breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 305,497 A→G V365V (GTT→GTC LLNZ_01670 ← Nisin biosynthesis two‑component system, sensor histidine kinase NisK
RA 305,693 C→A R300I (AGA→ATA)  LLNZ_01670 ← Nisin biosynthesis two‑component system, sensor histidine kinase NisK
RA 305,770 T→C L274L (TTA→TTG LLNZ_01670 ← Nisin biosynthesis two‑component system, sensor histidine kinase NisK
RA 305,914 C→T K226K (AAG→AAA LLNZ_01670 ← Nisin biosynthesis two‑component system, sensor histidine kinase NisK
RA 306,199 T→A P131P (CCA→CCT LLNZ_01670 ← Nisin biosynthesis two‑component system, sensor histidine kinase NisK
RA 306,367 C→A M75I (ATG→ATT LLNZ_01670 ← Nisin biosynthesis two‑component system, sensor histidine kinase NisK
RA 306,674 C→T E199E (GAG→GAA LLNZ_01675 ← Nisin biosynthesis two‑component system, response regulator NisR
RA 308,336 C→G G142R (GGT→CGT)  LLNZ_01685 ← multidrug‑efflux transporter protein
MC JC 308,346 (AGC)3→2 coding (412‑414/1080 nt) LLNZ_01685 ← multidrug‑efflux transporter protein
RA 444,791 (T)8→9 intergenic (+275/‑718) LLNZ_02320 → / → glmM hypothetical protein/phosphoglucosamine mutase
RA 637,425 (A)7→6 coding (297/1164 nt) LLNZ_03315 ← hypothetical protein
RA 771,661 A→G I23V (ATC→GTC)  aroD → 3‑dehydroquinate dehydratase
RA 1,095,659 (A)7→6 intergenic (+76/‑149) LLNZ_05830 → / → LLNZ_05835 hypothetical protein/hypothetical protein
RA 1,191,576 C→T Q309* (CAA→TAA)  LLNZ_06295 → D‑alanine transfer protein DltD
RA 1,224,391 2 bp→AG intergenic (+244/+299) LLNZ_06440 → / ← LLNZ_06445 hypothetical protein/hypothetical protein
RA 1,238,479 C→A D34E (GAC→GAA LLNZ_06550 → hypothetical protein
RA 1,319,382 (A)7→6 coding (34/348 nt) LLNZ_06950 → hypothetical protein
RA 1,875,806 +TT coding (566/762 nt) LLNZ_09765 ← phosphate transporter ATP‑binding protein
RA 1,973,400 G→T A183S (GCC→TCC)  LLNZ_10250 → putative neutral endopeptidase O2
RA 2,183,875 (A)5→4 coding (399/1161 nt) LLNZ_11440 ← hypothetical protein
RA 2,396,538 T→C D73G (GAC→GGC)  LLNZ_12635 ← hypothetical protein

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ CP002094 670605 672212–671033 429–1608 46 [45] [45] 46 LLNZ_03505–LLNZ_03520 LLNZ_03505,LLNZ_03510,LLNZ_03515,LLNZ_03520
* * ÷ CP002094 673606–675687 676703–675714 28–3098 46 [44] [45] 48 LLNZ_03540–LLNZ_03555 LLNZ_03540,LLNZ_03545,LLNZ_03550,LLNZ_03555

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? CP002094 705776 =NA (NA)109 (1.090) 48/196 1.7 NA coding (127/261 nt) LLNZ_03755 IS981 transposase A
?CP002094 = 797569 NA (NA)intergenic (+88/‑293) LLNZ_04245/LLNZ_04250 putative transposase helper protein for IS712H/terminase small subunit
* ? CP002094 1041791 =5 (0.050)93 (0.940) 77/194 0.1 95.0% coding (124/570 nt) LLNZ_05610 hypothetical protein
?CP002094 1204449 = NA (NA)intergenic (+84/+53) LLNZ_06340/LLNZ_06345 transposase/transposase
* ? CP002094 = 10417951 (0.010)96 (0.970) 65/194 0.5 99.0% coding (128/570 nt) LLNZ_05610 hypothetical protein
?CP002094 = 1205670 NA (NA)intergenic (‑57/‑15) LLNZ_06350/LLNZ_06355 hypothetical protein/hypothetical protein
* ? CP002094 = 2135803NA (NA)57 (0.830)
+30 bp
30/136 2.0 NA noncoding (865/1556 nt) LLNZ_r13403 16S ribosomal RNA
?CP002094 2135833 = NA (NA)noncoding (835/1556 nt) LLNZ_r13403 16S ribosomal RNA
* ? CP002094 = 248265516 (0.160)74 (0.870) 56/168 0.5 84.2% coding (48/375 nt) LLNZ_13030 putative alkylphosphonate uptake protein
?CP002094 2493241 = 14 (0.160)intergenic (+476/+84) LLNZ_13110/LLNZ_13115 hypothetical protein/glyceraldehyde 3‑phosphate dehydrogenase