Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
149 |
26.7 |
2337422 |
87.5% |
2045244 |
105.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
2,274,430 |
C→G |
G565G (GGG→GGC) |
h16_A2092 ← |
Lysophospholipase, contains weak SAM‑dependent methyltransferase domain |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 2,274,430 | 0 | C | G | 100.0%
| 53.7
/ NA
| 15 | G565G (GGG→GGC) | h16_A2092 | Lysophospholipase, contains weak SAM‑dependent methyltransferase domain |
| Reads supporting (aligned to +/- strand): ref base C (0/0); new base G (6/9); total (6/9) |
AGGCAGCTGCGAATGCGCCCCGGCGCCAACCTCCGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAACCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGC > AM260479/2274308‑2274550
|
aggcagCTGCGAATGCGCCCCGGCGCCAACCTCCGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCg > 2:342025/1‑134 (MQ=255)
tGCGCCCCGGCGCCAACCTCCGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTcc < 2:592667/134‑1 (MQ=255)
ccAACCTCCGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGc > 2:562148/1‑134 (MQ=255)
ggCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAgcggcggcg > 1:812366/1‑133 (MQ=255)
aTGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAggcg < 2:812366/134‑1 (MQ=255)
gccgcgccTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGcggcgc < 1:406794/134‑1 (MQ=255)
cgcgccTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGcggcgccg < 2:973275/134‑1 (MQ=255)
gACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAgcg > 2:1083624/1‑134 (MQ=255)
cGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAgcgcg < 1:562148/134‑1 (MQ=255)
tGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAgcgcgcgc < 1:342025/134‑1 (MQ=255)
gCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATg > 2:1132896/1‑133 (MQ=255)
aTACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCg > 2:838405/1‑133 (MQ=255)
ggTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAg < 1:1132896/133‑1 (MQ=255)
tCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGc < 1:838405/134‑1 (MQ=255)
tCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGc < 2:702931/134‑1 (MQ=255)
|
AGGCAGCTGCGAATGCGCCCCGGCGCCAACCTCCGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAACCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGC > AM260479/2274308‑2274550
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CCGGTTGCCTGCAGGCAGCTGCGAATGCGCCCCGGCGCCAACCTCCGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAACCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGCTGGCCGGTGTAGACC > AM260479/2274296‑2274565
|
CCGGTTGCCTGCAGGCAGCTGCGAATGCGCCCCGGCGCCAACCTCCGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACC < GWNJ‑0478:712:GW2002102894th:2:1111:3737:43390/150‑1 (MQ=60)
tccctttgtgtttttttttttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagCGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACC < GWNJ‑0478:712:GW2002102894th:2:2212:3634:25964/62‑1 (MQ=60)
GCGCCAACCTCCGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGC > GWNJ‑0478:712:GW2002102894th:2:2115:10125:34245/1‑149 (MQ=60)
CGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGG < GWNJ‑0478:712:GW2002102894th:2:1208:10176:56198/150‑1 (MQ=60)
ACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCC < GWNJ‑0478:712:GW2002102894th:2:1216:8701:40970/150‑1 (MQ=60)
GTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGC < GWNJ‑0478:712:GW2002102894th:2:1205:17592:69559/150‑1 (MQ=60)
GGGTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGCTGGCCGGTGTAG < GWNJ‑0478:712:GW2002102894th:2:2215:11826:45011/149‑1 (MQ=60)
GTCACCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGCTGGCCGGTGTAGACC < GWNJ‑0478:712:GW2002102894th:2:2114:4316:54272/150‑1 (MQ=60)
CCTTGTCAAAGCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGCTGGCCGGTGTAGACC > GWNJ‑0478:712:GW2002102894th:2:2203:3348:61068/1‑146 (MQ=60)
|
CCGGTTGCCTGCAGGCAGCTGCGAATGCGCCCCGGCGCCAACCTCCGTCGGCACCGTGCTCATGCGCCGCGCCTGCGCGCCAGGCTGACGGTGAAGATGCCCCACGGGTCGATACGCTGGGTCACCTTGTCAAACCCAGCGGCCCGCACCAGTTCGTCCATCTCGGCCTGCGAGCGGCGGCGCATCACCCAGGCGGCGCCGGCGCGGTGGCTGGTGAGCGCGCGCGCGATGAATTCCAGCTGCGGATGCCAGGGCTGGCCGGTGTAGACC > AM260479/2274296‑2274565
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
