Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F52 I0 R1 779 320.1 10410758 96.6% 10056792 149.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 2,264,143 G→A 27.9% intergenic (+119/‑62) mepS → / → rtn murein DD‑endopeptidase, space‑maker hydrolase, mutational suppressor of prc thermosensitivity, outer membrane lipoprotein/resistance protein for phages lambda and N4, putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092732,264,1430GA27.9% 588.7 / 267.0 319intergenic (+119/‑62)mepS/rtnmurein DD‑endopeptidase, space‑maker hydrolase, mutational suppressor of prc thermosensitivity, outer membrane lipoprotein/resistance protein for phages lambda and N4, putative membrane‑anchored cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base G (114/116);  new base A (40/49);  total (154/165)
Fisher's exact test for biased strand distribution p-value = 5.32e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

Only 100 of 324 total aligned reads displayed.

GAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATACTGGTGAGTTGC  >  CP009273/2263995‑2264291
                                                                                                                                                    |                                                                                                                                                    
gAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAgg                                                                                                                                                      >  1:48283/1‑149 (MQ=255)
   gCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCta                                                                                                                                                   >  1:114176/1‑149 (MQ=255)
   gCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCta                                                                                                                                                   <  1:1360661/149‑1 (MQ=255)
    cACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCtat                                                                                                                                                  <  1:608647/149‑1 (MQ=255)
     aCGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCtata                                                                                                                                                 >  1:35419/1‑149 (MQ=255)
      cGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAg                                                                                                                                                <  1:3615785/149‑1 (MQ=255)
        ccGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTc                                                                                                                                              <  2:4088615/149‑1 (MQ=255)
         cGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTcc                                                                                                                                             <  1:1803445/149‑1 (MQ=255)
          gggTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCg                                                                                                                                            >  2:1719200/1‑149 (MQ=255)
           ggTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCgg                                                                                                                                           <  1:1166046/149‑1 (MQ=255)
            gTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGt                                                                                                                                          >  2:2336680/1‑149 (MQ=255)
            gTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGt                                                                                                                                          <  2:677808/149‑1 (MQ=255)
             ttCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTc                                                                                                                                         <  1:2418479/149‑1 (MQ=255)
             ttCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTc                                                                                                                                         <  1:1713921/149‑1 (MQ=255)
              tctcAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCa                                                                                                                                        <  2:1361355/149‑1 (MQ=255)
                tcATCCGCATATAATAAACCGTTTGGATTCAATCCCTTTGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATTTTTCCGCGTATTGATCCAGGCTATAGTCCGGTCAtt                                                                                                                                      <  2:3696397/149‑1 (MQ=255)
                tcAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCAtt                                                                                                                                      >  1:5035067/1‑149 (MQ=255)
                   gCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGtt                                                                                                                                   >  2:4825828/1‑149 (MQ=255)
                    ccGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTa                                                                                                                                  <  1:2604293/149‑1 (MQ=255)
                      gCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATc                                                                                                                                >  2:1981124/1‑149 (MQ=255)
                       cAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCt                                                                                                                               <  1:397406/149‑1 (MQ=255)
                         gCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCttt                                                                                                                             <  2:3241046/149‑1 (MQ=255)
                         gCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCttt                                                                                                                             <  1:2889624/149‑1 (MQ=255)
                            aataaACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTaa                                                                                                                          >  1:4671610/1‑149 (MQ=255)
                              taaACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAAt                                                                                                                        <  2:1238847/149‑1 (MQ=255)
                               aaaCCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAAtg                                                                                                                       >  2:2966695/1‑149 (MQ=255)
                                aaCCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAAtgt                                                                                                                      >  2:2622695/1‑149 (MQ=255)
                                aaCCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAAtgt                                                                                                                      >  1:2271479/1‑149 (MQ=255)
                                 aCCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAAtgtt                                                                                                                     <  1:971449/149‑1 (MQ=255)
                                    gTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTc                                                                                                                  >  1:1229291/1‑149 (MQ=255)
                                     tttGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCg                                                                                                                 >  2:1009748/1‑149 (MQ=255)
                                         gATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAAt                                                                                                             <  1:5173473/149‑1 (MQ=255)
                                           tGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAAttt                                                                                                           <  1:3037470/149‑1 (MQ=255)
                                           tGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAAttt                                                                                                           >  1:525446/1‑149 (MQ=255)
                                            gCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTc                                                                                                          <  1:1098276/149‑1 (MQ=255)
                                              aaTCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAg                                                                                                        <  1:4387271/149‑1 (MQ=255)
                                                   cTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAAt                                                                                                   <  1:4628351/149‑1 (MQ=255)
                                                        cTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACg                                                                                              <  2:1109165/149‑1 (MQ=255)
                                                        cTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACg                                                                                              <  1:3169784/149‑1 (MQ=255)
                                                           tCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGaa                                                                                           >  1:2181723/1‑149 (MQ=255)
                                                              tGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCa                                                                                        <  2:3057099/149‑1 (MQ=255)
                                                                aCGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATg                                                                                      <  2:539696/149‑1 (MQ=255)
                                                                  gAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGtt                                                                                    <  2:4178360/149‑1 (MQ=255)
                                                                   aGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTc                                                                                   >  1:787770/1‑149 (MQ=255)
                                                                         cagAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATAcgc                                                                             <  2:4507258/147‑1 (MQ=255)
                                                                           gAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACgcgc                                                                           <  2:4922199/149‑1 (MQ=255)
                                                                              aGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTcc                                                                        >  2:2760423/1‑149 (MQ=255)
                                                                               gCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTccc                                                                       <  1:2934312/149‑1 (MQ=255)
                                                                                 aCTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCaa                                                                     >  2:4612293/1‑149 (MQ=255)
                                                                                 aCTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCaa                                                                     >  1:3318802/1‑149 (MQ=255)
                                                                                     cTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAAtttt                                                                 <  1:2457584/149‑1 (MQ=255)
                                                                                     cTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAAtttt                                                                 <  1:2643124/149‑1 (MQ=255)
                                                                                             gTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTaa                                                         >  2:3838093/1‑149 (MQ=255)
                                                                                             gTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTaa                                                         >  1:2002931/1‑149 (MQ=255)
                                                                                             gTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGACTCATGTTCATACGCGCTCCCAATTTTGGACGTaa                                                         >  2:224656/1‑149 (MQ=255)
                                                                                               gCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGc                                                       <  1:1441996/149‑1 (MQ=255)
                                                                                                cTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCt                                                      <  2:1932683/149‑1 (MQ=255)
                                                                                                 ttttttGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTAGTAATTTCAGTAAATTAACGGAATCATGTTCATAAGCGCTCCCAATTTTGGACGTAAGCTc                                                     >  1:3682890/1‑149 (MQ=255)
                                                                                                    tttGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTg                                                  <  1:2709630/149‑1 (MQ=255)
                                                                                                    tttGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGCAATTTCAGGAAATTAACGGAATCATGTTCATACGAGCTCCCAATTTTGGACGTAAGCTCCTg                                                  >  1:4052540/1‑149 (MQ=255)
                                                                                                       gTTTTCAGTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCtt                                               <  2:2072521/149‑1 (MQ=255)
                                                                                                        ttttCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTa                                              >  1:4707097/1‑149 (MQ=255)
                                                                                                         tttCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTAc                                             >  1:5065566/1‑149 (MQ=255)
                                                                                                           tcattcatCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCt                                           <  2:424653/149‑1 (MQ=255)
                                                                                                           tcattcatCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCt                                           <  2:3035136/149‑1 (MQ=255)
                                                                                                            cattcatCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTg                                          >  1:1595633/1‑149 (MQ=255)
                                                                                                                catcaGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCAtt                                      <  1:4228614/149‑1 (MQ=255)
                                                                                                                 atcaGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCAttg                                     >  2:585433/1‑149 (MQ=255)
                                                                                                                 atcaGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCAttg                                     >  2:3184416/1‑149 (MQ=255)
                                                                                                                  tcaGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCAttgt                                    >  2:4399193/1‑149 (MQ=255)
                                                                                                                  tcaGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCAttgt                                    >  1:2806770/1‑149 (MQ=255)
                                                                                                                  tcaGAGAAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCAttgt                                    >  2:3772813/1‑149 (MQ=255)
                                                                                                                   caGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCAttgtt                                   <  2:2271479/149‑1 (MQ=255)
                                                                                                                     gagaAAATGATGTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGc                                 >  1:3447363/1‑149 (MQ=255)
                                                                                                                      agaAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCa                                <  2:5012607/149‑1 (MQ=255)
                                                                                                                      agaAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCa                                >  2:1302622/1‑149 (MQ=255)
                                                                                                                        aaaaTGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAgg                              >  2:3220914/1‑149 (MQ=255)
                                                                                                                            tgatgTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTa                          >  2:1413738/1‑149 (MQ=255)
                                                                                                                             gatgTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTaa                         <  1:4952775/149‑1 (MQ=255)
                                                                                                                              atgTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAAt                        <  2:1500896/149‑1 (MQ=255)
                                                                                                                              atgTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAAt                        >  2:4112661/1‑149 (MQ=255)
                                                                                                                               tgTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATg                       >  1:1664758/1‑149 (MQ=255)
                                                                                                                               tgTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATg                       >  1:1042979/1‑149 (MQ=255)
                                                                                                                               tgTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATg                       >  2:3532009/1‑149 (MQ=255)
                                                                                                                               tgTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATg                       >  2:4778620/1‑149 (MQ=255)
                                                                                                                                gTTTCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGa                      <  1:23566/149‑1 (MQ=255)
                                                                                                                                 tttCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGAt                     <  2:2469245/149‑1 (MQ=255)
                                                                                                                                 tttCCGCGTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGAt                     <  1:1727053/149‑1 (MQ=255)
                                                                                                                                       cgTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGAt               <  2:1372304/149‑1 (MQ=255)
                                                                                                                                       cgTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAACTGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCACTTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGAt               <  2:4483897/149‑1 (MQ=255)
                                                                                                                                        gTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACCGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATa              >  1:2084234/1‑149 (MQ=255)
                                                                                                                                        gTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATa              >  1:1882181/1‑149 (MQ=255)
                                                                                                                                        gTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATa              <  1:4946272/149‑1 (MQ=255)
                                                                                                                                        gTCTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATa              >  1:1513299/1‑149 (MQ=255)
                                                                                                                                         tCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATAc             >  2:332736/1‑149 (MQ=255)
                                                                                                                                         tCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATAc             <  2:1650013/149‑1 (MQ=255)
                                                                                                                                          cTTGATCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATACt            <  2:1189923/149‑1 (MQ=255)
                                                                                                                                              aTCCAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATACTGGTg        >  1:836075/1‑149 (MQ=255)
                                                                                                                                                 cAGACTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATACTGGTGAGt     <  2:3747501/149‑1 (MQ=255)
                                                                                                                                                   ggCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATACTGGTGAGTTg   >  2:950689/1‑149 (MQ=255)
                                                                                                                                                    |                                                                                                                                                    
GAAGCACGCCGGGTTCTCAGCCGCAGCTAATAAACCGTTTGGATGCAATCCCTTGGCTATCCTGACGAGTTAACTGAAAGCACTGCTTAGGCAGTGCTTTTTTGTTTTCATTCATCAGAGAAAATGATGTTTCCGCGTCTTGATCCAGGCTATAGTCCGGTCATTGTTATCTTTTAAATGTTGTCGTAATTTCAGGAAATTAACGGAATCATGTTCATACGCGCTCCCAATTTTGGACGTAAGCTCCTGCTTACCTGCATTGTTGCAGGCGTAATGATTGCGATACTGGTGAGTTGC  >  CP009273/2263995‑2264291

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

N/A