Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F98 I0 R1 799 294.1 9752082 92.2% 8991419 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,563,276 G→T 66.1% H491N (CAC→AAC)  gadC ← glutamate:gamma‑aminobutyric acid antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,563,2760GT66.1% 234.2 / 277.5 230H491N (CAC→AAC) gadCglutamate:gamma‑aminobutyric acid antiporter
Reads supporting (aligned to +/- strand):  ref base G (53/25);  new base T (80/72);  total (133/97)
Fisher's exact test for biased strand distribution p-value = 3.41e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.88e-01

Only 100 of 230 total aligned reads displayed.

CCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATATC  >  CP009273/1563129‑1563420
                                                                                                                                                   |                                                                                                                                                
cccGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGAc                                                                                                                                                >  1:2343375/1‑150 (MQ=255)
cccGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGAc                                                                                                                                                >  1:2409015/1‑150 (MQ=255)
 ccGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTAcc                                                                                                                                               <  1:4008740/150‑1 (MQ=255)
   gTTAAGTCAATACGACAGCAAGCACGAAACAGGGAGCGATGAATTATCGCTCCCTTGACTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCtt                                                                                                                                             <  1:2584188/150‑1 (MQ=255)
   gTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCtt                                                                                                                                             <  2:1114192/150‑1 (MQ=255)
    ttAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCttt                                                                                                                                            <  2:2787632/150‑1 (MQ=255)
     tAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCtttt                                                                                                                                           >  2:3948736/1‑150 (MQ=255)
     tAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCtttt                                                                                                                                           >  2:3843422/1‑150 (MQ=255)
      aaGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGACCGTGCACGCGGGTGCAGGAAGAAGTTACCTGTTg                                                                                                                                          >  2:1013663/1‑150 (MQ=255)
      aaGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTg                                                                                                                                          >  2:1514188/1‑150 (MQ=255)
       aGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTgg                                                                                                                                         >  2:3732491/1‑150 (MQ=255)
           aaTACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCg                                                                                                                                     >  2:571421/1‑150 (MQ=255)
             tACGACAGCAAGCACGAAAAAGGGAGCGATGACTTATCGCTCCCTTGTCTTATACCCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGtt                                                                                                                                   <  2:1307406/150‑1 (MQ=255)
              aCGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTc                                                                                                                                  <  1:2665661/150‑1 (MQ=255)
               cGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCt                                                                                                                                 <  2:4726007/150‑1 (MQ=255)
                gACAGCAAGCACGAAAGAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTg                                                                                                                                <  2:4523162/150‑1 (MQ=255)
                gACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTg                                                                                                                                <  1:2121239/150‑1 (MQ=255)
                gACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTg                                                                                                                                >  2:3443385/1‑150 (MQ=255)
                       agcaCGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTg                                                                                                                         >  1:3813781/1‑150 (MQ=255)
                       agcaCGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTg                                                                                                                         <  2:1963076/150‑1 (MQ=255)
                       agcaCGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTg                                                                                                                         <  2:3391896/150‑1 (MQ=255)
                        gcaCGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGa                                                                                                                        <  1:2804896/150‑1 (MQ=255)
                             aaaatGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATAGAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTGTGGTGCGTTCTGACTGGTGATTGGc                                                                                                                   >  2:2321529/1‑150 (MQ=255)
                             aaaaaGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGc                                                                                                                   >  2:1198816/1‑150 (MQ=255)
                               aaaGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTc                                                                                                                 >  1:1779716/1‑150 (MQ=255)
                                aaGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATCGTGTGGTGAACGTGCCCGCGGGTGCAGGAAGAAGTGACCTGTTGGTGCGTTCTGACTGTTGATTGGCTcc                                                                                                                >  2:4105782/1‑150 (MQ=255)
                                aaGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTcc                                                                                                                <  2:3434723/150‑1 (MQ=255)
                                     aGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGt                                                                                                           <  2:1710810/150‑1 (MQ=255)
                                     aGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGt                                                                                                           <  2:2299170/150‑1 (MQ=255)
                                       cGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGa                                                                                                         <  1:3614663/150‑1 (MQ=255)
                                        gATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGAc                                                                                                        >  2:2490449/1‑150 (MQ=255)
                                         aTGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACg                                                                                                       >  1:763653/1‑150 (MQ=255)
                                         aTGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACg                                                                                                       >  1:1254276/1‑150 (MQ=255)
                                            aaTTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCg                                                                                                    >  1:394704/1‑150 (MQ=255)
                                            aaTTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCg                                                                                                    >  1:130371/1‑150 (MQ=255)
                                             aTTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCgg                                                                                                   >  1:1473475/1‑150 (MQ=255)
                                              ttATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGt                                                                                                  >  2:494495/1‑150 (MQ=255)
                                              ttATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGt                                                                                                  >  1:3057702/1‑150 (MQ=255)
                                               tATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTa                                                                                                 >  2:3504557/1‑150 (MQ=255)
                                                 tCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTAtt                                                                                               >  2:2079639/1‑150 (MQ=255)
                                                   gCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTAtttg                                                                                             <  2:1365187/150‑1 (MQ=255)
                                                    cTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTAtttgc                                                                                            >  2:3344868/1‑150 (MQ=255)
                                                    cTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTAtttgc                                                                                            >  2:2585369/1‑150 (MQ=255)
                                                     tCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTAtttgct                                                                                           <  1:3508580/150‑1 (MQ=255)
                                                      cccTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTAtttgctt                                                                                          >  2:424912/1‑150 (MQ=255)
                                                        cTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTAtttgctttg                                                                                        >  2:4758126/1‑150 (MQ=255)
                                                          tGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGcc                                                                                      >  1:2688313/1‑150 (MQ=255)
                                                          tGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGcc                                                                                      >  2:3067953/1‑150 (MQ=255)
                                                          tGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGcc                                                                                      <  2:729155/150‑1 (MQ=255)
                                                            tCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCtt                                                                                    <  1:1004884/150‑1 (MQ=255)
                                                               tataACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTAc                                                                                 >  2:3627974/1‑150 (MQ=255)
                                                                ataACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACg                                                                                <  2:4028764/150‑1 (MQ=255)
                                                                ataACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACg                                                                                <  1:340166/150‑1 (MQ=255)
                                                                    ccATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCa                                                                            >  1:3560239/1‑150 (MQ=255)
                                                                     cATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCAt                                                                           >  1:4157142/1‑150 (MQ=255)
                                                                          aGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACa                                                                      <  2:4328759/150‑1 (MQ=255)
                                                                          aGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACa                                                                      >  2:4560706/1‑150 (MQ=255)
                                                                          aGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACa                                                                      >  1:111142/1‑150 (MQ=255)
                                                                           gACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAg                                                                     <  1:3748337/150‑1 (MQ=255)
                                                                            aCATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGc                                                                    >  2:154292/1‑150 (MQ=255)
                                                                            aCATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGc                                                                    >  1:255642/1‑150 (MQ=255)
                                                                             cATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCa                                                                   >  2:3691179/1‑150 (MQ=255)
                                                                              aTGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCAt                                                                  <  2:3514735/150‑1 (MQ=255)
                                                                                ggTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATag                                                                >  1:4839355/1‑150 (MQ=255)
                                                                                 gTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATaga                                                               >  1:2427857/1‑150 (MQ=255)
                                                                                   tAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAgaga                                                             <  1:3390762/150‑1 (MQ=255)
                                                                                   tAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAgaga                                                             <  1:4293724/150‑1 (MQ=255)
                                                                                      tgtTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATa                                                          >  2:2637709/1‑150 (MQ=255)
                                                                                      tgtTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTAGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATa                                                          >  2:902682/1‑150 (MQ=255)
                                                                                       gtTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATaa                                                         <  2:650202/150‑1 (MQ=255)
                                                                                          tCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAagg                                                      <  2:2065134/150‑1 (MQ=255)
                                                                                            ttGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAagggc                                                    <  1:2014280/150‑1 (MQ=255)
                                                                                             tGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAagggca                                                   >  2:2553556/1‑150 (MQ=255)
                                                                                               tcattcatCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAagggcagg                                                 >  2:2759682/1‑150 (MQ=255)
                                                                                                  ttcatCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAgggcagggca                                              <  2:1605825/150‑1 (MQ=255)
                                                                                                   tcatcaCAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCaa                                             >  2:2726708/1‑150 (MQ=255)
                                                                                                    catcaCAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAg                                            <  2:922080/150‑1 (MQ=255)
                                                                                                     atcaCAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGt                                           <  2:1919322/150‑1 (MQ=255)
                                                                                                      tcaCAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTa                                          <  1:3374119/150‑1 (MQ=255)
                                                                                                         caATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTacca                                       >  1:2094394/1‑150 (MQ=255)
                                                                                                           atatAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTaccacc                                     >  2:1374453/1‑150 (MQ=255)
                                                                                                              taGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAgg                                  <  2:3057702/150‑1 (MQ=255)
                                                                                                                   tggtgAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACt                             <  1:4436325/150‑1 (MQ=255)
                                                                                                                    ggtgAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTa                            <  1:2490449/150‑1 (MQ=255)
                                                                                                                     gtgAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTaa                           >  2:2134343/1‑150 (MQ=255)
                                                                                                                       gAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTaaca                         <  2:2843021/150‑1 (MQ=255)
                                                                                                                          cGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAAcc                      >  2:4502416/1‑150 (MQ=255)
                                                                                                                             gCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGt                   >  2:894906/1‑150 (MQ=255)
                                                                                                                                 gcgGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAAtt               <  2:3117920/150‑1 (MQ=255)
                                                                                                                                   gggTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCa             >  2:3839615/1‑150 (MQ=255)
                                                                                                                                   gggTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCa             >  2:3839719/1‑150 (MQ=255)
                                                                                                                                     gTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAac           <  1:1860236/150‑1 (MQ=255)
                                                                                                                                     gTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAac           >  2:549100/1‑150 (MQ=255)
                                                                                                                                      tGCAGGAAGAAGTGACC‑TTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAacat         <  2:3923928/150‑1 (MQ=255)
                                                                                                                                        cAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAacata        >  2:1801582/1‑150 (MQ=255)
                                                                                                                                        cAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAacata        <  2:3861147/150‑1 (MQ=255)
                                                                                                                                         aGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAacatac       <  1:4856420/150‑1 (MQ=255)
                                                                                                                                           gaagaaGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAacatacat     <  2:3720703/150‑1 (MQ=255)
                                                                                                                                            aagaagTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAAcatacata    >  1:2096430/1‑150 (MQ=255)
                                                                                                                                            aagaagTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAAcatacata    >  1:63498/1‑150 (MQ=255)
                                                                                                                                                   |                                                                                                                                                
CCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATATC  >  CP009273/1563129‑1563420

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATA  >  CP009273/1563127‑1563418
                                                                                                                                                     |                                                                                                                                              
TCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGACCGTGCACGCGGGTGCAGGAAGAAGTT                                                                                                                                                >  SRR19973081.3996264/1‑150 (MQ=60)
 cccatTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTA                                                                                                                                               <  SRR19973081.3186447/145‑1 (MQ=60)
 CCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTA                                                                                                                                               <  SRR19973081.4622780/150‑1 (MQ=60)
 CCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTA                                                                                                                                               <  SRR19973081.3186451/150‑1 (MQ=60)
  CCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGAC                                                                                                                                              >  SRR19973081.2357983/1‑150 (MQ=60)
  CCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGAC                                                                                                                                              >  SRR19973081.2423829/1‑150 (MQ=60)
  CCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGAC                                                                                                                                              >  SRR19973081.2424020/1‑150 (MQ=60)
   CCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACC                                                                                                                                             <  SRR19973081.4033085/150‑1 (MQ=60)
     GTTAAGTCAATACGACAGCAAGCACGAAACAGGGAGCGATGAATTATCGCTCCCTTGACTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTT                                                                                                                                           <  SRR19973081.2600280/150‑1 (MQ=60)
      TTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTT                                                                                                                                          >  SRR19973081.3531034/1‑150 (MQ=60)
       TAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTT                                                                                                                                         >  SRR19973081.2770316/1‑150 (MQ=60)
         AGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGG                                                                                                                                       <  SRR19973081.713008/150‑1 (MQ=60)
          GTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAAATATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGATTCTTGTCATTCATCACAATATAGTGTGGTGAAAGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGTT                                                                                                                                      >  SRR19973081.1004790/1‑150 (MQ=60)
           TCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTG                                                                                                                                     >  SRR19973081.2450169/1‑150 (MQ=60)
            CAATACGACAGCAAGCACGAAAAAGGGAGCGATGAAGTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCATGAAGAAGTTACCTTTTGGTGC                                                                                                                                    <  SRR19973081.610880/150‑1 (MQ=60)
                ACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTC                                                                                                                                <  SRR19973081.2681968/150‑1 (MQ=60)
                ACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTC                                                                                                                                <  SRR19973081.2682212/150‑1 (MQ=60)
                  GACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTG                                                                                                                              >  SRR19973081.3597468/1‑150 (MQ=60)
                  GACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTG                                                                                                                              <  SRR19973081.2134759/150‑1 (MQ=60)
                     AGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACT                                                                                                                           >  SRR19973081.73191/1‑150 (MQ=60)
                         AGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTG                                                                                                                       >  SRR19973081.3837221/1‑150 (MQ=60)
                          GCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGA                                                                                                                      <  SRR19973081.2821967/150‑1 (MQ=60)
                           CACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGAT                                                                                                                     >  SRR19973081.2383177/1‑150 (MQ=60)
                                AAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCT                                                                                                                >  SRR19973081.2860254/1‑150 (MQ=60)
                                 AAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTC                                                                                                               >  SRR19973081.1791356/1‑150 (MQ=60)
                                  AAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCC                                                                                                              <  SRR19973081.2025840/150‑1 (MQ=60)
                                     GGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGA                                                                                                           <  SRR19973081.4040979/150‑1 (MQ=60)
                                     GGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGA                                                                                                           <  SRR19973081.3421373/150‑1 (MQ=60)
                                         CGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGA                                                                                                       <  SRR19973081.3636534/150‑1 (MQ=60)
                                          GATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGAC                                                                                                      <  SRR19973081.4515301/150‑1 (MQ=60)
                                           ATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACG                                                                                                     >  SRR19973081.2459797/1‑150 (MQ=60)
                                           ATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACG                                                                                                     >  SRR19973081.768586/1‑150 (MQ=60)
                                           ATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACG                                                                                                     >  SRR19973081.1262547/1‑150 (MQ=60)
                                           ATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACG                                                                                                     >  SRR19973081.1262604/1‑150 (MQ=60)
                                              AATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCG                                                                                                  >  SRR19973081.397250/1‑150 (MQ=60)
                                              AATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCG                                                                                                  >  SRR19973081.131626/1‑150 (MQ=60)
                                              AATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCG                                                                                                  >  SRR19973081.397316/1‑150 (MQ=60)
                                               ATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGG                                                                                                 >  SRR19973081.1483244/1‑150 (MQ=60)
                                                TTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGT                                                                                                >  SRR19973081.3076511/1‑150 (MQ=60)
                                                TTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGT                                                                                                >  SRR19973081.3976795/1‑150 (MQ=60)
                                                   TCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATT                                                                                             <  SRR19973081.1064369/150‑1 (MQ=60)
                                                    CGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTT                                                                                            >  SRR19973081.1903833/1‑150 (MQ=60)
                                                    CGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTT                                                                                            >  SRR19973081.2693751/1‑150 (MQ=60)
                                                       TCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCT                                                                                         <  SRR19973081.3530077/150‑1 (MQ=60)
                                                            TGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCC                                                                                    >  SRR19973081.2704980/1‑150 (MQ=60)
                                                              TCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTT                                                                                  <  SRR19973081.1011699/150‑1 (MQ=60)
                                                                  ATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACG                                                                              <  SRR19973081.342560/150‑1 (MQ=60)
                                                                  ATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACG                                                                              <  SRR19973081.342670/150‑1 (MQ=60)
                                                                     ACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATC                                                                           >  SRR19973081.4320763/1‑150 (MQ=60)
                                                                     ACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATC                                                                           >  SRR19973081.3657022/1‑150 (MQ=60)
                                                                      CCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCA                                                                          >  SRR19973081.3581957/1‑150 (MQ=60)
                                                                       CATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCAT                                                                         >  SRR19973081.4182541/1‑150 (MQ=60)
                                                                         TTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGA                                                                       >  SRR19973081.2092772/1‑150 (MQ=60)
                                                                         TTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGA                                                                       >  SRR19973081.2115492/1‑150 (MQ=60)
                                                                         TTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGA                                                                       >  SRR19973081.2115735/1‑150 (MQ=60)
                                                                            AGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACA                                                                    >  SRR19973081.135509/1‑150 (MQ=60)
                                                                            AGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACA                                                                    >  SRR19973081.135513/1‑150 (MQ=60)
                                                                            AGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACA                                                                    >  SRR19973081.112267/1‑150 (MQ=60)
                                                                             GACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAG                                                                   <  SRR19973081.3771313/150‑1 (MQ=60)
                                                                             GACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAG                                                                   <  SRR19973081.3771298/150‑1 (MQ=60)
                                                                              ACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGC                                                                  >  SRR19973081.257715/1‑150 (MQ=60)
                                                                                  GGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAG                                                              >  SRR19973081.4868433/1‑150 (MQ=60)
                                                                                  GGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAG                                                              >  SRR19973081.4868508/1‑150 (MQ=60)
                                                                                   GTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGA                                                             >  SRR19973081.2442738/1‑150 (MQ=60)
                                                                                     TAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGA                                                           <  SRR19973081.4319886/150‑1 (MQ=60)
                                                                                     TAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGA                                                           <  SRR19973081.4317391/150‑1 (MQ=60)
                                                                                     TAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGA                                                           <  SRR19973081.3411610/150‑1 (MQ=60)
                                                                                            TCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGG                                                    >  SRR19973081.3233007/1‑150 (MQ=60)
                                                                                              TTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGC                                                  <  SRR19973081.2026819/150‑1 (MQ=60)
                                                                                                        TCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTA                                        <  SRR19973081.3394898/150‑1 (MQ=60)
                                                                                                        TCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTA                                        <  SRR19973081.3394866/150‑1 (MQ=60)
                                                                                                        TCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTA                                        >  SRR19973081.251298/1‑150 (MQ=60)
                                                                                                           CAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCA                                     >  SRR19973081.2107715/1‑150 (MQ=60)
                                                                                                               ATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAG                                 >  SRR19973081.2139057/1‑150 (MQ=60)
                                                                                                                GAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGG                                <  SRR19973081.2859006/150‑1 (MQ=60)
                                                                                                                     TGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACT                           <  SRR19973081.4463006/150‑1 (MQ=60)
                                                                                                                      GGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTA                          <  SRR19973081.2505952/150‑1 (MQ=60)
                                                                                                                           ACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAAC                     >  SRR19973081.3884882/1‑150 (MQ=60)
                                                                                                                            CGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACC                    >  SRR19973081.3702711/1‑150 (MQ=60)
                                                                                                                            CGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACC                    >  SRR19973081.1225567/1‑150 (MQ=60)
                                                                                                                             GTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCA                   >  SRR19973081.1822379/1‑150 (MQ=60)
                                                                                                                                CACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTA                <  SRR19973081.258308/150‑1 (MQ=60)
                                                                                                                                       GTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAAC         <  SRR19973081.1872262/150‑1 (MQ=60)
                                                                                                                                           AGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATAC     <  SRR19973081.4885561/150‑1 (MQ=60)
                                                                                                                                           AGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATAC     <  SRR19973081.3448608/150‑1 (MQ=60)
                                                                                                                                           AGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATAC     <  SRR19973081.2654134/150‑1 (MQ=60)
                                                                                                                                            GGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACA    >  SRR19973081.1654582/1‑150 (MQ=60)
                                                                                                                                              AAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATA  >  SRR19973081.2109403/1‑150 (MQ=60)
                                                                                                                                              AAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATA  <  SRR19973081.4860665/150‑1 (MQ=60)
                                                                                                                                              AAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATA  >  SRR19973081.2109776/1‑150 (MQ=60)
                                                                                                                                              AAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATA  >  SRR19973081.64238/1‑150 (MQ=60)
                                                                                                                                                     |                                                                                                                                              
TCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATA  >  CP009273/1563127‑1563418

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑