Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,330,955 G→T 100% D290Y (GAT→TAT)  acnA → aconitate hydratase 1

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,330,9550GT99.5% 646.8 / ‑4.1 200D290Y (GAT→TAT) acnAaconitate hydratase 1
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (107/92);  total (107/93)
Fisher's exact test for biased strand distribution p-value = 4.65e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.82e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 209 total aligned reads displayed.

CCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCG  >  CP009273/1330808‑1331099
                                                                                                                                                   |                                                                                                                                                
ccGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTAAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTg                                                                                                                                                  <  1:1580145/148‑1 (MQ=255)
  ggTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTAt                                                                                                                                                >  1:3439591/1‑148 (MQ=255)
    tttCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTttatggttatgg                                                                                                                                              >  2:1429960/1‑148 (MQ=255)
    tttCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTttatggttatgg                                                                                                                                              >  1:1523670/1‑148 (MQ=255)
     ttCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTtatggttatggt                                                                                                                                             >  1:2648216/1‑148 (MQ=255)
         aTGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTgg                                                                                                                                         >  2:1252280/1‑148 (MQ=255)
         aTGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTgg                                                                                                                                         >  2:3819226/1‑148 (MQ=255)
          tGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGa                                                                                                                                        <  1:2062852/148‑1 (MQ=255)
            cTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGAtt                                                                                                                                      <  1:1755570/148‑1 (MQ=255)
                 cccGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTa                                                                                                                                 >  2:867418/1‑148 (MQ=255)
                 cccGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTa                                                                                                                                 <  2:1223904/148‑1 (MQ=255)
                 cccGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTa                                                                                                                                 <  2:3128294/148‑1 (MQ=255)
                 cccGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTa                                                                                                                                 >  1:1244294/1‑148 (MQ=255)
                    ggATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCg                                                                                                                              >  1:1819022/1‑148 (MQ=255)
                    ggATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCg                                                                                                                              >  1:1819034/1‑148 (MQ=255)
                    ggATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCg                                                                                                                              <  1:2143010/148‑1 (MQ=255)
                     gATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGt                                                                                                                             >  2:2131460/1‑148 (MQ=255)
                        gTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTgg                                                                                                                          <  2:3004582/148‑1 (MQ=255)
                        gTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTgg                                                                                                                          >  2:320033/1‑148 (MQ=255)
                           gTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCgg                                                                                                                       >  2:84635/1‑148 (MQ=255)
                           gTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCgg                                                                                                                       >  1:584503/1‑148 (MQ=255)
                            tGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGa                                                                                                                      >  1:1001109/1‑148 (MQ=255)
                            tGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGa                                                                                                                      <  1:65715/148‑1 (MQ=255)
                            tGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGa                                                                                                                      <  1:1325586/148‑1 (MQ=255)
                            tGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGa                                                                                                                      >  1:847879/1‑148 (MQ=255)
                            tGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGa                                                                                                                      <  1:1526454/148‑1 (MQ=255)
                            tGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGa                                                                                                                      >  1:1983775/1‑148 (MQ=255)
                                 ttCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATcgcg                                                                                                                 <  2:1691071/148‑1 (MQ=255)
                                  tCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCgcgc                                                                                                                >  2:1933836/1‑148 (MQ=255)
                                  tCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCgcgc                                                                                                                <  1:2729794/148‑1 (MQ=255)
                                   cAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGcc                                                                                                               >  2:3304738/1‑148 (MQ=255)
                                     aaCTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGccac                                                                                                             <  1:2719427/148‑1 (MQ=255)
                                      aCTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGccacc                                                                                                            <  1:3266267/148‑1 (MQ=255)
                                      aCTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGccacc                                                                                                            <  2:1489156/148‑1 (MQ=255)
                                       cTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGccacca                                                                                                           >  2:474254/1‑148 (MQ=255)
                                       cTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGccacca                                                                                                           >  1:746453/1‑148 (MQ=255)
                                       cTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGccacca                                                                                                           <  2:2705440/148‑1 (MQ=255)
                                       cTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGccacca                                                                                                           >  1:2897360/1‑148 (MQ=255)
                                        ttACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCAt                                                                                                          <  1:3714884/148‑1 (MQ=255)
                                         tACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCAtt                                                                                                         >  1:661535/1‑148 (MQ=255)
                                          aCCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTg                                                                                                        >  1:808776/1‑148 (MQ=255)
                                             ggACAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAAGTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCa                                                                                                     <  2:1391939/148‑1 (MQ=255)
                                              gAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCaa                                                                                                    >  2:2849212/1‑148 (MQ=255)
                                               aaaaTTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAat                                                                                                   >  1:1309979/1‑148 (MQ=255)
                                                aaaTTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAata                                                                                                  <  1:2098414/148‑1 (MQ=255)
                                                aaaTTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAata                                                                                                  >  2:2475095/1‑148 (MQ=255)
                                                   ttACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGt                                                                                               <  1:930913/148‑1 (MQ=255)
                                                    tACGTGAAGGTATTACCGCCACAGACCTGGGTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAAGTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTc                                                                                              <  2:3230744/148‑1 (MQ=255)
                                                          aaGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGa                                                                                        <  1:2400645/148‑1 (MQ=255)
                                                          aaGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGa                                                                                        <  1:2410612/148‑1 (MQ=255)
                                                           aGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGaa                                                                                       <  1:2849212/148‑1 (MQ=255)
                                                             gTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAata                                                                                     >  1:116325/1‑148 (MQ=255)
                                                              tATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAatat                                                                                    <  2:445250/148‑1 (MQ=255)
                                                                 tACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGt                                                                                 >  1:3442573/1‑148 (MQ=255)
                                                                      ccACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCAc                                                                            >  2:553275/1‑148 (MQ=255)
                                                                       cacaGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCAcc                                                                           >  1:2243780/1‑148 (MQ=255)
                                                                       cacaGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCAcc                                                                           >  1:316717/1‑148 (MQ=255)
                                                                         caGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCtg                                                                         >  1:3018902/1‑148 (MQ=255)
                                                                          aGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCtgt                                                                        <  2:2586446/148‑1 (MQ=255)
                                                                                ggTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGcttc                                                                  >  1:1615198/1‑148 (MQ=255)
                                                                                 gTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGcttct                                                                 >  2:560029/1‑148 (MQ=255)
                                                                                 gTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGcttct                                                                 >  2:803306/1‑148 (MQ=255)
                                                                                  ttCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGcttctt                                                                >  2:3386743/1‑148 (MQ=255)
                                                                                   tctcACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCttcttc                                                               >  2:1696807/1‑148 (MQ=255)
                                                                                    ctcACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTcc                                                              <  2:2401324/148‑1 (MQ=255)
                                                                                      cACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCa                                                            <  2:739063/148‑1 (MQ=255)
                                                                                      cACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCa                                                            >  1:1544870/1‑148 (MQ=255)
                                                                                      cACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCa                                                            <  2:1115831/148‑1 (MQ=255)
                                                                                        cTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAAt                                                          <  1:3502217/148‑1 (MQ=255)
                                                                                         tGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATc                                                         >  1:1796917/1‑148 (MQ=255)
                                                                                            tACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGAt                                                      <  2:3341313/148‑1 (MQ=255)
                                                                                            tACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGAt                                                      <  2:3340660/148‑1 (MQ=255)
                                                                                                 aaaTGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGt                                                 >  1:1222279/1‑148 (MQ=255)
                                                                                                 aaaTGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGt                                                 >  1:3807905/1‑148 (MQ=255)
                                                                                                  aaTGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTa                                                <  1:2684973/148‑1 (MQ=255)
                                                                                                   aTGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTaa                                               <  1:2278411/148‑1 (MQ=255)
                                                                                                           cAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGAt                                       >  2:3446627/1‑148 (MQ=255)
                                                                                                           cAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGAt                                       >  2:3447507/1‑148 (MQ=255)
                                                                                                            aaaCATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGAtt                                      >  2:2622384/1‑148 (MQ=255)
                                                                                                             aaCATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTa                                     >  1:2034279/1‑148 (MQ=255)
                                                                                                              aCATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTAc                                    >  2:542680/1‑148 (MQ=255)
                                                                                                                 tGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATg                                 >  2:3718249/1‑148 (MQ=255)
                                                                                                                        gtggGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTaa                          <  2:1742358/148‑1 (MQ=255)
                                                                                                                         tggGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAg                         <  1:563993/148‑1 (MQ=255)
                                                                                                                          ggggAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGc                        >  2:1580906/1‑148 (MQ=255)
                                                                                                                          ggggAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGc                        <  2:357991/148‑1 (MQ=255)
                                                                                                                                aTTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGgcgc                  >  2:2879854/1‑148 (MQ=255)
                                                                                                                                aTTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGgcgc                  >  1:2191842/1‑148 (MQ=255)
                                                                                                                                  tcgtcgAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAg                <  1:1193463/148‑1 (MQ=255)
                                                                                                                                  tcgtcgAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAg                >  2:2282164/1‑148 (MQ=255)
                                                                                                                                     tcgAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGa             >  2:2036419/1‑148 (MQ=255)
                                                                                                                                     tcgAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGa             <  1:2817417/148‑1 (MQ=255)
                                                                                                                                        aaTTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGa          <  2:1151339/148‑1 (MQ=255)
                                                                                                                                         aTTTTATGGTTATTGTCTGGAGTCACTACCGTTGGAGGATCGCGACACCATTGCCAATATGTCGCCAGAATATGGTGCCTCCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGatgtt         <  2:2189960/148‑5 (MQ=255)
                                                                                                                                           tttATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCa       >  1:3054189/1‑148 (MQ=255)
                                                                                                                                             tatggttatggtCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAgg     <  1:3121985/148‑1 (MQ=255)
                                                                                                                                              aTGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGt    <  1:553275/148‑1 (MQ=255)
                                                                                                                                              aTGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGt    <  1:2753870/148‑1 (MQ=255)
                                                                                                                                              aTGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGt    <  1:2473589/148‑1 (MQ=255)
                                                                                                                                                ggTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCg  >  2:1030992/1‑148 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCG  >  CP009273/1330808‑1331099

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTG  >  CP009273/1330808‑1331104
                                                                                                                                                   |                                                                                                                                                     
CCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTAT                                                                                                                                                     >  SRR19973069.3461925/1‑150 (MQ=60)
CCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTAT                                                                                                                                                     >  SRR19973069.3462075/1‑150 (MQ=60)
CCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTAAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGAT                                                                                                                                                     <  SRR19973069.1590869/150‑1 (MQ=60)
 CGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATG                                                                                                                                                    <  SRR19973069.2911029/150‑1 (MQ=60)
 CGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATG                                                                                                                                                    <  SRR19973069.2911034/150‑1 (MQ=60)
 CGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATG                                                                                                                                                    <  SRR19973069.3691647/150‑1 (MQ=60)
  GGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGG                                                                                                                                                   >  SRR19973069.908350/1‑150 (MQ=60)
  GGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGG                                                                                                                                                   >  SRR19973069.1534167/1‑150 (MQ=60)
   GTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGT                                                                                                                                                  >  SRR19973069.2665954/1‑150 (MQ=60)
       CCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGG                                                                                                                                              >  SRR19973069.1159369/1‑150 (MQ=60)
         ATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGAT                                                                                                                                            >  SRR19973069.2407466/1‑150 (MQ=60)
         ATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGAT                                                                                                                                            <  SRR19973069.1242979/150‑1 (MQ=60)
         ATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGAT                                                                                                                                            <  SRR19973069.1702573/150‑1 (MQ=60)
          TGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATT                                                                                                                                           <  SRR19973069.2077155/150‑1 (MQ=60)
            CTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCA                                                                                                                                         <  SRR19973069.1767936/150‑1 (MQ=60)
              TATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACT                                                                                                                                       >  SRR19973069.814862/1‑150 (MQ=60)
               ATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTA                                                                                                                                      >  SRR19973069.1252744/1‑150 (MQ=60)
                  CCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCG                                                                                                                                   >  SRR19973069.1831803/1‑150 (MQ=60)
                  CCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCG                                                                                                                                   >  SRR19973069.1831799/1‑150 (MQ=60)
                  CCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCG                                                                                                                                   >  SRR19973069.1831790/1‑150 (MQ=60)
                  CCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCG                                                                                                                                   >  SRR19973069.1831739/1‑150 (MQ=60)
                    GGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTT                                                                                                                                 <  SRR19973069.2157617/150‑1 (MQ=60)
                    GGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTT                                                                                                                                 <  SRR19973069.2157724/150‑1 (MQ=60)
                      ATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGG                                                                                                                               >  SRR19973069.2260820/1‑150 (MQ=60)
                      ATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGG                                                                                                                               >  SRR19973069.765824/1‑150 (MQ=60)
                      ATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGG                                                                                                                               >  SRR19973069.2260899/1‑150 (MQ=60)
                         TAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGG                                                                                                                            >  SRR19973069.588504/1‑150 (MQ=60)
                          AGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGA                                                                                                                           >  SRR19973069.853822/1‑150 (MQ=60)
                          AGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGA                                                                                                                           >  SRR19973069.1007974/1‑150 (MQ=60)
                          AGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGA                                                                                                                           >  SRR19973069.1997578/1‑150 (MQ=60)
                           GTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGAT                                                                                                                          >  SRR19973069.671121/1‑150 (MQ=60)
                            TGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATC                                                                                                                         >  SRR19973069.2417577/1‑150 (MQ=60)
                            TGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATC                                                                                                                         <  SRR19973069.1334695/150‑1 (MQ=60)
                            TGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATC                                                                                                                         <  SRR19973069.1334701/150‑1 (MQ=60)
                            TGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATC                                                                                                                         <  SRR19973069.66478/150‑1 (MQ=60)
                            TGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATC                                                                                                                         <  SRR19973069.1536961/150‑1 (MQ=60)
                            TGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATC                                                                                                                         <  SRR19973069.1536963/150‑1 (MQ=60)
                                  TCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCA                                                                                                                   <  SRR19973069.2748074/150‑1 (MQ=60)
                                  TCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTACTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCA                                                                                                                   <  SRR19973069.2748472/150‑1 (MQ=60)
                                     AACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCA                                                                                                                <  SRR19973069.2737166/150‑1 (MQ=60)
                                     AACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCA                                                                                                                <  SRR19973069.2737648/150‑1 (MQ=60)
                                     AACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCA                                                                                                                >  SRR19973069.751743/1‑150 (MQ=60)
                                     AACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCA                                                                                                                >  SRR19973069.2916480/1‑150 (MQ=60)
                                      ACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCAT                                                                                                               <  SRR19973069.3287659/150‑1 (MQ=60)
                                       CTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATT                                                                                                              >  SRR19973069.666008/1‑150 (MQ=60)
                                       CTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATT                                                                                                              >  SRR19973069.3163921/1‑150 (MQ=60)
                                       CTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATT                                                                                                              >  SRR19973069.665999/1‑150 (MQ=60)
                                        TTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTG                                                                                                             <  SRR19973069.287438/150‑1 (MQ=60)
                                        TTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTG                                                                                                             <  SRR19973069.3739143/150‑1 (MQ=60)
                                        TTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTG                                                                                                             <  SRR19973069.3737042/150‑1 (MQ=60)
                                        TTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTG                                                                                                             >  SRR19973069.2743238/1‑150 (MQ=60)
                                        TTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTG                                                                                                             >  SRR19973069.814501/1‑150 (MQ=60)
                                             GGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAAT                                                                                                        <  SRR19973069.2071669/150‑1 (MQ=60)
                                             GGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAAT                                                                                                        >  SRR19973069.1318934/1‑150 (MQ=60)
                                              GAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATA                                                                                                       >  SRR19973069.184789/1‑150 (MQ=60)
                                                AAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATG                                                                                                     <  SRR19973069.2112907/150‑1 (MQ=60)
                                                 AATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGT                                                                                                    >  SRR19973069.3474346/1‑150 (MQ=60)
                                                   TTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCG                                                                                                  <  SRR19973069.937229/150‑1 (MQ=60)
                                                          AAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAAT                                                                                           <  SRR19973069.2082887/150‑1 (MQ=60)
                                                          AAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAAT                                                                                           <  SRR19973069.2416895/150‑1 (MQ=60)
                                                          AAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAAT                                                                                           <  SRR19973069.2426909/150‑1 (MQ=60)
                                                           AGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATA                                                                                          >  SRR19973069.117454/1‑150 (MQ=60)
                                                           AGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATA                                                                                          <  SRR19973069.2867980/150‑1 (MQ=60)
                                                               ATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGT                                                                                      >  SRR19973069.3464920/1‑150 (MQ=60)
                                                                 TACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGC                                                                                    >  SRR19973069.3069030/1‑150 (MQ=60)
                                                                  ACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCC                                                                                   <  SRR19973069.1591628/150‑1 (MQ=60)
                                                                     GCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACC                                                                                >  SRR19973069.319003/1‑150 (MQ=60)
                                                                     GCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACC                                                                                >  SRR19973069.318807/1‑150 (MQ=60)
                                                                     GCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACC                                                                                >  SRR19973069.2258894/1‑150 (MQ=60)
                                                                       CACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTG                                                                              >  SRR19973069.3038929/1‑150 (MQ=60)
                                                                          AGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGG                                                                           >  SRR19973069.3130820/1‑150 (MQ=60)
                                                                            ACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCT                                                                         <  SRR19973069.2999592/150‑1 (MQ=60)
                                                                              CTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTC                                                                       >  SRR19973069.1626091/1‑150 (MQ=60)
                                                                              CTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTC                                                                       >  SRR19973069.2663824/1‑150 (MQ=60)
                                                                                    CTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCA                                                                 >  SRR19973069.1555448/1‑150 (MQ=60)
                                                                                    CTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCA                                                                 >  SRR19973069.1410881/1‑150 (MQ=60)
                                                                                     TCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAA                                                                >  SRR19973069.1174280/1‑150 (MQ=60)
                                                                                       ACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATC                                                              >  SRR19973069.1809385/1‑150 (MQ=60)
                                                                                       ACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCACAAAC                                                              >  SRR19973069.1809570/1‑150 (MQ=60)
                                                                                        CTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCG                                                             <  SRR19973069.3524765/150‑1 (MQ=60)
                                                                                         TGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGA                                                            >  SRR19973069.2185438/1‑150 (MQ=60)
                                                                                            TACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGC                                                         <  SRR19973069.2032870/150‑1 (MQ=60)
                                                                                               CCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGT                                                      >  SRR19973069.3832557/1‑150 (MQ=60)
                                                                                               CCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGT                                                      >  SRR19973069.1230634/1‑150 (MQ=60)
                                                                                               CCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGT                                                      >  SRR19973069.1578029/1‑150 (MQ=60)
                                                                                                  AATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAAC                                                   <  SRR19973069.2702966/150‑1 (MQ=60)
                                                                                                   ATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACC                                                  <  SRR19973069.2293877/150‑1 (MQ=60)
                                                                                                         CGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGAT                                            >  SRR19973069.2878067/1‑150 (MQ=60)
                                                                                                         CGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGAT                                            <  SRR19973069.644649/150‑1 (MQ=60)
                                                                                                          GCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATT                                           <  SRR19973069.154396/150‑1 (MQ=60)
                                                                                                           CAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTA                                          >  SRR19973069.2048400/1‑150 (MQ=60)
                                                                                                             AACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCCATGCTGTAACCCTCGATTACA                                        >  SRR19973069.2667107/1‑150 (MQ=60)
                                                                                                                     GTGGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTA                                <  SRR19973069.1255370/150‑1 (MQ=60)
                                                                                                                       GGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAG                              <  SRR19973069.380551/150‑1 (MQ=60)
                                                                                                                       GGTGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAG                              <  SRR19973069.3408861/150‑1 (MQ=60)
                                                                                                                         TGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCG                            <  SRR19973069.2476170/150‑1 (MQ=60)
                                                                                                                         TGGGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCG                            <  SRR19973069.567921/150‑1 (MQ=60)
                                                                                                                           GGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGG                          <  SRR19973069.1704608/150‑1 (MQ=60)
                                                                                                                           GGGAAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGG                          <  SRR19973069.2215025/150‑1 (MQ=60)
                                                                                                                              AAATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGC                       >  SRR19973069.2206752/1‑150 (MQ=60)
                                                                                                                                ATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAG                     >  SRR19973069.581265/1‑150 (MQ=60)
                                                                                                                                ATTCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAG                     >  SRR19973069.2850913/1‑150 (MQ=60)
                                                                                                                                  TCGTCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCG                   <  SRR19973069.1201682/150‑1 (MQ=60)
                                                                                                                                     TCGAATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAG                <  SRR19973069.2836070/150‑1 (MQ=60)
                                                                                                                                         ATTTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCA            >  SRR19973069.3074396/1‑150 (MQ=60)
                                                                                                                                           TTTATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGG          <  SRR19973069.476529/150‑1 (MQ=60)
                                                                                                                                             TATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTC        <  SRR19973069.3142492/150‑1 (MQ=60)
                                                                                                                                             TATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTC        <  SRR19973069.1226122/150‑1 (MQ=60)
                                                                                                                                              ATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCG       <  SRR19973069.2822754/150‑1 (MQ=60)
                                                                                                                                              ATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCG       <  SRR19973069.2772251/150‑1 (MQ=60)
                                                                                                                                              ATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCG       <  SRR19973069.558059/150‑1 (MQ=60)
                                                                                                                                              ATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCG       <  SRR19973069.557136/150‑1 (MQ=60)
                                                                                                                                              ATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCG       <  SRR19973069.521572/150‑1 (MQ=60)
                                                                                                                                              ATGGTTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCG       <  SRR19973069.2490177/150‑1 (MQ=60)
                                                                                                                                                  TTATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTT   <  SRR19973069.239735/150‑1 (MQ=60)
                                                                                                                                                   TATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTG  <  SRR19973069.1439947/150‑1 (MQ=60)
                                                                                                                                                   TATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTG  <  SRR19973069.2581176/150‑1 (MQ=60)
                                                                                                                                                   |                                                                                                                                                     
CCGGTTTCCATGCTTATCCCGGATGTAGTGGGCTTCAAACTTACCGGAAAATTACGTGAAGGTATTACCGCCACAGACCTGGTTCTCACTGTTACCCAAATGCTGCGCAAACATGGCGTGGTGGGGAAATTCGTCGAATTTTATGGTGATGGTCTGGATTCACTACCGTTGGCGGATCGCGCCACCATTGCCAATATGTCGCCAGAATATGGTGCCACCTGTGGCTTCTTCCCAATCGATGCTGTAACCCTCGATTACATGCGTTTAAGCGGGCGCAGCGAAGATCAGGTCGAGTTG  >  CP009273/1330808‑1331104

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑