Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
154 |
26.6 |
2069536 |
90.2% |
1866721 |
108.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260480 |
919,006 |
T→G |
C159G (TGC→GGC) |
h16_B0812 → |
hydrolase or acyltransferase (alpha/beta hydrolase superfamily) |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260480 | 919,006 | 0 | T | G | 100.0%
| 62.6
/ NA
| 17 | C159G (TGC→GGC) | h16_B0812 | hydrolase or acyltransferase (alpha/beta hydrolase superfamily) |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (10/7); total (10/7) |
CTGGCCAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGTGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCGTGGTGCGCAGCTTTGAGGATTTCCATCGC > AM260480/918901‑919134
|
ctggcCAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCtt < 2:15725/134‑1 (MQ=255)
aCTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGa < 1:432837/134‑1 (MQ=255)
ggTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCtt > 1:953762/1‑117 (MQ=255)
ggTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCtt < 2:953762/117‑1 (MQ=255)
ggTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGAcc > 1:229015/1‑133 (MQ=255)
aCCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGag > 2:171523/1‑134 (MQ=255)
aCCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGag > 2:263570/1‑134 (MQ=255)
cgcgcgTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCt < 2:229015/134‑1 (MQ=255)
cGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGcc > 1:779285/1‑134 (MQ=255)
tCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATg < 1:758486/91‑1 (MQ=255)
tCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATg > 2:758486/1‑91 (MQ=255)
tCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCGTGGTGCGCAGCTTTGAg > 1:612430/1‑134 (MQ=255)
gCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCAt > 1:663771/1‑95 (MQ=255)
gCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCAt < 2:663771/95‑1 (MQ=255)
gcCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCGTGGTGCGCAGCTTTGAGGATTTCCATcg < 1:449470/133‑1 (MQ=255)
gcCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCGTGGTGCGCAGCTTTGAGGATTTCCATcg > 2:449470/1‑133 (MQ=255)
ccAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCGTGGTGCGCAGCTTTGAGGATTTCCATcgc > 2:239313/1‑133 (MQ=255)
|
CTGGCCAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGTGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCGTGGTGCGCAGCTTTGAGGATTTCCATCGC > AM260480/918901‑919134
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GCGCGCGCATCGGCGTGCGCGCCGCGGCGGGCCGGCCTGGCGGCCTGGCCAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGTGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCGTGGTGCGCAGCTTTGAGGATTTCCATCGCGACGACATCCATGC > AM260480/918857‑919148
|
GCGCGCGCATCGGCGTGCGCGCCGCGGCGGGCCGGCCTGGCGGCCTGGCCAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGG > GWNJ‑0478:712:GW2002102894th:2:2202:1969:48110/1‑150 (MQ=60)
CATCGGCGTGCGCGCCGCGGCGGGCCGGCCTGGCGGCCTGGCCAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGA < GWNJ‑0478:712:GW2002102894th:2:2114:14118:46051/150‑1 (MQ=60)
GCGTGCGCGCCGCGGCGGGCCGGCCTGGCGGCCTGGCCAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGG < GWNJ‑0478:712:GW2002102894th:2:1202:15912:87793/150‑1 (MQ=60)
tttttttttttttcaagcagaagacggcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacaGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAA < GWNJ‑0478:712:GW2002102894th:2:1113:8930:55987/71‑1 (MQ=60)
agacggcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacaGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGG < GWNJ‑0478:712:GW2002102894th:2:2203:5230:89667/93‑1 (MQ=60)
GGCCAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCtg > GWNJ‑0478:712:GW2002102894th:2:2212:8824:54085/1‑148 (MQ=60)
GGCCAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCG > GWNJ‑0478:712:GW2002102894th:2:1114:2566:43443/1‑149 (MQ=60)
GACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGC < GWNJ‑0478:712:GW2002102894th:2:1213:10355:30329/150‑1 (MQ=60)
gtgggctcggagatgtgtataagagacagGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAA < GWNJ‑0478:712:GW2002102894th:2:2116:18347:15656/121‑1 (MQ=60)
ATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCG > GWNJ‑0478:712:GW2002102894th:2:2201:9991:44389/1‑150 (MQ=60)
GGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCGTGGTGCGCAGCTTTGAGG > GWNJ‑0478:712:GW2002102894th:2:2106:20044:51002/1‑150 (MQ=60)
ATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACctgtctcttatacacatctccgagc > GWNJ‑0478:712:GW2002102894th:2:2111:17047:68031/1‑125 (MQ=60)
CGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCGTGGTGCGCAGCTTTGAGGATTTCCATCGCGACGAC > GWNJ‑0478:712:GW2002102894th:2:1212:12721:56470/1‑150 (MQ=60)
GCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCGTGGTGCGCAGCTTTGAGGATTTCCATCGCGACGACATCCATGC < GWNJ‑0478:712:GW2002102894th:2:1212:13151:23205/150‑1 (MQ=60)
|
GCGCGCGCATCGGCGTGCGCGCCGCGGCGGGCCGGCCTGGCGGCCTGGCCAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGTGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCGTGGTGCGCAGCTTTGAGGATTTCCATCGCGACGACATCCATGC > AM260480/918857‑919148
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
