Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F55 I0 R1 519 344.2 11360120 95.0% 10792114 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 3,371,372 G→T 33.3% I65I (ATC→ATA)  rpsI ← 30S ribosomal subunit protein S9

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092733,371,3720GT33.3% 530.8 / 308.4 320I65I (ATC→ATA) rpsI30S ribosomal subunit protein S9
Reads supporting (aligned to +/- strand):  ref base G (101/112);  new base T (59/48);  total (160/160)
Fisher's exact test for biased strand distribution p-value = 2.36e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 323 total aligned reads displayed.

TTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACGCAGTTCAGAACGCAGGGACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACGTTGGTTGATTACGATTTTACCGTTGCCCGGTTTGATGAAAACGCGAGCTGC  >  CP009273/3371223‑3371521
                                                                                                                                                     |                                                                                                                                                     
ttCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACGCAGTTCAGAACGCAGGGACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTg                                                                                                                                                       >  2:5004499/1‑150 (MQ=255)
    tACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACGCAGTTCAGAACGCAGGGACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTTATGt                                                                                                                                                   >  1:2221864/1‑150 (MQ=255)
     aCGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACGCAGTTCAGAACGCAGGGACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTTATGTa                                                                                                                                                  >  1:2526717/1‑150 (MQ=255)
      cGTTCACCCAGACGAGCGTCACGAGTAACGAAGCCAGCTGTACGCAGTTCAGAACGCAGGGACTCGTCGTAGTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTAc                                                                                                                                                 <  2:4863586/150‑1 (MQ=255)
       gTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACGCAGTTCAGAACGCAGGGACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACa                                                                                                                                                >  1:2762537/1‑150 (MQ=255)
       gTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACGCAGTTCAGAACGCAGGGACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACa                                                                                                                                                <  2:3573776/150‑1 (MQ=255)
        ttCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACGCAGTTCAGAACGCAGGGACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTTATGTACAg                                                                                                                                               >  2:1878670/1‑150 (MQ=255)
        ttCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACGCAGTTCAGAACGCAGGGACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAg                                                                                                                                               <  2:2819309/150‑1 (MQ=255)
         tCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACGCAGTTCAGAACGCAGGGACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAgg                                                                                                                                              >  2:917413/1‑150 (MQ=255)
         tCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTGACGCAGTTCAGAACGCAGGGACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAgg                                                                                                                                              <  2:3517947/150‑1 (MQ=255)
          cAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACGCAGTTCAGAACGCAGGGACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTTATGTACAGGt                                                                                                                                             >  2:4760677/1‑150 (MQ=255)
           aaCCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACGCAGTTCAGAACGCAGGGACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTc                                                                                                                                            >  2:1635576/1‑150 (MQ=255)
           aaCCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACGCAGTTCAGAACGCAGGGACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTGTAACGGTGATGTACAGGTc                                                                                                                                            >  2:2381632/1‑150 (MQ=255)
             ccTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACGCAGTTCAGAACGCAGGGACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTCCa                                                                                                                                          <  1:1737378/150‑1 (MQ=255)
             ccTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACGCAGTTCAGAACGCAGGGACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTCCa                                                                                                                                          <  2:4208870/150‑1 (MQ=255)
              cTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACGCAGTTCAGAACGCAGGGACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTCCAg                                                                                                                                         >  2:2244952/1‑150 (MQ=255)
               tGACGAGCGTCACGAGTAACGAAGCCAGCTTTACGCAGTTCAGAACGCAGGGACTCGTCGTATTCCATCAGAGCGCGGTTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCATTAACGGTGATGTACAGGTCCCGt                                                                                                                                        >  2:4406654/1‑150 (MQ=255)
                gACGAGCGTCACGAGTAACGAAGCCAGCTTTACGCAGTTCAGAACGCAGGGACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTTATGTACAGGTCCAGtt                                                                                                                                       <  1:149890/150‑1 (MQ=255)
                   gAGCGTCACGAGTAACGAAGCCAGCTTTACGCAGTTCAGAACGCAGGGACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTTATGTACAGGTCCAGTTtct                                                                                                                                    <  2:1796661/150‑1 (MQ=255)
                     gCGTCACGAGTAACGAAGCCAGCTTTACGCAGTTCAGAACGCAGGGACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTTATGTACAGGTCCAGTTTCTCa                                                                                                                                  <  2:1448933/150‑1 (MQ=255)
                     gCGTCACGAGTAACGAAGCCAGCTTTACGCAGTTCAGAACGCAGGGACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTCCAGTTTCTCa                                                                                                                                  <  2:3232090/150‑1 (MQ=255)
                     gCGTCACGAGTAACGAAGCCAGCTTTACGCAGTTCAGAACGCAGGGACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTCCAGTTTCTCa                                                                                                                                  >  1:5383604/1‑150 (MQ=255)
                      cGTCACGAGTAACGAAGCCAGCTTTACGCAGTTCAGAACGCAGGGACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTCCAGTTTCTCaa                                                                                                                                 <  2:4609337/150‑1 (MQ=255)
                       gTCACGAGTAACGAAGCCAGCTTTACGCAGTTAAGAACGCAGGGACTCGTCGTATTCCATAAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCAGACCAGAGATACCACCACCTTTAACGGATATGTACAGGTCCAGTTTATCAAc                                                                                                                                >  2:4453787/1‑150 (MQ=255)
                        tCACGAGTAACGAAGCCAGCTTTACGCAGTTCAGAACGCAGGGACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTTATGTACAGGTCCAGTTTCTCAAcc                                                                                                                               <  1:2565661/150‑1 (MQ=255)
                          aCGAGTAACGAAGCCAGCTTTACGCAGTTCAGAACGCAGGGACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTTATGTACAGGTCCAGTTTCTCAACCAt                                                                                                                             <  1:5655176/150‑1 (MQ=255)
                           cGAGTAACGAAGCCAGCTTTACGCAGTTCAGAACGCAGGGACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTTATGTACAGGTCCAGTTTCTCAACCATg                                                                                                                            >  1:4536918/1‑150 (MQ=255)
                           cGAGTAACGAAGCCAGCTTTACGCAGTTCAGAACGCAGGGACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTCCAGTTTCTCAACCATg                                                                                                                            >  1:2024953/1‑150 (MQ=255)
                             aGTAACGAAGCCAGCTTTACGCAGTTCAGAACGCAGGGACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTc                                                                                                                          <  1:1701667/150‑1 (MQ=255)
                              gTAACGAAGCCAGCTTTACGCAGTTCAGAACGCAGGGACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCg                                                                                                                         <  1:4040410/150‑1 (MQ=255)
                                   gAAGCCAGCTTTACGCAGTTCAGAACGCAGGGACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAg                                                                                                                    <  1:8455/150‑1 (MQ=255)
                                    aaGCCAGCTTTACGCAGTTCAGAACGCAGGGACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTTATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGt                                                                                                                   >  1:4832862/1‑150 (MQ=255)
                                    aaGCCAGCTTTACGCAGTTCAGAACGCAGGGACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGt                                                                                                                   <  2:2285032/150‑1 (MQ=255)
                                       ccAGCTTTACGCAGTTCAGAACGCAGGGACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTTATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTcc                                                                                                                >  1:1956770/1‑150 (MQ=255)
                                       ccAGCTTTACGCAGTTCAGAACGCAGGGACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTcc                                                                                                                >  1:1410204/1‑150 (MQ=255)
                                           cTTTACGCAGTTCAGAACGCAGGGACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCg                                                                                                            >  2:1891963/1‑150 (MQ=255)
                                            tttACGCAGTTCAGAACGCAGGGACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCgg                                                                                                           >  2:2601569/1‑150 (MQ=255)
                                             ttACGCAGTTCAGAACGCAGGGACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTTATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGc                                                                                                          >  2:1233013/1‑150 (MQ=255)
                                              tACGCAGTTCAGAACGCAGGGACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCt                                                                                                         <  1:2951849/150‑1 (MQ=255)
                                                cGCAGTTCAGAACGCAGGGACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGa                                                                                                       >  1:803678/1‑150 (MQ=255)
                                                  cAGTTCAGTACGCAGGGACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACg                                                                                                     >  2:881798/1‑150 (MQ=255)
                                                   aGTTCAGAACGCAGGGACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGa                                                                                                    >  1:515720/1‑150 (MQ=255)
                                                      tCAGAACGCAGGGACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTTATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACt                                                                                                 >  1:3804173/1‑150 (MQ=255)
                                                            cGCAGGGACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTTATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATg                                                                                           <  2:2703018/150‑1 (MQ=255)
                                                               aGGGACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTTATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCgg                                                                                        >  1:654300/1‑150 (MQ=255)
                                                                gggACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCggg                                                                                       >  1:2040952/1‑150 (MQ=255)
                                                                 ggACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTTATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGc                                                                                      >  2:4917849/1‑150 (MQ=255)
                                                                    cTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGt                                                                                   <  1:1635576/150‑1 (MQ=255)
                                                                     tcgtcgTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTTATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGtt                                                                                  <  1:4291412/150‑1 (MQ=255)
                                                                       gtcgtATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTc                                                                                <  2:4339573/150‑1 (MQ=255)
                                                                        tcgtATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCa                                                                               >  2:955594/1‑150 (MQ=255)
                                                                          gtATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTTATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACg                                                                             <  1:977039/150‑1 (MQ=255)
                                                                            aTTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGAc                                                                           >  1:4922355/1‑150 (MQ=255)
                                                                             ttCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTTATGTACAGGTCCAGTTTCTCAACAATGACGACAAGTTCAAGCGGCTGACTAACTACCATGCGGGCAGTTACACGAcc                                                                          >  2:2882566/1‑150 (MQ=255)
                                                                             ttCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGAcc                                                                          <  1:4435189/150‑1 (MQ=255)
                                                                                 aTCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTTATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAg                                                                      <  2:590065/150‑1 (MQ=255)
                                                                                 aTCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAg                                                                      >  2:4058063/1‑150 (MQ=255)
                                                                                   cAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCTGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTa                                                                    <  1:2652054/150‑1 (MQ=255)
                                                                                     gagCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTTATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACt                                                                  <  2:2142866/150‑1 (MQ=255)
                                                                                      agCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTg                                                                 >  1:4291626/1‑150 (MQ=255)
                                                                                              tGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTTATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCagag                                                         <  2:2468003/150‑1 (MQ=255)
                                                                                              tGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTTATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCagag                                                         <  2:195864/150‑1 (MQ=255)
                                                                                              tGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCagag                                                         <  1:917413/150‑1 (MQ=255)
                                                                                              agATACCGTGACGGCTCGCACCAGCCAGACAAGAGATACCACCACCTATAACGGTTATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCagag                                                         <  2:3059813/149‑1 (MQ=255)
                                                                                               gATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTTATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAgaga                                                        >  1:3068387/1‑150 (MQ=255)
                                                                                                aTACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGaa                                                       >  1:389113/1‑150 (MQ=255)
                                                                                                 tACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAAc                                                      <  1:4975312/150‑1 (MQ=255)
                                                                                                    cGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACgtt                                                   <  1:3686500/150‑1 (MQ=255)
                                                                                                    cGTGACGGATCGCACCAGCCTGACCAGAGAGACCACCACCTTTAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACgtt                                                   <  2:3130791/150‑1 (MQ=255)
                                                                                                     gTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTTATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACgttg                                                  <  1:1451606/150‑1 (MQ=255)
                                                                                                     gTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTTATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACgttg                                                  <  1:1878670/150‑1 (MQ=255)
                                                                                                     gTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACgttg                                                  <  2:2685417/150‑1 (MQ=255)
                                                                                                      tGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACgttgg                                                 >  1:127151/1‑150 (MQ=255)
                                                                                                        aCGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACgttggtt                                               <  1:1092278/150‑1 (MQ=255)
                                                                                                         cGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACggtggttg                                              >  2:3859701/1‑150 (MQ=255)
                                                                                                           gATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACGTTGGTTGAt                                            >  1:1298033/1‑150 (MQ=255)
                                                                                                             tCGCTCCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACGTTGGTTGATTa                                          >  1:5498969/1‑150 (MQ=255)
                                                                                                             tCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACGTTGGTTGATTa                                          <  2:4575188/150‑1 (MQ=255)
                                                                                                               gCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACGTTGGTTGATTACg                                        <  1:5031625/150‑1 (MQ=255)
                                                                                                               gCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACGTTGGTTGATTACg                                        >  1:5253704/1‑150 (MQ=255)
                                                                                                                   cAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACGTTGGTTGATTACGAttt                                    <  2:4890093/150‑1 (MQ=255)
                                                                                                                        tGACCAGAGATACCACCACCTTTAACGGTTATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACGTTGGTTGATTACGATTTTACCg                               >  1:3245069/1‑150 (MQ=255)
                                                                                                                         gACCAGAGATACCACCACCTTTAACGGTTATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACGTGGGTTGATTACGATTTTACCGt                              >  1:1764784/1‑150 (MQ=255)
                                                                                                                         gACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACGTTGGTTGATTACGATTTTACCGt                              <  2:562017/150‑1 (MQ=255)
                                                                                                                                   accaccaccTTTAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACGTTGGTTGATTACGATTTTACCGTTGCCCGGttt                    <  1:4721658/150‑1 (MQ=255)
                                                                                                                                    ccaccaccTTTAACGGTTATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACGTTGGTTGATTACGATTTTACCGTTGCCCGGTTtg                   <  1:1021674/150‑1 (MQ=255)
                                                                                                                                      accaccTTTAACGGTTATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACGTTGGTTGATTACGATTTTACCGTTGCCCGGTTtgat                 >  2:3086497/1‑150 (MQ=255)
                                                                                                                                      accaccTTTAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACGTTGGTTGATTACGATTTTACCGTTGCACGGTTtgat                 >  1:3587083/1‑150 (MQ=255)
                                                                                                                                        caccTTTAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACGTTGGTTGATTACGATTTTACCGTTGCCCGGTTtgatga               <  1:4611756/150‑1 (MQ=255)
                                                                                                                                         accTTTAACGGTTATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACGTTGGTTGATTACGATTTTACCGTTGCCCGGTTTGATGaa              >  1:1545366/1‑150 (MQ=255)
                                                                                                                                          ccTTTAACGGTTATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACGTTGGTTGATTACGATTTTACCGTTGCCCGGTTTGATGaaa             <  2:507134/150‑1 (MQ=255)
                                                                                                                                          ccTTTAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACGTTGGTTGATTACGATTTTACCGTTGCCCGGTTTGATGaaa             <  2:151425/150‑1 (MQ=255)
                                                                                                                                            tttAACGGTTATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACGTTGGTTGATTACGATTTTACCGTTGCCCGGTTTGATGAAAAc           >  2:830082/1‑150 (MQ=255)
                                                                                                                                            tttAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACGTTGGTTGATTACGATTTTACCGTTGCCCGGTTTGATGAAAAc           <  1:1181613/150‑1 (MQ=255)
                                                                                                                                             ttAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACGTTGGTTGATTACGATTTTACCGTTGCCCGGTTTGATGAAAAcg          <  2:5380366/150‑1 (MQ=255)
                                                                                                                                             ttAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACGTTGGTTGATTACGATTTTACCGTTGCCCGGTTTGATGAAAAcg          <  2:1425909/150‑1 (MQ=255)
                                                                                                                                              tAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACGTTGGTTGATTACGATTTTACCGTTGCCCGGTTTGATGAAAAcgc         >  2:3766752/1‑150 (MQ=255)
                                                                                                                                              tAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACGTTGGTTGATTACGATTTTACCGTTGCCCGGTTTGATGAAAAcgc         >  1:4666538/1‑150 (MQ=255)
                                                                                                                                                  ggTTATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACGTTGGTTGATTACGATTTTACCGTTGCCCGGTTTGATGAAAACGCGAGc     >  2:5552703/1‑150 (MQ=255)
                                                                                                                                                     gATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACGTTGGTTGATTACGATTTTACCGTTGCCCGGTTTGATGAAAACGCGAGCTGc  >  1:414248/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                     
TTCTTACGTTCAACCTGACGAGCGTCACGAGTAACGAAGCCAGCTTTACGCAGTTCAGAACGCAGGGACTCGTCGTATTCCATCAGAGCGCGGGTGATACCGTGACGGATCGCACCAGCCTGACCAGAGATACCACCACCTTTAACGGTGATGTACAGGTCCAGTTTCTCAACCATGTCGACCAGTTCCAGCGGCTGACGAACTACCATGCGGGCAGTTTCACGACCGAAGTACTGTTCCAGAGAACGTTGGTTGATTACGATTTTACCGTTGCCCGGTTTGATGAAAACGCGAGCTGC  >  CP009273/3371223‑3371521

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A