Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
154 |
26.6 |
2069536 |
90.2% |
1866721 |
108.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260480 |
1,326,761 |
+G |
intergenic (+62/‑108) |
h16_B1172 → / → h16_B1173 |
Amidase/MFS transporter |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260480 | 1,326,760 | 1 | . | G | 100.0%
| 125.9
/ NA
| 33 | intergenic (+61/‑109) | h16_B1172/h16_B1173 | Amidase/MFS transporter |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (16/17); total (16/17) |
CCTATGCGGCCGAGCAGGCCTTGCGCGCGCGCCGGCCGCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCC‑GACAC‑GCGGCGACAA‑GTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCTCGACCGGGCCATGGATGGCATCGGGGTGAC > AM260480/1326635‑1326888
|
ccTATGCGGCCGAGCAGGCCTTGCGCGCGCGCCGGCCGCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAA‑ggtcgc > 2:691145/1‑129 (MQ=255)
tGCGGCCGAGCAGGCCTTGCGCGCGCGCCGGCCGCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGa > 1:871185/1‑133 (MQ=255)
gCAGGCCTTGCGCGCGCGCCGGCCGCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGa > 2:574543/1‑134 (MQ=255)
gCAGGCCTTGCGCGCGCGCCGGCCGCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGa > 2:1033381/1‑134 (MQ=255)
gccgcATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGAc > 2:438678/1‑133 (MQ=255)
gccgcATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACa < 1:900051/134‑1 (MQ=255)
cgcATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACaaa < 1:438678/134‑1 (MQ=255)
cATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGa < 1:574543/134‑1 (MQ=255)
tCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACgaggagga > 2:922570/1‑123 (MQ=255)
tCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACgaggagga < 1:922570/123‑1 (MQ=255)
aCGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGgcg < 1:1033381/134‑1 (MQ=255)
cGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGAc > 2:46055/1‑100 (MQ=255)
cGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGAc < 1:46055/100‑1 (MQ=255)
cgcgcCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGc < 1:897674/134‑1 (MQ=255)
cGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGAt > 2:726852/1‑103 (MQ=255)
cGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGAt < 1:726852/103‑1 (MQ=255)
gacgTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACga < 1:790393/82‑1 (MQ=255)
gacgTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACga > 2:790393/1‑82 (MQ=255)
ctctACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCt < 2:11650/133‑1 (MQ=255)
ctctACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCt < 1:691145/133‑1 (MQ=255)
ctctACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCt < 2:907784/133‑1 (MQ=255)
cgcccgccgGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCt < 1:997136/124‑1 (MQ=255)
cgcccgccgGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCt > 2:997136/1‑124 (MQ=255)
gccggACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCAcag > 2:143012/5‑108 (MQ=255)
gccggACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCAcag < 1:143012/104‑1 (MQ=255)
gccggACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAgcagca < 2:744254/109‑1 (MQ=255)
gccggACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAgcagca > 1:744254/5‑113 (MQ=255)
ccggACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCTCGACCGGGCCatggat > 2:505332/4‑134 (MQ=255)
ggACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCTCGACCGGGCCatggatgg > 2:440770/2‑134 (MQ=255)
ggACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCTCGACCGGGCCatggatgg < 1:505332/133‑1 (MQ=255)
ggACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCTCGACCGGGCCa > 1:936330/2‑127 (MQ=255)
ggACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCTCGACCGGGCCa < 2:936330/126‑1 (MQ=255)
cgACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCTCGACCGGGCCATGGATGGCATCGGGGTGa > 1:782392/1‑134 (MQ=255)
gACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCTCGACCGGGCCATGGATGGCATCGGGGTGAc > 1:388996/1‑134 (MQ=255)
|
CCTATGCGGCCGAGCAGGCCTTGCGCGCGCGCCGGCCGCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCC‑GACAC‑GCGGCGACAA‑GTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCTCGACCGGGCCATGGATGGCATCGGGGTGAC > AM260480/1326635‑1326888
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GATACGGCTGGCCTATGCGGCCGAGCAGGCCTTGCGCGCGCGCCGGCCGCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCC‑GACAC‑GCGGCGACAA‑GTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCTCGACCGGGCCATGGATGGCATCGGGGTGACGGCATCGCACAAGAAGATCATC > AM260480/1326624‑1326910
|
GATACGGCTGGCCTATGCGGCCGAGCAGGCCTTGCGCGCGCGCCGGCCGCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGAC > GWNJ‑0478:712:GW2002102894th:2:2206:11047:53201/1‑149 (MQ=60)
GCTGGCCTATGCGGCCGAGCAGGCCTTGCGCGCGCGCCGGCCGCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACtgtctct > GWNJ‑0478:712:GW2002102894th:2:1115:11419:29244/1‑143 (MQ=60)
gagatgtgtataagagacagGGCCGCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGG < GWNJ‑0478:712:GW2002102894th:2:1102:7578:17339/130‑1 (MQ=60)
ccgtctcgtgggctcggagatgtgtataagagacagGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGAT < GWNJ‑0478:712:GW2002102894th:2:2203:16739:22050/112‑1 (MQ=60)
CGGCCGCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGC < GWNJ‑0478:712:GW2002102894th:2:2211:12199:71618/150‑1 (MQ=60)
CGCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAA < GWNJ‑0478:712:GW2002102894th:2:2208:15279:34307/150‑1 (MQ=60)
CCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAAC < GWNJ‑0478:712:GW2002102894th:2:1213:7459:61823/150‑1 (MQ=60)
GCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAG < GWNJ‑0478:712:GW2002102894th:2:2105:9939:97892/150‑1 (MQ=60)
atgtgtataagagacagGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGT < GWNJ‑0478:712:GW2002102894th:2:2115:2220:38336/133‑1 (MQ=60)
GACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTC < GWNJ‑0478:712:GW2002102894th:2:2216:16868:93383/150‑1 (MQ=60)
GCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGC < GWNJ‑0478:712:GW2002102894th:2:2208:14072:19079/150‑1 (MQ=60)
ataagagacagGCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCTCGAC < GWNJ‑0478:712:GW2002102894th:2:1111:6656:44080/138‑1 (MQ=60)
GCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCTCGACCGGGCCtgtctc > GWNJ‑0478:712:GW2002102894th:2:2114:4477:33455/1‑144 (MQ=60)
GCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCTCGACCGGGCCATGGA < GWNJ‑0478:712:GW2002102894th:2:2113:4001:29791/149‑1 (MQ=60)
GCTCTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCTCGACCGGGCCATGGA < GWNJ‑0478:712:GW2002102894th:2:2214:10380:95397/149‑1 (MQ=60)
CTACAAACGCCCGCCGGACACGGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCTCGACCGGGCCATGGATGGC > GWNJ‑0478:712:GW2002102894th:2:2210:13636:51577/1‑150 (MQ=60)
cgccggacacgGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCTCGACCGGGCCATGGATGGCATCGGGGTGAC > GWNJ‑0478:712:GW2002102894th:2:2201:14988:67067/12‑150 (MQ=60)
gccggacacgGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCTCGACCGGGCCATGGATGGCATCGGGGTGACG > GWNJ‑0478:712:GW2002102894th:2:1209:14992:79264/11‑150 (MQ=60)
cggacacgGCGGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCTCGACCGGGCCATGGATGGCATCGGGGTGACGGC < GWNJ‑0478:712:GW2002102894th:2:1216:16588:22518/142‑1 (MQ=60)
GGCGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCTCGACCGGGCCATGGATGGCATCGGGGTGACGGCATCGCACA > GWNJ‑0478:712:GW2002102894th:2:1214:20317:71107/1‑148 (MQ=60)
CGACAAGGTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCtgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtcttctgcttgaaaaaaaaaa > GWNJ‑0478:712:GW2002102894th:2:1201:8719:42574/1‑74 (MQ=60)
GTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCctgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtcttctg > GWNJ‑0478:712:GW2002102894th:2:1206:16990:86146/1‑88 (MQ=60)
|
GATACGGCTGGCCTATGCGGCCGAGCAGGCCTTGCGCGCGCGCCGGCCGCCGCATTCGACGCCGGCGCTGGAATAGGCGCGCGCCGGAACGACGTTTTATCGCTGCTCTACAAACGCCCGCC‑GACAC‑GCGGCGACAA‑GTCGCGGACATAGCGCGACAACCTCGAAACGAGGAGGAGACAAAGATGGGCGCAGAAACAGTAGTTCAATCGCCGAATTCCCCACAGCAGCATGAGCTCGACCGGGCCATGGATGGCATCGGGGTGACGGCATCGCACAAGAAGATCATC > AM260480/1326624‑1326910
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
