Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
150 |
26.0 |
2053212 |
90.0% |
1847890 |
107.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
787,769 |
+T |
intergenic (‑549/+743) |
h16_A0728 ← / ← h16_A0729 |
response regulator containing CheY‑like receiver, AAA‑type ATPase, and DNA‑binding domains/transcriptional regulator, LysR‑family |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 787,768 | 1 | . | T | 100.0%
| 98.0
/ NA
| 26 | intergenic (‑548/+744) | h16_A0728/h16_A0729 | response regulator containing CheY‑like receiver, AAA‑type ATPase, and DNA‑binding domains/transcriptional regulator, LysR‑family |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base T (15/11); total (15/11) |
CCCTGCACCTGGCCAGCCGATGCGGACCAGTTCCTGCCGCGTGGCCCACGCTTACAGCGCCAGTCCGAATCCCGCCCTGGGATGCGGGGTCGTCCTGAAACTGATCTTCGGCGAACCCGTGCCAGTGTG‑TGCCGCTTCGGTTTCAGACGTCCCCCTGTTCCGGGCTTCTGTTCGCGCGGACATATGTGCGACGAACGTGCCAGATATGGCTAATCCTTTGATAACAAAAGGAAAAGGAAA‑TTTCGGCTGGC > AM260479/787640‑787890
|
cccTGCACCTGGCCAGCCGATGCGGACCAGTTCCTGCCGCGTGGCCCACGCTTACAGCGCCAGTCCGAATCCCGCCCTGGGATGCGGGGTCGTCCTGAAACTGATCTTCGGCGAACCCGTGCCAgtgtg‑ttgcc < 1:699295/134‑5 (MQ=255)
cccTGCACCTGGCCAGCCGATGCGGACCAGTTCCTGCCGCGTGGCCCACGCTTACAGCGCCAGTCCGAATCCCGCCCTGGGATGCGGGGTCGTCCTGAAACTGATCTTCGGCGAACCCGTGCCAgtgtg‑ttgcc < 1:974988/134‑5 (MQ=255)
ccAGTTCCTGCCGCGTGGCCCACGCTTACAGCGCCAGTCCGAATCCCGCCCTGGGATGCGGGGTCGTCCTGAAACTGATCTTCGGCGAACCCGTGCCAGTGTGTTGCCGCTTCGGTTTCAGACGTCCCCCTGtt < 2:667873/134‑1 (MQ=255)
tGCCGCGTGGCCCACGCTTACAGCGCCAGTCCGAATCCCGCCCTGGGATGCGGGGTCGTCCTGAAACTGATCTTCGGCGAACCCGTGCCAGTGTGTTGCCGCTTCGGTTTCAGACGTCCCCCTGTTCCGGGCtt > 2:684877/1‑134 (MQ=255)
cgTGGCCCACGCTTACAGCGCCAGTCCGAATCCCGCCCTGGGATGCGGGGTCGTCCTGAAACTGATCTTCGGCGAACCCGTGCCAGTGTGTTGCCGCTTCgg < 1:495048/102‑1 (MQ=255)
cgTGGCCCACGCTTACAGCGCCAGTCCGAATCCCGCCCTGGGATGCGGGGTCGTCCTGAAACTGATCTTCGGCGAACCCGTGCCAGTGTGTTGCCGCTTCgg > 2:495048/1‑102 (MQ=255)
cccACGCTTACAGCGCCAGTCCGAATCCCGCCCTGGGATGCGGGGTCGTCCTGAAACTGATCTTCGGCGAACCCGTGCCAGTGTGTTGCCGCTTCGGTTTCAGACGTCCCCCTGTTCCGGGCTTCTGTTcgcgc > 2:294543/1‑134 (MQ=255)
cccACGCTTACAGCGCCAGTCCGAATCCCGCCCTGGGATGCGGGGTCGTCCTGAAACTGATCTTCGGCGAACCCGTGCCAGTGTGTTGCCGCTTCGGTTTCAGACGTCCCCCTGTTCCGGGCTTCTGTTcgcgc > 2:281364/1‑134 (MQ=255)
gcCAGTCCGAATCCCGCCCTGGGATGCGGGGTCGTCCTGAAACTGATCTTCGGCGAACCCGTGCCAGTGTGTTGCCGCTTCGGTTTCAGACGTCCCCCTGTTCCGGGCTTCTGTTcgcg > 1:265301/1‑119 (MQ=255)
gcCAGTCCGAATCCCGCCCTGGGATGCGGGGTCGTCCTGAAACTGATCTTCGGCGAACCCGTGCCAGTGTGTTGCCGCTTCGGTTTCAGACGTCCCCCTGTTCCGGGCTTCTGTTcgcg < 2:265301/119‑1 (MQ=255)
ccAGTCCGAATCCCGCCCTGGGATGCGGGGTCGTCCTGAAACTGATCTTCGGCGAACCCGTGCCAGTGTGTTGCCGCTTCGGTTTCAGACGTCCCCCTGTTCCGGGCTTCTGTTCGCGCGGACATATGTGcgac < 1:294543/134‑1 (MQ=255)
cGAATCCCGCCCTGGGATGCGGGGTCGTCCTGAAACTGATCTTCGGCGAACCCGTGCCAGTGTGTTGCCGCTTCGGTTTCAGACGTCCCCCTGTTCCGGGCTTCTGTTCGCGCGGACATATGTGCGACGAACGt > 2:659668/1‑134 (MQ=255)
aaTCCCGCCCTGGGATGCGGGGTCGTCCTGAAACTGATCTTCGGCGAACCCGTGCCAGTGTGTTGCCGCTTCGGTTTCAGACGTCCCCCTGTTCCGGGCTTCTGTTCGCGCGGACATATGTGCGACGAACGTGc < 1:684877/134‑1 (MQ=255)
aTCCCGCCCTGGGATGCGGGGTCGTCCTGAAACTGATCTTCGGCGAACCCGTGCCAGTGTGTTGCCGCTTCGGTTTCAGACGTCCCCCTGTTCCGGGCTTCTGTTCGCGCGGACATATGTGCGACGAACGTGcc < 1:281364/134‑1 (MQ=255)
ccGCCCTGGGATGCGGGGTCGTCCTGAAACTGATCTTCGGCGAACCCGTGCCAGTGTGTTGCCGCTTCGGTTTCAGACGTCCCCCTGTTCCGGGCTTCTGTTCGCGCGGACATATGTGCGACGAACGTGc > 2:887646/1‑130 (MQ=255)
gtcgtcCTGAAACTGATCTTCGGCGAACCCGTGCCAGTGTGTTGCCGCTTCgg < 2:62417/53‑1 (MQ=39)
gtcgtcCTGAAACTGATCTTCGGCGAACCCGTGCCAGTGTGTTGCCGCTTCgg > 1:62417/1‑53 (MQ=255)
aTCTTCGGCGAACCCGTGCCAGTGTGTTGCCGCTTCGGTTTCAGACGTCCCCCTGTTCCGGGCTTCTGTTCGCGCGGACATATGTGCGACGAACGTGcc < 1:750296/99‑1 (MQ=255)
aTCTTCGGCGAACCCGTGCCAGTGTGTTGCCGCTTCGGTTTCAGACGTCCCCCTGTTCCGGGCTTCTGTTCGCGCGGACATATGTGCGACGAACGTGcc > 2:750296/1‑99 (MQ=255)
tCGGCGAACCCGTGCCAGTGTGTTGCCGCTTCGGTTTCAGACGTccc > 2:434185/1‑47 (MQ=255)
tCGGCGAACCCGTGCCAGTGTGTTGCCGCTTCGGTTTCAGACGTccc < 1:434185/47‑1 (MQ=255)
tCGGCGAACCCGTGCCAGTGTGTTGCCGCTTCGGTTTCAGACGTCCCCCTGTTCCGGGCTTCTGt > 1:680667/1‑65 (MQ=255)
tCGGCGAACCCGTGCCAGTGTGTTGCCGCTTCGGTTTCAGACGTCCCCCTGTTCCGGGCTTCTGt < 2:680667/65‑1 (MQ=255)
tCGGCGAACCCGTGCCAGTGTGTTGCCGCTTCGGTTTCAGACGTCCCCCTGTTCCGGGCTTCTGTTCGCGCGGACATATGTGCGACGaa < 2:161902/89‑1 (MQ=255)
tCGGCGAACCCGTGCCAGTGTGTTGCCGCTTCGGTTTCAGACGTCCCCCTGTTCCGGGCTTCTGTTCGCGCGGACATATGTGCGACGaa > 1:161902/1‑89 (MQ=255)
tCGGCGAACCCGTGCCAGTGTGTTGCCGCTTCGGTTTCAGACGTCCCCCTGTTCCGGGCTTCTGTTCGCGCGGACATATGTGCGACGAACGTGCCAGATATGGCTAATCCTTTGATAACAAAaggaaaaggaaa > 2:325765/1‑134 (MQ=255)
tCGGCGAACCCGTGCCAGTGTGTTGCCGCTTCGGTTTCAGACGTCCCCCTGTTCCGGGCTTCTGTTCGCGCGGACATATGTGCGACGAACGTGCCAGATATGGCTAATCCTTTGATAACAAAaggaaaaggaaa > 2:359730/1‑134 (MQ=255)
tGCCAGTGTGTTGCCGCTTCGGTTTCAGACGTCCCCCTGTTCCGGGCTTCTGTTCGCGCGGACATATGTGCGACGAACGTGCCAGATATGGCTAATCCTTTGATAACAAAAGGAAAAGGAAATTTTCGGCTGGc > 1:118936/1‑134 (MQ=255)
|
CCCTGCACCTGGCCAGCCGATGCGGACCAGTTCCTGCCGCGTGGCCCACGCTTACAGCGCCAGTCCGAATCCCGCCCTGGGATGCGGGGTCGTCCTGAAACTGATCTTCGGCGAACCCGTGCCAGTGTG‑TGCCGCTTCGGTTTCAGACGTCCCCCTGTTCCGGGCTTCTGTTCGCGCGGACATATGTGCGACGAACGTGCCAGATATGGCTAATCCTTTGATAACAAAAGGAAAAGGAAA‑TTTCGGCTGGC > AM260479/787640‑787890
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
CCCCTGCACCTGGCCAGCCGATGCGGACCAGTTCCTGCCGCGTGGCCCACGCTTACAGCGCCAGTCCGAATCCCGCCCTGGGATGCGGGGTCGTCCTGAAACTGATCTTCGGCGAACCCGTGCCAGTGTG‑TGCCGCTTCGGTTTCAGACGTCCCCCTGTTCCGGGCTTCTGTTCGCGCGGACATATGTGCGACGAACGTGCCAGATATGGCTAATCCTTTGATAACAAAAGGAAAAGGAAA‑TTTCGGCTGGCCTTGGGCAATTGCTGGCGATCAATAA > AM260479/787639‑787916
|
CCCCTGCACCTGGCCAGCCGATGCGGACCAGTTCCTGCCGCGTGGCCCACGCTTACAGCGCCAGTCCGAATCCCGCCCTGGGATGCGGGGTCGTCCTGAAACTGATCTTCGGCGAACCCGTGCCAGTGTGTTGCCGCTTCGGTTTCAGAC < GWNJ‑0478:712:GW2002102894th:2:2113:20441:99303/150‑1 (MQ=60)
CCCCTGCACCTGGCCAGCCGATGCGGACCAGTTCCTGCCGCGTGGCCCACGCTTACAGCGCCAGTCCGAATCCCGCCCTGGGATGCGGGGTCGTCCTGAAACTGATCTTCGGCGAACCCGTGCCAGTGTGTTGCCGCTTCGGTTTCAGAC < GWNJ‑0478:712:GW2002102894th:2:2214:6943:6716/150‑1 (MQ=60)
gatgtgtataagagacaGGACCAGTTCCTGCCGCGTGGCCCACGCTTACAGCGCCAGTCCGAATCCCGCCCTGGGATGCGGGGTCGTCCTGAAACTGATCTTCGGCGAACCCGTGCCAGTGTGTTGCCGCTTCGGTTTCAGACGTCCCCC < GWNJ‑0478:712:GW2002102894th:2:1214:16766:86657/133‑1 (MQ=60)
ttttttcaagcagaagacggcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacaGTCCTGAAACTGATCTTCGGCGAACCCGTGCCAGTGTGTTGCCGCTTCGGTTTCAGACGTCCCCCTGTTCCGGGCTTC < GWNJ‑0478:712:GW2002102894th:2:1213:16523:47924/78‑1 (MQ=60)
GCCCACGCTTACAGCGCCAGTCCGAATCCCGCCCTGGGATGCGGGGTCGTCCTGAAACTGATCTTCGGCGAACCCGTGCCAGTGTGTTGCCGCTTCGGTTTCAGACGTCCCCCTGTTCCGGGCTTCTGTTCGCGCGGACATATGTGCGAC > GWNJ‑0478:712:GW2002102894th:2:1201:9071:89855/1‑150 (MQ=60)
GCCAGTCCGAATCCCGCCCTGGGATGCGGGGTCGTCCTGAAACTGATCTTCGGCGAACCCGTGCCAGTGTGTTGCCGCTTCGGTTTCAGACGTCCCCCTGTTCCGGGCTTCTGTTCGCGCGGACATATGTGCGACGAACGTGCCAGATAT < GWNJ‑0478:712:GW2002102894th:2:1202:6276:91275/150‑1 (MQ=60)
GAATCCCGCCCTGGGATGCGGGGTCGTCCTGAAACTGATCTTCGGCGAACCCGTGCCAGTGTGTTGCCGCTTCGGTTTCAGACGTCCCCCTGTTCCGGGCTTCTGTTCGCGCGGACATATGTGCGACGAACGTGCCAGATATGGCTAATC < GWNJ‑0478:712:GW2002102894th:2:2113:9941:14093/150‑1 (MQ=60)
ggagatgtgtataagagacaGGTCGTCCTGAAACTGATCTTCGGCGAACCCGTGCCAGTGTGTTGCCGCTTCGGTTTCAGACGTCCCCCTGTTCCGGGCTTCTGTTCGCGCGGACATATGTGCGACGAACGTGCCAGATATGGCTAATCC < GWNJ‑0478:712:GW2002102894th:2:2114:16559:100149/130‑1 (MQ=60)
cgagatgtagctccgtctcgtgggctcggagatgtgtataagagacagCCCGTGCCAGTGTGTTGCCGCTTCGGTTTCAGACGTCCCCCTGTTCCGGGCTTCTGTTCGCGCGGACATATGTGCGACGAACGTGCCAGATATGGCTAATCC < GWNJ‑0478:712:GW2002102894th:2:1103:20683:58054/102‑1 (MQ=60)
AATCCCGCCCTGGGATGCGGGGTCGTCCTGAAACTGATCTTCGGCGAACCCGTGCCAGTGTGTTGCCGCTTCGGTTTCAGACGTCCCCCTGTTCCGGGCTTCTGTTCGCGCGGACATATGTGCGACGAACGTGCCAGATATGGCTAATCC < GWNJ‑0478:712:GW2002102894th:2:1202:12983:2812/150‑1 (MQ=60)
GCCCTGGGATGCGGGGTCGTCCTGAAACTGATCTTCGGCGAACCCGTGCCAGTGTGTTGCCGCTTCGGTTTCAGACGTCCCCCctgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtcttctgcttg > GWNJ‑0478:712:GW2002102894th:2:1104:12435:27149/1‑83 (MQ=60)
TCCTGAAACTGATCTTCGGCGAACCCGTGCCAGTGTGTTGCCGCTTCGGTTTCAGACGTCCCCCTGTTCCGGGCTTCTGTTCGCGCGGACATATGctgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccg > GWNJ‑0478:712:GW2002102894th:2:2110:20309:88862/1‑95 (MQ=60)
TCCTGAAACTGATCTTCGGCGAACCCGTGCCAGTGTGTTGCCGCTTCGGTTTCAGACGTCCCCCTGTTCCGGGCTTCTGTTCGCGCGGACATATGTGCGACGAACGTGCCAGATATGGCctgtctcttatacacatctccgagcccacg > GWNJ‑0478:712:GW2002102894th:2:1110:5115:52365/1‑119 (MQ=60)
TCTTCGGCGAACCCGTGCCAGTGTGTTGCCGCTTCGGTTTCAGACGTCCCCCTGTTCCGGGCTTCTGTTCGCGCGGACATATGTGCGACGAACGTGCCAGATATGGCTAATCCTTTGATAACAAAAGGAAAAGGAAATTTTCGGCTGGCC > GWNJ‑0478:712:GW2002102894th:2:1108:11894:90197/1‑150 (MQ=60)
cagGTGCCAGTGTGTTGCCGCTTCGGTTTCAGACGTCCCCCTGTTCCGGGCTTCTGTTCGCGCGGACATATGTGCGACGAACGTGCCAGATATGGCTAATCCTTTGATAACAAAAGGAAAAGGAAATTTTCGGCTGGCCTTGGGCAATTG < GWNJ‑0478:712:GW2002102894th:2:1114:8963:87385/147‑1 (MQ=60)
GTGTGTTGCCGCTTCGGTTTCAGACGTCCCCCTGTTCCGGGCTTCTGTTCGCGCGGACATATGTGCGACGAACGTGCCAGATATGGCctgtctcttatacacatctccgagcccacgagacggagctacaactcgtatgccgtcttctgc > GWNJ‑0478:712:GW2002102894th:2:2216:7455:93677/1‑87 (MQ=60)
TGCCGCTTCGGTTTCAGACGTCCCCCTGTTCCGGGCTTCTGTTCGCGCGGACATATGTGCGACGAACGTGCCAGATATGGCTAATCCTTTGATAACAAAAGGAAAAGGAAATTTTCGGCTGGCCTTGGGCAATTGCTGGCGATCAATAA < GWNJ‑0478:712:GW2002102894th:2:1203:14743:42398/149‑1 (MQ=60)
|
CCCCTGCACCTGGCCAGCCGATGCGGACCAGTTCCTGCCGCGTGGCCCACGCTTACAGCGCCAGTCCGAATCCCGCCCTGGGATGCGGGGTCGTCCTGAAACTGATCTTCGGCGAACCCGTGCCAGTGTG‑TGCCGCTTCGGTTTCAGACGTCCCCCTGTTCCGGGCTTCTGTTCGCGCGGACATATGTGCGACGAACGTGCCAGATATGGCTAATCCTTTGATAACAAAAGGAAAAGGAAA‑TTTCGGCTGGCCTTGGGCAATTGCTGGCGATCAATAA > AM260479/787639‑787916
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
