‑1 (MQ=255)
                           aGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGAt                                                                                                                           >  2:3786780/1‑150 (MQ=255)
                           aGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGAt                                                                                                                           <  1:3098047/150‑1 (MQ=255)
                               gCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCa                                                                                                                       <  1:1171207/150‑1 (MQ=255)
                               gCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCa                                                                                                                       <  1:3549874/150‑1 (MQ=255)
                               gCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCa                                                                                                                       >  1:2453301/1‑150 (MQ=255)
                               gCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCa                                                                                                                       <  2:1237954/150‑1 (MQ=255)
                                  aGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGAt                                                                                                                    >  2:147557/1‑150 (MQ=255)
                                   gTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGAtt                                                                                                                   <  1:3168230/150‑1 (MQ=255)
                                    tATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGAttt                                                                                                                  <  2:611497/150‑1 (MQ=255)
                                     aTCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTa                                                                                                                 >  2:1357730/1‑150 (MQ=255)
                                       cAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGc                                                                                                               >  1:2111878/1‑150 (MQ=255)
                                       cAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGc                                                                                                               <  1:2735675/150‑1 (MQ=255)
                                        aaaGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCt                                                                                                              >  2:1225936/1‑150 (MQ=255)
                                        aaaGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCt                                                                                                              <  1:1825428/150‑1 (MQ=255)
                                           gATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTaaa                                                                                                           <  2:1051326/150‑1 (MQ=255)
                                             tatCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTaaaaa                                                                                                         <  1:1962434/150‑1 (MQ=255)
                                              atCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTaaaaaa                                                                                                        <  2:4144811/150‑1 (MQ=255)
                                                 gAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAgg                                                                                                     >  2:2570942/1‑150 (MQ=255)
                                                      aCTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGaaaa                                                                                                <  1:1365771/150‑1 (MQ=255)
                                                      aCTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGaaaa                                                                                                <  1:3251661/150‑1 (MQ=255)
                                                       cTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGaaaaa                                                                                               >  1:1795969/1‑150 (MQ=255)
                                                        ttACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGaaaaaa                                                                                              >  2:3699110/1‑150 (MQ=255)
                                                         tACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAAc                                                                                             <  1:1050946/150‑1 (MQ=255)
                                                           cGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGACAAAACAg                                                                                           >  2:3770941/1‑150 (MQ=255)
                                                              cacTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTcc                                                                                        <  1:3899378/150‑1 (MQ=255)
                                                               acTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCa                                                                                       <  2:750978/150‑1 (MQ=255)
                                                               acTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCa                                                                                       <  2:1275073/150‑1 (MQ=255)
                                                                cTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCAt                                                                                      <  2:973016/150‑1 (MQ=255)
                                                                    tCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAg                                                                                  <  1:2070791/150‑1 (MQ=255)
                                                                             cAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACaa                                                                         >  2:433588/1‑149 (MQ=255)
                                                                             cAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACAt                                                                         >  2:3618313/1‑150 (MQ=255)
                                                                             cAGACTTAATAAATGTTCCTCTTCTTATTGTAATTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACAt                                                                         <  1:2629849/150‑1 (MQ=255)
                                                                              aGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACAtt                                                                        >  2:220802/1‑150 (MQ=255)
                                                                               gACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTa                                                                       <  2:2114320/150‑1 (MQ=255)
                                                                               gACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTa                                                                       >  1:1419869/1‑150 (MQ=255)
                                                                                aCTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTAc                                                                      >  1:2347550/1‑150 (MQ=255)
                                                                                aCTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTAc                                                                      >  1:4185249/1‑150 (MQ=255)
                                                                                 cTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACt                                                                     >  1:616237/1‑150 (MQ=255)
                                                                                   tCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACttt                                                                   <  2:2758814/150‑1 (MQ=255)
                                                                                   tCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACttt                                                                   <  2:2758798/150‑1 (MQ=255)
                                                                                      tAAATGGTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTg                                                                <  1:2557784/150‑1 (MQ=255)
                                                                                        aaTGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGtt                                                              >  1:2214119/1‑150 (MQ=255)
                                                                                        aaTGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGtt                                                              >  1:2953577/1‑150 (MQ=255)
                                                                                         aTGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTc                                                             >  2:3212432/1‑150 (MQ=255)
                                                                                         aTGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTc                                                             >  1:425907/1‑150 (MQ=255)
                                                                                           gTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTa                                                           >  2:4411568/1‑150 (MQ=255)
                                                                                           gTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTa                                                           <  1:3230244/150‑1 (MQ=255)
                                                                                            ttCCTCTTCTTATTTTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTACATAAAGCGTTGACATTACTTTCTGTTCTAt                                                          <  1:1079746/150‑1 (MQ=255)
                                                                                             tCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTAtt                                                         <  2:45597/150‑1 (MQ=255)
                                                                                               ctctTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTaa                                                       >  2:1388838/1‑150 (MQ=255)
                                                                                                  ttcttATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTGCTATTAAGTa                                                    >  2:4220664/1‑150 (MQ=255)
                                                                                                         tGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTtctc                                             >  2:685016/1‑150 (MQ=255)
                                                                                                            aCTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGcc                                          >  2:1578359/1‑150 (MQ=255)
                                                                                                            aCTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGcc                                          >  2:4579892/1‑150 (MQ=255)
                                                                                                             cTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCg                                         <  2:3026542/150‑1 (MQ=255)
                                                                                                                 aTTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATaa                                     >  2:2405142/1‑150 (MQ=255)
                                                                                                                    ccAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAaaca                                  <  1:1869216/150‑1 (MQ=255)
                                                                                                                     cAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAaacaa                                 >  1:1493553/1‑150 (MQ=255)
                                                                                                                      aaaGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAaacaac                                >  1:2674890/1‑150 (MQ=255)
                                                                                                                       aaGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACt                               >  1:1707409/1‑150 (MQ=255)
                                                                                                                         gTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTaa                             <  2:349201/150‑1 (MQ=255)
                                                                                                                          tAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAAt                            >  1:366067/1‑150 (MQ=255)
                                                                                                                           aaTTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAAtt                           >  1:1806518/1‑150 (MQ=255)
                                                                                                                            aTTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAAgtt                          <  2:4215142/150‑1 (MQ=255)
                                                                                                                            aTTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTACTCGCCGATAAACAACTAAttt                          >  2:1834705/1‑150 (MQ=255)
                                                                                                                              ttttCGAAGAGTAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTAt                        <  1:3639415/150‑1 (MQ=255)
                                                                                                                              tttACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTAt                        <  1:232614/150‑1 (MQ=255)
                                                                                                                               ttACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTAtt                       >  2:3242946/1‑150 (MQ=255)
                                                                                                                               ttACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTAtt                       <  2:1501089/150‑1 (MQ=255)
                                                                                                                               ttACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTAtt                       >  2:1590533/1‑150 (MQ=255)
                                                                                                                                  cGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTATTGat                    >  1:3965986/1‑150 (MQ=255)
                                                                                                                                   gAAGAGAAATAAGTTGATGTAAGTGAAGTTAGTCACAAAAAGAGATAGAAGATTTAGATAAAAAAATTTAAAAAACATTCCATAAATCGTTGACATTACGTTCTGTTCTATTAATTAATTTCTCGCCGATAAACAACTAAAATATTGata                   >  2:3590928/1‑150 (MQ=255)
                                                                                                                                     agagAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTATTGATAtt                 >  2:3756237/1‑150 (MQ=255)
                                                                                                                                     agagAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTATTGATAtt                 <  1:3377876/150‑1 (MQ=255)
                                                                                                                                      gagaAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTATTGATAttt                <  2:4266461/150‑1 (MQ=255)
                                                                                                                                      gagaAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTATTGATAttt                >  1:777362/1‑150 (MQ=255)
                                                                                                                                       agaAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTATTGATATTta               >  2:1968259/1‑150 (MQ=255)
                                                                                                                                       agaAATAAGTGCAAATAAGTGAAGTTAGTCACATAAAGAGATAGCATATTTAGCTATAAAAAGGTAAAAAACAGTCCATAAAGAGTTGAAATAACTTTCTGTTCTATTAATTAATTTCTCGCCGATAAACAACTAATTTATTGATATTta               >  2:2612834/1‑150 (MQ=255)
                                                                                                                                        gaAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTATTGATATTtaa              >  2:924464/1‑150 (MQ=255)
                                                                                                                                           aTAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTATTGATATTtaataa           >  2:2435182/1‑150 (MQ=255)
                                                                                                                                            tAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTATTGATATTTAATaaa          >  2:1328471/1‑150 (MQ=255)
                                                                                                                                            tAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGAAGATTTAGCTAAAAAAAGGGAAAAAACAGTACATAAAGCGTTGACATTACGTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTATTGATATTTAATaaa          >  1:247542/1‑150 (MQ=255)
                                                                                                                                              aGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTATTGATATTTAATAAatt        >  1:2816366/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                    
CGCATGAGCAGCAAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTATTGATATTTAATAAATTATTGCA  >  CP009273/1576695‑1576992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGCATGAGCAGCAAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTATTGATATTTAATAAATTATTG  >  CP009273/1576695‑1576990
                                                                                                                                                     |                                                                                                                                                  
CGCCTGAGCAGCACATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATA                                                                                                                                                    <  SRR19973073.810010/150‑1 (MQ=60)
CGCATGAGCAGCAAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATG                                                                                                                                                    <  SRR19973073.4235650/150‑1 (MQ=60)
CGCATGAGCAGCAAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATA                                                                                                                                                    >  SRR19973073.581421/1‑150 (MQ=60)
CGCATGAGCAGCAAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACGTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATA                                                                                                                                                    <  SRR19973073.809788/150‑1 (MQ=60)
 GCATGAGCAGCAAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGT                                                                                                                                                   >  SRR19973073.1107440/1‑150 (MQ=60)
  CATGAGCAGCAAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTA                                                                                                                                                  >  SRR19973073.3926489/1‑150 (MQ=60)
    TGAGCAGCAAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAG                                                                                                                                                >  SRR19973073.3741520/1‑150 (MQ=60)
      AGCAGCAAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTG                                                                                                                                              <  SRR19973073.1862093/150‑1 (MQ=60)
      AGCAGCAAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTG                                                                                                                                              >  SRR19973073.744639/1‑150 (MQ=60)
      AGCAGCAAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTG                                                                                                                                              <  SRR19973073.4486331/150‑1 (MQ=60)
       GCAGCAAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGA                                                                                                                                             <  SRR19973073.3597258/150‑1 (MQ=60)
       GCAGCAAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGA                                                                                                                                             <  SRR19973073.3597106/150‑1 (MQ=60)
        CAGCAAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAA                                                                                                                                            <  SRR19973073.4492412/150‑1 (MQ=60)
         AGCAAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAG                                                                                                                                           >  SRR19973073.571758/1‑150 (MQ=60)
         AGCAAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAG                                                                                                                                           >  SRR19973073.4618020/1‑150 (MQ=60)
         AGCAAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAG                                                                                                                                           >  SRR19973073.4618029/1‑150 (MQ=60)
          GCAAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGT                                                                                                                                          >  SRR19973073.2319054/1‑150 (MQ=60)
          GCAAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGT                                                                                                                                          >  SRR19973073.2318988/1‑150 (MQ=60)
          GCAAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGT                                                                                                                                          >  SRR19973073.2318874/1‑150 (MQ=60)
           CAAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTT                                                                                                                                         <  SRR19973073.549289/150‑1 (MQ=60)
           CAAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTT                                                                                                                                         <  SRR19973073.509252/150‑1 (MQ=60)
           CAAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTT                                                                                                                                         <  SRR19973073.509115/150‑1 (MQ=60)
           CAAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTT                                                                                                                                         >  SRR19973073.4374936/1‑150 (MQ=60)
           CAAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTT                                                                                                                                         <  SRR19973073.2967319/150‑1 (MQ=60)
               TGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTC                                                                                                                                     <  SRR19973073.896887/150‑1 (MQ=60)
                 CAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCAC                                                                                                                                   <  SRR19973073.1573184/150‑1 (MQ=60)
                  ATCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACA                                                                                                                                  <  SRR19973073.3300757/150‑1 (MQ=60)
                  AGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACA                                                                                                                                  >  SRR19973073.2662423/1‑150 (MQ=60)
                  AGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACA                                                                                                                                  >  SRR19973073.79219/1‑150 (MQ=60)
                   GCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACAT                                                                                                                                 <  SRR19973073.3070243/150‑1 (MQ=60)
                   GCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACAT                                                                                                                                 >  SRR19973073.1718473/1‑150 (MQ=60)
                   GCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACAT                                                                                                                                 <  SRR19973073.749122/150‑1 (MQ=60)
                      ATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAA                                                                                                                              <  SRR19973073.394724/150‑1 (MQ=60)
                      ATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAA                                                                                                                              >  SRR19973073.1493724/1‑150 (MQ=60)
                           AGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGAT                                                                                                                         <  SRR19973073.3104281/150‑1 (MQ=60)
                           AGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGAT                                                                                                                         <  SRR19973073.3104273/150‑1 (MQ=60)
                            GATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATA                                                                                                                        <  SRR19973073.2077614/150‑1 (MQ=60)
                               GCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCA                                                                                                                     <  SRR19973073.3556986/150‑1 (MQ=60)
                               GCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCA                                                                                                                     <  SRR19973073.1173599/150‑1 (MQ=60)
                               GCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCA                                                                                                                     >  SRR19973073.2458386/1‑150 (MQ=60)
                               GCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCA                                                                                                                     >  SRR19973073.1809022/1‑150 (MQ=60)
                               GCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCA                                                                                                                     >  SRR19973073.2458453/1‑150 (MQ=60)
                               GCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCA                                                                                                                     >  SRR19973073.2746134/1‑150 (MQ=60)
                                   GTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATT                                                                                                                 <  SRR19973073.4091687/150‑1 (MQ=60)
                                   GTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATT                                                                                                                 <  SRR19973073.3174617/150‑1 (MQ=60)
                                       CAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGC                                                                                                             <  SRR19973073.2741263/150‑1 (MQ=60)
                                       CAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGC                                                                                                             <  SRR19973073.2741257/150‑1 (MQ=60)
                                       CAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGC                                                                                                             <  SRR19973073.2741213/150‑1 (MQ=60)
                                       CAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGC                                                                                                             >  SRR19973073.2116449/1‑150 (MQ=60)
                                       CAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGC                                                                                                             >  SRR19973073.2116295/1‑150 (MQ=60)
                                       CAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGC                                                                                                             <  SRR19973073.4029027/150‑1 (MQ=60)
                                        AAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCT                                                                                                            <  SRR19973073.4200326/150‑1 (MQ=60)
                                        AAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCT                                                                                                            <  SRR19973073.1829199/150‑1 (MQ=60)
                                         AAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTA                                                                                                           >  SRR19973073.3812578/1‑150 (MQ=60)
                                         AAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTA                                                                                                           >  SRR19973073.3946097/1‑150 (MQ=60)
                                            ATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAA                                                                                                        >  SRR19973073.3447329/1‑150 (MQ=60)
                                            ATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAA                                                                                                        >  SRR19973073.4207676/1‑150 (MQ=60)
                                             TATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAA                                                                                                       <  SRR19973073.1966481/150‑1 (MQ=60)
                                                      ACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAA                                                                                              <  SRR19973073.1368668/150‑1 (MQ=60)
                                                      ACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAA                                                                                              <  SRR19973073.1368667/150‑1 (MQ=60)
                                                      ACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAA                                                                                              <  SRR19973073.330096/150‑1 (MQ=60)
                                                      ACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAA                                                                                              <  SRR19973073.3258237/150‑1 (MQ=60)
                                                       CTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAA                                                                                             >  SRR19973073.1799678/1‑150 (MQ=60)
                                                       CTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAA                                                                                             >  SRR19973073.1799253/1‑150 (MQ=60)
                                                         TACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAAC                                                                                           <  SRR19973073.2812051/150‑1 (MQ=60)
                                                         TACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAAC                                                                                           <  SRR19973073.1308700/150‑1 (MQ=60)
                                                         TACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAAC                                                                                           <  SRR19973073.1053132/150‑1 (MQ=60)
                                                          ACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACA                                                                                          <  SRR19973073.3687454/150‑1 (MQ=60)
                                                           CGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAG                                                                                         >  SRR19973073.3673215/1‑150 (MQ=60)
                                                             ACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGAAATTATACGAAGAGAAATAACGAGATATAAGTGAAGTTAGTCACATAAAGAGATCGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTC                                                                                       >  SRR19973073.646095/1‑150 (MQ=60)
                                                              CACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCC                                                                                      <  SRR19973073.3907123/150‑1 (MQ=60)
                                                                 TTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATA                                                                                   <  SRR19973073.1074651/150‑1 (MQ=60)
                                                                 TTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATA                                                                                   >  SRR19973073.2073991/1‑150 (MQ=60)
                                                                    TCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAG                                                                                <  SRR19973073.2075112/150‑1 (MQ=60)
                                                                    TCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAG                                                                                <  SRR19973073.2075100/150‑1 (MQ=60)
                                                                     CTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGC                                                                               <  SRR19973073.2658137/150‑1 (MQ=60)
                                                                        TAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTT                                                                            >  SRR19973073.1940401/1‑150 (MQ=60)
                                                                        TAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTT                                                                            >  SRR19973073.1940323/1‑150 (MQ=60)
                                                                         AATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTATGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTG                                                                           >  SRR19973073.2479662/1‑150 (MQ=60)
                                                                         AATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTG                                                                           >  SRR19973073.3240575/1‑150 (MQ=60)
                                                                             CAGACTTAATAAATGTTCCTCTTCTTATTGTAATTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACAT                                                                       <  SRR19973073.2635250/150‑1 (MQ=60)
                                                                              AGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATT                                                                      <  SRR19973073.95144/150‑1 (MQ=60)
                                                                               GACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTA                                                                     >  SRR19973073.1422822/1‑150 (MQ=60)
                                                                               GACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTA                                                                     >  SRR19973073.1422859/1‑150 (MQ=60)
                                                                                ACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTAC                                                                    <  SRR19973073.3117667/150‑1 (MQ=60)
                                                                                ACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTAC                                                                    >  SRR19973073.4193567/1‑150 (MQ=60)
                                                                                ACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTAC                                                                    >  SRR19973073.2352351/1‑150 (MQ=60)
                                                                                 CTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACT                                                                   >  SRR19973073.617553/1‑150 (MQ=60)
                                                                                  TTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTT                                                                  <  SRR19973073.1847624/150‑1 (MQ=60)
                                                                                   TCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTT                                                                 >  SRR19973073.2341191/1‑150 (MQ=60)
                                                                                      TAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTG                                                              <  SRR19973073.241146/150‑1 (MQ=60)
                                                                                      TAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTG                                                              >  SRR19973073.1136887/1‑150 (MQ=60)
                                                                                      TAAATGGTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTG                                                              <  SRR19973073.2563058/150‑1 (MQ=60)
                                                                                       AAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGT                                                             <  SRR19973073.2590590/150‑1 (MQ=60)
                                                                                        AATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTAATTTCTGTT                                                            >  SRR19973073.3824745/1‑150 (MQ=60)
                                                                                        AATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTT                                                            >  SRR19973073.2218710/1‑150 (MQ=60)
                                                                                        AATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTT                                                            >  SRR19973073.2959599/1‑150 (MQ=60)
                                                                                        AATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTT                                                            >  SRR19973073.2959610/1‑150 (MQ=60)
                                                                                         ATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTC                                                           >  SRR19973073.426762/1‑150 (MQ=60)
                                                                                           GTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTA                                                         <  SRR19973073.3236764/150‑1 (MQ=60)
                                                                                            TTCCTCTTCTTATTTTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTACATAAAGCGTTGACATTACTTTCTGTTCTAT                                                        <  SRR19973073.1081971/150‑1 (MQ=60)
                                                                                            TTACTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTAT                                                        <  SRR19973073.3058022/150‑1 (MQ=60)
                                                                                                 CTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGT                                                   >  SRR19973073.1316823/1‑150 (MQ=60)
                                                                                                    CTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGTTTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAAT                                                <  SRR19973073.3201782/150‑1 (MQ=60)
                                                                                                    CTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAAT                                                <  SRR19973073.2192607/150‑1 (MQ=60)
                                                                                                       ATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTC                                             <  SRR19973073.828118/150‑1 (MQ=60)
                                                                                                            ACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCC                                        <  SRR19973073.598538/150‑1 (MQ=60)
                                                                                                            ACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCC                                        <  SRR19973073.1804154/150‑1 (MQ=60)
                                                                                                                   TCCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAAC                                 <  SRR19973073.2530363/150‑1 (MQ=60)
                                                                                                                    CCAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACA                                <  SRR19973073.1873048/150‑1 (MQ=60)
                                                                                                                     CAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAA                               >  SRR19973073.1497109/1‑150 (MQ=60)
                                                                                                                     CAAAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAA                               >  SRR19973073.1496654/1‑150 (MQ=60)
                                                                                                                      AAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAAC                              >  SRR19973073.2680361/1‑150 (MQ=60)
                                                                                                                       AAGTAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACT                             >  SRR19973073.1710955/1‑150 (MQ=60)
                                                                                                                          TAATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAAT                          >  SRR19973073.366795/1‑150 (MQ=60)
                                                                                                                           AATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATT                         >  SRR19973073.1810250/1‑150 (MQ=60)
                                                                                                                           AATTTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATAACTTTCTGTTATATTAAGTAATTTCTCGCCGATAAACAACAAATT                         >  SRR19973073.1810253/1‑150 (MQ=60)
                                                                                                                             TTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTA                       <  SRR19973073.4238238/150‑1 (MQ=60)
                                                                                                                             TTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTA                       <  SRR19973073.4238224/150‑1 (MQ=60)
                                                                                                                             TTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTA                       >  SRR19973073.2859002/1‑150 (MQ=60)
                                                                                                                             TTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTAGTAAGTAATTTCTCGCCGATAAACAACAATTTTA                       >  SRR19973073.2859006/1‑150 (MQ=60)
                                                                                                                              TTTTCGAAGAGTAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTAT                      <  SRR19973073.3646719/150‑1 (MQ=60)
                                                                                                                              TTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTAT                      <  SRR19973073.3646729/150‑1 (MQ=60)
                                                                                                                              TTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTAT                      >  SRR19973073.2380783/1‑150 (MQ=60)
                                                                                                                              TTTACGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTAT                      <  SRR19973073.233154/150‑1 (MQ=60)
                                                                                                                                  CGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTATTGAT                  >  SRR19973073.39763/1‑150 (MQ=60)
                                                                                                                                  CGAAGAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTATTGAT                  >  SRR19973073.3973887/1‑150 (MQ=60)
                                                                                                                                     AGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTATTGATATT               <  SRR19973073.3384677/150‑1 (MQ=60)
                                                                                                                                      GAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTATTGATATTT              >  SRR19973073.778930/1‑150 (MQ=60)
                                                                                                                                      GAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTATTGATATTT              >  SRR19973073.778932/1‑150 (MQ=60)
                                                                                                                                      GAGAAATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAATAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTATTGATATTT              <  SRR19973073.2500125/150‑1 (MQ=60)
                                                                                                                                          AATAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTATTGATATTTAATA          >  SRR19973073.3419465/1‑150 (MQ=60)
                                                                                                                                            TAAGTGGATATAAGTGAAGTTAGTCACATAAAGAGATAGAAGATTTAGCTAAAAAAAGGGAAAAAACAGTACATAAAGCGTTGACATTACGTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTATTGATATTTAATAAA        >  SRR19973073.248104/1‑150 (MQ=60)
                                                                                                                                              AGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTATTGATATTTAATAAATT      >  SRR19973073.2822141/1‑150 (MQ=60)
                                                                                                                                                 GGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTATTGATATTTAATAAATTATT   <  SRR19973073.2326148/150‑1 (MQ=60)
                                                                                                                                                 GGATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTATTGATATTTAATAAATTATT   <  SRR19973073.2326245/150‑1 (MQ=60)
                                                                                                                                                  GATATAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTATTGATATTTAATAAATTATTG  <  SRR19973073.3220126/150‑1 (MQ=60)
                                                                                                                                                     |                                                                                                                                                  
CGCATGAGCAGCAAATGCAGCCATACCAGATGCCAGTATCAAAGATATCGAGGTACTTACGACACTTTTCTTTAATGCAGACTTCATAAATGTTCCTCTTCTTATTGTACTTAATTCCAAAGTAATTTTACGAAGAGAAATAAGTGGATGTAAGTGAAGTTAGTCACATAAAGAGATAGCAGATTTAGCTAAAAAAAGGGAAAAAACAGTCCATAAAGCGTTGACATTACTTTCTGTTCTATTAAGTAATTTCTCGCCGATAAACAACTAATTTATTGATATTTAATAAATTATTG  >  CP009273/1576695‑1576990

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑