Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 26 102.0 3326124 78.0% 2594376 98.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA CP002094 305,497 A→G V365V (GTT→GTC)  LLNZ_01670 ← Nisin biosynthesis two‑component system, sensor histidine kinase NisK

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*CP002094305,4970AG100.0% 269.6 / NA 65V365V (GTT→GTC) LLNZ_01670Nisin biosynthesis two‑component system, sensor histidine kinase NisK
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (36/29);  total (36/29)

TTCCAGCATTTTTTTTTGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTAACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAATGTATTTCCGTAAAAAGTGG  >  CP002094/305403‑305591
                                                                                              |                                                                                              
ttCCAGCATTTTTTTTTGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAactgacta                                                                                            <  2:980752/99‑1 (MQ=255)
ttCCAGCATTTTTTTTTGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAactgacta                                                                                            <  2:835113/99‑1 (MQ=255)
ttCCAGCATTTTTTTTTGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAactgacta                                                                                            <  1:492589/99‑1 (MQ=255)
ttCCAGCATTTTTTTTTGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAactgacta                                                                                            <  2:1269238/99‑1 (MQ=255)
  ccAGCATTTTTTTTTGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTAtt                                                                                          <  1:61404/99‑1 (MQ=255)
     gCATTTTTTTTTGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAACATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTAttttt                                                                                       >  1:1583104/1‑99 (MQ=255)
      cATTTTTTTTTGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTAtttttt                                                                                      <  2:383956/99‑1 (MQ=255)
      cATTTTTTTTTGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTAtttttt                                                                                      >  2:1486616/1‑99 (MQ=255)
      cATTTTTTTTTGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTAtttttt                                                                                      <  2:1187625/99‑1 (MQ=255)
      cATTTTTTTTTGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTAtttttt                                                                                      <  1:1225932/99‑1 (MQ=255)
       aTTTTTTTTTGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTc                                                                                     >  2:1121064/1‑99 (MQ=255)
       aTTTTTTTTTGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTc                                                                                     >  2:1021577/1‑99 (MQ=255)
        tttttttttGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCa                                                                                    <  2:783770/99‑1 (MQ=255)
        tttttttttGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCa                                                                                    <  1:1580904/99‑1 (MQ=255)
         ttttttttGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCAc                                                                                   <  2:120212/99‑1 (MQ=255)
         ttttttttGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCAc                                                                                   <  1:980012/99‑1 (MQ=255)
          tttttttGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTT‑ATCATCATAAATACTCAAACTGACTATTTTTTCAccc                                                                                 >  1:810915/1‑99 (MQ=255)
           ttttttGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCAccc                                                                                 >  1:624791/1‑99 (MQ=255)
           ttttttGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCAccc                                                                                 <  2:1148212/99‑1 (MQ=255)
                tGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATTCTCAAACTGACTATTTTTTCTCCCTCttt                                                                            <  2:362106/99‑1 (MQ=255)
                  cTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAg                                                                          <  1:462022/99‑1 (MQ=255)
                   ttGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGc                                                                         <  2:80855/99‑1 (MQ=255)
                       tCAGAAAAAAGATGACCCATTTTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATAc                                                                     <  1:1369853/99‑1 (MQ=255)
                             aaaGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAg                                                               >  2:593265/1‑99 (MQ=255)
                               aGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGc                                                             >  1:1225425/1‑99 (MQ=255)
                               aGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGc                                                             >  1:986551/1‑99 (MQ=255)
                                 gATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCAt                                                           >  1:728841/1‑99 (MQ=255)
                                    gACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTaa                                                        <  2:1518665/99‑1 (MQ=255)
                                     aCCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAAc                                                       >  2:211435/1‑99 (MQ=255)
                                     aCCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAAc                                                       <  2:1318276/99‑1 (MQ=255)
                                     aCCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAAc                                                       >  1:271101/1‑99 (MQ=255)
                                        attattCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACaaa                                                    >  1:791916/1‑99 (MQ=255)
                                         ttattCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAg                                                   >  2:51034/1‑99 (MQ=255)
                                            ttCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGata                                                >  1:456969/1‑99 (MQ=255)
                                             tCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGatat                                               >  1:752520/1‑99 (MQ=255)
                                                 gATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGAt                                           >  1:864857/1‑99 (MQ=255)
                                                  aTTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATa                                          <  2:1634399/99‑1 (MQ=255)
                                                   tttCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATaa                                         >  2:964498/1‑99 (MQ=255)
                                                    ttCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAg                                        <  1:220349/99‑1 (MQ=255)
                                                    ttCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAg                                        <  2:483784/99‑1 (MQ=255)
                                                    ttCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAg                                        <  1:67641/99‑1 (MQ=255)
                                                        aaaTAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCt                                    <  2:1451568/99‑1 (MQ=255)
                                                        aaaTAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCt                                    >  1:637946/1‑99 (MQ=255)
                                                         aaTAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTc                                   >  2:1573438/1‑99 (MQ=255)
                                                          aTAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCg                                  >  2:1183795/1‑99 (MQ=255)
                                                          aTAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCg                                  >  2:1475497/1‑99 (MQ=255)
                                                              agaTATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACtt                              <  2:624791/99‑1 (MQ=255)
                                                              agaTATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACtt                              >  2:658270/1‑99 (MQ=255)
                                                              agaTATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACtt                              <  2:264886/99‑1 (MQ=255)
                                                              agaTATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACtt                              >  2:877733/1‑99 (MQ=255)
                                                               gaTATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTa                             >  1:1410529/1‑99 (MQ=255)
                                                                atatTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTaa                            <  2:1115175/99‑1 (MQ=255)
                                                                  atTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAg                          <  2:399117/99‑1 (MQ=255)
                                                                   tttttCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGc                         >  1:1216059/1‑99 (MQ=255)
                                                                   tttttCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGc                         >  1:573600/1‑99 (MQ=255)
                                                                    ttttCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCt                        <  1:958256/99‑1 (MQ=255)
                                                                       tcatcatcaTAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAAt                     >  1:1554757/1‑99 (MQ=255)
                                                                          tcatcatAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAATGTa                  >  2:567264/1‑99 (MQ=255)
                                                                           catcatAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAATGTAt                 <  1:11577/99‑1 (MQ=255)
                                                                            atcatAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAATGTAtt                <  2:311256/99‑1 (MQ=255)
                                                                            atcatAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAATGTAtt                >  2:725843/1‑99 (MQ=255)
                                                                             tcatAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAATGTAttt               <  2:553292/99‑1 (MQ=255)
                                                                             tcatAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAATGTAttt               <  1:1256215/99‑1 (MQ=255)
                                                                             tcatAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAATGTAttt               >  1:811505/1‑99 (MQ=255)
                                                                                tAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAATGTATTTCCg            >  1:401092/1‑99 (MQ=255)
                                                                                  aaTACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAATGTATTTCCGTa          >  2:408874/1‑99 (MQ=255)
                                                                                  aaTACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAATGTATTTCCGTa          >  2:57553/1‑99 (MQ=255)
                                                                                  aaTACTCAAACTGACTATTTTTTCACCCTCTTTAACATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAATGTATTTCCGTa          >  2:130841/1‑99 (MQ=255)
                                                                                      cTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAATGTATTTCCGTaaaaa      >  1:297329/1‑99 (MQ=255)
                                                                                          aactgactATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAATGTATTTCCGTAAAAAGTgg  >  1:608910/1‑99 (MQ=255)
                                                                                          aactgactATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAATGTATTTCCGTAAAAAGTgg  >  1:593837/1‑99 (MQ=255)
                                                                                          aactgactATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAATGTATTTCCGTAAAAAGTgg  >  2:633798/1‑99 (MQ=255)
                                                                                              |                                                                                              
TTCCAGCATTTTTTTTTGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTAACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAATGTATTTCCGTAAAAAGTGG  >  CP002094/305403‑305591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑

GATK/CNVnator alignment

BRESEQ :: bam2aln output
TAGTTTTCCAGCATTTTTTTTTGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTAACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAATGTATTTCCGTAAAAAGTGG  >  CP002094/305398‑305591
                                                                                                   |                                                                                              
TAGTTTTCCAGCATTTTTTTTTGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTG                                                                                                >  SRR7892090.1460126/1‑100 (MQ=60)
  GTTTTCCAGCATTTTTTTTTGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGAC                                                                                              <  SRR7892090.1140545/100‑1 (MQ=60)
     TTCCAGCATTTTTTTTTGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTAT                                                                                           <  SRR7892090.497034/100‑1 (MQ=60)
       CCAGCATTTTTTTTTGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTT                                                                                         <  SRR7892090.61955/100‑1 (MQ=60)
         AGCATTTTTTTTTGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAACATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTT                                                                                       >  SRR7892090.1592950/1‑100 (MQ=60)
           CATTTTTTTTTGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTC                                                                                     <  SRR7892090.1233692/100‑1 (MQ=60)
             TTTTTTTTTGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCAC                                                                                   <  SRR7892090.1590720/100‑1 (MQ=60)
              TTTTTTTTGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACC                                                                                  <  SRR7892090.987082/100‑1 (MQ=60)
              TTTTTTTTGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTT‑ATCATCATAAATACTCAAACTGACTATTTTTTCACCC                                                                                 >  SRR7892090.817348/1‑100 (MQ=60)
               TTTTTTTGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCC                                                                                 >  SRR7892090.630172/1‑100 (MQ=60)
                       CTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGC                                                                         <  SRR7892090.466278/100‑1 (MQ=60)
                            TCAGAAAAAAGATGACCCATTTTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACT                                                                    <  SRR7892090.1377902/100‑1 (MQ=60)
                                   AAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGC                                                             >  SRR7892090.993631/1‑100 (MQ=60)
                                   AAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGC                                                             >  SRR7892090.1233182/1‑100 (MQ=60)
                                     GGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCAT                                                           >  SRR7892090.734878/1‑100 (MQ=60)
                                         GACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAAC                                                       >  SRR7892090.273708/1‑100 (MQ=60)
                                           CCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTttcccccctttttttttttaaaaaaaatataaa                                                     >  SRR7892090.550677/1‑67 (MQ=60)
                                            CATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAA                                                    >  SRR7892090.798225/1‑100 (MQ=60)
                                                ATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATA                                                >  SRR7892090.461185/1‑100 (MQ=60)
                                                 TTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATAT                                               >  SRR7892090.758627/1‑100 (MQ=60)
                                                     AGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGAT                                           >  SRR7892090.871545/1‑100 (MQ=60)
                                                         TTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGT                                       <  SRR7892090.222414/100‑1 (MQ=60)
                                                         TTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGT                                       <  SRR7892090.68236/100‑1 (MQ=60)
                                                            AAAATAGAGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCT                                    >  SRR7892090.643365/1‑100 (MQ=60)
                                                                   AGATATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTA                             >  SRR7892090.1418646/1‑100 (MQ=60)
                                                                       ATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGC                         >  SRR7892090.578762/1‑100 (MQ=60)
                                                                       ATTTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGC                         >  SRR7892090.1223798/1‑100 (MQ=60)
                                                                         TTTTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTA                       <  SRR7892090.965268/100‑1 (MQ=60)
                                                                           TTCATCATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAAT                     >  SRR7892090.1564313/1‑100 (MQ=60)
                                                                                CATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAATGTATT                <  SRR7892090.11702/100‑1 (MQ=60)
                                                                                 ATCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAATGTATTT               >  SRR7892090.817938/1‑100 (MQ=60)
                                                                                  TCATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAATGTATTTC              <  SRR7892090.1264044/100‑1 (MQ=60)
                                                                                    ATAAATACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAATGTATTTCCG            >  SRR7892090.405012/1‑100 (MQ=60)
                                                                                          ACTCAAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAATGTATTTCCGTAAAAA      >  SRR7892090.300187/1‑100 (MQ=60)
                                                                                              AAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAATGTATTTCCGTAAAAAGTGG  >  SRR7892090.614217/1‑100 (MQ=60)
                                                                                              AAACTGACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAATGTATTTCCGTAAAAAGTGG  >  SRR7892090.599109/1‑100 (MQ=60)
                                                                                                   |                                                                                              
TAGTTTTCCAGCATTTTTTTTTGCTTGTTCAGAAAAAGGATGACCATTATTCCAGATTTCAAAATAGAGATATTTTTCATCATCATAAATACTCAAACTAACTATTTTTTCACCCTCTTTAGCATACTGACAGGCATTAACAAAGATATTGATAAGTGCTCGACTTAAAGCTAATGTATTTCCGTAAAAAGTGG  >  CP002094/305398‑305591

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑