Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
152 |
24.9 |
2083062 |
91.6% |
1908084 |
109.2 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
1,841,200 |
+G |
intergenic (‑2736/‑2680) |
h16_A1684 ← / → ycaC |
conserved hypothetical protein/YcaC related amidohydrolase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 1,841,197 | 1 | . | G | 97.0%
| 122.6
/ ‑3.3
| 33 | intergenic (‑2733/‑2683) | h16_A1684/ycaC | conserved hypothetical protein/YcaC related amidohydrolase |
| Reads supporting (aligned to +/- strand): ref base . (1/0); new base G (19/13); total (20/13) |
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 |
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 |
GAAGAAGCGTCGGCGGCAATGCGCCCAGCATCCGCCCTCGATGATCCGGCCGGTACGGCAAAGCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTT‑CA‑GGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAGGTAGCCCCATAGATGGGCCTGCCGCGTGGT > AM260479/1841073‑1841323
|
gaagaaGCGTCGGCGGCAATGCGCCCAGCATCCGCCCTCGATGATCCGGCCGGTACGGCAAAGCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTT‑CA‑ggggatgg < 2:1019471/133‑6 (MQ=255)
agCGTCGGCGGCAATGCGCCCAGCATCCGCCCTCGATGATCCGGCCGGTACGGCAAAGCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTT‑CAGGGGATGGGCGCCg > 1:536437/1‑134 (MQ=255)
aTCCGCCCTCGATGATCCGGCCGGTACGGCAAAGCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTT‑CAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAAc < 1:85094/134‑1 (MQ=255)
ccTCGATGATCCGGCCGGTACGGCAAAGCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTT‑CAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCAcc > 1:535272/1‑134 (MQ=255)
ccggTACGGCAAAGCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTT‑CAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTc > 1:46957/1‑134 (MQ=255)
cGGCAAAGCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTT‑CAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCa > 2:424530/1‑134 (MQ=255)
gCAAAGCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTT‑CAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCgctg < 2:812980/116‑1 (MQ=255)
gCAAAGCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTT‑CAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCgctg > 1:812980/1‑116 (MQ=255)
cATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTT‑CAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTtc > 2:598068/1‑134 (MQ=255)
tCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCCG‑GGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCCGGTGGTCGACCCATAAACCGTtctc > 2:913999/1‑134 (MQ=255)
tCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTT‑CAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATaa > 2:2734/1‑125 (MQ=255)
tCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTT‑CAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATaa < 1:2734/125‑1 (MQ=255)
tCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTT‑CAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTtctc > 1:620309/1‑134 (MQ=255)
gCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTT‑CAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGcc > 1:273323/1‑134 (MQ=255)
tGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTT‑CAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGc > 2:710800/1‑134 (MQ=255)
cAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTT‑CAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGcc < 2:536437/134‑1 (MQ=255)
gccgccTGCAGGTAGCCATGATAGTCGCGCAGGAAGTT‑CAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCAc < 2:535272/134‑1 (MQ=255)
gccTGCAGGTAGCCATGATAGTCGCGCAGGAAGTT‑CAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAg < 1:598068/134‑1 (MQ=255)
gccTGCAGGTAGCCATGATAGTCGCGCAGGAAGTT‑CAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAg < 1:424530/134‑1 (MQ=255)
cTGCAGGTAGCCATGATAGTCGCGCAGGAAGTT‑CAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACg < 1:145232/130‑1 (MQ=255)
cTGCAGGTAGCCATGATAGTCGCGCAGGAAGTT‑CAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACg > 2:145232/1‑130 (MQ=255)
cTGCAGGTAGCCATGATAGTCGCGCAGGAAGTT‑CAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAgg > 1:935244/1‑133 (MQ=255)
cTGCAGGTAGCCATGATAGTCGCGCAGGAAGTT‑CAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAgg > 1:649575/1‑133 (MQ=255)
tGCAGGTAGCCATGATAGTCGCGCAGGAAGTT‑CAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAgg > 2:917509/1‑132 (MQ=255)
cAGGTAGCCATGATAGTCGCGCAGGAAGTT‑CAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAg < 2:428856/129‑1 (MQ=255)
cAGGTAGCCATGATAGTCGCGCAGGAAGTT‑CAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAg > 1:428856/1‑129 (MQ=255)
ggTAGCCATGATAGTCGCGCAGGAAGTT‑CAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCg > 2:592916/1‑89 (MQ=255)
ggTAGCCATGATAGTCGCGCAGGAAGTT‑CAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCg < 1:592916/89‑1 (MQ=255)
tAGCCATGATAGTCGCGCAGGAAGTT‑CAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAGGTAGCCCCa < 2:46957/134‑1 (MQ=255)
gATAGTCGCGCAGGAAGTT‑CAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAGGTAGCCCCATAGATgg > 2:350326/1‑134 (MQ=255)
aGTCGCGCAGGAAGTT‑CAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCtggtg > 2:135775/1‑73 (MQ=255)
aGTCGCGCAGGAAGTT‑CAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCtggtg < 1:135775/73‑1 (MQ=255)
gTCGCGCAGGAAGTT‑CAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAGGTAGCCCCATAGATGGGcc < 1:710800/133‑1 (MQ=255)
aaGTT‑CAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAGGTAGCCCCATAGATGGGCCTGCCGCgtggt > 2:539332/1‑134 (MQ=255)
|
GAAGAAGCGTCGGCGGCAATGCGCCCAGCATCCGCCCTCGATGATCCGGCCGGTACGGCAAAGCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTT‑CA‑GGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAGGTAGCCCCATAGATGGGCCTGCCGCGTGGT > AM260479/1841073‑1841323
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CTTCTGGGCCTTGGCGATCTCGAAGAAGCGTCGGCGGCAATGCGCCCAGCATCCGCCCTCGATGATCCGGCCGGTACGGCAAAGCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCA‑GGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAGGTAGCCCCATAGATGGGCCTGCCGCGTGGTGGCG > AM260479/1841052‑1841327
|
CTTCTGGGCCTTGGCGATCTCGAAGAAGCGTCGGCGGCAATGCGCCCAGCATCCGCCCTCGATGATCCGGCCGGTACGGCAAAGCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCA‑GGGC > GWNJ‑0478:712:GW2002102894th:2:1203:5020:31287/1‑150 (MQ=60)
TCTGGGCCTTGGCGATCTCGAAGAAGCGTCGGCGGCAATGCGCCCAGCATCCGCCCTCGATGATCCGGCCGGTACGGCAAAGCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCA‑GGGG < GWNJ‑0478:712:GW2002102894th:2:2107:6304:23765/148‑1 (MQ=60)
GCCTTGGCGATCTCGAAGAAGCGTCGGCGGCAATGCGCCCAGCATCCGCCCTCGATGATCCGGCCGGTACGGCAAAGCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCG < GWNJ‑0478:712:GW2002102894th:2:1108:4582:40331/150‑1 (MQ=60)
TGGCGATCTCGAAGAAGCGTCGGCGGCAATGCGCCCAGCATCCGCCCTCGATGATCCGGCCGGTACGGCAAAGCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGG > GWNJ‑0478:712:GW2002102894th:2:2101:6910:95697/1‑150 (MQ=60)
GCGCCCAGCATCCGCCCTCGATGATCCGGCCGGTACGGCAAAGCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCG > GWNJ‑0478:712:GW2002102894th:2:2101:9191:87533/1‑150 (MQ=60)
CATCCGCCCTCGATGATCCGGCCGGTACGGCAAAGCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGG < GWNJ‑0478:712:GW2002102894th:2:1107:20671:83228/150‑1 (MQ=60)
CCCTCGATGATCCGGCCGGTACGGCAAAGCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCG > GWNJ‑0478:712:GW2002102894th:2:1102:19742:24279/1‑150 (MQ=60)
GATCCGGCCGGTACGGCAAAGCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCctgt > GWNJ‑0478:712:GW2002102894th:2:2202:4502:45710/1‑146 (MQ=60)
gagatgtagctccgtctcgtgggctcggagatgtgtataagagacagGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCC < GWNJ‑0478:712:GW2002102894th:2:1111:10022:2250/103‑1 (MQ=60)
cgtgggctcggagatgtgtataagagacagAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTC < GWNJ‑0478:712:GW2002102894th:2:2104:14246:95521/119‑1 (MQ=60)
GTACGGCAAAGCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCC > GWNJ‑0478:712:GW2002102894th:2:2106:6367:78135/1‑150 (MQ=60)
GCAAAGCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCG > GWNJ‑0478:712:GW2002102894th:2:1203:13493:11762/1‑150 (MQ=60)
GCAAAGCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCG < GWNJ‑0478:712:GW2002102894th:2:1101:11895:21669/150‑1 (MQ=60)
GCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAGGT > GWNJ‑0478:712:GW2002102894th:2:2109:8327:62026/1‑149 (MQ=60)
GCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAGGT > GWNJ‑0478:712:GW2002102894th:2:2210:20985:19552/1‑149 (MQ=60)
GTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAGGTAGCC > GWNJ‑0478:712:GW2002102894th:2:1210:9126:91131/1‑150 (MQ=60)
GCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAGGTAGCCCCATAGA < GWNJ‑0478:712:GW2002102894th:2:1111:7860:57394/150‑1 (MQ=60)
CGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAGGTAGCCCCATAGATG < GWNJ‑0478:712:GW2002102894th:2:1210:9112:66567/150‑1 (MQ=60)
CGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAGGTAGCCCCATAGATG < GWNJ‑0478:712:GW2002102894th:2:2107:3260:31194/150‑1 (MQ=60)
AGTCGCGCAGGAAGTTCAGGGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAGGTAGCCCCATAGATGGGCCTGCCGCGTGGTGGCG < GWNJ‑0478:712:GW2002102894th:2:2112:3539:20414/149‑1 (MQ=60)
|
CTTCTGGGCCTTGGCGATCTCGAAGAAGCGTCGGCGGCAATGCGCCCAGCATCCGCCCTCGATGATCCGGCCGGTACGGCAAAGCGCATCGTGGCCGCTGTAGGCAGCCGCCTGCAGGTAGCCATGATAGTCGCGCAGGAAGTTCA‑GGGATGGGCGCCGGCGCGGGATTCCGCGAACTCGAACAGCACCGCTGGCTGGTGGTCGACCCATAATCCGTTCTCCTGCCGCAGCCCGGCCACGAGGTAGCCCCATAGATGGGCCTGCCGCGTGGTGGCG > AM260479/1841052‑1841327
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
