Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F218 I0 R1 1506 278.7 9275112 94.5% 8764980 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,819,936 C→A 45.5% T456K (ACG→AAG)  recG → ATP‑dependent DNA helicase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,819,9360CA45.5% 242.6 / inf 285T456K (ACG→AAG) recGATP‑dependent DNA helicase
Reads supporting (aligned to +/- strand):  ref base C (92/63);  new base A (67/63);  total (159/126)
Fisher's exact test for biased strand distribution p-value = 1.91e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 285 total aligned reads displayed.

GTCTGGCATTGTGGGAGAAAGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAA  >  CP009273/3819789‑3820084
                                                                                                                                                   |                                                                                                                                                    
gTCTGGCATTGTGGGAGAAAGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGc                                                                                                                                                    >  2:754488/1‑150 (MQ=255)
    ggCATTGTGGGAGAAAGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGt                                                                                                                                                <  2:2944992/150‑1 (MQ=255)
     gCATTGTGGGAGAAAGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGACGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTg                                                                                                                                               <  2:2270591/150‑1 (MQ=255)
        ttGTGGGAGAAAGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACt                                                                                                                                            <  2:2774228/150‑1 (MQ=255)
        ttGTGGGAGAAAGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACt                                                                                                                                            <  1:3133493/150‑1 (MQ=255)
        ttGTGGGAGAAAGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACt                                                                                                                                            >  1:2116370/1‑150 (MQ=255)
         tgtgGGAGAAAGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTa                                                                                                                                           >  2:3510605/1‑150 (MQ=255)
           tgGGAGAAAGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACg                                                                                                                                         >  1:717301/1‑150 (MQ=255)
             ggAGAAAGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGt                                                                                                                                       >  1:3891962/1‑150 (MQ=255)
             ggAGAAAGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGt                                                                                                                                       <  1:2919057/150‑1 (MQ=255)
              gagaAAGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTc                                                                                                                                      >  1:1051296/1‑150 (MQ=255)
               agaAAGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCg                                                                                                                                     >  2:2352201/1‑150 (MQ=255)
                 aaaGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCt                                                                                                                                   <  1:3222159/150‑1 (MQ=255)
                  aaGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTa                                                                                                                                  >  2:1935385/1‑150 (MQ=255)
                      ccAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTcc                                                                                                                              <  1:945297/150‑1 (MQ=255)
                         gcagcagGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGa                                                                                                                           >  1:1212222/1‑150 (MQ=255)
                         gcagcagGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGa                                                                                                                           <  1:880663/150‑1 (MQ=255)
                          cagcagGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGAt                                                                                                                          <  1:3783496/150‑1 (MQ=255)
                           agcagGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATa                                                                                                                         <  1:3385199/150‑1 (MQ=255)
                           agcagGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATa                                                                                                                         >  1:330950/1‑150 (MQ=255)
                             cagGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATAcc                                                                                                                       >  1:2690937/1‑150 (MQ=255)
                              agGGCTTCCATTCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATAccc                                                                                                                      <  2:4024723/150‑1 (MQ=255)
                              agGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATAccc                                                                                                                      >  1:144344/1‑150 (MQ=255)
                                  cTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACccgccg                                                                                                                  >  2:4152441/1‑150 (MQ=255)
                                    tCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTa                                                                                                                >  2:1176730/1‑150 (MQ=255)
                                       aTCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCg                                                                                                             >  1:849495/1‑150 (MQ=255)
                                         ccGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGAc                                                                                                           <  1:180815/150‑1 (MQ=255)
                                         ccGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGAc                                                                                                           <  2:1126479/150‑1 (MQ=255)
                                            cATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGAcatc                                                                                                        <  2:40094/150‑1 (MQ=255)
                                             aTCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGAcatca                                                                                                       >  1:508192/1‑150 (MQ=255)
                                                aGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTg                                                                                                    >  2:3724517/1‑150 (MQ=255)
                                                   tGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGAcc                                                                                                 >  1:1515918/1‑150 (MQ=255)
                                                    gATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACcg                                                                                                >  1:2181075/1‑150 (MQ=255)
                                                      tCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACcgcg                                                                                              >  1:2583122/1‑150 (MQ=255)
                                                        aTGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTg                                                                                            >  2:444408/1‑150 (MQ=255)
                                                          gACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGca                                                                                          >  2:1436333/1‑150 (MQ=255)
                                                              gCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGcaccac                                                                                      >  2:2783399/1‑150 (MQ=255)
                                                               ccACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACg                                                                                     >  2:2840413/1‑150 (MQ=255)
                                                                cACGCCGATCCCCCACACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGc                                                                                    >  1:1155972/1‑150 (MQ=255)
                                                                    ccGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGc                                                                                <  1:1833890/150‑1 (MQ=255)
                                                                    ccGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGc                                                                                <  1:1382801/150‑1 (MQ=255)
                                                                     cGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCa                                                                               >  2:3203618/1‑150 (MQ=255)
                                                                     cGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCa                                                                               <  1:4304535/150‑1 (MQ=255)
                                                                      gATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCAt                                                                              >  1:2874945/1‑150 (MQ=255)
                                                                      gATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCAt                                                                              >  2:303176/1‑150 (MQ=255)
                                                                       aTCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATa                                                                             >  1:1871625/1‑150 (MQ=255)
                                                                       aTCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATa                                                                             >  2:208104/1‑150 (MQ=255)
                                                                          ccccGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACt                                                                          <  2:4605884/150‑1 (MQ=255)
                                                                           cccGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTg                                                                         <  2:3942342/150‑1 (MQ=255)
                                                                            ccGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACCTCATTGACCGCGTGCACCACGCCTGCATAACTGa                                                                        >  2:1495351/1‑150 (MQ=255)
                                                                               cACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAgg                                                                     >  1:1711923/1‑150 (MQ=255)
                                                                                aCGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGgt                                                                    <  2:3689471/150‑1 (MQ=255)
                                                                                    tGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGgtcgtc                                                                >  1:1116068/1‑150 (MQ=255)
                                                                                      gCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAg                                                              >  1:1923091/1‑150 (MQ=255)
                                                                                      gCAAAGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGATCTGCCGCCAGTCCGCACGCCAGTGACTACGGTCGCTATTACTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAg                                                              >  2:3056251/1‑150 (MQ=255)
                                                                                       cAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAgg                                                             <  1:3222789/150‑1 (MQ=255)
                                                                                       cAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAgg                                                             <  1:3623612/150‑1 (MQ=255)
                                                                                       cAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAgg                                                             >  2:33201/1‑150 (MQ=255)
                                                                                        aaTGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGc                                                            >  1:1731831/1‑150 (MQ=255)
                                                                                        aaTGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGc                                                            >  1:4150311/1‑150 (MQ=255)
                                                                                        aaTGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGc                                                            >  1:1763436/1‑150 (MQ=255)
                                                                                          tGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCAt                                                          >  1:3444325/1‑150 (MQ=255)
                                                                                           gACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATa                                                         <  1:4174853/150‑1 (MQ=255)
                                                                                             cTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACt                                                       >  2:3172560/1‑150 (MQ=255)
                                                                                               gCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTgg                                                     <  2:2287813/150‑1 (MQ=255)
                                                                                                 gTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCCGGCATACTGGGt                                                   >  1:4501697/1‑150 (MQ=255)
                                                                                                    tGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTg                                                <  1:2788116/150‑1 (MQ=255)
                                                                                                     gCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGGTTGt                                               >  2:2898053/1‑150 (MQ=255)
                                                                                                     gCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGt                                               >  1:1331200/1‑150 (MQ=255)
                                                                                                        gATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGAAACCCGCCGTACCGACAACATTGACCGCGTGCACCACGCCTGCATAACTTAAGGTCGTCAGGCAAACTGGGGTTGTaag                                            >  1:4523686/1‑148 (MQ=255)
                                                                                                          tctcGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGtt                                          >  2:2573604/1‑150 (MQ=255)
                                                                                                           ctcGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGttg                                         <  2:1394643/150‑1 (MQ=255)
                                                                                                           ctcGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGttg                                         >  2:2403072/1‑150 (MQ=255)
                                                                                                           ctcGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGttg                                         >  2:4272585/1‑150 (MQ=255)
                                                                                                            tcGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGttga                                        <  2:1216164/150‑1 (MQ=255)
                                                                                                            tcGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCAGATACCCGCCGTACCGACAACATAGACCGCGTTCACCACGCCTGCATAACTGAATGTCGTAAGGCATACTGGGTTTGTACGttga                                        >  1:4015272/1‑150 (MQ=255)
                                                                                                                tACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGttgattga                                    >  2:1865004/1‑150 (MQ=255)
                                                                                                                 aCCTCGGTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGaa                                   <  2:3813262/150‑1 (MQ=255)
                                                                                                                   cTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAaga                                 <  2:4408291/150‑1 (MQ=255)
                                                                                                                    tCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAagag                                <  1:998041/150‑1 (MQ=255)
                                                                                                                     cGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGt                               >  1:938003/1‑150 (MQ=255)
                                                                                                                      ggTGATAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTc                              <  1:2275274/150‑1 (MQ=255)
                                                                                                                       gTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCg                             >  1:2494114/1‑150 (MQ=255)
                                                                                                                        tGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCgg                            <  2:2150617/150‑1 (MQ=255)
                                                                                                                           tAGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAAt                         >  2:2213610/1‑150 (MQ=255)
                                                                                                                            aGATGAGCTGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAAtt                        <  2:1760386/150‑1 (MQ=255)
                                                                                                                             gATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTg                       >  2:583231/1‑150 (MQ=255)
                                                                                                                                gAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTg                    >  1:2364096/1‑150 (MQ=255)
                                                                                                                                  gCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGa                  >  1:1836368/1‑150 (MQ=255)
                                                                                                                                  gCTGCCGCAAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCTCGTGCACCACGCCTGAATAACTGAATGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGa                  <  2:4121048/150‑1 (MQ=255)
                                                                                                                                    tGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAg                >  1:3329086/1‑150 (MQ=255)
                                                                                                                                    tGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCAGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAg                >  2:2517952/1‑150 (MQ=255)
                                                                                                                                    tGCCGCCAGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGAAACCCGCCGGACCCACATCATTTACCGCGTGCACCACGCCTGCATAACAGAAGGTCGTCAGGCATACTGGGTTTGTAGGTTTTTTTAAGAGTCGGTATTGGTGGAAg                >  1:1010543/1‑150 (MQ=255)
                                                                                                                                       cgccAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACAGAAGGTCGTCAGGCATACTGGGGTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAgcgc             >  1:2025178/1‑150 (MQ=255)
                                                                                                                                           aGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAggc         <  1:754488/150‑1 (MQ=255)
                                                                                                                                           aGGCCGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAggc         >  2:3551/1‑150 (MQ=255)
                                                                                                                                             gCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAggcgg       >  2:3785897/1‑150 (MQ=255)
                                                                                                                                              ccGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAggcggc      >  2:2649066/1‑150 (MQ=255)
                                                                                                                                               cGCAAGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGgcggcg     >  2:2296223/1‑150 (MQ=255)
                                                                                                                                                  aCGCCAGTGACTACGGTCGCTATTACTGATACCCCCCGTACCGACCTCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGaa  >  1:2320053/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                    
GTCTGGCATTGTGGGAGAAAGGCCAGCAGCAGGGCTTCCATCCGCATCAGTTGATCATGACCGCCACGCCGATCCCCCGCACGCTGGCAATGACTGCGTATGCCGATCTCGATACCTCGGTGATAGATGAGCTGCCGCCAGGCCGCACGCCAGTGACTACGGTCGCTATTCCTGATACCCGCCGTACCGACATCATTGACCGCGTGCACCACGCCTGCATAACTGAAGGTCGTCAGGCATACTGGGTTTGTACGTTGATTGAAGAGTCGGAATTGCTGGAAGCGCAGGCGGCGGAA  >  CP009273/3819789‑3820084

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A