Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.

ALE, Flask, Isolate	Predicted Mutations	Mean Coverage	Total Reads	Percent Mapped	Mapped Reads	Average Read Length
A1 F342 I0 R1	1331	225.9	7641038	95.8%	7320114	147.9

Breseq alignment

BRESEQ :: Evidence

Predicted mutation
evidence	seq id	position	mutation	freq	annotation	gene	description
RA	CP009273	1,437,184	G→T	21.8%	intergenic (‑84/‑124)	ldhA ← / → ydbH	fermentative D‑lactate dehydrogenase, NAD‑dependent/putative membrane‑anchored protein, function unknown

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	CP009273	1,437,184	0	G	T	21.8%	705.0 / 139.0	278	intergenic (‑84/‑124)	ldhA/ydbH	fermentative D‑lactate dehydrogenase, NAD‑dependent/putative membrane‑anchored protein, function unknown
Reads supporting (aligned to +/- strand): ref base G (113/104); new base T (35/26); total (148/130)
Fisher's exact test for biased strand distribution p-value = 4.72e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.02e-01

Only 100 of 279 total aligned reads displayed.

ACTCGTTCACCTGTTGCAGGTACTTCTTGTCGTACTGTTTTGTGCTATAAACGGCGAGTTTCATAAGACTTTCTCCAGTGATGTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCTTAATTTTTCGCTAAATCAGGATATTAACTACCCATGCTGGGTAAATATAAAGCCG  >  CP009273/1437037‑1437329

   cGTTCACCTGTTGCAGGTACTTCTTGTCGTACTGTTTTGTGCTATAAACGGCGAGTTTCATAAGACTTTCTCCAGTGATGTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGata                                                                                                                                                <  1:2207334/148‑1 (MQ=255)

   cGTTCACCTGTTGCAGGTACTTCTTGTCGTACTGTTTTGTGCTATAAACGGCGAGTTTCATAAGACTTTCTCCAGTGATGTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGata                                                                                                                                                <  2:803134/148‑1 (MQ=255)

       cACCTGTTGCAGGTACTTCTTGTCGTACTGTTTTGTGCTATAAACGGCGAGTTTCATAAGACTTTCTCCAGTGATGTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGATATTGCGATATTGa                                                                                                                                            >  2:3033780/1‑148 (MQ=255)

           tGTTGAAGTTACTTCTTGTCGTACTTTTTTGTGATATAAACGGCGAGTTTCATAAGACTTTCTCCAGTGATGTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGaaaaa                                                                                                                                        <  2:3671709/148‑1 (MQ=255)

            gTTGCAGGTACTTCTTGTCGTACTGTTTTGTGCTATAAACGGCGAGTTTCATAAGACTTTCTCCAGTGATGTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAAt                                                                                                                                       >  1:804132/1‑148 (MQ=255)

                cAGGTACTTCTTGTCGTACTGTTTTGTGCTAAAAACGGCGAGTTTCATAAGACTTTCTCCAGTGATGTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAACTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATtcat                                                                                                                                   <  1:4340/148‑1 (MQ=255)

                 aGGTACTTCTTGTCGTACTGTTTTGTGCTATAAACGGCGAGTTTCATAAGACTTTCTCCAGTGATGTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATtcatc                                                                                                                                  <  2:15978/148‑1 (MQ=255)

                  ggTACTTCTTGTCGTACTGTTTTGTGCTATAAACGGCGAGTTTCATAAGACTTTCTCCAGTGATGTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATtcatca                                                                                                                                 <  2:3555156/148‑1 (MQ=255)

                   gTACTTCTTGTCGTACTGTTTTGTGCTATAAACGGCGAGTTTCATAAGACTTTCTCCAGTGATGTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATcaa                                                                                                                                >  1:1912577/1‑148 (MQ=255)

                      cttcttGTCGTACTGTTTTGTGCTATAAACGGCGAGTTTCATAAGACTTTCTCCAGTGATGTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATcaacaa                                                                                                                             <  1:2956437/148‑1 (MQ=255)

                       ttcttGTCGTACTGTTTTGTGCTATAAACGGCGAGTTTCATAAGACTTTCTCCAGTGATGTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATAaacaac                                                                                                                            >  2:334103/1‑148 (MQ=255)

                        tcttGTCGTACTGTTTTGTGCTATAAACGGCGAGTTTCATAAGACTTTCTCCAGTGATGTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACt                                                                                                                           >  1:3121901/1‑148 (MQ=255)

                         cttGTCGTACTGTTTTGTGCTATAAACGGCGAGTTTCATAAGACTTTCTCCAGTGATGTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTa                                                                                                                          <  1:2245162/148‑1 (MQ=255)

                           tGTCGTACTGTTTTGTGCTATAAACGGCGAGTTTCATAAGACTTTCTCCAGTGATGTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCTATATTGAAAAATTCATCAACAACTATg                                                                                                                        >  2:3383914/1‑148 (MQ=255)

                           tGTCGTACTGTTTTGTGCTATAAACGGCGAGTTTCATAAGACTTTCTCCAGTGATGTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATg                                                                                                                        <  1:652590/148‑1 (MQ=255)

                           tGTCGTACTGTTTTGTGCTATAAACGGCGAGTTTCATAAGACTTTCTCCAGTGATGTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATg                                                                                                                        >  1:1707533/1‑148 (MQ=255)

                              cGTACTGTTTTGTGCTATAAACGGCGAGTTTCATAAGACTTTCTCCAGTGATGTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCtt                                                                                                                     >  1:1658734/1‑148 (MQ=255)

                                tACTGTTTTGTGCTATAAACGGCGAGTTTCATAAGACTTTCTCCAGTGATGTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAg                                                                                                                   >  2:1337146/1‑148 (MQ=255)

                                 aCTGTTTTGTGCTATAAACGGCGAGTTTCATAAGACTTTCTCCAGTGATGTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCTATATTGAAAAATTCATCAACAACTATGCTTAgt                                                                                                                  >  2:1880252/1‑148 (MQ=255)

                                  cTGTTTTGTGCTATAAACGGCGAGTTTCATAAGACTTTCTCCAGTGATGTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAgtg                                                                                                                 >  2:1386656/1‑148 (MQ=255)

                                   tGTTTTGTGCTATAAACGGCGAGTTTCATAAGACTTTCTCCAGTGATGTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCTATATTGAAAAATTCATCAACAACTATGCTTAgtgt                                                                                                                <  2:1101888/148‑1 (MQ=255)

                                      tttGTGCTATAAACGGCGAGTTTCATAAGACTTTCTCCAGTGATGTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCTATATTGAAAAATTCATCAACAACTATGCTTAGTGTAgg                                                                                                             >  2:1427114/1‑148 (MQ=255)

                                       ttGTGCTATAAACGGCGAGTTTCATAAGACTTTCTCCAGTGATGTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGgc                                                                                                            >  1:982400/1‑148 (MQ=255)

                                              ataAACGGCGAGTTTCATAAGACTTTCTCCAGTGATGTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCt                                                                                                     >  1:1563586/1‑148 (MQ=255)

                                              ataAACGGCGAGTTTCATAAGACTTTCTCCAGTGATGTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCt                                                                                                     >  2:119727/1‑148 (MQ=255)

                                                aaaCGGCGAGTTTCATAAGACTTTCTCCAGTGATGTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCTATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTc                                                                                                   >  1:302287/1‑148 (MQ=255)

                                                 caCGGCGAGTTTCATAAGACTTTCTCCAGTGATGTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCa                                                                                                  <  1:3564821/147‑1 (MQ=255)

                                                  aCGGCGAGTTTCATAAGACTTTCTCCAGTGATGTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCaa                                                                                                 >  1:2167604/1‑148 (MQ=255)

                                                           ttCATAAGACTTTCTCCAGTGATGTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCTATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGt                                                                                        <  1:1163286/148‑1 (MQ=255)

                                                               tAAGACTTTCTCCAGTGATGTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTaaa                                                                                    <  1:2734735/148‑1 (MQ=255)

                                                                 aGACTTTCTCCAGTGATGTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACa                                                                                  >  2:3167896/1‑148 (MQ=255)

                                                                 aGACTTTCTCCAGTGATGTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACa                                                                                  <  1:5337/148‑1 (MQ=255)

                                                                  gACTTTCTCCAGTGATGTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACAt                                                                                 >  2:2645071/1‑148 (MQ=255)

                                                                     tttCTCCAGTGATGTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGcc                                                                              >  2:2359262/1‑148 (MQ=255)

                                                                      ttCTCCAGTGATGTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGACCGGTTAAACATGCca                                                                             >  1:361206/1‑148 (MQ=255)

                                                                          ccAGTGATGTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAAt                                                                         >  1:2465795/1‑148 (MQ=255)

                                                                          ccAGTGATGTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAAt                                                                         >  1:2120071/1‑148 (MQ=255)

                                                                            aGTGATGTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATAc                                                                       <  1:119440/148‑1 (MQ=255)

                                                                             gTGATGTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCTATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATAcc                                                                      <  2:1156835/148‑1 (MQ=255)

                                                                                aTGTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCTATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGt                                                                   >  1:2377669/1‑148 (MQ=255)

                                                                                aTGTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCTATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGt                                                                   >  1:1079271/1‑148 (MQ=255)

                                                                                 tGTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTa                                                                  <  2:320647/148‑1 (MQ=255)

                                                                                  gTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTAt                                                                 <  2:1172621/148‑1 (MQ=255)

                                                                                    tGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCTATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTg                                                               >  1:1003131/1‑148 (MQ=255)

                                                                                     gAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGa                                                              <  2:1023086/148‑1 (MQ=255)

                                                                                     gAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGa                                                              <  2:547652/148‑1 (MQ=255)

                                                                                      aaTCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGaa                                                             <  2:776341/148‑1 (MQ=255)

                                                                                      aaTCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGCATTGaa                                                             <  2:589735/148‑1 (MQ=255)

                                                                                        tCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCTATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATg                                                           <  1:1003431/148‑1 (MQ=255)

                                                                                        tCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATg                                                           >  1:381533/1‑148 (MQ=255)

                                                                                        tCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATg                                                           >  1:263951/1‑148 (MQ=255)

                                                                                         cacaTTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGc                                                          <  2:383786/148‑1 (MQ=255)

                                                                                          acaTTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCt                                                         <  2:932626/148‑1 (MQ=255)

                                                                                          acaTTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTAATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCt                                                         >  1:1872051/1‑148 (MQ=255)

                                                                                           caTTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCtt                                                        >  1:1489410/1‑148 (MQ=255)

                                                                                           caTTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCtt                                                        <  2:2872476/148‑1 (MQ=255)

                                                                                             tttAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCTTaa                                                      <  2:2168779/148‑1 (MQ=255)

                                                                                             tttAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCTTaa                                                      <  2:714044/148‑1 (MQ=255)

                                                                                             tttAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCTTaa                                                      <  1:2899565/148‑1 (MQ=255)

                                                                                             tttAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCTTaa                                                      >  2:3131069/1‑148 (MQ=255)

                                                                                             tttAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCTTaa                                                      <  1:2414628/148‑1 (MQ=255)

                                                                                              ttAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCTATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCTTAAt                                                     >  2:2833607/1‑148 (MQ=255)

                                                                                              ttAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCTTAAt                                                     >  1:13574/1‑148 (MQ=255)

                                                                                                aaGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCTATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCTTAAttt                                                   >  2:408174/1‑148 (MQ=255)

                                                                                                aaGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCTTAAttt                                                   >  1:1299966/1‑148 (MQ=255)

                                                                                                  gCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCTATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCTTAAttttt                                                 <  1:3678928/148‑1 (MQ=255)

                                                                                                   ctactaAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCTTAATTTTTc                                                >  2:841160/1‑148 (MQ=255)

                                                                                                    tactaAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCTATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCTTAATTTTTCg                                               >  2:876769/1‑148 (MQ=255)

                                                                                                    tactaAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCTTAATTTTTCg                                               <  2:2998444/148‑1 (MQ=255)

                                                                                                    tactaAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCTTAATTTTTCg                                               <  2:1551507/148‑1 (MQ=255)

                                                                                                          aaaTATTTTATAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCTTAATTTTTCGCTAAAt                                         >  1:720387/1‑148 (MQ=255)

                                                                                                           aaTATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCTATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCTTAATTTTTCGCTAAATc                                        >  1:3355214/1‑148 (MQ=255)

                                                                                                           aaTATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCTTAATTTTTCGCTAAATc                                        <  2:2086573/148‑1 (MQ=255)

                                                                                                             tatTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCTTAATTTTTCGCTAAATCAg                                      >  2:2585059/1‑148 (MQ=255)

                                                                                                              atTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCTATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGTACGGTTAAACATGCCACAATACCCGTATTGAATGCTTAATTTTTCGCTAAATCAgg                                     >  2:2429101/1‑148 (MQ=255)

                                                                                                                tttACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCTTAATTTTTCGCTAAATCAGGat                                   >  2:2732685/1‑148 (MQ=255)

                                                                                                                tttACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCTTAATTTTTCGCTAAATCAGGat                                   >  2:1278262/1‑148 (MQ=255)

                                                                                                                    cAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCTTAATTTTTCGCTAAATCAGGATATTa                               >  2:2387575/1‑148 (MQ=255)

                                                                                                                      aaaTTTCAAATTTAATTGAAAGCTATGGCTATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCTTAATTTTTCGCTAAATCAGGATATTAAc                             <  1:1976747/148‑1 (MQ=255)

                                                                                                                        aTTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCAACAATACCCGTATTGAATGCTTAATTTTTCTCTAAATCATGATATTAACTa                           >  1:3310643/1‑148 (MQ=255)

                                                                                                                          ttCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCTTAATTTTTCGCTAAATCAGGATATTAACTAcc                         <  1:3080236/148‑1 (MQ=255)

                                                                                                                               aTTTAATTGAAAGCTATGGCTATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCTTAATTTTTCGCTAAATCAGGATATTAACTACCCATGc                    >  2:2219675/1‑148 (MQ=255)

                                                                                                                               aTTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCTTAATTTTTCGCTAAAGAAGGATATTAACTAACCATGc                    >  2:2058034/1‑148 (MQ=255)

                                                                                                                                 ttAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCTTAATTTTTCGCTAAATCAGGATATTAACTACCCATGCTg                  <  2:509266/148‑1 (MQ=255)

                                                                                                                                     ttGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCTTAATTTTTCGCTAAATCAGGATATTAACTACCCATGCTGGGTa              >  2:1452559/1‑148 (MQ=255)

                                                                                                                                      tGATAGCTATGGCTATATTGAAAAAGTCATCAACAACTATGATTAGTGTAGGCGCAACATTCAACTGAAAGGTTAAACTTGCAACAATACCCGTATTGAATGCTTAATTTTTCGCTAAATCAGGATATTAACTACCCATGCTGGGTaa             <  1:3199600/148‑1 (MQ=255)

                                                                                                                                        aaaGCTATGGCTATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCTTAATTTTTCGCTAAATCAGGATATTAACTACCCATGCTGGGTAAat           <  1:3465285/148‑1 (MQ=255)

                                                                                                                                        aaaGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCTTAATTTTTCGCTAAATCAGGATATTAACTACCCATGCTGGGTAAat           <  1:2245326/148‑1 (MQ=255)

                                                                                                                                         aaGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCTTAATTTTTCGCTAAATCAGGATATTAACTACCCATGCTGGGTAAata          <  1:233244/148‑1 (MQ=255)

                                                                                                                                            cTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCTTAATTTTTCGCTAAATCAGGATATTAACTACCCATGCTGGGTAAATATaa       >  2:3585169/1‑148 (MQ=255)

                                                                                                                                            cTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCTTAATTTTTCGCTAAATCAGGATATTAACTACCCATGCTGGGTAAATATaa       >  1:1249851/1‑148 (MQ=255)

                                                                                                                                            cTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCTTAATTTTTCGCTAAATCAGGATATTAACTACCCATGCTGGGTAAATATaa       >  1:2547263/1‑148 (MQ=255)

                                                                                                                                             tATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCTTAATTTTTCGCTAAATCAGGATATTAACTACCCATGCTGGGTAAATATaaa      <  2:1658717/148‑1 (MQ=255)

                                                                                                                                              aTGGCTATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCTTAATTTTTCGCTAAATCAGGATATTAACTACCCATGCTGGGTAAATATAAAg     >  2:178092/1‑148 (MQ=255)

                                                                                                                                               tGGCTATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCTTAATTTTTCGCTAAATCAGGATATTAACTACCCATGCTGGGTAAATATAAAGc    <  1:3436748/148‑1 (MQ=255)

                                                                                                                                               tGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCTTAATTTTTCGCTAAATCAGGATATTAACTACCCATGCTGGGTAAATATAAAGc    >  1:148512/1‑148 (MQ=255)

                                                                                                                                               tGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCTTAATTTTTCGCTAAATCAGGATATTAACTACCCATGCTGGGTAAATATAAAGc    <  1:3757007/148‑1 (MQ=255)

                                                                                                                                               tGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCTTAATTTTTCGCTAAATCAGGATATTAACTACCCATGCTGGGTAAATATAAAGc    >  1:2675253/1‑148 (MQ=255)

                                                                                                                                               tGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCTTAATTTTTCGCTAAATCAGGATATTAACTACCCATGCTGGGTAAATATAAAGc    <  2:886775/148‑1 (MQ=255)

                                                                                                                                                 gCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCTTAATTTTTCGCTAAATCAGGATATTAACTACCCATGCTGGGTAAATATAAAGCCg  <  1:1224383/148‑1 (MQ=255)

ACTCGTTCACCTGTTGCAGGTACTTCTTGTCGTACTGTTTTGTGCTATAAACGGCGAGTTTCATAAGACTTTCTCCAGTGATGTTGAATCACATTTAAGCTACTAAAAATATTTTACAAAATTTCAAATTTAATTGAAAGCTATGGCGATATTGAAAAATTCATCAACAACTATGCTTAGTGTAGGCGCAACCTTCAACTGAACGGTTAAACATGCCACAATACCCGTATTGAATGCTTAATTTTTCGCTAAATCAGGATATTAACTACCCATGCTGGGTAAATATAAAGCCG  >  CP009273/1437037‑1437329

Alignment Legend
Aligned base mismatch/match (shaded by quality score): `ATCG/ATCG` `< 3 ≤` `ATCG/ATCG` `< 25 ≤` `ATCG/ATCG` `< 37 ≤` `ATCG/ATCG`
Unaligned base: `atcg` Masked matching base: `atcg` Alignment gap: `‑` Deleted base: `‑`

GATK/CNVnator alignment

N/A