Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F361 I0 R1 1353 250.7 8230166 96.0% 7900959 148.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 319,024 C→A 100% R326S (CGC→AGC)  ykgF → ferridoxin‑like LutB family protein; putative electron transport chain YkgEFG component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273319,0240CA98.9% 598.6 / ‑1.9 184R326S (CGC→AGC) ykgFferridoxin‑like LutB family protein; putative electron transport chain YkgEFG component
Reads supporting (aligned to +/- strand):  ref base C (1/1);  new base A (68/114);  total (69/115)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 186 total aligned reads displayed.

GCGCGAAGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGT  >  CP009273/318879‑319169
                                                                                                                                                 |                                                                                                                                                 
 cgcgAAGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCa                                                                                                                                               <  1:122308/149‑1 (MQ=255)
 cgcgAAGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCa                                                                                                                                               <  2:2179519/149‑1 (MQ=255)
 cgcgAAGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCa                                                                                                                                               <  1:608074/149‑1 (MQ=255)
     aaGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATAtt                                                                                                                                           <  1:920384/149‑1 (MQ=255)
      aGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTg                                                                                                                                          <  1:3861787/149‑1 (MQ=255)
       gCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTgg                                                                                                                                         <  2:63601/149‑1 (MQ=255)
        cTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGc                                                                                                                                        <  1:2731857/149‑1 (MQ=255)
         tGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCg                                                                                                                                       <  1:1499552/149‑1 (MQ=255)
           ggCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGt                                                                                                                                     <  2:4050927/149‑1 (MQ=255)
           ggCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGt                                                                                                                                     <  2:1963930/149‑1 (MQ=255)
            gCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTc                                                                                                                                    <  2:2346093/149‑1 (MQ=255)
            gCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTc                                                                                                                                    <  2:1409841/149‑1 (MQ=255)
            gCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTc                                                                                                                                    <  2:1409852/149‑1 (MQ=255)
              aCGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCAt                                                                                                                                  <  2:700126/149‑1 (MQ=255)
                gTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATgg                                                                                                                                >  1:2962472/1‑149 (MQ=255)
                 ttGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGa                                                                                                                               >  1:2173829/1‑149 (MQ=255)
                 ttGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGa                                                                                                                               <  1:2523805/149‑1 (MQ=255)
                 ttGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTACGGCATATAGCCATATTGGCGGTCATGGa                                                                                                                               >  2:62186/1‑149 (MQ=255)
                   gATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGata                                                                                                                             <  2:3300587/149‑1 (MQ=255)
                   gATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGata                                                                                                                             <  2:3507851/149‑1 (MQ=255)
                     tGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATg                                                                                                                           <  2:631777/149‑1 (MQ=255)
                        tCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGct                                                                                                                        >  2:662140/1‑149 (MQ=255)
                        tCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGct                                                                                                                        <  1:623223/149‑1 (MQ=255)
                        tCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGct                                                                                                                        >  2:863345/1‑149 (MQ=255)
                          cTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGctct                                                                                                                      >  1:1637441/1‑149 (MQ=255)
                           tGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTa                                                                                                                     <  2:2206044/149‑1 (MQ=255)
                             aaGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTAtt                                                                                                                   <  2:2314921/149‑1 (MQ=255)
                                 gTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATc                                                                                                               <  2:3102931/149‑1 (MQ=255)
                                  tttCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATcc                                                                                                              <  2:1767391/149‑1 (MQ=255)
                                   ttCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCa                                                                                                             <  2:3057687/149‑1 (MQ=255)
                                      aTCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAggg                                                                                                          >  2:1008941/1‑149 (MQ=255)
                                      aTCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAggg                                                                                                          >  2:2095306/1‑149 (MQ=255)
                                       tCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGc                                                                                                         <  1:488087/149‑1 (MQ=255)
                                        cTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGcc                                                                                                        <  1:1416291/149‑1 (MQ=255)
                                           gTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAAt                                                                                                     <  1:3866365/149‑1 (MQ=255)
                                           gTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAAt                                                                                                     >  2:3159967/1‑149 (MQ=255)
                                           gTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAAt                                                                                                     >  1:2262651/1‑149 (MQ=255)
                                           gTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAAt                                                                                                     >  2:3416143/1‑149 (MQ=255)
                                              aTTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTgg                                                                                                  >  2:1303563/1‑149 (MQ=255)
                                               ttGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTTGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGt                                                                                                 >  1:2944047/1‑149 (MQ=255)
                                                   cGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCgg                                                                                             <  2:1361922/149‑1 (MQ=255)
                                                    gATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGt                                                                                            <  1:347353/149‑1 (MQ=255)
                                                      tAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGa                                                                                          <  2:2712857/149‑1 (MQ=255)
                                                       aaCGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGAt                                                                                         >  2:1490890/1‑149 (MQ=255)
                                                        aCGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGAtt                                                                                        <  2:1172413/149‑1 (MQ=255)
                                                         cGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGAttt                                                                                       <  1:3119336/149‑1 (MQ=255)
                                                          gggCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTtc                                                                                      <  1:1670697/149‑1 (MQ=255)
                                                             cGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGATTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTcc                                                                                   <  2:27308/149‑1 (MQ=255)
                                                               ttCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGc                                                                                 <  1:1525378/149‑1 (MQ=255)
                                                                tCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCt                                                                                >  1:3528034/1‑149 (MQ=255)
                                                                    aGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACtt                                                                            >  2:2795592/1‑149 (MQ=255)
                                                                      gTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTgg                                                                          >  2:87657/1‑149 (MQ=255)
                                                                      gTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTgg                                                                          <  2:2557234/149‑1 (MQ=255)
                                                                           ggCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCt                                                                     <  2:23985/149‑1 (MQ=255)
                                                                           ggCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCt                                                                     <  2:1595156/149‑1 (MQ=255)
                                                                           ggCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCt                                                                     <  2:2103548/149‑1 (MQ=255)
                                                                           ggCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCt                                                                     <  1:472749/149‑1 (MQ=255)
                                                                           ggCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCt                                                                     <  1:472750/149‑1 (MQ=255)
                                                                               tctGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaa                                                                 >  1:2608907/1‑149 (MQ=255)
                                                                               tctGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaa                                                                 <  1:3541647/149‑1 (MQ=255)
                                                                               tctGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaa                                                                 >  1:1900072/1‑149 (MQ=255)
                                                                                   aaTTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagat                                                             <  2:1695721/149‑1 (MQ=255)
                                                                                         gggATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattttaaa                                                       <  2:1706424/149‑1 (MQ=255)
                                                                                         gggATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTACGCTACTTGGCGGCTAtaaagattttaaa                                                       <  1:2091217/149‑1 (MQ=255)
                                                                                          ggATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattttaaag                                                      >  1:3694710/1‑149 (MQ=255)
                                                                                           gATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattttaaaga                                                     >  2:2452236/1‑149 (MQ=255)
                                                                                            aTGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattttaaagat                                                    >  2:3840443/1‑149 (MQ=255)
                                                                                             tgtgCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattttaaagatt                                                   <  2:2216019/149‑1 (MQ=255)
                                                                                                 ctgcGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTAcc                                               <  1:2140786/149‑1 (MQ=255)
                                                                                                 ctgcGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTAcc                                               <  1:3385125/149‑1 (MQ=255)
                                                                                                  tgcGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTAccc                                              >  2:1714681/1‑149 (MQ=255)
                                                                                                  tgcGCTGGATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTAccc                                              <  2:1923115/149‑1 (MQ=255)
                                                                                                         tATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCt                                       >  1:487443/1‑149 (MQ=255)
                                                                                                              gCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATGTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGctct                                  >  2:1225174/1‑149 (MQ=255)
                                                                                                               cTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCtt                                 <  1:1838418/149‑1 (MQ=255)
                                                                                                                    gggCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGc                            >  1:3937296/1‑149 (MQ=255)
                                                                                                                      gCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGcac                          >  1:280967/1‑149 (MQ=255)
                                                                                                                      gCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGcac                          <  1:2647508/149‑1 (MQ=255)
                                                                                                                        ttGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAg                        <  1:1223861/149‑1 (MQ=255)
                                                                                                                        ttGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAg                        <  1:3276705/149‑1 (MQ=255)
                                                                                                                         tGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGc                       <  1:1474064/149‑1 (MQ=255)
                                                                                                                          gTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCt                      <  2:2902798/149‑1 (MQ=255)
                                                                                                                          gTATGAATACTGGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCt                      <  2:844205/149‑1 (MQ=255)
                                                                                                                           tATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCtt                     >  1:343090/1‑149 (MQ=255)
                                                                                                                               aaTACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGa                 <  1:1621452/149‑1 (MQ=255)
                                                                                                                                aTACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGAc                <  2:829639/149‑1 (MQ=255)
                                                                                                                                aTACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGAc                <  2:385146/149‑1 (MQ=255)
                                                                                                                                aTACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGAc                <  2:385133/149‑1 (MQ=255)
                                                                                                                                 tACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACa               <  2:1164681/149‑1 (MQ=255)
                                                                                                                                  aCTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACaa              >  2:1738219/1‑149 (MQ=255)
                                                                                                                                   cTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAAc             <  2:3624976/149‑1 (MQ=255)
                                                                                                                                    ttGTCCGGCATATAGACATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACg            <  2:3928756/149‑1 (MQ=255)
                                                                                                                                     tGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACgt           >  1:3627027/1‑149 (MQ=255)
                                                                                                                                      gTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACgtg          <  2:2173829/149‑1 (MQ=255)
                                                                                                                                         cGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACgtgtgt       <  2:3018162/149‑1 (MQ=255)
                                                                                                                                           gCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTcc     >  2:925689/1‑149 (MQ=255)
                                                                                                                                           gCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTcc     <  1:2403557/149‑1 (MQ=255)
                                                                                                                                           gCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTcc     >  2:541098/1‑149 (MQ=255)
                                                                                                                                             atatAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCgg   <  2:1302297/149‑1 (MQ=255)
                                                                                                                                              tataGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGt  >  2:126450/1‑149 (MQ=255)
                                                                                                                                                 |                                                                                                                                                 
GCGCGAAGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGT  >  CP009273/318879‑319169

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
ACCGCGCGAAGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGC  >  CP009273/318876‑319171
                                                                                                                                                    |                                                                                                                                                   
ACCGCGCGAAGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAG                                                                                                                                                    <  SRR19973068.2393405/150‑1 (MQ=60)
ACCGCGCGAAGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAG                                                                                                                                                    <  SRR19973068.3526136/150‑1 (MQ=60)
  CGCGCGAAGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCC                                                                                                                                                  >  SRR19973068.3968044/1‑150 (MQ=60)
    CGCGAAGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCAT                                                                                                                                                <  SRR19973068.123451/150‑1 (MQ=60)
    CGCGAAGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCAT                                                                                                                                                <  SRR19973068.354617/150‑1 (MQ=60)
    CGCGAAGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCAT                                                                                                                                                <  SRR19973068.367201/150‑1 (MQ=60)
    CGCGAAGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCAT                                                                                                                                                <  SRR19973068.612146/150‑1 (MQ=60)
        AAGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTG                                                                                                                                            <  SRR19973068.926578/150‑1 (MQ=60)
         AGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGG                                                                                                                                           <  SRR19973068.3885103/150‑1 (MQ=60)
           CTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCG                                                                                                                                         <  SRR19973068.2748707/150‑1 (MQ=60)
            TGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGG                                                                                                                                        <  SRR19973068.1507952/150‑1 (MQ=60)
            TGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGG                                                                                                                                        <  SRR19973068.3385946/150‑1 (MQ=60)
            TGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGG                                                                                                                                        <  SRR19973068.1509412/150‑1 (MQ=60)
                 ACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATG                                                                                                                                   <  SRR19973068.1533641/150‑1 (MQ=60)
                  CGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGG                                                                                                                                  >  SRR19973068.1451646/1‑150 (MQ=60)
                  CGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGG                                                                                                                                  >  SRR19973068.2980844/1‑150 (MQ=60)
                   GTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGA                                                                                                                                 >  SRR19973068.2188059/1‑150 (MQ=60)
                    TTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGAT                                                                                                                                <  SRR19973068.2539852/150‑1 (MQ=60)
                          GTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATATCCATATTGGCGGTCATGGATATGGCT                                                                                                                          >  SRR19973068.1589530/1‑150 (MQ=60)
                          GTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCT                                                                                                                          <  SRR19973068.562668/150‑1 (MQ=60)
                           TCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTC                                                                                                                         <  SRR19973068.627356/150‑1 (MQ=60)
                            CCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCT                                                                                                                        >  SRR19973068.1648103/1‑150 (MQ=60)
                                        CATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGG                                                                                                            >  SRR19973068.2747678/1‑150 (MQ=60)
                                          TCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCC                                                                                                          <  SRR19973068.491513/150‑1 (MQ=60)
                                          TCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCC                                                                                                          <  SRR19973068.3732792/150‑1 (MQ=60)
                                          TCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCC                                                                                                          <  SRR19973068.491325/150‑1 (MQ=60)
                                           CTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCA                                                                                                         <  SRR19973068.1425482/150‑1 (MQ=60)
                                             GGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAAT                                                                                                       >  SRR19973068.2277346/1‑150 (MQ=60)
                                              GTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATT                                                                                                      <  SRR19973068.3888377/150‑1 (MQ=60)
                                              GTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATT                                                                                                      <  SRR19973068.3889711/150‑1 (MQ=60)
                                                 ATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTTGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGT                                                                                                   >  SRR19973068.2962319/1‑150 (MQ=60)
                                                 ATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGT                                                                                                   <  SRR19973068.3454576/150‑1 (MQ=60)
                                                     TCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGG                                                                                               <  SRR19973068.1636444/150‑1 (MQ=60)
                                                       GATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTG                                                                                             <  SRR19973068.349541/150‑1 (MQ=60)
                                                       GATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTG                                                                                             >  SRR19973068.207448/1‑150 (MQ=60)
                                                            CGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTC                                                                                        <  SRR19973068.3138414/150‑1 (MQ=60)
                                                             GGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCT                                                                                       <  SRR19973068.1681479/150‑1 (MQ=60)
                                                              GGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTC                                                                                      >  SRR19973068.3622921/1‑150 (MQ=60)
                                                               GCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTTGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCC                                                                                     <  SRR19973068.2646039/150‑1 (MQ=60)
                                                                  TTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCT                                                                                  >  SRR19973068.3549494/1‑150 (MQ=60)
                                                                  TTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCT                                                                                  <  SRR19973068.1535412/150‑1 (MQ=60)
                                                                              GGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTA                                                                      <  SRR19973068.476085/150‑1 (MQ=60)
                                                                              GGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTA                                                                      <  SRR19973068.476086/150‑1 (MQ=60)
                                                                                 CTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAA                                                                   >  SRR19973068.1912693/1‑150 (MQ=60)
                                                                                 CTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAA                                                                   >  SRR19973068.2625353/1‑150 (MQ=60)
                                                                                  TCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAA                                                                  <  SRR19973068.3563182/150‑1 (MQ=60)
                                                                                      AATTTCGGGATGTTCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATT                                                              <  SRR19973068.2224416/150‑1 (MQ=60)
                                                                                            GGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAG                                                        >  SRR19973068.3716972/1‑150 (MQ=60)
                                                                                            GGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTACGCTACTTGGCGGCTATAAAGATTTTAAAG                                                        <  SRR19973068.2104815/150‑1 (MQ=60)
                                                                                                    CTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCC                                                <  SRR19973068.2154810/150‑1 (MQ=60)
                                                                                                    CTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCC                                                <  SRR19973068.2154798/150‑1 (MQ=60)
                                                                                                    CTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCC                                                <  SRR19973068.3405649/150‑1 (MQ=60)
                                                                                                    CTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCC                                                <  SRR19973068.2154612/150‑1 (MQ=60)
                                                                                                     TGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCT                                               >  SRR19973068.3087688/1‑150 (MQ=60)
                                                                                                           GTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCT                                         >  SRR19973068.490864/1‑150 (MQ=60)
                                                                                                                 GCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTT                                   <  SRR19973068.2436738/150‑1 (MQ=60)
                                                                                                                  CTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTT                                  <  SRR19973068.1850578/150‑1 (MQ=60)
                                                                                                                  CTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTT                                  <  SRR19973068.1850542/150‑1 (MQ=60)
                                                                                                                  CTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTT                                  <  SRR19973068.486892/150‑1 (MQ=60)
                                                                                                                    GCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTAT                                <  SRR19973068.2812933/150‑1 (MQ=60)
                                                                                                                     CGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATG                               >  SRR19973068.1385357/1‑150 (MQ=60)
                                                                                                                      GGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGC                              >  SRR19973068.3961135/1‑150 (MQ=60)
                                                                                                                      GGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGC                              >  SRR19973068.3961012/1‑150 (MQ=60)
                                                                                                                        GGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCAC                            >  SRR19973068.282905/1‑150 (MQ=60)
                                                                                                                        GGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCAC                            >  SRR19973068.282904/1‑150 (MQ=60)
                                                                                                                         GCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACA                           <  SRR19973068.2664105/150‑1 (MQ=60)
                                                                                                                          CTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGTATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAG                          <  SRR19973068.2617848/150‑1 (MQ=60)
                                                                                                                           TTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGC                         <  SRR19973068.3296811/150‑1 (MQ=60)
                                                                                                                           TTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGC                         <  SRR19973068.3296548/150‑1 (MQ=60)
                                                                                                                           TTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGC                         <  SRR19973068.1231999/150‑1 (MQ=60)
                                                                                                                            TGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCT                        <  SRR19973068.1483753/150‑1 (MQ=60)
                                                                                                                             GTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTT                       >  SRR19973068.1797004/1‑150 (MQ=60)
                                                                                                                             GTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTT                       >  SRR19973068.345238/1‑150 (MQ=60)
                                                                                                                             GTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTT                       >  SRR19973068.344458/1‑150 (MQ=60)
                                                                                                                                  AATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGAC                  <  SRR19973068.1632041/150‑1 (MQ=60)
                                                                                                                                   ATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACA                 <  SRR19973068.937797/150‑1 (MQ=60)
                                                                                                                                       TTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGT             >  SRR19973068.3649025/1‑150 (MQ=60)
                                                                                                                                              GCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCG      <  SRR19973068.2418822/150‑1 (MQ=60)
                                                                                                                                              GCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCG      <  SRR19973068.62915/150‑1 (MQ=60)
                                                                                                                                              GCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCG      <  SRR19973068.63682/150‑1 (MQ=60)
                                                                                                                                                  ATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGC  >  SRR19973068.369464/1‑150 (MQ=60)
                                                                                                                                                  ATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGC  <  SRR19973068.1273223/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                   
ACCGCGCGAAGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGC  >  CP009273/318876‑319171

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑