Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
154 |
26.6 |
2069536 |
90.2% |
1866721 |
108.9 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260480 |
2,063,918 |
G→A |
P215L (CCG→CTG) |
h16_B1826 ← |
Acyl‑CoA dehydrogenase |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260480 | 2,063,918 | 0 | G | A | 100.0%
| 77.7
/ NA
| 21 | P215L (CCG→CTG) | h16_B1826 | Acyl‑CoA dehydrogenase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); new base A (11/10); total (11/10) |
ATGCAATGCGACAGCCGCGCCGGCGACAAGCGAACCTGCGCATAACGGAAGCCCTCGTCCACCTCGCCCAGCACCTGGTCCTGCGGCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCGGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGGGCAGGTCGACCAGGAACATGGTGGCCTGCGCGCGCTCACCATCGCCGGTGCGCGCCATCAGGATGCCGGCGCTGG > AM260480/2063792‑2064047
|
aTGCAATGCGACAGCCGCGCCGGCGACAAGCGAACCTGCGCATAACGGAAGCCCTCGTCCACCTCGCCCAGCACCTGGTCCTGCGGCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCATgg > 1:564236/1‑133 (MQ=255)
aaTGCGACAGCCGCGCCGGCGACAAGCGAACCTGCGCATAACGGAAGCCCTCGTCCACCTCGCCCAGCACCTGGTCCTGCGGCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCATGGAACTg < 2:425879/134‑1 (MQ=255)
ccGCGCCGGCGACAAGCGAACCTGCGCATAACGGAAGCCCTCGTCCACCTCGCCCAGCACCTGGTCCTGCGGCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCATGGAACTGTCGATGgtgt < 1:395578/134‑1 (MQ=255)
ggCGACAAGCGAACCTGCGCATAACGGAAGCCCTCGTCCACCTCGCCCAGCACCTGGTCCTGCGGCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCATGGAACTg < 1:466526/117‑1 (MQ=255)
ggCGACAAGCGAACCTGCGCATAACGGAAGCCCTCGTCCACCTCGCCCAGCACCTGGTCCTGCGGCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCATGGAACTg > 2:466526/1‑117 (MQ=255)
aaCCTGCGCATAACGGAAGCCCTCGTCCACCTCGCCCAGCACCTGGTCCTGCGGCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGc < 1:801133/134‑1 (MQ=255)
cACCTCGCCCAGCACCTGGTCCTGCGGCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGGGCAGGTCGAcc < 2:260657/133‑1 (MQ=255)
tGGTCCTGCGGCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGGGCAGGt > 1:334788/1‑112 (MQ=255)
tGGTCCTGCGGCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGGGCAGGt < 2:334788/112‑1 (MQ=255)
tGCGGCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGGGCAGGTCGACCAGGAACATGGTGGCCTgcgcgcg > 2:464244/1‑134 (MQ=255)
gCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCATGGAACTGt > 2:916230/1‑54 (MQ=255)
gCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCATGGAACTGt < 1:916230/54‑1 (MQ=255)
gCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGGGCAGGTCGACCAGGAACATGGTGGCCTGCGCGCGCTCa > 1:777787/1‑134 (MQ=255)
cAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGGGCAGGTCGACCAGGAACATGGTGGCCTGCGCGCGCTCACCATCGc > 2:609077/1‑134 (MQ=255)
aGGACCTGCGCATGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGGGCAGGTCGACCAGGAACATGGTGGCCTGCGCGCGCTCACCATCGCCGGTGCGCGCCAt > 2:530475/1‑134 (MQ=255)
aTGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGGGCAGGTCGACCAGGAACATGGTGGCCTGCGCGCGCTCACCATCGCCgg < 1:763872/113‑1 (MQ=255)
aTGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGGGCAGGTCGACCAGGAACATGGTGGCCTGCGCGCGCTCACCATCGCCgg > 2:763872/1‑113 (MQ=255)
aTGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGGGCAGGTCGACCAGGAACATGGTGGCCTGCGCGCGCTCACCATCGCCGGTgcgcg > 2:134274/1‑119 (MQ=255)
aTGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGGGCAGGTCGACCAGGAACATGGTGGCCTGCGCGCGCTCACCATCGCCGGTgcgcg < 1:134274/119‑1 (MQ=255)
gCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGGGCAGGTCGACCAGGAACATGGTGGCCTGCGCGCGCTCACCATCGCCGGTGCGCGCCATCAGGATGCCGGCGCTgg < 1:530475/134‑1 (MQ=255)
gCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGGGCAGGTCGACCAGGAACATGGTGGCCTGCGCGCGCTCACCATCGCCGGTGCGCGCCATCAGGATGCCGGCGCTgg > 2:573599/1‑134 (MQ=255)
|
ATGCAATGCGACAGCCGCGCCGGCGACAAGCGAACCTGCGCATAACGGAAGCCCTCGTCCACCTCGCCCAGCACCTGGTCCTGCGGCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCGGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGGGCAGGTCGACCAGGAACATGGTGGCCTGCGCGCGCTCACCATCGCCGGTGCGCGCCATCAGGATGCCGGCGCTGG > AM260480/2063792‑2064047
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CCCCCGAACCAGCGCATGCAATGCGACAGCCGCGCCGGCGACAAGCGAACCTGCGCATAACGGAAGCCCTCGTCCACCTCGCCCAGCACCTGGTCCTGCGGCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCGGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGGGCAGGTCGACCAGGAACATGGTGGCCTGCGCGCGCTCACCATCGCCGGTGCGCGCCATCAGGATGCCGGCGCTGGCCCCTTCGGCTCCGG > AM260480/2063777‑2064062
|
CCCCCGAACCAGCGCATGCAATGCGACAGCCGCGCCGGCGACAAGCGAACCTGCGCATAACGGAAGCCCTCGTCCACCTCGCCCAGCACCTGGTCCTGCGGCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCATGGA > GWNJ‑0478:712:GW2002102894th:2:2105:13104:28045/1‑149 (MQ=60)
AACCAGCGCATGCAATGCGACAGCCGCGCCGGCGACAAGCGAACCTGCGCATAACGGAAGCCCTCGTCCACCTCGCCCAGCACCTGGTCCTGCGGCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCATGGAACTGTCG < GWNJ‑0478:712:GW2002102894th:2:1211:10684:42867/150‑1 (MQ=60)
atgcttttgaattgattcttcattgcttgttttttttttcaagcagaagacggcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacagGATCAGGACCTGCGCATGTCCGCCCAGCATGGAACTGTCGATGGT < GWNJ‑0478:712:GW2002102894th:2:1104:13270:41200/45‑1 (MQ=60)
CAGTGCGACAGCCGCGCCGGCGACAAGCGAACCTGCGCATAACGGAAGCCCTCGTCCACCTCGCCCAGCACCTGGTCCTGCGGCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGC < GWNJ‑0478:712:GW2002102894th:2:1215:13623:16929/150‑1 (MQ=60)
caagcagaagacggcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacagGCACCTGGTCCTGCGGCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCA < GWNJ‑0478:712:GW2002102894th:2:2211:12426:34657/84‑1 (MQ=60)
GCCGCGCCGGCGACAAGCGAACCTGCGCATAACGGAAGCCCTCGTCCACCTCGCCCAGCACCTGGTCCTGCGGCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGA < GWNJ‑0478:712:GW2002102894th:2:1211:17052:17214/150‑1 (MQ=60)
GAACCTGCGCATAACGGAAGCCCTCGTCCACCTCGCCCAGCACCTGGTCCTGCGGCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGG < GWNJ‑0478:712:GW2002102894th:2:2203:20378:94418/150‑1 (MQ=60)
ACCTCGCCCAGCACCTGGTCCTGCGGCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGGGCAGGTCGACCAGGAACATGGctgtctct > GWNJ‑0478:712:GW2002102894th:2:1207:18547:44580/1‑142 (MQ=60)
GCACCTGGTCCTGCGGCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGGGCAGGTCGACCAGGAACATGGTGGCCTGCGCGCGCTCAC > GWNJ‑0478:712:GW2002102894th:2:2201:19312:33614/1‑150 (MQ=60)
CGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCA < GWNJ‑0478:712:GW2002102894th:2:1114:20747:87372/66‑1 (MQ=60)
gagacagGATCAGGACCTGCGCATGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGGGCAGGTCGACCAGGAACATGGTGGCCTGCGCGCGCTCACCATCGCCGGTGCGCGCCATCAGGA < GWNJ‑0478:712:GW2002102894th:2:2116:7556:46602/143‑1 (MQ=60)
nnnnnnnnnnnnGACCTGCGCATGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGGGCAGGTCGACCAGGAACATGGTGGCCTGCGCGCGCTCACCATCGCCGGTGCGCGCCATCAGGA < GWNJ‑0478:712:GW2002102894th:2:1214:10067:101325/137‑1 (MQ=60)
GGATCAGGACCTGCGCATGTCCGCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGGGCAGGTCGACCAGGAACATGGTGGCCTGCGCGCGCTCACCATCGCCGGTGCGCGCCATCAGGATGCCGG < GWNJ‑0478:712:GW2002102894th:2:1109:15913:91285/150‑1 (MQ=60)
CGCCCAGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGGGCAGGTCGACCAGGAACATGGTGGCCTGCGCGCGCTCACCATCGCCGGTGCGCGCCATCAGGATGCCGGCGCTGGCCCCTTCGGCTCCGG < GWNJ‑0478:712:GW2002102894th:2:2101:9042:84896/150‑1 (MQ=60)
|
CCCCCGAACCAGCGCATGCAATGCGACAGCCGCGCCGGCGACAAGCGAACCTGCGCATAACGGAAGCCCTCGTCCACCTCGCCCAGCACCTGGTCCTGCGGCACGCGCAGGCCGTCGATCAGGACCTGCGCATGTCCGCCCGGCATGGAACTGTCGATGGTGTCAAGCAGGCGTTCGATGCGGATCGCCGGATGGGGCAGGTCGACCAGGAACATGGTGGCCTGCGCGCGCTCACCATCGCCGGTGCGCGCCATCAGGATGCCGGCGCTGGCCCCTTCGGCTCCGG > AM260480/2063777‑2064062
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
