Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F488 I0 R1 1275 300.1 10328444 91.0% 9398884 150.0

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation freq annotation gene description
RA CP009273 1,563,276 G→T 100% H491N (CAC→AAC)  gadC ← glutamate:gamma‑aminobutyric acid antiporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP0092731,563,2760GT99.6% 841.8 / ‑4.9 254H491N (CAC→AAC) gadCglutamate:gamma‑aminobutyric acid antiporter
Reads supporting (aligned to +/- strand):  ref base G (0/1);  new base T (133/120);  total (133/121)
Fisher's exact test for biased strand distribution p-value = 4.76e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.88e-01
Rejected as polymorphism: E-value score below prediction cutoff.
Rejected as polymorphism: Frequency below/above cutoff threshold.
Rejected as polymorphism: Variant not supported by required number of reads on each strand.

Only 100 of 254 total aligned reads displayed.

CCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATATCGG  >  CP009273/1563129‑1563422
                                                                                                                                                   |                                                                                                                                                  
  cGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCt                                                                                                                                                <  2:3606562/150‑1 (MQ=255)
  cGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCt                                                                                                                                                >  1:4966576/1‑150 (MQ=255)
     tAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCtttt                                                                                                                                             >  2:1772292/1‑150 (MQ=255)
     tAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCtttt                                                                                                                                             >  1:919331/1‑150 (MQ=255)
           aaTACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCg                                                                                                                                       >  1:2199298/1‑150 (MQ=255)
             tACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGtt                                                                                                                                     <  2:4242289/150‑1 (MQ=255)
              aCGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATCACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTc                                                                                                                                    <  2:4420266/150‑1 (MQ=255)
              aCGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTc                                                                                                                                    <  1:3246632/150‑1 (MQ=255)
                  cAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGAc                                                                                                                                <  1:2270082/150‑1 (MQ=255)
                   agcaagcaCGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGTACGTGCACGCGGGTGCAGTAAGAAGTTACCTTTTGGTGCGTTCTGACt                                                                                                                               >  1:4790771/1‑150 (MQ=255)
                     caagcaCGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGt                                                                                                                             <  2:3577221/150‑1 (MQ=255)
                      aagcaCGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATCACCATTCAGACATGGTTAGTGTTACTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGtt                                                                                                                            <  2:3892234/150‑1 (MQ=255)
                       agcaCGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTg                                                                                                                           <  1:1204523/150‑1 (MQ=255)
                        gcaCGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGa                                                                                                                          >  2:3784128/1‑150 (MQ=255)
                          aCGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGACGATGTTACCTTTTGGTGCGTTCTGACTGTTGAtt                                                                                                                        <  2:1643964/150‑1 (MQ=255)
                          aCGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGAtt                                                                                                                        <  2:713187/150‑1 (MQ=255)
                              aaaaGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCt                                                                                                                    >  1:1965799/1‑150 (MQ=255)
                               aaaGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTc                                                                                                                   >  1:2151677/1‑150 (MQ=255)
                                     aGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGt                                                                                                             >  2:4034263/1‑150 (MQ=255)
                                       cGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGa                                                                                                           >  1:4603838/1‑150 (MQ=255)
                                         aTGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACg                                                                                                         <  1:3517565/150‑1 (MQ=255)
                                           gAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGcc                                                                                                       <  2:2484587/150‑1 (MQ=255)
                                              ttATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGt                                                                                                    <  1:1052639/150‑1 (MQ=255)
                                               tATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTa                                                                                                   >  2:4608678/1‑150 (MQ=255)
                                               tATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTa                                                                                                   >  2:2038207/1‑150 (MQ=255)
                                               tATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTa                                                                                                   <  1:4245717/150‑1 (MQ=255)
                                                  cGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTAttt                                                                                                >  2:4136531/1‑150 (MQ=255)
                                                   gCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTAtttg                                                                                               <  1:4294589/150‑1 (MQ=255)
                                                    cTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTAtttgc                                                                                              >  1:1860736/1‑150 (MQ=255)
                                                     tCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTAtttgct                                                                                             <  2:140043/150‑1 (MQ=255)
                                                      cccTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTAtttgctt                                                                                            >  1:2902917/1‑150 (MQ=255)
                                                      cccTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTAtttgctt                                                                                            <  2:4903929/150‑1 (MQ=255)
                                                      cacTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTAtttgctt                                                                                            >  2:5125171/3‑150 (MQ=255)
                                                       ccTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTAtttgcttt                                                                                           <  1:2650126/150‑1 (MQ=255)
                                                          tGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGcc                                                                                        >  1:3738380/1‑150 (MQ=255)
                                                           gTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCt                                                                                       >  2:2716564/1‑150 (MQ=255)
                                                             cTTATACCCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCttt                                                                                     <  1:3126619/150‑1 (MQ=255)
                                                             cTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCttt                                                                                     <  1:1368047/150‑1 (MQ=255)
                                                              ttATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTa                                                                                    <  1:1014306/150‑1 (MQ=255)
                                                              ttATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGCTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTa                                                                                    <  1:2628676/150‑1 (MQ=255)
                                                                 taACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGa                                                                                 <  1:2473453/150‑1 (MQ=255)
                                                                 taACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGa                                                                                 >  1:514762/1‑150 (MQ=255)
                                                                 taACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGa                                                                                 >  1:887389/1‑150 (MQ=255)
                                                                  aaCCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGAt                                                                                <  2:1430890/150‑1 (MQ=255)
                                                                  aaCCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGAt                                                                                <  1:492848/150‑1 (MQ=255)
                                                                   aCCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATc                                                                               <  1:4085719/150‑1 (MQ=255)
                                                                   aCCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATc                                                                               >  2:4735359/1‑150 (MQ=255)
                                                                   aCCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGAGTGGTGAACGTGAACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTTCTTTGCCTTTACGATc                                                                               >  2:3806937/1‑150 (MQ=255)
                                                                    ccATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCa                                                                              >  2:1699386/1‑150 (MQ=255)
                                                                     cATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCAt                                                                             >  1:1148989/1‑150 (MQ=255)
                                                                        tCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGaa                                                                          >  2:320387/1‑150 (MQ=255)
                                                                         cAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAAc                                                                         <  2:2157745/150‑1 (MQ=255)
                                                                          aGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACa                                                                        >  1:3432739/1‑150 (MQ=255)
                                                                              aTGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCAt                                                                    >  1:311868/1‑150 (MQ=255)
                                                                              aTGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCAt                                                                    >  1:2912602/1‑150 (MQ=255)
                                                                              aTGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCAt                                                                    >  2:4038975/1‑150 (MQ=255)
                                                                              aTGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCAt                                                                    >  1:4030415/1‑150 (MQ=255)
                                                                               tGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATa                                                                   >  2:2274959/1‑150 (MQ=255)
                                                                                ggTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATag                                                                  <  1:688585/150‑1 (MQ=255)
                                                                                ggTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATag                                                                  <  1:1772292/150‑1 (MQ=255)
                                                                                ggTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATag                                                                  >  2:3026416/1‑150 (MQ=255)
                                                                                 gTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATaga                                                                 >  2:1746968/1‑150 (MQ=255)
                                                                                 gTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGAATaga                                                                 >  2:1470133/1‑150 (MQ=255)
                                                                                 gTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGGTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTGACGATCATGAACAGCCTaga                                                                 >  2:1718494/1‑150 (MQ=255)
                                                                                   tAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAgaga                                                               >  2:3114998/1‑150 (MQ=255)
                                                                                    aGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGaa                                                              >  1:156683/1‑150 (MQ=255)
                                                                                    aGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGaa                                                              >  2:5024531/1‑150 (MQ=255)
                                                                                      tgtTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATa                                                            >  2:3493350/1‑150 (MQ=255)
                                                                                        tttCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATaaa                                                          <  2:3522318/150‑1 (MQ=255)
                                                                                             tGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAagggca                                                     <  1:1526336/150‑1 (MQ=255)
                                                                                              gTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAagggcag                                                    >  2:3706383/1‑150 (MQ=255)
                                                                                               tcattcatCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAagggcagg                                                   <  2:3625721/150‑1 (MQ=255)
                                                                                                cattcatCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTATCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAagggcaggg                                                  <  2:1174882/150‑1 (MQ=255)
                                                                                                   tcatcaCAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCaa                                               <  2:4794540/150‑1 (MQ=255)
                                                                                                     atcaCAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGt                                             <  1:505793/150‑1 (MQ=255)
                                                                                                           atatAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTaccacc                                       >  1:3525740/1‑150 (MQ=255)
                                                                                                            tataGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTAccacca                                      <  2:1871183/150‑1 (MQ=255)
                                                                                                             ataGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAg                                     >  2:3052100/1‑150 (MQ=255)
                                                                                                             ataGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGCTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAg                                     >  1:1337522/1‑150 (MQ=255)
                                                                                                               aGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGa                                   >  2:4452695/1‑150 (MQ=255)
                                                                                                                gtgtGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGaa                                  <  2:3536500/150‑1 (MQ=255)
                                                                                                                 tgtgGTGAACGTGCACGCGGGTGCAGGAAGAATTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGTGCAGGGCAAGTACCACCAGGaaa                                 <  1:625953/150‑1 (MQ=255)
                                                                                                                   tggtgAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACt                               >  2:2631482/1‑150 (MQ=255)
                                                                                                                       gAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTaaca                           >  2:2215186/1‑150 (MQ=255)
                                                                                                                             gCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGt                     <  2:2382175/150‑1 (MQ=255)
                                                                                                                              cACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTa                    <  1:4321575/150‑1 (MQ=255)
                                                                                                                                  cgGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTc                <  1:3786163/150‑1 (MQ=255)
                                                                                                                                  cgGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTc                >  2:675443/1‑150 (MQ=255)
                                                                                                                                    ggTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCaa              >  2:3906817/1‑150 (MQ=255)
                                                                                                                                     gTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAac             <  2:776183/150‑1 (MQ=255)
                                                                                                                                       gCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAacat           >  2:2111854/1‑150 (MQ=255)
                                                                                                                                        cAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAacata          <  1:1453822/150‑1 (MQ=255)
                                                                                                                                          ggAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAacataca        >  1:416636/1‑150 (MQ=255)
                                                                                                                                            aagaagTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAAcatacata      <  1:337294/150‑1 (MQ=255)
                                                                                                                                             agaagTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACatat     <  2:4332381/150‑1 (MQ=255)
                                                                                                                                             agaagTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACatat     >  2:2656197/1‑150 (MQ=255)
                                                                                                                                              gaagTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATATc    <  2:1965799/150‑1 (MQ=255)
                                                                                                                                              gaagTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATATc    <  1:1078276/150‑1 (MQ=255)
                                                                                                                                               aagTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATATCg   <  2:1117465/150‑1 (MQ=255)
                                                                                                                                                agTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATATCgg  <  1:4368028/150‑1 (MQ=255)
                                                                                                                                                   |                                                                                                                                                  
CCCGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATATCGG  >  CP009273/1563129‑1563422

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
CGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATATCGGTAG  >  CP009273/1563131‑1563425
                                                                                                                                                 |                                                                                                                                                     
CGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCT                                                                                                                                                   >  SRR19973063.4982699/1‑150 (MQ=60)
CGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCT                                                                                                                                                   >  SRR19973063.4982629/1‑150 (MQ=60)
 GTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACACTATAGTGTGGTGACCGTGCACGCGGGTGCAGGAAGAAGTTACCTT                                                                                                                                                  >  SRR19973063.2769861/1‑150 (MQ=60)
 GTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTT                                                                                                                                                  >  SRR19973063.2161956/1‑150 (MQ=60)
   TAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTT                                                                                                                                                >  SRR19973063.922044/1‑150 (MQ=60)
        CAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGC                                                                                                                                           <  SRR19973063.3335965/150‑1 (MQ=60)
         AATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCG                                                                                                                                          >  SRR19973063.2206218/1‑150 (MQ=60)
            ACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTC                                                                                                                                       <  SRR19973063.3257056/150‑1 (MQ=60)
              GACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTG                                                                                                                                     >  SRR19973063.387061/1‑150 (MQ=60)
               ACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGA                                                                                                                                    <  SRR19973063.4053897/150‑1 (MQ=60)
                CAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGAC                                                                                                                                   <  SRR19973063.2277328/150‑1 (MQ=60)
                 AGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGTACGTGCACGCGGGTGCAGTAAGAAGTTACCTTTTGGTGCGTTCTGACT                                                                                                                                  >  SRR19973063.4806079/1‑150 (MQ=60)
                 AGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACT                                                                                                                                  >  SRR19973063.896567/1‑150 (MQ=60)
                     AGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTG                                                                                                                              <  SRR19973063.3763738/150‑1 (MQ=60)
                     AGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTG                                                                                                                              <  SRR19973063.1208138/150‑1 (MQ=60)
                            AAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCT                                                                                                                       >  SRR19973063.1971952/1‑150 (MQ=60)
                             AAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTC                                                                                                                      >  SRR19973063.2158416/1‑150 (MQ=60)
                              AAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCC                                                                                                                     >  SRR19973063.4482715/1‑150 (MQ=60)
                                   AGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGT                                                                                                                <  SRR19973063.3505726/150‑1 (MQ=60)
                                   AGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGT                                                                                                                <  SRR19973063.3505727/150‑1 (MQ=60)
                                     CGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGA                                                                                                              >  SRR19973063.4618563/1‑150 (MQ=60)
                                       ATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACG                                                                                                            <  SRR19973063.3528819/150‑1 (MQ=60)
                                         GAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCTCGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCC                                                                                                          <  SRR19973063.3538827/150‑1 (MQ=60)
                                            TTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGT                                                                                                       <  SRR19973063.1055827/150‑1 (MQ=60)
                                             TATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTA                                                                                                      <  SRR19973063.4259249/150‑1 (MQ=60)
                                               TCGCTCCCTTGTCTTCTAACCAGTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATT                                                                                                    <  SRR19973063.806207/150‑1 (MQ=60)
                                                 GCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTG                                                                                                  <  SRR19973063.4308235/150‑1 (MQ=60)
                                                 GCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTG                                                                                                  <  SRR19973063.466549/150‑1 (MQ=60)
                                                  CTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGC                                                                                                 >  SRR19973063.1866546/1‑150 (MQ=60)
                                                  CTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAAAGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGC                                                                                                 >  SRR19973063.1866617/1‑150 (MQ=60)
                                                    CCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTT                                                                                               <  SRR19973063.3425864/150‑1 (MQ=60)
                                                    CCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTT                                                                                               <  SRR19973063.3425745/150‑1 (MQ=60)
                                                    CCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTT                                                                                               >  SRR19973063.2912181/1‑150 (MQ=60)
                                                     CCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTT                                                                                              <  SRR19973063.2658600/150‑1 (MQ=60)
                                                        TGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCC                                                                                           >  SRR19973063.3750318/1‑150 (MQ=60)
                                                           CTTATACCCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTT                                                                                        <  SRR19973063.3136685/150‑1 (MQ=60)
                                                           CTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTT                                                                                        <  SRR19973063.1372116/150‑1 (MQ=60)
                                                           CTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTT                                                                                        <  SRR19973063.1147927/150‑1 (MQ=60)
                                                           CTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTT                                                                                        <  SRR19973063.1372170/150‑1 (MQ=60)
                                                            TTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTA                                                                                       <  SRR19973063.1017363/150‑1 (MQ=60)
                                                            TTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTA                                                                                       >  SRR19973063.1934252/1‑150 (MQ=60)
                                                            TTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTA                                                                                       <  SRR19973063.856472/150‑1 (MQ=60)
                                                            TTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGCTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTA                                                                                       <  SRR19973063.2637058/150‑1 (MQ=60)
                                                             TATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTAC                                                                                      <  SRR19973063.1782124/150‑1 (MQ=60)
                                                              ATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACG                                                                                     <  SRR19973063.264120/150‑1 (MQ=60)
                                                              ATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACG                                                                                     <  SRR19973063.3256334/150‑1 (MQ=60)
                                                              ATAACCATTCAGACATGGTTAGTGTTTCTTGTAATTCATCACAATATAGTGTGGTGAACGTGCACGCGGTTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACG                                                                                     <  SRR19973063.661649/150‑1 (MQ=60)
                                                               TAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGA                                                                                    >  SRR19973063.516265/1‑150 (MQ=60)
                                                               TAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGA                                                                                    <  SRR19973063.2481358/150‑1 (MQ=60)
                                                               TAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGA                                                                                    >  SRR19973063.4767101/1‑150 (MQ=60)
                                                               TAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGA                                                                                    >  SRR19973063.889954/1‑150 (MQ=60)
                                                                AACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGAT                                                                                   <  SRR19973063.494263/150‑1 (MQ=60)
                                                                AACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGAT                                                                                   <  SRR19973063.360751/150‑1 (MQ=60)
                                                                 ACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATC                                                                                  <  SRR19973063.4098806/150‑1 (MQ=60)
                                                                   CNTTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCAT                                                                                >  SRR19973063.1356283/1‑150 (MQ=60)
                                                                   CATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCAT                                                                                >  SRR19973063.1152462/1‑150 (MQ=60)
                                                                    ATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATG                                                                               >  SRR19973063.4223801/1‑150 (MQ=60)
                                                                        AGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACA                                                                           >  SRR19973063.3443659/1‑150 (MQ=60)
                                                                        AGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACA                                                                           <  SRR19973063.5035638/150‑1 (MQ=60)
                                                                        AGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACA                                                                           >  SRR19973063.2228133/1‑150 (MQ=60)
                                                                        AGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACA                                                                           <  SRR19973063.2886475/150‑1 (MQ=60)
                                                                         GACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAG                                                                          >  SRR19973063.2028696/1‑150 (MQ=60)
                                                                         GACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAG                                                                          >  SRR19973063.298075/1‑150 (MQ=60)
                                                                            ATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCAT                                                                       >  SRR19973063.4043334/1‑150 (MQ=60)
                                                                            ATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCAT                                                                       >  SRR19973063.313015/1‑150 (MQ=60)
                                                                            ATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCAT                                                                       >  SRR19973063.312779/1‑150 (MQ=60)
                                                                            ATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCAT                                                                       >  SRR19973063.2921893/1‑150 (MQ=60)
                                                                             TGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATA                                                                      <  SRR19973063.4327472/150‑1 (MQ=60)
                                                                              GGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAG                                                                     <  SRR19973063.1777751/150‑1 (MQ=60)
                                                                              GGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAG                                                                     <  SRR19973063.690604/150‑1 (MQ=60)
                                                                                  AGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAA                                                                 >  SRR19973063.157168/1‑150 (MQ=60)
                                                                                     GTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAA                                                              <  SRR19973063.1249317/150‑1 (MQ=60)
                                                                                         CTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGG                                                          <  SRR19973063.4949559/150‑1 (MQ=60)
                                                                                           TGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCA                                                        <  SRR19973063.1531011/150‑1 (MQ=60)
                                                                                           TGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCA                                                        <  SRR19973063.4298312/150‑1 (MQ=60)
                                                                                                 TCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAA                                                  >  SRR19973063.1687662/1‑150 (MQ=60)
                                                                                                   ATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGT                                                <  SRR19973063.507259/150‑1 (MQ=60)
                                                                                                     CACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTAC                                              <  SRR19973063.1043714/150‑1 (MQ=60)
                                                                                                       CAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCA                                            <  SRR19973063.626348/150‑1 (MQ=60)
                                                                                                        AATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCAC                                           >  SRR19973063.4290706/1‑150 (MQ=60)
                                                                                                        AATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCAC                                           >  SRR19973063.2923671/1‑150 (MQ=60)
                                                                                                         ATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACC                                          >  SRR19973063.151416/1‑150 (MQ=60)
                                                                                                         ATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACC                                          >  SRR19973063.3537029/1‑150 (MQ=60)
                                                                                                         ATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACC                                          >  SRR19973063.1616026/1‑150 (MQ=60)
                                                                                                         ATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACC                                          >  SRR19973063.2581155/1‑150 (MQ=60)
                                                                                                           ATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAG                                        >  SRR19973063.1192125/1‑150 (MQ=60)
                                                                                                           ATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAG                                        >  SRR19973063.1472057/1‑150 (MQ=60)
                                                                                                           ATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGCTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAG                                        >  SRR19973063.1341531/1‑150 (MQ=60)
                                                                                                            TAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGG                                       >  SRR19973063.2098104/1‑150 (MQ=60)
                                                                                                             AGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGA                                      >  SRR19973063.4919175/1‑150 (MQ=60)
                                                                                                               TGTGGTGAACGTGCACGCGGGTGCAGGAAGAATTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGTGCAGGGCAAGTACCACCAGGAAA                                    <  SRR19973063.627794/150‑1 (MQ=60)
                                                                                                                 TGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACT                                  >  SRR19973063.1531407/1‑150 (MQ=60)
                                                                                                                 TGGTGAACGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACT                                  >  SRR19973063.2544023/1‑150 (MQ=60)
                                                                                                                        CGTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACC                           <  SRR19973063.1169062/150‑1 (MQ=60)
                                                                                                                         GTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCA                          <  SRR19973063.2916794/150‑1 (MQ=60)
                                                                                                                         GTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCA                          <  SRR19973063.549869/150‑1 (MQ=60)
                                                                                                                         GTGCACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCA                          <  SRR19973063.2275688/150‑1 (MQ=60)
                                                                                                                            CACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATTAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTA                       <  SRR19973063.4815037/150‑1 (MQ=60)
                                                                                                                            CACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTA                       <  SRR19973063.4335323/150‑1 (MQ=60)
                                                                                                                             ACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAA                      >  SRR19973063.4365420/1‑150 (MQ=60)
                                                                                                                             ACGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAA                      >  SRR19973063.4365349/1‑150 (MQ=60)
                                                                                                                              CGCGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAAT                     <  SRR19973063.3534240/150‑1 (MQ=60)
                                                                                                                                CGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTC                   <  SRR19973063.2342959/150‑1 (MQ=60)
                                                                                                                                CGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTC                   >  SRR19973063.4116024/1‑150 (MQ=60)
                                                                                                                                CGGGTGCAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTC                   <  SRR19973063.3798242/150‑1 (MQ=60)
                                                                                                                                 GGGTGCAGGAAGAAGTTACTTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCA                  >  SRR19973063.3175421/1‑150 (MQ=60)
                                                                                                                                      CAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATA             <  SRR19973063.1458256/150‑1 (MQ=60)
                                                                                                                                      CAGGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGATTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATA             <  SRR19973063.1458118/150‑1 (MQ=60)
                                                                                                                                        GGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACA           >  SRR19973063.417719/1‑150 (MQ=60)
                                                                                                                                        GGAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACA           >  SRR19973063.417805/1‑150 (MQ=60)
                                                                                                                                         GAAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACAT          <  SRR19973063.3245536/150‑1 (MQ=60)
                                                                                                                                          AAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATA         <  SRR19973063.338271/150‑1 (MQ=60)
                                                                                                                                          AAGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATA         <  SRR19973063.5115670/150‑1 (MQ=60)
                                                                                                                                           AGAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATAT        >  SRR19973063.3985097/1‑150 (MQ=60)
                                                                                                                                            GAAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATATC       <  SRR19973063.1081555/150‑1 (MQ=60)
                                                                                                                                             AAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATATCG      >  SRR19973063.1077926/1‑150 (MQ=60)
                                                                                                                                             AAGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATATCG      >  SRR19973063.1077805/1‑150 (MQ=60)
                                                                                                                                              AGTTACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATATCGG     <  SRR19973063.4382002/150‑1 (MQ=60)
                                                                                                                                                 TACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATATCGGTAG  <  SRR19973063.4162157/150‑1 (MQ=60)
                                                                                                                                                 |                                                                                                                                                     
CGTTAAGTCAATACGACAGCAAGCACGAAAAAGGGAGCGATGAATTATCGCTCCCTTGTCTTATAACCATTCAGACATGGTTAGTGTTTCTTGTCATTCATCACAATATAGTGTGGTGAACGTGCACGCGGGTGCAGGAAGAAGTGACCTTTTGGTGCGTTCTGACTGTTGATTGGCTCCAGAGTGACGCCGGTATTTGCTTTGCCTTTACGATCATGAACAGCATAGAGAATAAAGGGCAGGGCAAGTACCACCAGGAAACTAACAACCAGTAATTCAACATACATATCGGTAG  >  CP009273/1563131‑1563425

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑