Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F121 I0 R1 856 273.6 9081716 94.1% 8545894 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,264,143 G→A 87.5% intergenic (+119/‑62) mepS → / → rtn murein DD‑endopeptidase, space‑maker hydrolase, mutational suppressor of prc thermosensitivity, outer membrane lipoprotein/resistance protein for phages lambda and N4, putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,264,1430GA87.5% 706.6 / 78.8 243intergenic (+119/‑62)mepS/rtnmurein DD‑endopeptidase, space‑maker hydrolase, mutational suppressor of prc thermosensitivity, outer membrane lipoprotein/resistance protein for phages lambda and N4, putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base G (15/15);  new base A (83/129);  total (99/144)
Fisher's exact test for biased strand distribution p-value = 3.21e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.13e-01

Only 100 of 244 total aligned reads displayed.

AGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATACTGGTGAGTTGCC  >  CP009273/2263997‑2264292
                                                                                                                                                  |                                                                                                                                                     
aGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGGGTGGATGCAATCCCTGGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGGGCTTGTTTGTTTTCATTCATCAGAGACAATGATGTTTCCGCGTCTTGATCCAGACtc                                                                                                                                                    >  2:2263227/1‑149 (MQ=255)
  cACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACtata                                                                                                                                                  >  1:3969271/1‑150 (MQ=255)
  cACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACtata                                                                                                                                                  >  2:4091363/1‑150 (MQ=255)
   aCGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAg                                                                                                                                                 <  1:2690318/150‑1 (MQ=255)
   aCGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAg                                                                                                                                                 <  2:176575/150‑1 (MQ=255)
      ccGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTcc                                                                                                                                              <  1:2350910/150‑1 (MQ=255)
       cGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCg                                                                                                                                             >  1:1567537/1‑150 (MQ=255)
       cGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCAGTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCg                                                                                                                                             >  2:2704293/1‑150 (MQ=255)
        gggTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCgg                                                                                                                                            >  1:379016/1‑150 (MQ=255)
         ggTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGt                                                                                                                                           <  1:3098634/150‑1 (MQ=255)
         ggTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGt                                                                                                                                           <  2:527175/150‑1 (MQ=255)
         ggTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGAAGTTTCCGCGTCTTGATCCAGACTATAGTCCGGt                                                                                                                                           >  1:2426927/1‑150 (MQ=255)
          gTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTc                                                                                                                                          >  2:3392165/1‑150 (MQ=255)
          gTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTc                                                                                                                                          >  2:3946614/1‑150 (MQ=255)
            tctcAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCAt                                                                                                                                        >  2:2669492/1‑150 (MQ=255)
            tctcAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTCTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCag                                                                                                                                        >  2:4003545/1‑149 (MQ=255)
                aGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGtt                                                                                                                                    <  2:124676/150‑1 (MQ=255)
                   cGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATc                                                                                                                                 >  1:1004936/1‑150 (MQ=255)
                   cGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATc                                                                                                                                 <  2:198069/150‑1 (MQ=255)
                   cGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATc                                                                                                                                 <  2:2464238/150‑1 (MQ=255)
                   cGCAGCTAATAAACCGTTTGGATGCAATCCCGTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTGTTCATTCATCAGAGAAAAGGATGTTTCCGCGTCTTGATCCAGGCGATAGTCCGGTCAGGGTTagc                                                                                                                                 >  2:294305/1‑148 (MQ=255)
                    gCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCt                                                                                                                                <  2:4437450/150‑1 (MQ=255)
                     cAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCtt                                                                                                                               <  2:3213894/150‑1 (MQ=255)
                      agataataaACCGTTTGGCTGCAATCCCTTGGCTATGCTGAAGAGTTAACTGAAAGCACTGCTTAGGCAGTTCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCttt                                                                                                                              <  2:741795/147‑1 (MQ=255)
                        cTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTa                                                                                                                            <  1:2475405/150‑1 (MQ=255)
                          aataaACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTaaa                                                                                                                          >  1:794158/1‑150 (MQ=255)
                           ataaACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAAt                                                                                                                         <  2:4384426/150‑1 (MQ=255)
                            taaACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAAtg                                                                                                                        <  1:4294315/150‑1 (MQ=255)
                             aaaCCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAAtgt                                                                                                                       <  2:2595943/150‑1 (MQ=255)
                               aCCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAAtgttg                                                                                                                     >  1:434381/1‑150 (MQ=255)
                                ccGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAAtgttgt                                                                                                                    <  1:4018574/150‑1 (MQ=255)
                                ccGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAAtgttgt                                                                                                                    >  1:3460088/1‑150 (MQ=255)
                                ccGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAAtgttgt                                                                                                                    <  1:3653857/150‑1 (MQ=255)
                                  gTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCg                                                                                                                  <  2:3495711/150‑1 (MQ=255)
                                  gTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCg                                                                                                                  >  2:4138869/1‑150 (MQ=255)
                                     tGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTaa                                                                                                               >  1:2463162/1‑150 (MQ=255)
                                        aTGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAAttt                                                                                                            <  2:148128/150‑1 (MQ=255)
                                        aTGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAAttt                                                                                                            <  2:1076745/150‑1 (MQ=255)
                                         tGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTc                                                                                                           <  2:1111708/150‑1 (MQ=255)
                                          gCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCa                                                                                                          <  2:702397/150‑1 (MQ=255)
                                          gCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGGGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCGATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCa                                                                                                          >  1:3311351/1‑150 (MQ=255)
                                            aaTCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAgg                                                                                                        <  2:3339651/150‑1 (MQ=255)
                                              tCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGaa                                                                                                      >  1:1112632/1‑150 (MQ=255)
                                                ccTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAAt                                                                                                    >  2:1983934/1‑150 (MQ=255)
                                                 cTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAAtt                                                                                                   <  1:4453580/150‑1 (MQ=255)
                                                  ttGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTa                                                                                                  <  2:3825876/150‑1 (MQ=255)
                                                    gtcTATCCTGACGAGTTAACTGTAAGGACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATATGAGAAAATGATGTTTCTGCGTCTTGATCCAGACTATAGTCCGGTAATTGTTATCTTTTATTTGTTGTCGTAATTTCAGGTAATTAAc                                                                                                <  2:2145350/148‑1 (MQ=255)
                                                    ggCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAAc                                                                                                <  2:3167858/150‑1 (MQ=255)
                                                    ggCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAAc                                                                                                <  2:3574159/150‑1 (MQ=255)
                                                     gCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACg                                                                                               >  1:195573/1‑150 (MQ=255)
                                                      cTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACgg                                                                                              >  1:2001935/1‑150 (MQ=255)
                                                      cTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACgg                                                                                              <  2:1031033/150‑1 (MQ=255)
                                                      cTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACgg                                                                                              >  1:1101223/1‑150 (MQ=255)
                                                               cGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGtt                                                                                     <  1:485882/150‑1 (MQ=255)
                                                               cGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTAAGGAAATTAACGGAATCATGtt                                                                                     >  2:2713408/1‑150 (MQ=255)
                                                                gAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTc                                                                                    <  1:263983/150‑1 (MQ=255)
                                                                    tAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATAc                                                                                <  2:1707281/150‑1 (MQ=255)
                                                                      aCTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATAcgc                                                                              <  2:282974/150‑1 (MQ=255)
                                                                      aCTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATAcgc                                                                              >  1:4284224/1‑150 (MQ=255)
                                                                              cACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCTAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCaa                                                                      <  2:4523246/150‑1 (MQ=255)
                                                                                cTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAAtt                                                                    >  2:3740164/1‑150 (MQ=255)
                                                                                cTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAAtt                                                                    >  2:1456601/1‑150 (MQ=255)
                                                                                cTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAAtt                                                                    <  1:1034255/150‑1 (MQ=255)
                                                                                cTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAAtt                                                                    <  2:363681/150‑1 (MQ=255)
                                                                                 tGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAAttt                                                                   <  1:634123/150‑1 (MQ=255)
                                                                                  gCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAAtttt                                                                  <  2:4535081/150‑1 (MQ=255)
                                                                                       ggCAGTGCTTTTTTGTTTTCATTCATCAGAGAATATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACg                                                             <  1:3495366/150‑1 (MQ=255)
                                                                                         cAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTa                                                           >  1:2825124/1‑150 (MQ=255)
                                                                                            tGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCCATTTTGGACGTAAGc                                                        >  1:773202/1‑150 (MQ=255)
                                                                                            tGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGc                                                        <  2:4202981/150‑1 (MQ=255)
                                                                                            tGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGc                                                        <  2:3433293/150‑1 (MQ=255)
                                                                                               ttttttGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTcc                                                     >  2:622633/1‑150 (MQ=255)
                                                                                               ttttttGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTcc                                                     >  1:3571746/1‑150 (MQ=255)
                                                                                                 ttttGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTg                                                   <  1:324050/150‑1 (MQ=255)
                                                                                                    tGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCtt                                                >  2:2804726/1‑150 (MQ=255)
                                                                                                    tGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCtt                                                >  1:1753063/1‑150 (MQ=255)
                                                                                                    tGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCtt                                                <  1:1875794/150‑1 (MQ=255)
                                                                                                     gTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTa                                               >  2:2796696/1‑150 (MQ=255)
                                                                                                      ttttCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTAc                                              <  1:3944934/150‑1 (MQ=255)
                                                                                                      ttttCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTAc                                              <  1:4091336/150‑1 (MQ=255)
                                                                                                        ttcattcaTCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCt                                            >  2:236904/1‑150 (MQ=255)
                                                                                                             tcatcaGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCAtt                                       <  1:1041413/150‑1 (MQ=255)
                                                                                                              catcaGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCAttg                                      <  2:1600008/150‑1 (MQ=255)
                                                                                                                 caGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCAttgttg                                   <  2:3799885/150‑1 (MQ=255)
                                                                                                                  agagAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGc                                  >  2:3186071/1‑150 (MQ=255)
                                                                                                                     gaAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAgg                               <  2:3704669/150‑1 (MQ=255)
                                                                                                                        aaTGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGt                            >  2:1348057/1‑150 (MQ=255)
                                                                                                                         atgatgTGTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTa                           <  2:3162139/150‑1 (MQ=255)
                                                                                                                           gatgTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCGGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAAt                         >  2:4327098/1‑150 (MQ=255)
                                                                                                                           gatgTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAAt                         <  1:3854734/150‑1 (MQ=255)
                                                                                                                           gatgTTACCGCGTCGTGATCCAGACTATAGGCCGGTCATTGTTATCTTTTCACTGTTGGCGTACATTCACGAAATTAACGGAATCATGTTCCTACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCAGTGTTGCAGGCGTAAt                         <  2:3565583/150‑1 (MQ=255)
                                                                                                                               tttCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGAtt                     <  1:74332/150‑1 (MQ=255)
                                                                                                                                ttCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTg                    >  1:589678/1‑150 (MQ=255)
                                                                                                                                 tCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGc                   <  2:3855362/150‑1 (MQ=255)
                                                                                                                                      gTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATTATTGCGATAc              >  1:2255397/1‑150 (MQ=255)
                                                                                                                                      gTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATAc              >  1:950192/1‑150 (MQ=255)
                                                                                                                                       tCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATACt             <  1:462496/150‑1 (MQ=255)
                                                                                                                                          tGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATACTGGt          <  1:720129/150‑1 (MQ=255)
                                                                                                                                             tCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATACTGGTGAg       <  2:3941740/150‑1 (MQ=255)
                                                                                                                                               cAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATACTGGTGAGtt     <  1:2524442/150‑1 (MQ=255)
                                                                                                                                                  |                                                                                                                                                     
AGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATACTGGTGAGTTGCC  >  CP009273/2263997‑2264292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATACTGGTGAGTTGCC  >  CP009273/2263995‑2264292
                                                                                                                                                    |                                                                                                                                                     
GAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGAC                                                                                                                                                      >  SRR19973080.4151247/1‑150 (MQ=60)
   GCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTAT                                                                                                                                                   <  SRR19973080.3265980/150‑1 (MQ=60)
    CACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATA                                                                                                                                                  <  SRR19973080.2520826/150‑1 (MQ=60)
    CACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATA                                                                                                                                                  >  SRR19973080.3991119/1‑150 (MQ=60)
     ACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAG                                                                                                                                                 <  SRR19973080.429553/150‑1 (MQ=60)
     ACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAG                                                                                                                                                 <  SRR19973080.2704992/150‑1 (MQ=60)
     ACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAG                                                                                                                                                 <  SRR19973080.2705022/150‑1 (MQ=60)
        CCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCC                                                                                                                                              <  SRR19973080.2363978/150‑1 (MQ=60)
        CCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCC                                                                                                                                              <  SRR19973080.3609450/150‑1 (MQ=60)
        CCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCC                                                                                                                                              <  SRR19973080.1597899/150‑1 (MQ=60)
         CGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCG                                                                                                                                             >  SRR19973080.1576675/1‑150 (MQ=60)
          GGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGTCTATAGTCCGG                                                                                                                                            >  SRR19973080.558282/1‑150 (MQ=60)
          GGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGG                                                                                                                                            >  SRR19973080.381157/1‑150 (MQ=60)
          GGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGG                                                                                                                                            >  SRR19973080.2758264/1‑150 (MQ=60)
           GGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGT                                                                                                                                           <  SRR19973080.3115619/150‑1 (MQ=60)
           GGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGT                                                                                                                                           <  SRR19973080.3500046/150‑1 (MQ=60)
           GGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGAAGTTTCCGCGTCTTGATCCAGACTATAGTCCGGT                                                                                                                                           >  SRR19973080.2440391/1‑150 (MQ=60)
            GTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTC                                                                                                                                          >  SRR19973080.632789/1‑150 (MQ=60)
             TTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCA                                                                                                                                         >  SRR19973080.2010281/1‑150 (MQ=60)
               CTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATT                                                                                                                                       <  SRR19973080.4469235/150‑1 (MQ=60)
                 CAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGT                                                                                                                                     <  SRR19973080.785605/150‑1 (MQ=60)
                  AGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTT                                                                                                                                    <  SRR19973080.3502966/150‑1 (MQ=60)
                    CCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTAT                                                                                                                                  <  SRR19973080.924696/150‑1 (MQ=60)
                     CGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATC                                                                                                                                 <  SRR19973080.640528/150‑1 (MQ=60)
                     CGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATC                                                                                                                                 >  SRR19973080.1010955/1‑150 (MQ=60)
                     CGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATC                                                                                                                                 <  SRR19973080.2080097/150‑1 (MQ=60)
                          CTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTA                                                                                                                            <  SRR19973080.2489081/150‑1 (MQ=60)
                          CTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTA                                                                                                                            <  SRR19973080.2489104/150‑1 (MQ=60)
                          CTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTA                                                                                                                            >  SRR19973080.860787/1‑150 (MQ=60)
                          CTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTA                                                                                                                            >  SRR19973080.4387052/1‑150 (MQ=60)
                           TAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAA                                                                                                                           <  SRR19973080.4103176/150‑1 (MQ=60)
                            AATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAA                                                                                                                          >  SRR19973080.798772/1‑150 (MQ=60)
                              TAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATG                                                                                                                        <  SRR19973080.4317297/150‑1 (MQ=60)
                              TAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATG                                                                                                                        <  SRR19973080.4317781/150‑1 (MQ=60)
                               AAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGT                                                                                                                       <  SRR19973080.2480185/150‑1 (MQ=60)
                                AACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTT                                                                                                                      >  SRR19973080.1426035/1‑150 (MQ=60)
                                 ACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTG                                                                                                                     >  SRR19973080.437075/1‑150 (MQ=60)
                                 ACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTG                                                                                                                     >  SRR19973080.4014850/1‑150 (MQ=60)
                                 ACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTG                                                                                                                     >  SRR19973080.436953/1‑150 (MQ=60)
                                 ACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTG                                                                                                                     <  SRR19973080.2290621/150‑1 (MQ=60)
                                  CCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGT                                                                                                                    <  SRR19973080.4040611/150‑1 (MQ=60)
                                  CCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGT                                                                                                                    <  SRR19973080.3674013/150‑1 (MQ=60)
                                  CCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGT                                                                                                                    >  SRR19973080.3479054/1‑150 (MQ=60)
                                  CCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGT                                                                                                                    >  SRR19973080.3478669/1‑150 (MQ=60)
                                       TGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAA                                                                                                               >  SRR19973080.2476795/1‑150 (MQ=60)
                                            GCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCA                                                                                                          <  SRR19973080.3961242/150‑1 (MQ=60)
                                            GCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGGGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCGATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCA                                                                                                          >  SRR19973080.3329452/1‑150 (MQ=60)
                                                TCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAA                                                                                                      >  SRR19973080.1118997/1‑150 (MQ=60)
                                                   CTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATT                                                                                                   <  SRR19973080.2191143/150‑1 (MQ=60)
                                                   CTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATT                                                                                                   <  SRR19973080.4477732/150‑1 (MQ=60)
                                                    TTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTA                                                                                                  <  SRR19973080.744133/150‑1 (MQ=60)
                                                    TTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTA                                                                                                  <  SRR19973080.744136/150‑1 (MQ=60)
                                                       GCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACG                                                                                               >  SRR19973080.197034/1‑150 (MQ=60)
                                                       GCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACG                                                                                               >  SRR19973080.197046/1‑150 (MQ=60)
                                                        CTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGG                                                                                              >  SRR19973080.2013315/1‑150 (MQ=60)
                                                        CTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGG                                                                                              >  SRR19973080.1107741/1‑150 (MQ=60)
                                                        CTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGG                                                                                              >  SRR19973080.1107555/1‑150 (MQ=60)
                                                          ATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAA                                                                                            >  SRR19973080.48100/1‑150 (MQ=60)
                                                          ATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAA                                                                                            <  SRR19973080.2472516/150‑1 (MQ=60)
                                                              TGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCAT                                                                                        >  SRR19973080.1308772/1‑150 (MQ=60)
                                                              TGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCAT                                                                                        >  SRR19973080.4040270/1‑150 (MQ=60)
                                                                ACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGT                                                                                      <  SRR19973080.1844646/150‑1 (MQ=60)
                                                                ACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGT                                                                                      <  SRR19973080.1844667/150‑1 (MQ=60)
                                                                 CGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTT                                                                                     <  SRR19973080.488975/150‑1 (MQ=60)
                                                                  GAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTC                                                                                    <  SRR19973080.265752/150‑1 (MQ=60)
                                                                        ACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGC                                                                              >  SRR19973080.4307618/1‑150 (MQ=60)
                                                                            AAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTC                                                                          >  SRR19973080.1054744/1‑150 (MQ=60)
                                                                              AGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCC                                                                        >  SRR19973080.3271664/1‑150 (MQ=60)
                                                                                 ACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAAT                                                                     <  SRR19973080.630939/150‑1 (MQ=60)
                                                                                  CTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATT                                                                    <  SRR19973080.1098543/150‑1 (MQ=60)
                                                                                  CTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATT                                                                    <  SRR19973080.1040306/150‑1 (MQ=60)
                                                                                  CTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATT                                                                    <  SRR19973080.1040379/150‑1 (MQ=60)
                                                                                   TGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTT                                                                   <  SRR19973080.637988/150‑1 (MQ=60)
                                                                                         GGCAGTGCTTTTTTGTTTTCATTCATCAGAGACAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACG                                                             <  SRR19973080.776453/150‑1 (MQ=60)
                                                                                         GGCAGTGCTTTTTTGTTTTCATTCATCAGAGAATATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACG                                                             <  SRR19973080.3514643/150‑1 (MQ=60)
                                                                                         GGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTAGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACG                                                             <  SRR19973080.974593/150‑1 (MQ=60)
                                                                                           CAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTA                                                           >  SRR19973080.2840937/1‑150 (MQ=60)
                                                                                           CAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTA                                                           >  SRR19973080.2840660/1‑150 (MQ=60)
                                                                                              TGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCCATTTTGGACGTAAGC                                                        >  SRR19973080.777606/1‑150 (MQ=60)
                                                                                                 TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCC                                                     >  SRR19973080.3591537/1‑150 (MQ=60)
                                                                                                 TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCC                                                     >  SRR19973080.3591503/1‑150 (MQ=60)
                                                                                                 TTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCC                                                     >  SRR19973080.3591441/1‑150 (MQ=60)
                                                                                                   TTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTG                                                   <  SRR19973080.326038/150‑1 (MQ=60)
                                                                                                    TTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGC                                                  <  SRR19973080.2584075/150‑1 (MQ=60)
                                                                                                    TTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGC                                                  <  SRR19973080.305299/150‑1 (MQ=60)
                                                                                                      TGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTT                                                <  SRR19973080.1886252/150‑1 (MQ=60)
                                                                                                      TGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTT                                                >  SRR19973080.1763175/1‑150 (MQ=60)
                                                                                                      TGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTT                                                >  SRR19973080.4563312/1‑150 (MQ=60)
                                                                                                        TTTTCATTCCTCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTAC                                              <  SRR19973080.3966672/150‑1 (MQ=60)
                                                                                                        TTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTAC                                              <  SRR19973080.3968356/150‑1 (MQ=60)
                                                                                                        TTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTAC                                              <  SRR19973080.4113605/150‑1 (MQ=60)
                                                                                                           TCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTG                                           >  SRR19973080.3830804/1‑150 (MQ=60)
                                                                                                               TCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATT                                       <  SRR19973080.1045336/150‑1 (MQ=60)
                                                                                                               TCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATT                                       <  SRR19973080.1047565/150‑1 (MQ=60)
                                                                                                                CATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTG                                      >  SRR19973080.3797805/1‑150 (MQ=60)
                                                                                                                             GATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAAT                         <  SRR19973080.3876180/150‑1 (MQ=60)
                                                                                                                             GATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAAT                         <  SRR19973080.3875940/150‑1 (MQ=60)
                                                                                                                                 TTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATT                     <  SRR19973080.75056/150‑1 (MQ=60)
                                                                                                                                  TTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTG                    >  SRR19973080.593341/1‑150 (MQ=60)
                                                                                                                                        GTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATTATTGCGATAC              >  SRR19973080.2267726/1‑150 (MQ=60)
                                                                                                                                        GTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATAC              >  SRR19973080.955972/1‑150 (MQ=60)
                                                                                                                                         TCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATACT             <  SRR19973080.465415/150‑1 (MQ=60)
                                                                                                                                         TCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATACT             <  SRR19973080.466069/150‑1 (MQ=60)
                                                                                                                                          CTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATACTG            >  SRR19973080.2414154/1‑150 (MQ=60)
                                                                                                                                          CTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATACTG            >  SRR19973080.2414176/1‑150 (MQ=60)
                                                                                                                                            TGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATACTGGT          <  SRR19973080.724276/150‑1 (MQ=60)
                                                                                                                                                CCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATACTGGTGAGT      <  SRR19973080.3201636/150‑1 (MQ=60)
                                                                                                                                                 CAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATACTGGTGAGTT     <  SRR19973080.2538330/150‑1 (MQ=60)
                                                                                                                                                 CAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATACTGGTGAGTT     <  SRR19973080.2538351/150‑1 (MQ=60)
                                                                                                                                                   GACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATACTGGTGAGTTGC   <  SRR19973080.3337120/150‑1 (MQ=60)
                                                                                                                                                   GACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATACTGGTGAGTTGC   <  SRR19973080.3336353/150‑1 (MQ=60)
                                                                                                                                                    GCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATACTGGTGAGTTGCC  <  SRR19973080.4157695/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                     
GAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATACTGGTGAGTTGCC  >  CP009273/2263995‑2264292

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑