Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
154 |
26.6 |
2069536 |
90.2% |
1866721 |
108.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
2,274,430 |
C→G |
G565G (GGG→GGC) |
h16_A2092 ← |
Lysophospholipase, contains weak SAM‑dependent methyltransferase domain |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 2,274,430 | 0 | C | G | 100.0%
| 42.1
/ NA
| 12 | G565G (GGG→GGC) | h16_A2092 | Lysophospholipase, contains weak SAM‑dependent methyltransferase domain |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (7/5); total (7/5) |
TGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAACCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGCTGG > AM260479/2274352‑2274553
|
tGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCGCCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCAcc > 2:743502/1‑133 (MQ=255)
gccTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGcc > 1:267498/1‑74 (MQ=255)
gccTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGcc < 2:267498/74‑1 (MQ=255)
gccTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGcggcgccggcg > 1:240175/1‑134 (MQ=255)
tGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCgg > 2:113753/1‑134 (MQ=255)
aCGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAgcg < 2:251952/133‑1 (MQ=255)
tGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAgcgcgcgc < 2:240175/134‑1 (MQ=255)
tCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCTCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAAtt > 2:940337/1‑114 (MQ=255)
tCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAAtt < 1:940337/114‑1 (MQ=255)
tCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGc < 1:113753/134‑1 (MQ=255)
ccTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGCTgg > 2:247948/1‑134 (MQ=255)
ccTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGAGCGCGATGAATTCCAGCTGCGGATGCCAGGGCTgg > 2:957170/1‑134 (MQ=255)
|
TGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAACCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGCTGG > AM260479/2274352‑2274553
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
TGCGAATGCGCCCCGGCGCCAACCTCCGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAACCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGCTGGCCGGTGTAGACCAGATAGCCGCCGG > AM260479/2274315‑2274578
|
cagactctttataggttttcttatttgttatttttttttttttcaagcagaagacggcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacagGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCC < GWNJ‑0478:712:GW2002102894th:2:2103:19885:56773/40‑1 (MQ=60)
GTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCctgtctcttatacacatctccgagcccacgagacggagctacatct > GWNJ‑0478:712:GW2002102894th:2:1201:13802:86926/1‑104 (MQ=60)
GTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGC > GWNJ‑0478:712:GW2002102894th:2:1116:8613:6787/1‑150 (MQ=60)
agacaGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCC < GWNJ‑0478:712:GW2002102894th:2:2210:10974:75472/145‑1 (MQ=60)
ataagagacagTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGCTGGCCGGTGT < GWNJ‑0478:712:GW2002102894th:2:1111:6922:66235/138‑1 (MQ=60)
CTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGCTGGCCGGTGT > GWNJ‑0478:712:GW2002102894th:2:2109:4411:55562/1‑149 (MQ=60)
GTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGCTGGCCGGTGTAGACC < GWNJ‑0478:712:GW2002102894th:2:1108:20541:64678/150‑1 (MQ=60)
ACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCctgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtcttctgcttgaaaaaaaaaacagaaaagtgcggaactgacccatatacacatatacg > GWNJ‑0478:712:GW2002102894th:2:1104:18200:94382/1‑36 (MQ=60)
GCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGctgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtcttctgcttgaaa > GWNJ‑0478:712:GW2002102894th:2:2114:8003:57515/1‑80 (MQ=60)
|
TGCGAATGCGCCCCGGCGCCAACCTCCGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAACCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGCTGGCCGGTGTAGACCAGATAGCCGCCGG > AM260479/2274315‑2274578
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
