Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F78 I0 R1 626 241.3 10389380 77.7% 8072548 149.9

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,563,276 G→T 94.5% H491N (CAC→AAC)  gadC ← glutamate:gamma‑aminobutyric acid antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,563,2760GT94.5% 594.7 / 22.1 182H491N (CAC→AAC) gadCglutamate:gamma‑aminobutyric acid antiporter
Reads supporting (aligned to +/- strand):  ref base G (6/4);  new base T (92/80);  total (98/84)
Fisher's exact test for biased strand distribution p-value = 7.55e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.31e-01

Only 100 of 186 total aligned reads displayed.

TCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATATCGG  >  CP009273/1563127‑1563422
                                                                                                                                                     |                                                                                                                                                  
tCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGCAGAAGTg                                                                                                                                                    <  2:316556/150‑1 (MQ=255)
tCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTg                                                                                                                                                    <  2:316577/150‑1 (MQ=255)
    cGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCt                                                                                                                                                <  1:3921751/150‑1 (MQ=255)
     gTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCCCGCGGGTGCAGGAAGAAGTTACCtt                                                                                                                                               >  1:2166160/1‑150 (MQ=255)
     gTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCtt                                                                                                                                               >  1:4046175/1‑150 (MQ=255)
     gTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCtt                                                                                                                                               >  1:1844340/1‑150 (MQ=255)
       tAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCtttt                                                                                                                                             >  2:1689187/1‑150 (MQ=255)
         aGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTgg                                                                                                                                           >  2:4474134/1‑150 (MQ=255)
          gTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTGGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGACGTTACCTTTTGGt                                                                                                                                          >  2:2695098/1‑150 (MQ=255)
           tCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTg                                                                                                                                         >  2:2095794/1‑150 (MQ=255)
            cAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGc                                                                                                                                        <  1:4544091/150‑1 (MQ=255)
              aTACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGt                                                                                                                                      <  2:4102983/150‑1 (MQ=255)
                  gACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTg                                                                                                                                  >  2:1710320/1‑150 (MQ=255)
                   aCAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGa                                                                                                                                 >  1:772611/1‑150 (MQ=255)
                   aCAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGa                                                                                                                                 >  1:3376551/1‑150 (MQ=255)
                    cAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGAc                                                                                                                                >  2:3221574/1‑150 (MQ=255)
                          gcaCGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGa                                                                                                                          >  2:1006912/1‑150 (MQ=255)
                            aCGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGAtt                                                                                                                        <  2:3589404/150‑1 (MQ=255)
                            aCGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGAtt                                                                                                                        <  2:1307465/150‑1 (MQ=255)
                            aCGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGAtt                                                                                                                        <  2:3590122/150‑1 (MQ=255)
                              gAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTgg                                                                                                                      >  1:2125443/1‑150 (MQ=255)
                                  aaGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTcc                                                                                                                  >  2:4787165/1‑150 (MQ=255)
                                  aaGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTcc                                                                                                                  >  2:536630/1‑150 (MQ=255)
                                   aGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCa                                                                                                                 <  2:2677012/150‑1 (MQ=255)
                                    gggAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCag                                                                                                                >  1:4314866/1‑150 (MQ=255)
                                     tgAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGAGTGGCTCCaga                                                                                                               >  2:2162813/2‑150 (MQ=255)
                                       aGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGt                                                                                                             >  1:907636/1‑150 (MQ=255)
                                         cGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGa                                                                                                           >  2:4391564/1‑150 (MQ=255)
                                          gATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGAc                                                                                                          <  1:771410/150‑1 (MQ=255)
                                             gAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGcc                                                                                                       <  2:369612/150‑1 (MQ=255)
                                               aTTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCgg                                                                                                     >  2:4751350/1‑150 (MQ=255)
                                               aTTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCgg                                                                                                     >  1:1009209/1‑150 (MQ=255)
                                               aTTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCgg                                                                                                     >  1:2091951/1‑150 (MQ=255)
                                                   tCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTAtt                                                                                                 <  2:5065239/150‑1 (MQ=255)
                                                     gCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTAtttg                                                                                               >  1:2299312/1‑150 (MQ=255)
                                                         ccTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTAtttgcttt                                                                                           <  1:1911491/150‑1 (MQ=255)
                                                          cTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTAtttgctttg                                                                                          <  1:1181274/150‑1 (MQ=255)
                                                             gTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCt                                                                                       <  2:1756050/150‑1 (MQ=255)
                                                             gTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCt                                                                                       <  2:1148353/150‑1 (MQ=255)
                                                              tCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCtt                                                                                      <  1:2031131/150‑1 (MQ=255)
                                                              tCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCtt                                                                                      >  1:4642931/1‑150 (MQ=255)
                                                                 tataACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTAc                                                                                   >  1:3299013/1‑150 (MQ=255)
                                                                    aaCCATTCAGACCTGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGAt                                                                                >  2:305488/1‑150 (MQ=255)
                                                                         ttCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGa                                                                           >  2:910462/1‑150 (MQ=255)
                                                                           cAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAAc                                                                         >  1:3155715/1‑150 (MQ=255)
                                                                           cAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAAc                                                                         >  1:2365641/1‑150 (MQ=255)
                                                                            aGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACa                                                                        >  2:458352/1‑150 (MQ=255)
                                                                            aGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACa                                                                        <  1:588560/150‑1 (MQ=255)
                                                                             gACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAg                                                                       >  1:1551576/1‑150 (MQ=255)
                                                                               cATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCa                                                                     >  2:4959336/1‑150 (MQ=255)
                                                                                  ggTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATag                                                                  >  2:4395392/1‑150 (MQ=255)
                                                                                  ggTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATag                                                                  >  2:2597789/1‑150 (MQ=255)
                                                                                  ggTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATag                                                                  <  2:2996100/150‑1 (MQ=255)
                                                                                     tAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAgaga                                                               >  2:2590510/1‑150 (MQ=255)
                                                                                      aGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGaa                                                              <  2:1268772/150‑1 (MQ=255)
                                                                                       gtgtTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAAt                                                             <  2:3708267/150‑1 (MQ=255)
                                                                                       gtgtTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAAt                                                             <  1:240270/150‑1 (MQ=255)
                                                                                       gtgtTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAAt                                                             <  1:1759497/150‑1 (MQ=255)
                                                                                        tgtTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATa                                                            >  2:1243802/1‑150 (MQ=255)
                                                                                           ttCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAag                                                         <  1:3584843/150‑1 (MQ=255)
                                                                                           ttCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAag                                                         <  1:3584819/150‑1 (MQ=255)
                                                                                            tCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAagg                                                        <  2:1614402/150‑1 (MQ=255)
                                                                                            tCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAagg                                                        >  2:2888067/1‑150 (MQ=255)
                                                                                             cTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAaggg                                                       <  1:4987107/150‑1 (MQ=255)
                                                                                             cTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAaggg                                                       <  1:4987296/150‑1 (MQ=255)
                                                                                              ttGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAagggc                                                      >  1:2514115/1‑150 (MQ=255)
                                                                                               tGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAagggca                                                     >  2:2356039/1‑150 (MQ=255)
                                                                                                gTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAagggcag                                                    >  1:4911261/1‑150 (MQ=255)
                                                                                                gTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAagggcag                                                    >  1:4566338/1‑150 (MQ=255)
                                                                                                gTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAagggcag                                                    >  1:4566014/1‑150 (MQ=255)
                                                                                                  cattcatCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAagggcaggg                                                  <  1:2891144/150‑1 (MQ=255)
                                                                                                    ttcatCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAgggcagggca                                                >  2:5054285/1‑150 (MQ=255)
                                                                                                    ttcatCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAgggcagggca                                                <  2:717215/150‑1 (MQ=255)
                                                                                                       atcaCAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGt                                             >  2:2493238/1‑150 (MQ=255)
                                                                                                            aaTATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTaccac                                        >  1:2002459/1‑150 (MQ=255)
                                                                                                              tataGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTAccacca                                      <  2:2874936/150‑1 (MQ=255)
                                                                                                               ataGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGACTAAAGGGCAGGGCAAGTACCACCAg                                     >  2:3609419/1‑150 (MQ=255)
                                                                                                                  gtgtGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGaa                                  <  1:64083/150‑1 (MQ=255)
                                                                                                                  gtgtGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGaa                                  <  1:2681371/150‑1 (MQ=255)
                                                                                                                    gtggtgAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAAc                                >  1:4897617/1‑150 (MQ=255)
                                                                                                                    gtggtgAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAAc                                >  1:4940906/1‑150 (MQ=255)
                                                                                                                       gtgAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTaa                             <  1:3125699/150‑1 (MQ=255)
                                                                                                                        tgAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACTCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTaac                            <  2:4626349/150‑1 (MQ=255)
                                                                                                                        tgAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTaac                            >  1:1828707/1‑150 (MQ=255)
                                                                                                                        tgAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTGGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTaac                            <  2:3886246/150‑1 (MQ=255)
                                                                                                                         gAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTaaca                           <  2:1982138/150‑1 (MQ=255)
                                                                                                                          aaCGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTaacaa                          <  2:2085989/150‑1 (MQ=255)
                                                                                                                              tGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAg                      <  2:5021374/150‑1 (MQ=255)
                                                                                                                                cACGCGGGTGCAGGAAGAAGTTACCTTTTGTTGCGTTCTGACTGTTGATTGGCTCCAGTGTGACGCCGGTTTTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTa                    <  1:1309487/150‑1 (MQ=255)
                                                                                                                                cACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTa                    <  2:2299312/150‑1 (MQ=255)
                                                                                                                                      ggTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCaa              <  1:5069450/150‑1 (MQ=255)
                                                                                                                                           aggtattagTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAacatac         <  1:3221574/143‑1 (MQ=255)
                                                                                                                                           aGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAacatac         >  1:2156231/1‑150 (MQ=255)
                                                                                                                                           aGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAacatac         >  1:3534339/1‑150 (MQ=255)
                                                                                                                                           aGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAacatac         <  1:1006912/150‑1 (MQ=255)
                                                                                                                                           aGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAacatac         <  1:4101967/150‑1 (MQ=255)
                                                                                                                                            ggAAGAAGTTTCCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAacataca        <  1:469662/150‑1 (MQ=255)
                                                                                                                                               agaagTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACatat     >  1:344600/1‑150 (MQ=255)
                                                                                                                                                 aagTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATATCg   >  2:516445/1‑150 (MQ=255)
                                                                                                                                                  agTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATATCgg  >  2:1301737/1‑150 (MQ=255)
                                                                                                                                                     |                                                                                                                                                  
TCCCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATATCGG  >  CP009273/1563127‑1563422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATATCGGTA  >  CP009273/1563131‑1563424
                                                                                                                                                 |                                                                                                                                                    
CGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCT                                                                                                                                                  <  SRR19973083.3939207/150‑1 (MQ=60)
 GTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCCCGCGGGTGCAGGAAGAAGTTACCTT                                                                                                                                                 >  SRR19973083.2177018/1‑150 (MQ=60)
 GTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTT                                                                                                                                                 >  SRR19973083.1853640/1‑150 (MQ=60)
 GTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTT                                                                                                                                                 >  SRR19973083.4063794/1‑150 (MQ=60)
 GTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTT                                                                                                                                                 >  SRR19973083.4064155/1‑150 (MQ=60)
 GTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAAGATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTT                                                                                                                                                 >  SRR19973083.4065362/1‑150 (MQ=60)
   TAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTT                                                                                                                                               <  SRR19973083.984265/150‑1 (MQ=60)
     AGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGG                                                                                                                                             >  SRR19973083.1831278/1‑150 (MQ=60)
        CAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGC                                                                                                                                          <  SRR19973083.4563791/150‑1 (MQ=60)
             CGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCT                                                                                                                                     <  SRR19973083.789166/150‑1 (MQ=60)
               ACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACACTATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGA                                                                                                                                   >  SRR19973083.3392126/1‑150 (MQ=60)
               ACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGA                                                                                                                                   >  SRR19973083.776381/1‑150 (MQ=60)
               ACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGA                                                                                                                                   >  SRR19973083.776375/1‑150 (MQ=60)
               ACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGA                                                                                                                                   >  SRR19973083.3392221/1‑150 (MQ=60)
                     AGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTG                                                                                                                             <  SRR19973083.3553562/150‑1 (MQ=60)
                     AGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTG                                                                                                                             <  SRR19973083.2815585/150‑1 (MQ=60)
                          GAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGG                                                                                                                        >  SRR19973083.2136155/1‑150 (MQ=60)
                            AAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCT                                                                                                                      >  SRR19973083.29343/1‑150 (MQ=60)
                                GGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAG                                                                                                                  >  SRR19973083.4333609/1‑150 (MQ=60)
                                 GGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGA                                                                                                                 >  SRR19973083.4684940/1‑150 (MQ=60)
                                   AGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGT                                                                                                               >  SRR19973083.911985/1‑150 (MQ=60)
                                   AGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGT                                                                                                               >  SRR19973083.557065/1‑150 (MQ=60)
                                      GATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGAC                                                                                                            <  SRR19973083.775176/150‑1 (MQ=60)
                                      GATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGAC                                                                                                            <  SRR19973083.615448/150‑1 (MQ=60)
                                           ATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGG                                                                                                       >  SRR19973083.1014260/1‑150 (MQ=60)
                                           ATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGG                                                                                                       >  SRR19973083.5042914/1‑150 (MQ=60)
                                           ATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGG                                                                                                       >  SRR19973083.2102560/1‑150 (MQ=60)
                                            TTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGT                                                                                                      >  SRR19973083.2063695/1‑150 (MQ=60)
                                              ATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTAT                                                                                                    >  SRR19973083.4837755/1‑150 (MQ=60)
                                                CGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTT                                                                                                  <  SRR19973083.3310567/150‑1 (MQ=60)
                                                 GCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTG                                                                                                 >  SRR19973083.2311088/1‑150 (MQ=60)
                                                     CCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTT                                                                                             <  SRR19973083.1921213/150‑1 (MQ=60)
                                                      CTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTG                                                                                            <  SRR19973083.1187131/150‑1 (MQ=60)
                                                         GTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCT                                                                                         <  SRR19973083.2132497/150‑1 (MQ=60)
                                                          TCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTT                                                                                        >  SRR19973083.4662938/1‑150 (MQ=60)
                                                          TCTTAGAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTT                                                                                        <  SRR19973083.2041504/150‑1 (MQ=60)
                                                             TATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTAC                                                                                     <  SRR19973083.74870/150‑1 (MQ=60)
                                                             TATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTAC                                                                                     >  SRR19973083.3314521/1‑150 (MQ=60)
                                                              ATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTTACGCCGGTATTTGCTTTGCCTTTACG                                                                                    <  SRR19973083.2414177/150‑1 (MQ=60)
                                                                  CCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCA                                                                                <  SRR19973083.2140665/150‑1 (MQ=60)
                                                                       CAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAAC                                                                           >  SRR19973083.3170776/1‑150 (MQ=60)
                                                                       CAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAAC                                                                           >  SRR19973083.2377823/1‑150 (MQ=60)
                                                                        AGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACA                                                                          <  SRR19973083.591521/150‑1 (MQ=60)
                                                                         GACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAG                                                                         >  SRR19973083.1559377/1‑150 (MQ=60)
                                                                            ATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCAT                                                                      >  SRR19973083.4624490/1‑150 (MQ=60)
                                                                            ATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCAT                                                                      >  SRR19973083.2027758/1‑150 (MQ=60)
                                                                             TGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATA                                                                     <  SRR19973083.131776/150‑1 (MQ=60)
                                                                               GTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGA                                                                   <  SRR19973083.1018645/150‑1 (MQ=60)
                                                                                 TAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGA                                                                 <  SRR19973083.2554944/150‑1 (MQ=60)
                                                                                   GTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAAT                                                               <  SRR19973083.1768163/150‑1 (MQ=60)
                                                                                   GTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAAT                                                               <  SRR19973083.241564/150‑1 (MQ=60)
                                                                                      TTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAA                                                            <  SRR19973083.4414510/150‑1 (MQ=60)
                                                                                       TTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAG                                                           <  SRR19973083.3601243/150‑1 (MQ=60)
                                                                                       TTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAG                                                           <  SRR19973083.3601320/150‑1 (MQ=60)
                                                                                       TTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAG                                                           >  SRR19973083.2453178/1‑150 (MQ=60)
                                                                                       TTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAG                                                           <  SRR19973083.3601267/150‑1 (MQ=60)
                                                                                         CTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGG                                                         <  SRR19973083.5008493/150‑1 (MQ=60)
                                                                                         CTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGG                                                         <  SRR19973083.5008519/150‑1 (MQ=60)
                                                                                         CTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGG                                                         <  SRR19973083.5008713/150‑1 (MQ=60)
                                                                                          TTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGC                                                        >  SRR19973083.2526909/1‑150 (MQ=60)
                                                                                            GTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAG                                                      >  SRR19973083.4585775/1‑150 (MQ=60)
                                                                                            GTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAG                                                      >  SRR19973083.4932374/1‑150 (MQ=60)
                                                                                            GTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAG                                                      >  SRR19973083.4586100/1‑150 (MQ=60)
                                                                                              CATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGG                                                    <  SRR19973083.2905324/150‑1 (MQ=60)
                                                                                                       CACTATAGCGTGGTGAACGTGCACGCGGGTGCAGGACGCAGTTCCCTTTTGGTGCGTTCTGACTGTGGCTTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATACAGGGCAGGGCAAGTACCA                                           <  SRR19973083.2314234/150‑1 (MQ=60)
                                                                                                        AATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCAC                                          >  SRR19973083.2012687/1‑150 (MQ=60)
                                                                                                           ATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAG                                       >  SRR19973083.4952859/1‑150 (MQ=60)
                                                                                                              GTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAA                                    <  SRR19973083.2694814/150‑1 (MQ=60)
                                                                                                              GTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAA                                    <  SRR19973083.64561/150‑1 (MQ=60)
                                                                                                                GTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAAC                                  >  SRR19973083.4918670/1‑150 (MQ=60)
                                                                                                                GTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAAC                                  >  SRR19973083.4918749/1‑150 (MQ=60)
                                                                                                                GTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAAC                                  >  SRR19973083.4962146/1‑150 (MQ=60)
                                                                                                                   GTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAA                               <  SRR19973083.3140651/150‑1 (MQ=60)
                                                                                                                    TGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAAC                              >  SRR19973083.1837929/1‑150 (MQ=60)
                                                                                                                    TGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAAC                              <  SRR19973083.4582380/150‑1 (MQ=60)
                                                                                                                    TGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAAC                              >  SRR19973083.148272/1‑150 (MQ=60)
                                                                                                                    TGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAAC                              <  SRR19973083.1984400/150‑1 (MQ=60)
                                                                                                                    TGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAAC                              >  SRR19973083.1837910/1‑150 (MQ=60)
                                                                                                                     GAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACA                             >  SRR19973083.4322777/1‑150 (MQ=60)
                                                                                                                            CACGCGGGTGCAGGAAGAAGTTACCTTTTGTTGCGTTCTGACTGTTGATTGGCTCCAGTGTGACGCCGGTTTTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTA                      <  SRR19973083.1315785/150‑1 (MQ=60)
                                                                                                                                  GGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAA                <  SRR19973083.5091139/150‑1 (MQ=60)
                                                                                                                                       AGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATAC           <  SRR19973083.4120134/150‑1 (MQ=60)
                                                                                                                                       AGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATAC           >  SRR19973083.3550565/1‑150 (MQ=60)
                                                                                                                                       AGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATAC           >  SRR19973083.2167053/1‑150 (MQ=60)
                                                                                                                                       AGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATAC           <  SRR19973083.1011953/150‑1 (MQ=60)
                                                                                                                                       AGGAAGAAGTTACCTTTTGGAGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATAC           >  SRR19973083.2167114/1‑150 (MQ=60)
                                                                                                                                        GGAAGAAGTTTCCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACA          <  SRR19973083.471874/150‑1 (MQ=60)
                                                                                                                                         GAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACAT         >  SRR19973083.3413346/1‑150 (MQ=60)
                                                                                                                                          AAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATA        <  SRR19973083.1697529/150‑1 (MQ=60)
                                                                                                                                           AGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATAT       >  SRR19973083.346310/1‑150 (MQ=60)
                                                                                                                                           AGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATAT       >  SRR19973083.346258/1‑150 (MQ=60)
                                                                                                                                             AAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATATCG     <  SRR19973083.3228405/150‑1 (MQ=60)
                                                                                                                                                TTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATATCGGTA  >  SRR19973083.4511407/1‑150 (MQ=60)
                                                                                                                                                TTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATATCGGTA  <  SRR19973083.614866/150‑1 (MQ=60)
                                                                                                                                                TTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATATCGGTA  <  SRR19973083.3559373/150‑1 (MQ=60)
                                                                                                                                                 |                                                                                                                                                    
CGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATATCGGTA  >  CP009273/1563131‑1563424

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑