Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F342 I0 R1 1331 225.9 7641038 95.8% 7320114 147.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 319,024 C→A 100% R326S (CGC→AGC)  ykgF → ferridoxin‑like LutB family protein; putative electron transport chain YkgEFG component

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273319,0240CA96.9% 606.3 / 9.1 198R326S (CGC→AGC) ykgFferridoxin‑like LutB family protein; putative electron transport chain YkgEFG component
Reads supporting (aligned to +/- strand):  ref base C (1/5);  new base A (89/102);  total (91/107)
Fisher's exact test for biased strand distribution p-value = 2.22e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.55e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 200 total aligned reads displayed.

CGAAGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGG  >  CP009273/318882‑319168
                                                                                                                                              |                                                                                                                                                
cgcaGCTGGGCACGTTGATGGTCCTGTAGAGTTTCCTCTGGTTATTGTCGTGAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCat                                                                                                                                             <  2:2491155/145‑1 (MQ=255)
cgAAGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCat                                                                                                                                             <  2:3800646/148‑1 (MQ=255)
  aaGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCatat                                                                                                                                           >  1:459049/1‑148 (MQ=255)
  aaGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCatat                                                                                                                                           <  2:200497/148‑1 (MQ=255)
    gCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTg                                                                                                                                         <  1:798706/148‑1 (MQ=255)
    gCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTg                                                                                                                                         <  2:1753717/148‑1 (MQ=255)
         gCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGt                                                                                                                                    >  1:1787796/1‑148 (MQ=255)
          cACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTc                                                                                                                                   >  2:445633/1‑148 (MQ=255)
            cGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCAt                                                                                                                                 <  1:3539246/148‑1 (MQ=255)
            cGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCAt                                                                                                                                 >  1:2064845/1‑148 (MQ=255)
              ttGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATgg                                                                                                                               <  2:1908576/148‑1 (MQ=255)
                gATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGat                                                                                                                             <  2:954261/148‑1 (MQ=255)
                gATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGat                                                                                                                             <  1:2653341/148‑1 (MQ=255)
                   ggTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATg                                                                                                                          <  1:830896/148‑1 (MQ=255)
                    gTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATgg                                                                                                                         >  2:3805446/1‑148 (MQ=255)
                    gTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATgg                                                                                                                         <  1:1851436/148‑1 (MQ=255)
                        tGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGctct                                                                                                                     <  2:734168/148‑1 (MQ=255)
                        tGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGctct                                                                                                                     <  2:734614/148‑1 (MQ=255)
                         gAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTa                                                                                                                    >  2:2267558/1‑148 (MQ=255)
                         gAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTa                                                                                                                    >  2:341968/1‑148 (MQ=255)
                         gAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTa                                                                                                                    >  1:567024/1‑148 (MQ=255)
                          aaGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTAt                                                                                                                   <  2:3662358/148‑1 (MQ=255)
                          aaGAGTTTCATCTGGTTATTGTCGATAACGGGAGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTAt                                                                                                                   <  1:1392648/148‑1 (MQ=255)
                           agagTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTAtt                                                                                                                  >  1:3765145/1‑148 (MQ=255)
                            gagTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTAttt                                                                                                                 <  2:3358489/148‑1 (MQ=255)
                            gagTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTAttt                                                                                                                 <  2:1055004/148‑1 (MQ=255)
                              gTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTAt                                                                                                               <  1:2153524/148‑1 (MQ=255)
                               tttCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTTGCGGTCATGGATATGGCTCTATTTATc                                                                                                              >  1:2378703/1‑148 (MQ=255)
                               tttCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATc                                                                                                              <  2:1722823/148‑1 (MQ=255)
                               tttCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGACTCTATTTATc                                                                                                              <  2:1722804/148‑1 (MQ=255)
                                 tCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCa                                                                                                            >  1:3266936/1‑148 (MQ=255)
                                  cATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAg                                                                                                           <  1:1284036/148‑1 (MQ=255)
                                    tCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAggg                                                                                                         <  1:2586629/148‑1 (MQ=255)
                                     cTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGc                                                                                                        >  2:1062065/1‑148 (MQ=255)
                                       ggTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCa                                                                                                      >  2:1474484/1‑148 (MQ=255)
                                       ggTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCa                                                                                                      >  1:2838374/1‑148 (MQ=255)
                                       ggTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCa                                                                                                      >  2:1474469/1‑148 (MQ=255)
                                       ggTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCa                                                                                                      >  2:126498/1‑148 (MQ=255)
                                         ttATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAAt                                                                                                    <  2:2561049/148‑1 (MQ=255)
                                            ttGTCTATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTgg                                                                                                 >  2:72835/1‑148 (MQ=255)
                                            ttGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTgg                                                                                                 <  1:2932252/148‑1 (MQ=255)
                                             tGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGt                                                                                                >  2:1573135/1‑148 (MQ=255)
                                              gTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTg                                                                                               <  2:3568868/148‑1 (MQ=255)
                                                cGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCg                                                                                             <  2:1328394/148‑1 (MQ=255)
                                                 gATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCgg                                                                                            >  2:659168/1‑148 (MQ=255)
                                                   tAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTg                                                                                          <  2:2306880/148‑1 (MQ=255)
                                                   tAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTg                                                                                          >  2:2973242/1‑148 (MQ=255)
                                                    aaCGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGa                                                                                         >  2:1216983/1‑148 (MQ=255)
                                                    aaCGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGa                                                                                         <  2:32336/148‑1 (MQ=255)
                                                      cGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGAtt                                                                                       >  2:2158787/1‑148 (MQ=255)
                                                      cGGGCGTTCTGAGGTGCTGGCCTCAGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGTGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGATCTATTTATCCAGGGCCAATTGGTGCGGTGAtt                                                                                       >  2:3594655/1‑148 (MQ=255)
                                                       gggCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGAttt                                                                                      <  2:2409245/148‑1 (MQ=255)
                                                         gCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTtct                                                                                    >  1:62676/1‑148 (MQ=255)
                                                         gCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTtct                                                                                    <  2:2310041/148‑1 (MQ=255)
                                                          cGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTtctc                                                                                   >  2:2318837/1‑148 (MQ=255)
                                                              cTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCt                                                                               <  2:406311/148‑1 (MQ=255)
                                                               tGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTa                                                                              <  2:576013/148‑1 (MQ=255)
                                                                gAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTAc                                                                             <  2:1126482/148‑1 (MQ=255)
                                                                gAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTAc                                                                             >  2:1548799/1‑148 (MQ=255)
                                                                gAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTAc                                                                             <  1:991811/148‑1 (MQ=255)
                                                                    tGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGAGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTgt                                                                         >  1:2799240/1‑147 (MQ=255)
                                                                         gCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCt                                                                    >  1:872985/1‑148 (MQ=255)
                                                                            tctGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCtata                                                                 >  1:3039585/1‑148 (MQ=255)
                                                                             ctGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaa                                                                <  1:3215272/148‑1 (MQ=255)
                                                                               gAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaag                                                              <  2:3765145/148‑1 (MQ=255)
                                                                               gAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaag                                                              <  1:399475/148‑1 (MQ=255)
                                                                               gAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaag                                                              <  2:751094/148‑1 (MQ=255)
                                                                                 aTTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagat                                                            >  1:41008/1‑148 (MQ=255)
                                                                                  tttCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagatt                                                           >  1:2860811/1‑148 (MQ=255)
                                                                                      gtgATGTGCTGCGCTGTATTCGCTGCGGGGATTGTATGAATACTTGTCCGGCATATAGCCATATTGTCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattttaa                                                       <  2:2376275/146‑1 (MQ=255)
                                                                                      gggATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattttaa                                                       <  2:867535/148‑1 (MQ=255)
                                                                                       ggATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattttaaa                                                      >  2:361786/1‑148 (MQ=255)
                                                                                       ggATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattttaaa                                                      <  1:3494439/148‑1 (MQ=255)
                                                                                       ggATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattttaaa                                                      >  1:275197/1‑148 (MQ=255)
                                                                                        gATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattttaaag                                                     >  1:2840507/1‑148 (MQ=255)
                                                                                         aTGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattttaaaga                                                    >  2:3008416/1‑148 (MQ=255)
                                                                                           gtgCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAtaaagattttaaagatt                                                  >  1:672663/1‑148 (MQ=255)
                                                                                                 cgcTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCt                                            <  1:1738053/148‑1 (MQ=255)
                                                                                                    tGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACg                                         <  2:1278464/148‑1 (MQ=255)
                                                                                                      tATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGcc                                       <  1:2021983/148‑1 (MQ=255)
                                                                                                      tATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGcc                                       >  1:3402702/1‑148 (MQ=255)
                                                                                                       aTTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCt                                      >  1:3263840/1‑148 (MQ=255)
                                                                                                        ttCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTg                                     >  2:908833/1‑148 (MQ=255)
                                                                                                        ttCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATAGTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTg                                     >  2:1178463/1‑148 (MQ=255)
                                                                                                         tCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGc                                    >  2:2741731/1‑148 (MQ=255)
                                                                                                         tCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGc                                    >  2:2419702/1‑148 (MQ=255)
                                                                                                         tCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGc                                    >  2:2118786/1‑148 (MQ=255)
                                                                                                         tCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACACTACGCCTGc                                    >  2:829032/1‑148 (MQ=255)
                                                                                                          cGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGct                                   <  1:1216983/148‑1 (MQ=255)
                                                                                                             tGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCtt                                <  1:3733512/148‑1 (MQ=255)
                                                                                                             tGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCtt                                <  1:120795/148‑1 (MQ=255)
                                                                                                                       gTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGc                      <  1:2546239/148‑1 (MQ=255)
                                                                                                                       gTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGc                      <  1:1319696/148‑1 (MQ=255)
                                                                                                                        tATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCt                     >  2:3029662/1‑148 (MQ=255)
                                                                                                                          tGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTtg                   <  2:542951/148‑1 (MQ=255)
                                                                                                                           gAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTtgt                  >  2:1640449/1‑148 (MQ=255)
                                                                                                                            aaTACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTtgtg                 <  1:2906907/148‑1 (MQ=255)
                                                                                                                            aaTACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTtgtg                 >  2:1242759/1‑148 (MQ=255)
                                                                                                                                 ttGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAAc            >  2:3566751/1‑148 (MQ=255)
                                                                                                                                 ttGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAAc            >  1:200896/1‑148 (MQ=255)
                                                                                                                                              |                                                                                                                                                
CGAAGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGG  >  CP009273/318882‑319168

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GACCGCGCGAAGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGT  >  CP009273/318875‑319173
                                                                                                                                                     |                                                                                                                                                     
GACCGCGCGAAGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATA                                                                                                                                                       >  SRR19973069.1200557/1‑150 (MQ=60)
GACCGCGCGAAGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATA                                                                                                                                                       <  SRR19973069.3483646/150‑1 (MQ=60)
 ACCGCGCGAAGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAG                                                                                                                                                      <  SRR19973069.3835700/150‑1 (MQ=60)
       CGAAGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATAT                                                                                                                                                >  SRR19973069.462410/1‑150 (MQ=60)
           GCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGC                                                                                                                                            <  SRR19973069.2596903/150‑1 (MQ=60)
           GCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGC                                                                                                                                            <  SRR19973069.804367/150‑1 (MQ=60)
             TGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGG                                                                                                                                          >  SRR19973069.409284/1‑150 (MQ=60)
              GGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGT                                                                                                                                         >  SRR19973069.1800391/1‑150 (MQ=60)
                 CACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCAT                                                                                                                                      >  SRR19973069.2079160/1‑150 (MQ=60)
                   CGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGG                                                                                                                                    <  SRR19973069.3562206/150‑1 (MQ=60)
                       GATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATAT                                                                                                                                <  SRR19973069.2671119/150‑1 (MQ=60)
                        ATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATG                                                                                                                               >  SRR19973069.2025820/1‑150 (MQ=60)
                          GGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGC                                                                                                                             <  SRR19973069.836742/150‑1 (MQ=60)
                           GTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCT                                                                                                                            <  SRR19973069.1864325/150‑1 (MQ=60)
                           GTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCT                                                                                                                            <  SRR19973069.1864363/150‑1 (MQ=60)
                              CTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTA                                                                                                                         >  SRR19973069.570960/1‑150 (MQ=60)
                                GAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATT                                                                                                                       >  SRR19973069.3789621/1‑150 (MQ=60)
                                GAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATT                                                                                                                       <  SRR19973069.3272093/150‑1 (MQ=60)
                                 AAGAGTTTCATCTGGTTATTGTCGATAACGGGAGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTT                                                                                                                      <  SRR19973069.1402362/150‑1 (MQ=60)
                                    AGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTTGCGGTCATGGATATGGCTCTATTTATC                                                                                                                   >  SRR19973069.2394835/1‑150 (MQ=60)
                                    AGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATC                                                                                                                   <  SRR19973069.1671719/150‑1 (MQ=60)
                                    AGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATC                                                                                                                   <  SRR19973069.273116/150‑1 (MQ=60)
                                     GTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCC                                                                                                                  <  SRR19973069.2168274/150‑1 (MQ=60)
                                      TTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCA                                                                                                                 >  SRR19973069.3288333/1‑150 (MQ=60)
                                         CATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGG                                                                                                              <  SRR19973069.1292625/150‑1 (MQ=60)
                                          ATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGAC                                                                                                             >  SRR19973069.2176364/1‑150 (MQ=60)
                                           TCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCC                                                                                                            <  SRR19973069.2603854/150‑1 (MQ=60)
                                            CTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCA                                                                                                           <  SRR19973069.1765426/150‑1 (MQ=60)
                                            CTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCA                                                                                                           >  SRR19973069.2857109/1‑150 (MQ=60)
                                               GTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATT                                                                                                        <  SRR19973069.2890560/150‑1 (MQ=60)
                                                 TATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGG                                                                                                      <  SRR19973069.1918194/150‑1 (MQ=60)
                                                   TTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTG                                                                                                    <  SRR19973069.2951682/150‑1 (MQ=60)
                                                     GTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCG                                                                                                  >  SRR19973069.833244/1‑150 (MQ=60)
                                                       CGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGT                                                                                                >  SRR19973069.1287035/1‑150 (MQ=60)
                                                        GATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTG                                                                                               <  SRR19973069.2660238/150‑1 (MQ=60)
                                                              GGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCT                                                                                         >  SRR19973069.400606/1‑150 (MQ=60)
                                                              GGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCT                                                                                         >  SRR19973069.63411/1‑150 (MQ=60)
                                                              GGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCT                                                                                         >  SRR19973069.63431/1‑150 (MQ=60)
                                                                GCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCC                                                                                       <  SRR19973069.1321410/150‑1 (MQ=60)
                                                                       GAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTT                                                                                <  SRR19973069.998549/150‑1 (MQ=60)
                                                                        AGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTG                                                                               <  SRR19973069.701363/150‑1 (MQ=60)
                                                                         GGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGG                                                                              >  SRR19973069.2817853/1‑150 (MQ=60)
                                                                         GGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGAGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGT                                                                              >  SRR19973069.2817831/1‑150 (MQ=60)
                                                                              TGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCT                                                                         >  SRR19973069.3297848/1‑150 (MQ=60)
                                                                              TGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCT                                                                         >  SRR19973069.879054/1‑150 (MQ=60)
                                                                                GCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTAT                                                                       >  SRR19973069.844960/1‑150 (MQ=60)
                                                                                 CCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATA                                                                      >  SRR19973069.3059728/1‑150 (MQ=60)
                                                                                  CTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAA                                                                     >  SRR19973069.36064/1‑150 (MQ=60)
                                                                                    CTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAG                                                                   <  SRR19973069.3783840/150‑1 (MQ=60)
                                                                                    CTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAG                                                                   <  SRR19973069.3236253/150‑1 (MQ=60)
                                                                                      GAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGAT                                                                 >  SRR19973069.41544/1‑150 (MQ=60)
                                                                                      GAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGAT                                                                 <  SRR19973069.402397/150‑1 (MQ=60)
                                                                                       AATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATT                                                                >  SRR19973069.3024616/1‑150 (MQ=60)
                                                                                       AATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATT                                                                >  SRR19973069.2879603/1‑150 (MQ=60)
                                                                                       AATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATT                                                                >  SRR19973069.2879616/1‑150 (MQ=60)
                                                                                            CGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAA                                                           >  SRR19973069.277135/1‑150 (MQ=60)
                                                                                             GGGGTGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAG                                                          >  SRR19973069.2859004/1‑150 (MQ=60)
                                                                                             GGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAG                                                          >  SRR19973069.2859245/1‑150 (MQ=60)
                                                                                              GGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGA                                                         <  SRR19973069.3516960/150‑1 (MQ=60)
                                                                                                ATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATT                                                       >  SRR19973069.677294/1‑150 (MQ=60)
                                                                                                 TGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTT                                                      >  SRR19973069.930878/1‑150 (MQ=60)
                                                                                                  GTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTA                                                     <  SRR19973069.3151829/150‑1 (MQ=60)
                                                                                                      TGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCT                                                 <  SRR19973069.2058450/150‑1 (MQ=60)
                                                                                                        CGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTAC                                               <  SRR19973069.1750250/150‑1 (MQ=60)
                                                                                                           TGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCC                                            >  SRR19973069.3425237/1‑150 (MQ=60)
                                                                                                           TGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCC                                            >  SRR19973069.3424898/1‑150 (MQ=60)
                                                                                                            GTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCT                                           >  SRR19973069.3285221/1‑150 (MQ=60)
                                                                                                            GTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCT                                           <  SRR19973069.448890/150‑1 (MQ=60)
                                                                                                             TATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTG                                          <  SRR19973069.2036033/150‑1 (MQ=60)
                                                                                                               TTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCAAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACACTACGCCTGCT                                        >  SRR19973069.2428051/1‑150 (MQ=60)
                                                                                                                 CGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCT                                      <  SRR19973069.1225317/150‑1 (MQ=60)
                                                                                                                    TGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTA                                   <  SRR19973069.121968/150‑1 (MQ=60)
                                                                                                                    TGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTA                                   <  SRR19973069.121945/150‑1 (MQ=60)
                                                                                                                    TGCGGGGCTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTA                                   <  SRR19973069.3757851/150‑1 (MQ=60)
                                                                                                                           CTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAG                            >  SRR19973069.516899/1‑150 (MQ=60)
                                                                                                                           CTTGTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAG                            <  SRR19973069.2776160/150‑1 (MQ=60)
                                                                                                                              GTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTT                         <  SRR19973069.1328753/150‑1 (MQ=60)
                                                                                                                              GTATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTT                         <  SRR19973069.2563071/150‑1 (MQ=60)
                                                                                                                                ATGAATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGT                       <  SRR19973069.3617646/150‑1 (MQ=60)
                                                                                                                                   AATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGAC                    <  SRR19973069.2926127/150‑1 (MQ=60)
                                                                                                                                   AATACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGAC                    <  SRR19973069.2926284/150‑1 (MQ=60)
                                                                                                                                      ACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAAC                 >  SRR19973069.1374483/1‑150 (MQ=60)
                                                                                                                                      ACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAAC                 >  SRR19973069.3287929/1‑150 (MQ=60)
                                                                                                                                      ACTTGTCCGGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAAC                 >  SRR19973069.202501/1‑150 (MQ=60)
                                                                                                                                              GGCATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCC         <  SRR19973069.579475/150‑1 (MQ=60)
                                                                                                                                                CATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGG       >  SRR19973069.2141213/1‑150 (MQ=60)
                                                                                                                                                 ATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGT      >  SRR19973069.943316/1‑150 (MQ=60)
                                                                                                                                                 ATATAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGACCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGT      >  SRR19973069.39398/1‑150 (MQ=60)
                                                                                                                                                    TAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCCGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCG   >  SRR19973069.3709424/1‑150 (MQ=60)
                                                                                                                                                    TAGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCG   >  SRR19973069.993914/1‑150 (MQ=60)
                                                                                                                                                     AGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGT  >  SRR19973069.969298/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                                     
GACCGCGCGAAGCTGGGCACGTTGATGGTCCTGAAGAGTTTCATCTGGTTATTGTCGATAACGGGCGTTCTGAGGTGCTGGCCTCTGAATTTCGGGATGTGCTGCGCTGTATTCGCTGCGGGGCTTGTATGAATACTTGTCCGGCATATCGCCATATTGGCGGTCATGGATATGGCTCTATTTATCCAGGGCCAATTGGTGCGGTGATTTCTCCGCTACTTGGCGGCTATAAAGATTTTAAAGATTTACCCTACGCCTGCTCTTTATGCACAGCTTGTGACAACGTGTGTCCGGTGCGT  >  CP009273/318875‑319173

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑