Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F60 I0 R1 465 320.4 10502068 95.5% 10029474 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,264,143 G→A 78.2% intergenic (+119/‑62) mepS → / → rtn murein DD‑endopeptidase, space‑maker hydrolase, mutational suppressor of prc thermosensitivity, outer membrane lipoprotein/resistance protein for phages lambda and N4, putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,264,1430GA78.2% 477.3 / 142.5 223intergenic (+119/‑62)mepS/rtnmurein DD‑endopeptidase, space‑maker hydrolase, mutational suppressor of prc thermosensitivity, outer membrane lipoprotein/resistance protein for phages lambda and N4, putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base G (20/28);  new base A (73/101);  total (94/129)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.18e-01

Only 100 of 225 total aligned reads displayed.

GAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATACTGGTGAGT  >  CP009273/2263995‑2264288
                                                                                                                                                    |                                                                                                                                                 
gAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAgg                                                                                                                                                   <  1:4715448/149‑1 (MQ=255)
        ccGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTc                                                                                                                                           >  2:1214295/1‑149 (MQ=255)
          gggTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCg                                                                                                                                         >  2:615182/1‑149 (MQ=255)
          gggTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCg                                                                                                                                         >  2:3563242/1‑149 (MQ=255)
            gTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGt                                                                                                                                       <  1:1662053/149‑1 (MQ=255)
             ttCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTc                                                                                                                                      >  2:1727193/1‑149 (MQ=255)
             ttCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTc                                                                                                                                      <  1:3692576/149‑1 (MQ=255)
               ctcAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCAt                                                                                                                                    <  2:3983085/149‑1 (MQ=255)
                 cAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTg                                                                                                                                  >  1:4542208/1‑149 (MQ=255)
                  aGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGt                                                                                                                                 <  1:1125237/149‑1 (MQ=255)
                  aGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGt                                                                                                                                 <  1:4595356/149‑1 (MQ=255)
                  aGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGt                                                                                                                                 <  1:2871947/149‑1 (MQ=255)
                    ccGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTa                                                                                                                               <  1:1935868/149‑1 (MQ=255)
                       cAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCt                                                                                                                            <  1:4081297/149‑1 (MQ=255)
                         gCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCttt                                                                                                                          <  2:2007939/149‑1 (MQ=255)
                         gCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCttt                                                                                                                          <  1:5170038/149‑1 (MQ=255)
                              tacaCCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAAt                                                                                                                     <  1:4855351/146‑1 (MQ=255)
                               aaaCCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAAtg                                                                                                                    <  2:5081043/149‑1 (MQ=255)
                                aaCCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAAtgt                                                                                                                   <  1:2345832/149‑1 (MQ=255)
                                 aCCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAAtgtt                                                                                                                  >  1:4591621/1‑149 (MQ=255)
                                 aCCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAAtgtt                                                                                                                  <  1:476461/149‑1 (MQ=255)
                                 aCCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAAtgtt                                                                                                                  <  2:364593/149‑1 (MQ=255)
                                  ccGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAAtgttg                                                                                                                 >  1:941151/1‑149 (MQ=255)
                                  ccGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCAGTGTTATCTTTTAAAtgttg                                                                                                                 >  2:1972443/1‑149 (MQ=255)
                                    gTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTc                                                                                                               <  1:1805943/149‑1 (MQ=255)
                                    gTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTc                                                                                                               >  2:4848776/1‑149 (MQ=255)
                                       tGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTa                                                                                                            <  2:3810627/149‑1 (MQ=255)
                                         gATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAAt                                                                                                          <  1:4945472/149‑1 (MQ=255)
                                         gATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAAt                                                                                                          <  2:3936800/149‑1 (MQ=255)
                                          aTGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAAtt                                                                                                         <  2:2627095/149‑1 (MQ=255)
                                              actCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAg                                                                                                     <  1:1392741/147‑1 (MQ=255)
                                              aaTCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAg                                                                                                     <  2:3043341/149‑1 (MQ=255)
                                               aTCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAgg                                                                                                    >  2:2414977/1‑149 (MQ=255)
                                               aTCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGATTAGGCAGAGATTAATTGTTTTAATTAATCAGAGAAAATGATGTTTCAGAGTCTAGATCCAGTCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGAATTAATTTAAgt                                                                                                    >  2:645720/1‑148 (MQ=255)
                                                    ttGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAAtt                                                                                               <  2:3244540/149‑1 (MQ=255)
                                                    ttGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAAtt                                                                                               <  2:755646/149‑1 (MQ=255)
                                                    ttGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAAtt                                                                                               <  1:268792/149‑1 (MQ=255)
                                                    ttGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAAtt                                                                                               <  2:4156882/149‑1 (MQ=255)
                                                      ggCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTGCGGTCATTGTTATCTTTTAAATGTTTTCGTAATTTCAGGAAATTaa                                                                                             >  2:262031/1‑149 (MQ=255)
                                                      ggCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTaa                                                                                             <  2:3843371/149‑1 (MQ=255)
                                                           tCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGaa                                                                                        >  1:5178081/1‑149 (MQ=255)
                                                            ccTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAAt                                                                                       >  2:3041813/1‑149 (MQ=255)
                                                             cTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATc                                                                                      <  1:747279/149‑1 (MQ=255)
                                                              tGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCa                                                                                     <  1:601311/149‑1 (MQ=255)
                                                              tGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCa                                                                                     <  2:2724471/149‑1 (MQ=255)
                                                                aCGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATg                                                                                   <  1:4261550/149‑1 (MQ=255)
                                                                aCGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATg                                                                                   <  1:1966488/149‑1 (MQ=255)
                                                                 cGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGt                                                                                  <  2:4591621/149‑1 (MQ=255)
                                                                    gTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCa                                                                               >  1:1527832/1‑149 (MQ=255)
                                                                      tAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATa                                                                             >  2:2356429/1‑149 (MQ=255)
                                                                           gAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACgcgc                                                                        <  2:4557751/149‑1 (MQ=255)
                                                                            aaaGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCt                                                                       >  1:3362744/1‑149 (MQ=255)
                                                                                cACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTAGAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCa                                                                   <  2:4807761/149‑1 (MQ=255)
                                                                                 aCTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCaa                                                                  <  1:4157558/149‑1 (MQ=255)
                                                                                     cTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAAtttt                                                              >  1:5032359/1‑149 (MQ=255)
                                                                                      ttAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTg                                                             <  1:4187957/149‑1 (MQ=255)
                                                                                       tAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTgg                                                            >  1:121499/1‑149 (MQ=255)
                                                                                         ggCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATATTCCGGTCATTGTTATCTTTTAAATTTTGTCGTAATTTCAGGAACTTAAAGGAATCATGTTCATACGCGCTCCCAATTTTGGAc                                                          >  1:2653985/1‑149 (MQ=255)
                                                                                            aGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTa                                                       >  1:967446/1‑149 (MQ=255)
                                                                                              tGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGACTCATGTTCATACGCGCTCCCAATTTTGGACGTAAg                                                     >  2:2941673/1‑149 (MQ=255)
                                                                                                cTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATGTTGGACGTAAGCt                                                   >  2:3398627/1‑149 (MQ=255)
                                                                                                     ttGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGc                                              >  1:3506895/1‑149 (MQ=255)
                                                                                                     ttGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGc                                              >  1:817832/1‑149 (MQ=255)
                                                                                                     ttGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGc                                              >  1:2862523/1‑149 (MQ=255)
                                                                                                      tGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCt                                             <  1:167621/149‑1 (MQ=255)
                                                                                                       gTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCtt                                            <  2:3666323/149‑1 (MQ=255)
                                                                                                           tcattcatCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCt                                        <  2:420012/149‑1 (MQ=255)
                                                                                                            cattcatCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTg                                       >  1:3112939/1‑149 (MQ=255)
                                                                                                             attcatCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGc                                      >  1:564876/1‑149 (MQ=255)
                                                                                                              ttcatCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCa                                     >  2:153132/1‑149 (MQ=255)
                                                                                                               tcatcaGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCAt                                    <  2:2660627/149‑1 (MQ=255)
                                                                                                                  tcaGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCAttgt                                 >  1:3057578/1‑149 (MQ=255)
                                                                                                                   caGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCAttgtt                                <  1:655913/149‑1 (MQ=255)
                                                                                                                   caGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCAttgtt                                <  2:4325391/149‑1 (MQ=255)
                                                                                                                   caGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCAttgtt                                <  2:4025065/149‑1 (MQ=255)
                                                                                                                   caGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCAttgtt                                <  1:1258686/149‑1 (MQ=255)
                                                                                                                     gagaAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCACTTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGc                              <  2:2241588/149‑1 (MQ=255)
                                                                                                                     gagaAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGc                              <  1:3597329/149‑1 (MQ=255)
                                                                                                                      agaAAATGATGTTTCCGCGTCATGATCCAGACTATATTCCGGTCATTGTTATCTTTTAAATGTAGTCGTAATTAAAGGAAATTAACGGAATCATGTTCATACGCGCTCACAATTTTGGACGTAAGCTCCTGCTTACCTGCAtagatgaa                             >  1:3307648/1‑142 (MQ=255)
                                                                                                                       gaAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAg                            <  1:2296901/149‑1 (MQ=255)
                                                                                                                        aaaaTGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAgg                           >  1:4795552/1‑149 (MQ=255)
                                                                                                                           atgatgTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGt                        <  1:3644958/149‑1 (MQ=255)
                                                                                                                           atgatgTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGt                        >  2:181238/1‑149 (MQ=255)
                                                                                                                            tgatgTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTa                       >  1:668865/1‑149 (MQ=255)
                                                                                                                            tgatgTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTa                       >  2:4598824/1‑149 (MQ=255)
                                                                                                                             gatgTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTaa                      <  2:246454/149‑1 (MQ=255)
                                                                                                                             gatgTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTaa                      >  2:4449560/1‑149 (MQ=255)
                                                                                                                             gatgTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTaa                      >  2:2520089/1‑149 (MQ=255)
                                                                                                                                gTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGa                   >  1:4503098/1‑149 (MQ=255)
                                                                                                                                gTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGa                   >  2:3227979/1‑149 (MQ=255)
                                                                                                                                      gcgTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGa             >  2:4269844/1‑149 (MQ=255)
                                                                                                                                        gTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATa           >  2:4649154/1‑149 (MQ=255)
                                                                                                                                         tCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATAc          >  2:804153/1‑149 (MQ=255)
                                                                                                                                         tCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATAc          <  2:2326906/149‑1 (MQ=255)
                                                                                                                                         tCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATAc          >  1:2162701/1‑149 (MQ=255)
                                                                                                                                         tCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATAc          >  2:5155527/1‑149 (MQ=255)
                                                                                                                                         tCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATAc          >  1:1700713/1‑149 (MQ=255)
                                                                                                                                               tCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATACTGGTGa    >  1:1919458/1‑149 (MQ=255)
                                                                                                                                               tCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATACTGGTGa    <  2:1685999/149‑1 (MQ=255)
                                                                                                                                                 cAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATCTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATACTGGTGAGt  <  1:2433145/149‑1 (MQ=255)
                                                                                                                                                    |                                                                                                                                                 
GAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATACTGGTGAGT  >  CP009273/2263995‑2264288

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATACTGGTGAGTTGCC  >  CP009273/2263995‑2264292
                                                                                                                                                    |                                                                                                                                                     
GAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGC                                                                                                                                                      <  SRR19973084.4734524/150‑1 (MQ=60)
GAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGC                                                                                                                                                      <  SRR19973084.4734544/150‑1 (MQ=60)
 AAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACT                                                                                                                                                     <  SRR19973084.4053314/150‑1 (MQ=60)
 AAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACT                                                                                                                                                     <  SRR19973084.1931208/150‑1 (MQ=60)
      CGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACAATAGT                                                                                                                                                >  SRR19973084.4620069/1‑150 (MQ=60)
       GCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTC                                                                                                                                               <  SRR19973084.5243586/150‑1 (MQ=60)
            GTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTC                                                                                                                                          <  SRR19973084.3522465/150‑1 (MQ=60)
            GTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTC                                                                                                                                          <  SRR19973084.1669009/150‑1 (MQ=60)
            GTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGGCCGGTC                                                                                                                                          >  SRR19973084.3635670/1‑150 (MQ=60)
             TTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCA                                                                                                                                         <  SRR19973084.3707679/150‑1 (MQ=60)
               CTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATT                                                                                                                                       <  SRR19973084.742012/150‑1 (MQ=60)
                TCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTG                                                                                                                                      >  SRR19973084.4560785/1‑150 (MQ=60)
                  AGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTT                                                                                                                                    <  SRR19973084.1130041/150‑1 (MQ=60)
                  AGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTT                                                                                                                                    <  SRR19973084.4614129/150‑1 (MQ=60)
                  AGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTT                                                                                                                                    <  SRR19973084.2883831/150‑1 (MQ=60)
                  AGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTT                                                                                                                                    <  SRR19973084.2883826/150‑1 (MQ=60)
                    CCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTAT                                                                                                                                  <  SRR19973084.3701410/150‑1 (MQ=60)
                    CCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTAT                                                                                                                                  <  SRR19973084.1943847/150‑1 (MQ=60)
                    CCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTAT                                                                                                                                  <  SRR19973084.1944129/150‑1 (MQ=60)
                     CGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATC                                                                                                                                 <  SRR19973084.1251300/150‑1 (MQ=60)
                      GCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCT                                                                                                                                >  SRR19973084.4292572/1‑150 (MQ=60)
                       CAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTT                                                                                                                               >  SRR19973084.1676195/1‑150 (MQ=60)
                       CAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTT                                                                                                                               <  SRR19973084.4097915/150‑1 (MQ=60)
                         GCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTT                                                                                                                             <  SRR19973084.5190922/150‑1 (MQ=60)
                              TACACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATG                                                                                                                        <  SRR19973084.4874922/150‑1 (MQ=60)
                               AAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGT                                                                                                                       <  SRR19973084.960090/150‑1 (MQ=60)
                               AAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGT                                                                                                                       >  SRR19973084.3368018/1‑150 (MQ=60)
                                AACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTT                                                                                                                      <  SRR19973084.2355719/150‑1 (MQ=60)
                                AACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTT                                                                                                                      >  SRR19973084.4610568/1‑150 (MQ=60)
                                AACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTT                                                                                                                      >  SRR19973084.1984241/1‑150 (MQ=60)
                                AACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTT                                                                                                                      >  SRR19973084.4610384/1‑150 (MQ=60)
                                AACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTT                                                                                                                      <  SRR19973084.648620/150‑1 (MQ=60)
                                 ACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTG                                                                                                                     <  SRR19973084.478441/150‑1 (MQ=60)
                                 ACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTG                                                                                                                     >  SRR19973084.945129/1‑150 (MQ=60)
                                  CCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGT                                                                                                                    >  SRR19973084.1361318/1‑150 (MQ=60)
                                   AGTTTGGAAGCAATCCCTTGTCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTTCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTC                                                                                                                   <  SRR19973084.2841679/150‑1 (MQ=60)
                                    GTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCG                                                                                                                  <  SRR19973084.1813507/150‑1 (MQ=60)
                                        GGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAAT                                                                                                              <  SRR19973084.4251966/150‑1 (MQ=60)
                                         GATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATT                                                                                                             <  SRR19973084.4965542/150‑1 (MQ=60)
                                           TGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTC                                                                                                           <  SRR19973084.1158855/150‑1 (MQ=60)
                                             CAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAG                                                                                                         <  SRR19973084.2342394/150‑1 (MQ=60)
                                              ACTCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGG                                                                                                        <  SRR19973084.1398564/150‑1 (MQ=60)
                                                   CTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATT                                                                                                   <  SRR19973084.4594289/150‑1 (MQ=60)
                                                    TTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTA                                                                                                  <  SRR19973084.270019/150‑1 (MQ=60)
                                                    TTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTA                                                                                                  <  SRR19973084.269324/150‑1 (MQ=60)
                                                        CTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGG                                                                                              >  SRR19973084.801631/1‑150 (MQ=60)
                                                         TATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGA                                                                                             >  SRR19973084.180090/1‑150 (MQ=60)
                                                         TATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGA                                                                                             >  SRR19973084.180096/1‑150 (MQ=60)
                                                         TATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGA                                                                                             >  SRR19973084.4156501/1‑150 (MQ=60)
                                                         TATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGA                                                                                             >  SRR19973084.1529629/1‑150 (MQ=60)
                                                          ATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAA                                                                                            >  SRR19973084.5198778/1‑150 (MQ=60)
                                                          ATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAA                                                                                            >  SRR19973084.5198981/1‑150 (MQ=60)
                                                             CTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCA                                                                                         <  SRR19973084.750509/150‑1 (MQ=60)
                                                              TGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCAT                                                                                        <  SRR19973084.3237902/150‑1 (MQ=60)
                                                              TGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCAT                                                                                        <  SRR19973084.604031/150‑1 (MQ=60)
                                                               GACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATG                                                                                       <  SRR19973084.4901398/150‑1 (MQ=60)
                                                                ACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGT                                                                                      <  SRR19973084.1974875/150‑1 (MQ=60)
                                                                ACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGT                                                                                      <  SRR19973084.4278877/150‑1 (MQ=60)
                                                                ACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGT                                                                                      <  SRR19973084.1974902/150‑1 (MQ=60)
                                                                ACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGGCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGT                                                                                      <  SRR19973084.1974874/150‑1 (MQ=60)
                                                                  GAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTC                                                                                    <  SRR19973084.1466426/150‑1 (MQ=60)
                                                                   AGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCA                                                                                   >  SRR19973084.1534330/1‑150 (MQ=60)
                                                                         CTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCG                                                                             <  SRR19973084.4749181/150‑1 (MQ=60)
                                                                           GAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCT                                                                           >  SRR19973084.3376703/1‑150 (MQ=60)
                                                                           GAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCT                                                                           >  SRR19973084.2979549/1‑150 (MQ=60)
                                                                           GAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCT                                                                           >  SRR19973084.3376525/1‑150 (MQ=60)
                                                                               GCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCA                                                                       >  SRR19973084.2388488/1‑150 (MQ=60)
                                                                                CACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAA                                                                      >  SRR19973084.3431971/1‑150 (MQ=60)
                                                                                 ACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAAT                                                                     <  SRR19973084.4174508/150‑1 (MQ=60)
                                                                                 ACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAAT                                                                     <  SRR19973084.4174773/150‑1 (MQ=60)
                                                                                    GCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTT                                                                  >  SRR19973084.5052832/1‑150 (MQ=60)
                                                                                     CTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTG                                                                 >  SRR19973084.4837698/1‑150 (MQ=60)
                                                                                      TTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGG                                                                <  SRR19973084.4205020/150‑1 (MQ=60)
                                                                                      TTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGG                                                                >  SRR19973084.1569572/1‑150 (MQ=60)
                                                                                      TTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGG                                                                >  SRR19973084.122219/1‑150 (MQ=60)
                                                                                        AGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATATTCCGGTCATTGTTATCTTTTAAATTTTGTCGTAATTTCAGGAACTTAAAGGAATCATGTTCATACGCGCTCCCAATTTTGGAC                                                              >  SRR19973084.2664910/1‑150 (MQ=60)
                                                                                        AGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGAC                                                              >  SRR19973084.2241757/1‑150 (MQ=60)
                                                                                           CAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTA                                                           >  SRR19973084.4061943/1‑150 (MQ=60)
                                                                                           CAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTA                                                           >  SRR19973084.971572/1‑150 (MQ=60)
                                                                                            AGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAA                                                          <  SRR19973084.5199269/150‑1 (MQ=60)
                                                                                                   TTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTG                                                   <  SRR19973084.1701492/150‑1 (MQ=60)
                                                                                                    TTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGC                                                  >  SRR19973084.3521014/1‑150 (MQ=60)
                                                                                                    TTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGC                                                  >  SRR19973084.821219/1‑150 (MQ=60)
                                                                                                    TTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGC                                                  >  SRR19973084.2874375/1‑150 (MQ=60)
                                                                                                      TGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTT                                                <  SRR19973084.168525/150‑1 (MQ=60)
                                                                                                         TTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACC                                             <  SRR19973084.713813/150‑1 (MQ=60)
                                                                                                          TTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCT                                            <  SRR19973084.2617638/150‑1 (MQ=60)
                                                                                                           TCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTG                                           >  SRR19973084.3125717/1‑150 (MQ=60)
                                                                                                            CATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGC                                          >  SRR19973084.567414/1‑150 (MQ=60)
                                                                                                                 ATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGT                                     <  SRR19973084.4532014/150‑1 (MQ=60)
                                                                                                                 ATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGT                                     >  SRR19973084.3069948/1‑150 (MQ=60)
                                                                                                                  TCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTT                                    <  SRR19973084.887804/150‑1 (MQ=60)
                                                                                                                   CAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTG                                   <  SRR19973084.659020/150‑1 (MQ=60)
                                                                                                                   CAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTG                                   >  SRR19973084.3750557/1‑150 (MQ=60)
                                                                                                                   CAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTG                                   <  SRR19973084.658835/150‑1 (MQ=60)
                                                                                                                   CAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTG                                   <  SRR19973084.1263842/150‑1 (MQ=60)
                                                                                                                     GAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCA                                 >  SRR19973084.2663209/1‑150 (MQ=60)
                                                                                                                     GAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCA                                 <  SRR19973084.3612048/150‑1 (MQ=60)
                                                                                                                     GAGAAAATGATGTTTCCGCGTCATGATCCAGACTATATTCCGGTCATTGTTATCTTTTAAATGTAGTCGTAATTAAAGGAAATTAACGGAATCATGTTCATACGCGCTCACAATTTTGGACGTAAGCTCCTGCTTACCTGCATagatgaa                                 >  SRR19973084.3321282/1‑143 (MQ=60)
                                                                                                                       GAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGG                               >  SRR19973084.4814807/1‑150 (MQ=60)
                                                                                                                       GAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGG                               <  SRR19973084.2306549/150‑1 (MQ=60)
                                                                                                                          AATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGT                            >  SRR19973084.4471389/1‑150 (MQ=60)
                                                                                                                           ATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTA                           >  SRR19973084.671835/1‑150 (MQ=60)
                                                                                                                           ATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTA                           >  SRR19973084.4972053/1‑150 (MQ=60)
                                                                                                                           ATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTA                           <  SRR19973084.3659883/150‑1 (MQ=60)
                                                                                                                             GATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAAT                         <  SRR19973084.5111277/150‑1 (MQ=60)
                                                                                                                              ATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATG                        >  SRR19973084.28894/1‑150 (MQ=60)
                                                                                                                               TGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGA                       >  SRR19973084.4521484/1‑150 (MQ=60)
                                                                                                                                   TCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGC                   >  SRR19973084.489091/1‑150 (MQ=60)
                                                                                                                                        GTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATAC              >  SRR19973084.2171680/1‑150 (MQ=60)
                                                                                                                                        GTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATAC              >  SRR19973084.4797283/1‑150 (MQ=60)
                                                                                                                                        GTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATAC              >  SRR19973084.1707744/1‑150 (MQ=60)
                                                                                                                                             GATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATACTGGTG         >  SRR19973084.603160/1‑150 (MQ=60)
                                                                                                                                             GATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATACTGGTG         >  SRR19973084.3092452/1‑150 (MQ=60)
                                                                                                                                              ATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATACTGGTGA        >  SRR19973084.1927648/1‑150 (MQ=60)
                                                                                                                                              ATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATACTGGTGA        >  SRR19973084.1927641/1‑150 (MQ=60)
                                                                                                                                                 CAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATCTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATACTGGTGAGTT     <  SRR19973084.2443385/150‑1 (MQ=60)
                                                                                                                                                  AGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATACTGGTGAGTTG    >  SRR19973084.4979629/1‑150 (MQ=60)
                                                                                                                                                  AGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATACTGGTGAGTTG    >  SRR19973084.4979287/1‑150 (MQ=60)
                                                                                                                                                    ACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATACTGGTGAGTTGCC  <  SRR19973084.4660327/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
GAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATACTGGTGAGTTGCC  >  CP009273/2263995‑2264292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑