Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F204 I0 R1 1155 255.8 8356456 95.7% 7997128 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,563,276 G→T 100% H491N (CAC→AAC)  gadC ← glutamate:gamma‑aminobutyric acid antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,563,2760GT97.3% 747.0 / 10.0 223H491N (CAC→AAC) gadCglutamate:gamma‑aminobutyric acid antiporter
Reads supporting (aligned to +/- strand):  ref base G (2/4);  new base T (110/107);  total (112/111)
Fisher's exact test for biased strand distribution p-value = 4.46e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.23e-01
Rejected as polymorphism: Frequency below/above cutoff threshold.

Only 100 of 223 total aligned reads displayed.

CCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATATCGG  >  CP009273/1563129‑1563422
                                                                                                                                                   |                                                                                                                                                  
cccGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTAc                                                                                                                                                  >  1:4113723/1‑150 (MQ=255)
 ccGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTAcc                                                                                                                                                 <  2:3089888/150‑1 (MQ=255)
 ccGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTAcc                                                                                                                                                 >  1:1225250/1‑150 (MQ=255)
    ttAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCttt                                                                                                                                              <  1:2585435/150‑1 (MQ=255)
      aaGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTg                                                                                                                                            <  2:3837226/150‑1 (MQ=255)
      aaGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTg                                                                                                                                            <  1:2268962/150‑1 (MQ=255)
        gTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGt                                                                                                                                          <  1:2086848/150‑1 (MQ=255)
         tCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTg                                                                                                                                         >  2:1188287/1‑150 (MQ=255)
         tCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTg                                                                                                                                         >  2:3633909/1‑150 (MQ=255)
           aaTACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCg                                                                                                                                       >  2:1614062/1‑150 (MQ=255)
               cGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCCCAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCt                                                                                                                                   <  2:4102656/150‑1 (MQ=255)
               cGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCt                                                                                                                                   <  1:2832709/150‑1 (MQ=255)
               cGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCt                                                                                                                                   <  2:2597980/150‑1 (MQ=255)
               cGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCt                                                                                                                                   <  2:2597974/150‑1 (MQ=255)
                gACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTg                                                                                                                                  >  2:420941/1‑150 (MQ=255)
                gACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTg                                                                                                                                  >  2:3338219/1‑150 (MQ=255)
                 aCAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGa                                                                                                                                 >  1:902271/1‑150 (MQ=255)
                 aCAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGa                                                                                                                                 <  1:3808783/150‑1 (MQ=255)
                 aCAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGa                                                                                                                                 <  1:2173113/150‑1 (MQ=255)
                   agcaagcaCGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACt                                                                                                                               >  1:3754746/1‑150 (MQ=255)
                      nagcaCGANAAAGGGANCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTAATCACACTATAGTGTGGTGAACGTGCACGNGGGTGCAGGAAGACGTTACCTTTTGGTGCGTTCTGACTGtt                                                                                                                            >  2:3313702/2‑150 (MQ=255)
                      aagcaCGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGtt                                                                                                                            >  1:1561067/1‑150 (MQ=255)
                        gcaCGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGa                                                                                                                          <  2:3527844/150‑1 (MQ=255)
                           cGAAAAAGTGAGCTATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTTGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTg                                                                                                                       <  1:3404923/150‑1 (MQ=255)
                              aaaaGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCt                                                                                                                    >  2:2876424/1‑150 (MQ=255)
                               aaaGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTc                                                                                                                   >  2:1999356/1‑150 (MQ=255)
                                 aGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCa                                                                                                                 >  1:2238726/1‑150 (MQ=255)
                                 aGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCa                                                                                                                 <  2:2167855/150‑1 (MQ=255)
                                  gggAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCag                                                                                                                <  2:1519026/150‑1 (MQ=255)
                                  gggAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTGTTGGTGCGTTCTGACTGTTGATTGGCTCCag                                                                                                                >  2:2063397/1‑150 (MQ=255)
                                      gCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTg                                                                                                            <  1:1969032/150‑1 (MQ=255)
                                       cGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGa                                                                                                           <  1:180686/150‑1 (MQ=255)
                                       cGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGa                                                                                                           <  2:3435073/150‑1 (MQ=255)
                                         aTGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACg                                                                                                         <  2:2204526/150‑1 (MQ=255)
                                          tGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGc                                                                                                        >  2:440917/1‑150 (MQ=255)
                                          tGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGc                                                                                                        >  2:714613/1‑150 (MQ=255)
                                               tATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTa                                                                                                   >  2:1344136/1‑150 (MQ=255)
                                               tATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTa                                                                                                   <  2:1171978/150‑1 (MQ=255)
                                                  cGCTCCCTTGTCTTATAACCATTCAGACAGGGTTAGTGTTTCTGGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTAttt                                                                                                <  1:1443285/150‑1 (MQ=255)
                                                   gCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTAtttg                                                                                               >  1:3290044/1‑150 (MQ=255)
                                                     tCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGTTGCGTTCTGACTGTTGATTGGCTCCAGATTGACGCCGTTAtttgct                                                                                             >  1:1135314/1‑150 (MQ=255)
                                                     tCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTAtttgct                                                                                             >  1:4010883/1‑150 (MQ=255)
                                                       ccTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTAtttgcttt                                                                                           <  2:2796572/150‑1 (MQ=255)
                                                        cTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTAtttgctttg                                                                                          >  1:1283247/1‑150 (MQ=255)
                                                        cTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTAtttgctttg                                                                                          >  1:685219/1‑150 (MQ=255)
                                                             cTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCttt                                                                                     <  1:2693571/150‑1 (MQ=255)
                                                              ttATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTa                                                                                    >  2:2251581/1‑150 (MQ=255)
                                                               tataACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTAc                                                                                   >  1:3432422/1‑150 (MQ=255)
                                                                ataCCCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACg                                                                                  >  2:2665113/1‑150 (MQ=255)
                                                                 taACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGa                                                                                 >  2:4011572/1‑150 (MQ=255)
                                                                  aaCCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGAt                                                                                <  1:1381875/150‑1 (MQ=255)
                                                                     cATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCAt                                                                             <  2:1395178/150‑1 (MQ=255)
                                                                       ttCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGa                                                                           >  1:1041486/1‑150 (MQ=255)
                                                                          aGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACa                                                                        >  1:2919622/1‑150 (MQ=255)
                                                                          aGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACa                                                                        <  1:2327645/150‑1 (MQ=255)
                                                                                ggTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATag                                                                  >  1:171903/1‑150 (MQ=255)
                                                                                   tAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAgaga                                                               >  1:3842354/1‑150 (MQ=255)
                                                                                    aGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGaa                                                              <  1:1353313/150‑1 (MQ=255)
                                                                                     gtgtTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAAt                                                             <  1:2251581/150‑1 (MQ=255)
                                                                                      tgtTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATa                                                            >  2:2964673/1‑150 (MQ=255)
                                                                                       gtTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCATTACGATCATGAACAGCATAGAGAATaa                                                           <  1:2082142/150‑1 (MQ=255)
                                                                                         ttCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAag                                                         >  1:1675682/1‑150 (MQ=255)
                                                                                              gTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAagggcag                                                    <  2:588074/150‑1 (MQ=255)
                                                                                              gTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAagggcag                                                    <  1:3517481/150‑1 (MQ=255)
                                                                                               tcattcatCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAagggcagg                                                   >  2:1069677/1‑150 (MQ=255)
                                                                                                     atcaCAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGt                                             >  2:2507408/1‑150 (MQ=255)
                                                                                                          aaTATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTaccac                                        >  2:792029/1‑150 (MQ=255)
                                                                                                           atatAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTaccacc                                       <  1:1106474/150‑1 (MQ=255)
                                                                                                           atatAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTaccacc                                       <  2:1897428/150‑1 (MQ=255)
                                                                                                            tataGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTAccacca                                      >  1:3176045/1‑150 (MQ=255)
                                                                                                              taGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAgg                                    >  1:2146966/1‑150 (MQ=255)
                                                                                                                    ggtgAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTa                              >  2:861015/1‑150 (MQ=255)
                                                                                                                    ggtgAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTa                              <  1:2454162/150‑1 (MQ=255)
                                                                                                                    ggtgAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTa                              >  2:1481779/1‑150 (MQ=255)
                                                                                                                        aaCGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTaacaa                          <  2:3443900/150‑1 (MQ=255)
                                                                                                                        aaCGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTaacaa                          <  2:1350680/150‑1 (MQ=255)
                                                                                                                         aCGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTaacaac                         <  2:2256625/150‑1 (MQ=255)
                                                                                                                           gTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCa                       >  1:3592293/1‑150 (MQ=255)
                                                                                                                           gTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCa                       >  2:4035152/1‑150 (MQ=255)
                                                                                                                            tGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAg                      <  2:2588181/150‑1 (MQ=255)
                                                                                                                            tGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAg                      <  1:1887178/150‑1 (MQ=255)
                                                                                                                             gCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGt                     <  2:3941697/150‑1 (MQ=255)
                                                                                                                               aCGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTaa                   >  1:2987938/1‑150 (MQ=255)
                                                                                                                                cgcgGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAAt                  <  1:496183/150‑1 (MQ=255)
                                                                                                                                cgcgGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAAt                  <  2:3881846/150‑1 (MQ=255)
                                                                                                                                  cgGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTc                >  1:1309258/1‑150 (MQ=255)
                                                                                                                                   gggTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCa               <  1:2865888/150‑1 (MQ=255)
                                                                                                                                    ggTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCaa              >  2:1587336/1‑150 (MQ=255)
                                                                                                                                    ggTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCaa              <  2:3409096/150‑1 (MQ=255)
                                                                                                                                    ggTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCaa              <  2:2058949/150‑1 (MQ=255)
                                                                                                                                      tGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAaca            <  2:1803992/150‑1 (MQ=255)
                                                                                                                                      tGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAaca            <  1:3197038/150‑1 (MQ=255)
                                                                                                                                      tGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAaca            <  2:2039723/150‑1 (MQ=255)
                                                                                                                                        cAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAacata          <  1:299479/150‑1 (MQ=255)
                                                                                                                                        cAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAacata          <  1:295350/150‑1 (MQ=255)
                                                                                                                                            aagaagTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAAcatacata      <  2:866607/150‑1 (MQ=255)
                                                                                                                                            aagaagTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAAcatacata      >  1:2844077/1‑150 (MQ=255)
                                                                                                                                             agaagTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACatat     <  1:2670502/150‑1 (MQ=255)
                                                                                                                                               aagTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATATCg   <  2:3183463/150‑1 (MQ=255)
                                                                                                                                                agTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATATCgg  <  1:3735975/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATATCGG  >  CP009273/1563129‑1563422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATATCGGT  >  CP009273/1563128‑1563423
                                                                                                                                                    |                                                                                                                                                   
CCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTA                                                                                                                                                    <  SRR19973074.1657318/150‑1 (MQ=60)
CCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTA                                                                                                                                                    <  SRR19973074.442145/150‑1 (MQ=60)
CCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTA                                                                                                                                                    <  SRR19973074.3824359/150‑1 (MQ=60)
 CCCGTTAAGTCAATACGACAGCAAGCACGAAACAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGGGAACGTGCACGCGGGTGCAGGAAGAAGTTAC                                                                                                                                                   <  SRR19973074.1431585/150‑1 (MQ=60)
 CCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTAC                                                                                                                                                   >  SRR19973074.1305657/1‑150 (MQ=60)
 CCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTAC                                                                                                                                                   >  SRR19973074.4121316/1‑150 (MQ=60)
 CCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTAC                                                                                                                                                   >  SRR19973074.4121317/1‑150 (MQ=60)
  CCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACC                                                                                                                                                  >  SRR19973074.1227598/1‑150 (MQ=60)
  CCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACC                                                                                                                                                  <  SRR19973074.1060695/150‑1 (MQ=60)
     TTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTT                                                                                                                                               <  SRR19973074.2590264/150‑1 (MQ=60)
       AAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTG                                                                                                                                             <  SRR19973074.2273224/150‑1 (MQ=60)
         GTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGT                                                                                                                                           <  SRR19973074.2090914/150‑1 (MQ=60)
         GTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGT                                                                                                                                           <  SRR19973074.2090756/150‑1 (MQ=60)
               ACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTC                                                                                                                                     <  SRR19973074.2733382/150‑1 (MQ=60)
                CGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCT                                                                                                                                    <  SRR19973074.2837950/150‑1 (MQ=60)
                CGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCT                                                                                                                                    >  SRR19973074.2257301/1‑150 (MQ=60)
                  ACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGA                                                                                                                                  <  SRR19973074.3815746/150‑1 (MQ=60)
                  ACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGA                                                                                                                                  >  SRR19973074.903991/1‑150 (MQ=60)
                  ACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGA                                                                                                                                  <  SRR19973074.2177197/150‑1 (MQ=60)
                    AGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACT                                                                                                                                >  SRR19973074.3761637/1‑150 (MQ=60)
                    AGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACT                                                                                                                                >  SRR19973074.3761646/1‑150 (MQ=60)
                       AAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTT                                                                                                                             >  SRR19973074.1564028/1‑150 (MQ=60)
                       AAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTT                                                                                                                             >  SRR19973074.1564037/1‑150 (MQ=60)
                       AAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTT                                                                                                                             >  SRR19973074.1475179/1‑150 (MQ=60)
                         GCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGA                                                                                                                           >  SRR19973074.1940216/1‑150 (MQ=60)
                          CACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGAT                                                                                                                          <  SRR19973074.366966/150‑1 (MQ=60)
                            CGAAAAAGTGAGCTATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTTGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTG                                                                                                                        <  SRR19973074.3411220/150‑1 (MQ=60)
                            CGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTG                                                                                                                        <  SRR19973074.3135645/150‑1 (MQ=60)
                            CGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTG                                                                                                                        <  SRR19973074.3411224/150‑1 (MQ=60)
                            CGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTG                                                                                                                        >  SRR19973074.3594987/1‑150 (MQ=60)
                               AAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCT                                                                                                                     <  SRR19973074.672905/150‑1 (MQ=60)
                               AAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCT                                                                                                                     >  SRR19973074.3514414/1‑150 (MQ=60)
                                AAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTC                                                                                                                    >  SRR19973074.2166550/1‑150 (MQ=60)
                                AAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTC                                                                                                                    >  SRR19973074.2166514/1‑150 (MQ=60)
                                  AGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCA                                                                                                                  >  SRR19973074.2242936/1‑150 (MQ=60)
                                     GAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAG                                                                                                               >  SRR19973074.3844926/1‑150 (MQ=60)
                                     GAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAG                                                                                                               <  SRR19973074.2204556/150‑1 (MQ=60)
                                       GCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTG                                                                                                             <  SRR19973074.1972786/150‑1 (MQ=60)
                                        CGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGA                                                                                                            <  SRR19973074.181074/150‑1 (MQ=60)
                                        CGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGA                                                                                                            <  SRR19973074.181088/150‑1 (MQ=60)
                                                 ATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTAT                                                                                                   <  SRR19973074.2798706/150‑1 (MQ=60)
                                                  TCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATT                                                                                                  >  SRR19973074.1234836/1‑150 (MQ=60)
                                                   CGCTCCCTTGTCTTATAACCATTCAGACAGGGTTAGTGTTTCTGGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTT                                                                                                 <  SRR19973074.1445981/150‑1 (MQ=60)
                                                    GCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTG                                                                                                >  SRR19973074.3296151/1‑150 (MQ=60)
                                                      TCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGTTGCGTTCTGACTGTTGATTGGCTCCAGATTGACGCCGTTATTTGCT                                                                                              >  SRR19973074.1137466/1‑150 (MQ=60)
                                                      TCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCT                                                                                              >  SRR19973074.1137436/1‑150 (MQ=60)
                                                      TCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCT                                                                                              >  SRR19973074.1137554/1‑150 (MQ=60)
                                                      TCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCT                                                                                              >  SRR19973074.4018301/1‑150 (MQ=60)
                                                        CCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTT                                                                                            <  SRR19973074.13358/150‑1 (MQ=60)
                                                         CTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTG                                                                                           >  SRR19973074.686476/1‑150 (MQ=60)
                                                         CTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTG                                                                                           >  SRR19973074.1285689/1‑150 (MQ=60)
                                                           TGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCC                                                                                         <  SRR19973074.1910767/150‑1 (MQ=60)
                                                              CTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTT                                                                                      <  SRR19973074.2698247/150‑1 (MQ=60)
                                                              CTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTT                                                                                      <  SRR19973074.2698575/150‑1 (MQ=60)
                                                                TATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTAC                                                                                    >  SRR19973074.3438746/1‑150 (MQ=60)
                                                                TATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTAC                                                                                    >  SRR19973074.2920632/1‑150 (MQ=60)
                                                                TATAACCATTCAGACATGGTTAGTGTATCTTGTCATTCATCACAATATAGTGTGGTGAACGAGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTAGCCTTTAC                                                                                    >  SRR19973074.2948348/1‑150 (MQ=60)
                                                                   AACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGAT                                                                                 <  SRR19973074.1384423/150‑1 (MQ=60)
                                                                   AACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGAT                                                                                 <  SRR19973074.1384420/150‑1 (MQ=60)
                                                                       ATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATG                                                                             <  SRR19973074.849647/150‑1 (MQ=60)
                                                                        TTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGA                                                                            >  SRR19973074.3277646/1‑150 (MQ=60)
                                                                        TTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGA                                                                            >  SRR19973074.1043400/1‑150 (MQ=60)
                                                                           AGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACA                                                                         <  SRR19973074.2314891/150‑1 (MQ=60)
                                                                           AGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACA                                                                         <  SRR19973074.2331989/150‑1 (MQ=60)
                                                                           AGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACA                                                                         <  SRR19973074.2369248/150‑1 (MQ=60)
                                                                           AGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGATCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACA                                                                         >  SRR19973074.2925051/1‑150 (MQ=60)
                                                                             ACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGC                                                                       >  SRR19973074.2838580/1‑150 (MQ=60)
                                                                                 GGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAG                                                                   >  SRR19973074.172278/1‑150 (MQ=60)
                                                                                 GGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAG                                                                   >  SRR19973074.172286/1‑150 (MQ=60)
                                                                                 GGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAG                                                                   >  SRR19973074.2254532/1‑150 (MQ=60)
                                                                                    TAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGA                                                                >  SRR19973074.3849354/1‑150 (MQ=60)
                                                                                     AGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAA                                                               <  SRR19973074.1355831/150‑1 (MQ=60)
                                                                                      GTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAAT                                                              <  SRR19973074.2255813/150‑1 (MQ=60)
                                                                                        GTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAA                                                            <  SRR19973074.2086072/150‑1 (MQ=60)
                                                                                        GTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCATTACGATCATGAACAGCATAGAGAATAA                                                            <  SRR19973074.2086039/150‑1 (MQ=60)
                                                                                          TTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAG                                                          >  SRR19973074.1678828/1‑150 (MQ=60)
                                                                                          TTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAG                                                          >  SRR19973074.1678830/1‑150 (MQ=60)
                                                                                               GTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAG                                                     <  SRR19973074.3523943/150‑1 (MQ=60)
                                                                                                TCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGG                                                    >  SRR19973074.379610/1‑150 (MQ=60)
                                                                                                    TCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAA                                                <  SRR19973074.248124/150‑1 (MQ=60)
                                                                                                      ATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGT                                              >  SRR19973074.3841330/1‑150 (MQ=60)
                                                                                                           AATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCAC                                         >  SRR19973074.603844/1‑150 (MQ=60)
                                                                                                            ATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACC                                        <  SRR19973074.1108552/150‑1 (MQ=60)
                                                                                                             TATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCA                                       >  SRR19973074.3181891/1‑150 (MQ=60)
                                                                                                               TAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGG                                     >  SRR19973074.2151004/1‑150 (MQ=60)
                                                                                                                   GTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAAC                                 <  SRR19973074.3389808/150‑1 (MQ=60)
                                                                                                                   GTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAAC                                 >  SRR19973074.2075157/1‑150 (MQ=60)
                                                                                                                     GGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTA                               <  SRR19973074.2458699/150‑1 (MQ=60)
                                                                                                                         ATCGTGCACGCGGGTGCAGTAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGCTTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAA                           <  SRR19973074.1307250/150‑1 (MQ=60)
                                                                                                                            GTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCA                        >  SRR19973074.3598926/1‑150 (MQ=60)
                                                                                                                             TGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAG                       <  SRR19973074.1890790/150‑1 (MQ=60)
                                                                                                                               CACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTA                     <  SRR19973074.2946357/150‑1 (MQ=60)
                                                                                                                                ACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAA                    >  SRR19973074.2993630/1‑150 (MQ=60)
                                                                                                                                ACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAA                    >  SRR19973074.2993491/1‑150 (MQ=60)
                                                                                                                                ACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAA                    >  SRR19973074.2993488/1‑150 (MQ=60)
                                                                                                                                 CGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAAT                   <  SRR19973074.497180/150‑1 (MQ=60)
                                                                                                                                   CGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTC                 >  SRR19973074.1311724/1‑150 (MQ=60)
                                                                                                                                    GGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCA                <  SRR19973074.2871208/150‑1 (MQ=60)
                                                                                                                                       TGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACA             <  SRR19973074.3202918/150‑1 (MQ=60)
                                                                                                                                       TGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACA             <  SRR19973074.3202932/150‑1 (MQ=60)
                                                                                                                                       TGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACA             <  SRR19973074.3202882/150‑1 (MQ=60)
                                                                                                                                        GCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACAT            >  SRR19973074.230985/1‑150 (MQ=60)
                                                                                                                                         CAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATA           <  SRR19973074.295898/150‑1 (MQ=60)
                                                                                                                                         CAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATA           <  SRR19973074.300036/150‑1 (MQ=60)
                                                                                                                                          AGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATAC          <  SRR19973074.4112535/150‑1 (MQ=60)
                                                                                                                                             AAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATC       <  SRR19973074.3598332/150‑1 (MQ=60)
                                                                                                                                             AAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATA       >  SRR19973074.3972536/1‑150 (MQ=60)
                                                                                                                                             AAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATA       >  SRR19973074.2849381/1‑150 (MQ=60)
                                                                                                                                             AAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATA       >  SRR19973074.2849349/1‑150 (MQ=60)
                                                                                                                                              AGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATAT      <  SRR19973074.2675476/150‑1 (MQ=60)
                                                                                                                                              AGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATAT      <  SRR19973074.2675302/150‑1 (MQ=60)
                                                                                                                                                AAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATATCG    <  SRR19973074.1528507/150‑1 (MQ=60)
                                                                                                                                                 AGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATATCGG   <  SRR19973074.3742855/150‑1 (MQ=60)
                                                                                                                                                  GTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATATCGGT  <  SRR19973074.2782071/150‑1 (MQ=60)
                                                                                                                                                    |                                                                                                                                                   
CCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATATCGGT  >  CP009273/1563128‑1563423

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑