Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
149 |
26.7 |
2337422 |
87.5% |
2045244 |
105.0 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260480 |
919,006 |
T→G |
C159G (TGC→GGC) |
h16_B0812 → |
hydrolase or acyltransferase (alpha/beta hydrolase superfamily) |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260480 | 919,006 | 0 | T | G | 100.0%
| 99.8
/ NA
| 28 | C159G (TGC→GGC) | h16_B0812 | hydrolase or acyltransferase (alpha/beta hydrolase superfamily) |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (13/15); total (13/15) |
GCCGCGGCGGGCCGGCCTGGCGGCCTGGCCAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGTGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCGTGGTGCGCAGCTTTGAGGATTTCCATC > AM260480/918877‑919132
|
gccgcGGCGGGCCGGCCTGGCGGCCTGGCCAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCAc > 1:192523/1‑134 (MQ=255)
cctggcggcctggcCAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCAt < 2:192523/134‑1 (MQ=255)
gcggcctggcCAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATgcgc < 2:427191/134‑1 (MQ=255)
ctggcCAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCtt < 1:1139515/134‑1 (MQ=255)
cAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGcc > 1:662774/1‑134 (MQ=255)
aCCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGag > 1:598617/1‑134 (MQ=255)
ccgccgGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGagca < 1:436056/134‑1 (MQ=255)
gccgGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGagcagc > 1:350677/1‑134 (MQ=255)
cccGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCGTGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTgcgcgc < 2:864058/134‑1 (MQ=255)
cccGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTgcgcgc < 2:1040484/134‑1 (MQ=255)
ccGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGcc < 1:37056/134‑1 (MQ=255)
cTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTg > 1:940816/1‑133 (MQ=255)
gcAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGctgcg > 1:10607/1‑102 (MQ=255)
gcAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGctgcg < 2:10607/102‑1 (MQ=255)
aCTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTgcgcgc > 1:42285/1‑108 (MQ=255)
aCTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTgcgcgc < 2:42285/108‑1 (MQ=255)
ttCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACcg > 1:483935/1‑68 (MQ=255)
ttCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACcg < 2:483935/68‑1 (MQ=255)
tCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCAt > 1:764292/1‑98 (MQ=255)
tCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCAt < 2:764292/98‑1 (MQ=255)
ccGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATAcc < 1:24949/100‑1 (MQ=255)
ccGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATAcc > 2:24949/1‑100 (MQ=255)
gCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCGTGGTGCGCAGCTTTGAGGAt > 1:1051505/1‑134 (MQ=255)
cTGTCGCGCCATGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGctgcgcctg < 2:92047/75‑1 (MQ=17)
cTGTCGCGCCATGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGctgcgcctg > 1:92047/1‑75 (MQ=17)
cgcgCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTc > 2:552113/1‑38 (MQ=14)
cgcgCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTc < 1:552113/38‑1 (MQ=14)
gcgcCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCGTGGTGCGCAGCTTTGAGGATTTCCATc < 2:662774/134‑1 (MQ=255)
|
GCCGCGGCGGGCCGGCCTGGCGGCCTGGCCAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGTGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCGTGGTGCGCAGCTTTGAGGATTTCCATC > AM260480/918877‑919132
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GCGCATCGGCGTGCGCGCCGCGGCGGGCCGGCCTGGCGGCCTGGCCAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGTGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCGTGGTGCGCAGCTTTGAGGATTTC > AM260480/918861‑919128
|
catacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacaGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCAC < GWNJ‑0478:712:GW2002102894th:2:2206:13343:41864/99‑1 (MQ=60)
CGCATCGGCGTGCGCGCCGCGGCGGGCCGGCCTGGCGGCCTGGCCAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACC > GWNJ‑0478:712:GW2002102894th:2:1113:9108:39455/1‑150 (MQ=60)
GTGCGCGCCGCGGCGGGCCGGCCTGGCGGCCTGGCCAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAA < GWNJ‑0478:712:GW2002102894th:2:1201:13045:91354/150‑1 (MQ=60)
GCGCGCCGCGGCGGGCCGGCCTGGCGGCCTGGCCAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGG < GWNJ‑0478:712:GW2002102894th:2:2214:8513:61736/150‑1 (MQ=60)
GCCTGGCGGCCTGGCCAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCC > GWNJ‑0478:712:GW2002102894th:2:2107:11415:42455/1‑150 (MQ=60)
CCTGGCCAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGAC < GWNJ‑0478:712:GW2002102894th:2:2215:1659:74228/150‑1 (MQ=60)
atttttttttttttttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacaGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACC < GWNJ‑0478:712:GW2002102894th:2:1214:8449:94277/69‑1 (MQ=60)
GACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGC > GWNJ‑0478:712:GW2002102894th:2:2103:11081:53379/1‑150 (MQ=60)
GGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCT > GWNJ‑0478:712:GW2002102894th:2:1204:11517:23642/1‑150 (MQ=60)
CCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGC < GWNJ‑0478:712:GW2002102894th:2:1211:8979:21499/150‑1 (MQ=60)
CCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACC < GWNJ‑0478:712:GW2002102894th:2:1105:2608:37495/150‑1 (MQ=60)
GGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGC > GWNJ‑0478:712:GW2002102894th:2:2205:4095:41445/1‑149 (MQ=60)
CCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGctgtctcttatacacatc > GWNJ‑0478:712:GW2002102894th:2:1101:11218:70285/1‑132 (MQ=60)
CGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGtctcttat > GWNJ‑0478:712:GW2002102894th:2:1105:4402:69477/1‑141 (MQ=60)
gagatgtgtataagagacaGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCC < GWNJ‑0478:712:GW2002102894th:2:1103:13222:61255/131‑1 (MQ=60)
GCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatg > GWNJ‑0478:712:GW2002102894th:2:1213:5708:67877/1‑98 (MQ=60)
GTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATctgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaactaatacccaaggatgcgct > GWNJ‑0478:712:GW2002102894th:2:1113:11207:2322/1‑54 (MQ=60)
GGTATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACctgtctcttatacacatctccg > GWNJ‑0478:712:GW2002102894th:2:2110:3135:96371/1‑128 (MQ=60)
ATGTGGATTCCATCCGCCTGTCGCGCCAGGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCGTGGTGCGCAGCTTTGAGGATT > GWNJ‑0478:712:GW2002102894th:2:2210:17023:53863/1‑150 (MQ=60)
GTGGATTCCATCCGCCTGTCGCGCCATGGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCtgtctcttatacacatctccgagcccacgagacactcgctaatc > GWNJ‑0478:712:GW2002102894th:2:1107:7840:77978/1‑106 (MQ=60)
|
GCGCATCGGCGTGCGCGCCGCGGCGGGCCGGCCTGGCGGCCTGGCCAGACTGGTGCTGGTCGACCCGCCGGTGTCGGGGCCCGGGCGCCGCGCGTATCCTGCGCAACTGCCGTGGTATGTGGATTCCATCCGCCTGTCGCGCCAGTGCACCGACGTGGAAGGCATGCGCCGCTTCTGCCCGACCTGGACCGACGAGCAGCTGCGCCTGCGCGCCGAATGGCTGCATACCTGCAACGAAGACGCCGTGGTGCGCAGCTTTGAGGATTTC > AM260480/918861‑919128
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
