Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 230,579 C→T 100% E231K (GAA→AAA)  gloB ← hydroxyacylglutathione hydrolase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP009273230,5790CT98.6% 793.7 / 0.1 221E231K (GAA→AAA) gloBhydroxyacylglutathione hydrolase
Reads supporting (aligned to +/- strand):  ref base C (1/2);  new base T (91/127);  total (92/129)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.96e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.
Rejected as polymorphism: Polymorphic base substitution creates a homopolymer stretch.

Only 100 of 227 total aligned reads displayed.

TTTGCCTTCATGTGTGTGTCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTCTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAAT  >  CP009273/230432‑230725
                                                                                                                                                   |                                                                                                                                                   
tttGCCTTCATGTGTGTGTCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGtttttt                                                                                                                                                   >  1:548497/1‑150 (MQ=255)
 ttGCCTTCATGTGTGTGTCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTtttttc                                                                                                                                                  <  2:4047077/150‑1 (MQ=255)
    ccTTCATGTGTGTGTCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCCTGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTcatt                                                                                                                                               <  2:2164311/150‑1 (MQ=255)
    ccTTCATGTGTGTGTCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTcatt                                                                                                                                               <  2:3559829/150‑1 (MQ=255)
    ccTTCATGTGTGTGTCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTcatt                                                                                                                                               <  1:2177311/150‑1 (MQ=255)
      ttCATGTGTGTGTCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTcattaa                                                                                                                                             <  1:773770/150‑1 (MQ=255)
        cATGTGTGTGTCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTcattaatt                                                                                                                                           <  1:3675208/150‑1 (MQ=255)
                 gtCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTcattaattacattaatt                                                                                                                                  >  2:3281248/1‑150 (MQ=255)
                 gtCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTcattaattacattaatt                                                                                                                                  <  1:3617364/150‑1 (MQ=255)
                 gtCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTcattaattacattaatt                                                                                                                                  >  2:3281223/1‑150 (MQ=255)
                  tCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTcattaattacattaatta                                                                                                                                 <  1:344714/150‑1 (MQ=255)
                    aaTAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTaaa                                                                                                                               <  2:2626146/150‑1 (MQ=255)
                      tAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATc                                                                                                                             >  2:3391125/1‑150 (MQ=255)
                       aGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCa                                                                                                                            <  2:2908655/150‑1 (MQ=255)
                        gTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCaa                                                                                                                           >  1:2780225/1‑150 (MQ=255)
                         ttGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAat                                                                                                                          >  1:3816195/1‑150 (MQ=255)
                           gCTTAAAAGACGACCGATCATACCGGCGAACGGAGCAGATGACACGAAATTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGATTTTTCATTAATTACATTAATTAAATCAatat                                                                                                                        <  1:1696932/150‑1 (MQ=255)
                               aaaaGACGACCGATCATAACGGCGATCGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTc                                                                                                                    <  2:1880457/150‑1 (MQ=255)
                               aaaaGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTc                                                                                                                    >  1:2965220/1‑150 (MQ=255)
                               aaaaGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTc                                                                                                                    <  2:932246/150‑1 (MQ=255)
                               aaaaGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTAACTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTc                                                                                                                    <  2:1368848/150‑1 (MQ=255)
                                aaaGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTcc                                                                                                                   <  2:2217293/150‑1 (MQ=255)
                                aaaGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTcc                                                                                                                   <  1:2207168/150‑1 (MQ=255)
                                   gacgacCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTATATCAATATCTTCCGtt                                                                                                                <  2:1181179/150‑1 (MQ=255)
                                   gacgacCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGtt                                                                                                                <  1:1637363/150‑1 (MQ=255)
                                    acgacCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTc                                                                                                               >  1:1469645/1‑150 (MQ=255)
                                    acgacCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTc                                                                                                               <  2:810445/150‑1 (MQ=255)
                                    acgacCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTc                                                                                                               <  2:2073608/150‑1 (MQ=255)
                                     cgacCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCt                                                                                                              <  2:2735470/150‑1 (MQ=255)
                                     cgacCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCt                                                                                                              <  1:789591/150‑1 (MQ=255)
                                     cgacCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCAGTTCt                                                                                                              <  1:1541377/150‑1 (MQ=255)
                                      gacCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCtt                                                                                                             <  1:3436458/150‑1 (MQ=255)
                                       acCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGCCCTTACCCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑a                                                                                                           <  2:228590/150‑1 (MQ=255)
                                       acCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑a                                                                                                           <  2:228726/150‑1 (MQ=255)
                                       acCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑a                                                                                                           >  2:472391/1‑150 (MQ=255)
                                          gATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑aaaa                                                                                                        >  2:1124030/1‑150 (MQ=255)
                                          gATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑aaaa                                                                                                        >  1:3134569/1‑150 (MQ=255)
                                           aTCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑aaaaa                                                                                                       >  2:2120/1‑150 (MQ=255)
                                                aaCGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACAt                                                                                                  >  1:1246236/1‑150 (MQ=255)
                                                aaCGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACAt                                                                                                  <  2:322899/150‑1 (MQ=255)
                                                aaCGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACAt                                                                                                  >  1:1002586/1‑150 (MQ=255)
                                                aaCGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACAt                                                                                                  >  1:1400280/1‑150 (MQ=255)
                                                 aCGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACAtt                                                                                                 <  1:3872095/150‑1 (MQ=255)
                                                    gCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAAt                                                                                              >  2:498187/1‑150 (MQ=255)
                                                     cGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTGAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAAtt                                                                                             <  2:1095626/150‑1 (MQ=255)
                                                      gAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAAttt                                                                                            <  2:3844848/150‑1 (MQ=255)
                                                          ggAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCAGTTCTT‑AAAAAAACATTAATTTGCCg                                                                                        >  1:3179736/1‑150 (MQ=255)
                                                            aGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCt                                                                                      <  2:1534639/150‑1 (MQ=255)
                                                            aGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTACGTTCTT‑AAAAAAACATTAATTTGCCGCt                                                                                      >  1:3372698/1‑150 (MQ=255)
                                                             gCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCCTGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTTAAAAAAAACATTAATTTGCCGCt                                                                                      <  1:1178864/150‑1 (MQ=255)
                                                              ccGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCa                                                                                    >  2:56842/1‑150 (MQ=255)
                                                               cGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCAt                                                                                   <  1:1491366/150‑1 (MQ=255)
                                                                   gACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCAttttt                                                                               >  2:2020912/1‑150 (MQ=255)
                                                                     cAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCa                                                                             >  1:3558749/1‑150 (MQ=255)
                                                                     cAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCa                                                                             >  1:157593/1‑150 (MQ=255)
                                                                     cAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCa                                                                             >  1:1641163/1‑150 (MQ=255)
                                                                       aGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGa                                                                           <  1:895247/150‑1 (MQ=255)
                                                                       aGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGa                                                                           >  1:1814633/1‑150 (MQ=255)
                                                                        gAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGaa                                                                          >  2:1036827/1‑150 (MQ=255)
                                                                          aaGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAAtt                                                                        <  2:2944364/150‑1 (MQ=255)
                                                                          aaGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAAtt                                                                        >  2:2605168/1‑150 (MQ=255)
                                                                           aGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTa                                                                       >  2:3718889/1‑150 (MQ=255)
                                                                            gTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTAc                                                                      >  2:3474209/1‑150 (MQ=255)
                                                                             ttttATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACg                                                                     <  2:2349562/150‑1 (MQ=255)
                                                                                tATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGt                                                                  <  1:1680397/150‑1 (MQ=255)
                                                                                 aTCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTa                                                                 >  1:4085476/1‑150 (MQ=255)
                                                                                    aGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAgtg                                                              >  1:99030/1‑150 (MQ=255)
                                                                                       aCCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTa                                                           <  1:2929994/150‑1 (MQ=255)
                                                                                          tATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTCTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTAttt                                                        <  2:267147/150‑1 (MQ=255)
                                                                                             ctttctttGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGAt                                                     >  1:2651373/1‑150 (MQ=255)
                                                                                              tttctttGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAATCATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGAtt                                                    >  1:1937894/1‑150 (MQ=255)
                                                                                              tttctttGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGAtt                                                    <  2:283759/150‑1 (MQ=255)
                                                                                                tctttGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGAtttt                                                  >  1:549624/1‑150 (MQ=255)
                                                                                                tctttGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGAtttt                                                  <  1:1241590/150‑1 (MQ=255)
                                                                                                   ttGACCTTACCCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTg                                               <  2:2712627/150‑1 (MQ=255)
                                                                                                   ttGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTg                                               >  1:2391692/1‑150 (MQ=255)
                                                                                                    tGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGc                                              >  1:1523729/1‑150 (MQ=255)
                                                                                                     gACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGcc                                             <  1:1484177/150‑1 (MQ=255)
                                                                                                     gACCTTAACCATGCAAAACGCTCTTCAGGTAGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCATAATTAAGGGGAGTGTTATTTGATTTTTTGcc                                             >  1:4005007/1‑150 (MQ=255)
                                                                                                      aCCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGccc                                            <  1:3209200/150‑1 (MQ=255)
                                                                                                      aCCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGccc                                            <  1:1293855/150‑1 (MQ=255)
                                                                                                      aCCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGccc                                            <  1:1293909/150‑1 (MQ=255)
                                                                                                       ccTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCg                                           <  2:260293/150‑1 (MQ=255)
                                                                                                        cTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGt                                          <  1:3331572/150‑1 (MQ=255)
                                                                                                         ttAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTa                                         <  2:1519035/150‑1 (MQ=255)
                                                                                                              cATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTcc                                    >  1:1121097/1‑150 (MQ=255)
                                                                                                                tGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCtt                                  <  2:3488992/150‑1 (MQ=255)
                                                                                                                 gCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTa                                 <  2:279691/150‑1 (MQ=255)
                                                                                                                     aaCGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACtt                             >  2:730633/1‑150 (MQ=255)
                                                                                                                     aaCGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACtt                             >  1:102023/1‑150 (MQ=255)
                                                                                                                       cGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACTTTa                           <  2:3230901/150‑1 (MQ=255)
                                                                                                                        gCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACTTTAc                          <  1:2015069/150‑1 (MQ=255)
                                                                                                                           ctTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCGATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGat                       <  1:84073/150‑1 (MQ=255)
                                                                                                                             tCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGataa                     >  2:2304372/1‑150 (MQ=255)
                                                                                                                              cAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGataat                    <  2:1749784/150‑1 (MQ=255)
                                                                                                                                 gttgttGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATaataat                 >  2:3668303/1‑150 (MQ=255)
                                                                                                                                     ttgCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCAtt             >  2:2246833/1‑150 (MQ=255)
                                                                                                                                          ataatGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATgg        >  1:2090805/1‑150 (MQ=255)
                                                                                                                                              tGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACaa    <  1:1856226/150‑1 (MQ=255)
                                                                                                                                                ttttttCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAAt  <  2:2894886/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                   
TTTGCCTTCATGTGTGTGTCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTCTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAAT  >  CP009273/230432‑230725

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TTTTGCCTTCATGTGTGTGTCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTCTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATC  >  CP009273/230431‑230726
                                                                                                                                                    |                                                                                                                                                    
TTTTGCCTTCATGTGTGTGTCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTT                                                                                                                                                     <  SRR19973074.1312649/150‑1 (MQ=60)
 TTTGCCTTCATGTGTGTGTCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTT                                                                                                                                                    >  SRR19973074.549582/1‑150 (MQ=60)
     CCTTCATGTGTGTGTCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATT                                                                                                                                                <  SRR19973074.2181399/150‑1 (MQ=60)
      CTTCATGTGTGTGTCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTA                                                                                                                                               <  SRR19973074.1678914/150‑1 (MQ=60)
       TTCATGTGTGTGTCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAA                                                                                                                                              <  SRR19973074.775256/150‑1 (MQ=60)
         CATGTGTGTGTCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATT                                                                                                                                            <  SRR19973074.3682004/150‑1 (MQ=60)
            GTGTGTGTCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACA                                                                                                                                         <  SRR19973074.2939629/150‑1 (MQ=60)
             TGTGTGTCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACAT                                                                                                                                        <  SRR19973074.1872317/150‑1 (MQ=60)
                  GTCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATT                                                                                                                                   <  SRR19973074.3624048/150‑1 (MQ=60)
                   TCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACTGAGCAGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCTATAATGTTTTTTCATTAATTACATTAATTA                                                                                                                                  <  SRR19973074.345349/150‑1 (MQ=60)
                   TCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTA                                                                                                                                  >  SRR19973074.2969282/1‑150 (MQ=60)
                   TCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTA                                                                                                                                  <  SRR19973074.345338/150‑1 (MQ=60)
                   TCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTA                                                                                                                                  <  SRR19973074.775554/150‑1 (MQ=60)
                         GTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAA                                                                                                                            >  SRR19973074.2785326/1‑150 (MQ=60)
                         GTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAA                                                                                                                            <  SRR19973074.2165346/150‑1 (MQ=60)
                          TTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAAT                                                                                                                           >  SRR19973074.3823161/1‑150 (MQ=60)
                          TTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAAT                                                                                                                           <  SRR19973074.3822848/150‑1 (MQ=60)
                            GCTTAAAAGACGACCGATCATACCGGCGAACGGAGCAGATGACACGAAATTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGATTTTTCATTAATTACATTAATTAAATCAATAT                                                                                                                         <  SRR19973074.1700102/150‑1 (MQ=60)
                            GCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATAT                                                                                                                         >  SRR19973074.3158963/1‑150 (MQ=60)
                            GCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATAT                                                                                                                         <  SRR19973074.1699662/150‑1 (MQ=60)
                                AAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTC                                                                                                                     >  SRR19973074.3882187/1‑150 (MQ=60)
                                AAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTC                                                                                                                     >  SRR19973074.2970730/1‑150 (MQ=60)
                                 AAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCC                                                                                                                    <  SRR19973074.127035/150‑1 (MQ=60)
                                 AAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCC                                                                                                                    <  SRR19973074.2211317/150‑1 (MQ=60)
                                  AAGACGACCGCTCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCCTGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCG                                                                                                                   <  SRR19973074.1599861/150‑1 (MQ=60)
                                    GACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTT                                                                                                                 <  SRR19973074.1640891/150‑1 (MQ=60)
                                    GACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTT                                                                                                                 <  SRR19973074.1640468/150‑1 (MQ=60)
                                     ACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTC                                                                                                                >  SRR19973074.1472401/1‑150 (MQ=60)
                                     ACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTC                                                                                                                >  SRR19973074.1472407/1‑150 (MQ=60)
                                      CGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCT                                                                                                               <  SRR19973074.791108/150‑1 (MQ=60)
                                      CGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCT                                                                                                               <  SRR19973074.791274/150‑1 (MQ=60)
                                      CGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCT                                                                                                               <  SRR19973074.133058/150‑1 (MQ=60)
                                      CGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCAGTTCT                                                                                                               <  SRR19973074.1544301/150‑1 (MQ=60)
                                      CGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCAGTTCT                                                                                                               <  SRR19973074.3647215/150‑1 (MQ=60)
                                       GACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT                                                                                                              <  SRR19973074.3442789/150‑1 (MQ=60)
                                       GACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT                                                                                                              <  SRR19973074.3442837/150‑1 (MQ=60)
                                           GATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAA                                                                                                         >  SRR19973074.3140314/1‑150 (MQ=60)
                                           GATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAA                                                                                                         >  SRR19973074.1528486/1‑150 (MQ=60)
                                                 AACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACAT                                                                                                   >  SRR19973074.1402852/1‑150 (MQ=60)
                                                 AACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACAT                                                                                                   >  SRR19973074.1248624/1‑150 (MQ=60)
                                                 AACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACAT                                                                                                   >  SRR19973074.1004448/1‑150 (MQ=60)
                                                  ACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATT                                                                                                  <  SRR19973074.3879177/150‑1 (MQ=60)
                                                  ACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATT                                                                                                  >  SRR19973074.960924/1‑150 (MQ=60)
                                                    GGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCATAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAA                                                                                                <  SRR19973074.838822/150‑1 (MQ=60)
                                                     GCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAAT                                                                                               <  SRR19973074.3127328/150‑1 (MQ=60)
                                                     GCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAAT                                                                                               <  SRR19973074.1062097/150‑1 (MQ=60)
                                                       GAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTT                                                                                             >  SRR19973074.1559059/1‑150 (MQ=60)
                                                           GGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCAGTTCTT‑AAAAAAACATTAATTTGCCG                                                                                         >  SRR19973074.3185591/1‑150 (MQ=60)
                                                             AGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTACGTTCTT‑AAAAAAACATTAATTTGCCGCT                                                                                       >  SRR19973074.3378949/1‑150 (MQ=60)
                                                              GCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCCTGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTTAAAAAAAACATTAATTTGCCGCT                                                                                       <  SRR19973074.1181109/150‑1 (MQ=60)
                                                                CGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATTTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCAT                                                                                    <  SRR19973074.1494083/150‑1 (MQ=60)
                                                                CGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCAT                                                                                    >  SRR19973074.2205087/1‑150 (MQ=60)
                                                                CGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCAT                                                                                    <  SRR19973074.1494175/150‑1 (MQ=60)
                                                                CGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCAT                                                                                    <  SRR19973074.1350743/150‑1 (MQ=60)
                                                                 GATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATT                                                                                   >  SRR19973074.2672995/1‑150 (MQ=60)
                                                                   TGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTT                                                                                 >  SRR19973074.3348185/1‑150 (MQ=60)
                                                                      CAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCA                                                                              >  SRR19973074.157948/1‑150 (MQ=60)
                                                                      CAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCA                                                                              >  SRR19973074.3565307/1‑150 (MQ=60)
                                                                      CAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCA                                                                              >  SRR19973074.1644268/1‑150 (MQ=60)
                                                                        AGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGA                                                                            >  SRR19973074.1818088/1‑150 (MQ=60)
                                                                        AGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGA                                                                            >  SRR19973074.1818067/1‑150 (MQ=60)
                                                                        AGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGA                                                                            >  SRR19973074.2332125/1‑150 (MQ=60)
                                                                        AGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGA                                                                            >  SRR19973074.2332162/1‑150 (MQ=60)
                                                                        AGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGA                                                                            <  SRR19973074.858024/150‑1 (MQ=60)
                                                                        AGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGA                                                                            <  SRR19973074.896957/150‑1 (MQ=60)
                                                                        AGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGA                                                                            <  SRR19973074.4063571/150‑1 (MQ=60)
                                                                                 TATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGT                                                                   <  SRR19973074.1683545/150‑1 (MQ=60)
                                                                                  ATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTA                                                                  >  SRR19973074.4093036/1‑150 (MQ=60)
                                                                                  ATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTA                                                                  >  SRR19973074.4093037/1‑150 (MQ=60)
                                                                                     AGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTG                                                               >  SRR19973074.99274/1‑150 (MQ=60)
                                                                                        ACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTA                                                            <  SRR19973074.2935440/150‑1 (MQ=60)
                                                                                         CCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTAT                                                           >  SRR19973074.2004820/1‑150 (MQ=60)
                                                                                         CCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTAT                                                           >  SRR19973074.2082082/1‑150 (MQ=60)
                                                                                          CTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATT                                                          <  SRR19973074.2832549/150‑1 (MQ=60)
                                                                                              CTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGAT                                                      >  SRR19973074.2656318/1‑150 (MQ=60)
                                                                                               TTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAATCATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATT                                                     >  SRR19973074.1941599/1‑150 (MQ=60)
                                                                                                 TCTTTGACCTTAACCCTGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTT                                                   <  SRR19973074.1244125/150‑1 (MQ=60)
                                                                                                 TCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTT                                                   <  SRR19973074.1243969/150‑1 (MQ=60)
                                                                                                 TCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTT                                                   >  SRR19973074.550710/1‑150 (MQ=60)
                                                                                                 TCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTT                                                   >  SRR19973074.550551/1‑150 (MQ=60)
                                                                                                    TTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTG                                                <  SRR19973074.1208901/150‑1 (MQ=60)
                                                                                                    TTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTG                                                >  SRR19973074.2396129/1‑150 (MQ=60)
                                                                                                     TGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGC                                               >  SRR19973074.1526605/1‑150 (MQ=60)
                                                                                                      GACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCC                                              <  SRR19973074.1486972/150‑1 (MQ=60)
                                                                                                      GACCTTAACCATGCAAAACGCTCTTCAGGTAGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCATAATTAAGGGGAGTGTTATTTGATTTTTTGCC                                              >  SRR19973074.4012411/1‑150 (MQ=60)
                                                                                                       ACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCC                                             <  SRR19973074.3215115/150‑1 (MQ=60)
                                                                                                       ACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCC                                             <  SRR19973074.1296308/150‑1 (MQ=60)
                                                                                                       ACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCC                                             <  SRR19973074.1296309/150‑1 (MQ=60)
                                                                                                       ACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCC                                             <  SRR19973074.1296362/150‑1 (MQ=60)
                                                                                                         CTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGT                                           >  SRR19973074.119482/1‑150 (MQ=60)
                                                                                                         CTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGT                                           <  SRR19973074.3337754/150‑1 (MQ=60)
                                                                                                         CTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGT                                           >  SRR19973074.4096818/1‑150 (MQ=60)
                                                                                                            AACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTAAC                                        <  SRR19973074.3470130/150‑1 (MQ=60)
                                                                                                               CATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCC                                     >  SRR19973074.1123213/1‑150 (MQ=60)
                                                                                                                 TGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTCTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTT                                   >  SRR19973074.1750473/1‑150 (MQ=60)
                                                                                                                     AAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACT                               <  SRR19973074.3617709/150‑1 (MQ=60)
                                                                                                                     AAACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACT                               >  SRR19973074.3307272/1‑150 (MQ=60)
                                                                                                                      AACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACTT                              >  SRR19973074.102273/1‑150 (MQ=60)
                                                                                                                      AACGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAAGTACATTAATTAAATCAATATGTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAAGTACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACGT                              >  SRR19973074.102268/1‑150 (MQ=60)
                                                                                                                        CGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACTTTA                            >  SRR19973074.1474131/1‑150 (MQ=60)
                                                                                                                        CGCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACTTTA                            <  SRR19973074.2534987/150‑1 (MQ=60)
                                                                                                                         GCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACTTTAC                           <  SRR19973074.2018888/150‑1 (MQ=60)
                                                                                                                         GCTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACTTTAC                           <  SRR19973074.3765204/150‑1 (MQ=60)
                                                                                                                          CTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACG                          >  SRR19973074.1166204/1‑150 (MQ=60)
                                                                                                                          CTCTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACG                          >  SRR19973074.3256543/1‑150 (MQ=60)
                                                                                                                            CTTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCGATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGAT                        <  SRR19973074.84275/150‑1 (MQ=60)
                                                                                                                             TTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATA                       <  SRR19973074.18322/150‑1 (MQ=60)
                                                                                                                             TTCAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATA                       <  SRR19973074.2081194/150‑1 (MQ=60)
                                                                                                                               CAGGTTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAAT                     <  SRR19973074.1621824/150‑1 (MQ=60)
                                                                                                                                   TTGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATC                 >  SRR19973074.3871466/1‑150 (MQ=60)
                                                                                                                                    TGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCA                <  SRR19973074.3200899/150‑1 (MQ=60)
                                                                                                                                    TGTTGCAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCA                <  SRR19973074.338769/150‑1 (MQ=60)
                                                                                                                                         CAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTAT           >  SRR19973074.1512176/1‑150 (MQ=60)
                                                                                                                                         CAATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTAT           >  SRR19973074.1512223/1‑150 (MQ=60)
                                                                                                                                           ATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGG         <  SRR19973074.720198/150‑1 (MQ=60)
                                                                                                                                           ATAATGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGG         >  SRR19973074.2094720/1‑150 (MQ=60)
                                                                                                                                               TGTTTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAA     <  SRR19973074.1859770/150‑1 (MQ=60)
                                                                                                                                                  TTTTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATC  >  SRR19973074.3824769/1‑150 (MQ=60)
                                                                                                                                                    |                                                                                                                                                    
TTTTGCCTTCATGTGTGTGTCAATAGTTGCTTAAAAGACGACCGATCATAACGGCGAACGGAGCCGATGACAAGAAAGTTTTATCAGAACCTATCTTTCTTTGACCTTAACCATGCAAAACGCTCTTCAGGTTGTTGCAATAATGTTTCTTCATTAATTACATTAATTAAATCAATATCTTCCGTTCTT‑AAAAAAACATTAATTTGCCGCTCATTTTTCAGAATTACGGGTAGTGTTATTTGATTTTTTGCCCGTAACTCCTTAACTTTACGATAATAATCATTTATGGACAAATC  >  CP009273/230431‑230726

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑