Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A1 F1 I1 R1
|
150 |
26.0 |
2053212 |
90.0% |
1847890 |
107.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
916,512 |
T→C |
V341A (GTG→GCG) |
h16_A0836 → |
predicted exporter of the RND superfamily |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 916,512 | 0 | T | C | 100.0%
| 81.1
/ NA
| 22 | V341A (GTG→GCG) | h16_A0836 | predicted exporter of the RND superfamily |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (9/13); total (9/13) |
ACACGGTATCCAGCAGATCAACTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGTGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGGCGTGGGCTAC > AM260479/916384‑916591
|
acacGGTATCCAGCAGATCAACTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCt < 2:833630/134‑1 (MQ=255)
tATCCAGCAGATCAACTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTc > 2:73283/1‑130 (MQ=255)
tATCCAGCAGATCAACTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTc < 1:73283/130‑1 (MQ=255)
tATCCAGCAGATCAACTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCa < 2:115376/134‑1 (MQ=255)
gcagATCAACTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTa < 2:611001/134‑1 (MQ=255)
aTCAACTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATtct < 1:888588/134‑1 (MQ=255)
aCTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCatc > 2:12072/1‑134 (MQ=255)
tCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCatcccgat < 2:891374/133‑1 (MQ=255)
ccGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGCCGAGCCTCGTCACGCTTATTCTCATCCCGATCCCGACGATCCGCGAACTGGCGATCACcgcg > 2:671720/1‑134 (MQ=255)
cATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCgag < 1:531365/113‑1 (MQ=255)
cATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCgag > 2:531365/1‑113 (MQ=255)
cATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCgg < 1:266649/131‑1 (MQ=255)
cATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCgg > 2:266649/1‑131 (MQ=255)
cATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCa > 2:12217/1‑134 (MQ=255)
ttGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATc < 1:12217/134‑1 (MQ=255)
ccgccCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCgg < 1:12072/133‑1 (MQ=255)
gCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCgg > 1:810857/1‑93 (MQ=255)
gCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCgg < 2:810857/93‑1 (MQ=255)
ggCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGGCGTGGGCTAc > 2:698106/1‑100 (MQ=255)
ggCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGGCGTGGGCTAc < 1:698106/100‑1 (MQ=255)
gcTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCa > 2:593836/1‑62 (MQ=255)
gcTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCa < 1:593836/62‑1 (MQ=255)
|
ACACGGTATCCAGCAGATCAACTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGTGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGGCGTGGGCTAC > AM260479/916384‑916591
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GTTCGCGATCGGTGTCTCACACGGTATCCAGCAGATCAACTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGTGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGGCGTGGGCTACAAGATCGTCACCAACCTGATGATGTTGCCGGT > AM260479/916366‑916623
|
GTTCGCGATCGGTGTCTCACACGGTATCCAGCAGATCAACTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCG > GWNJ‑0478:712:GW2002102894th:2:2208:5483:14518/1‑148 (MQ=60)
CACACGGTATCCAGCAGATCAACTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTctgt > GWNJ‑0478:712:GW2002102894th:2:1110:11547:50720/1‑146 (MQ=60)
CACGGTATCCAGCAGATCAACTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTC < GWNJ‑0478:712:GW2002102894th:2:1104:11730:99751/150‑1 (MQ=60)
GATCAACTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGAT < GWNJ‑0478:712:GW2002102894th:2:2207:7796:95621/150‑1 (MQ=60)
gagacagGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCG < GWNJ‑0478:712:GW2002102894th:2:2103:4590:22119/143‑1 (MQ=60)
agacggcatacgagatgtagctccgtctcgtgggctcggagatgtgtataagagacagGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGGCG < GWNJ‑0478:712:GW2002102894th:2:2107:3542:49054/92‑1 (MQ=60)
CCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGGCGTGGGCT < GWNJ‑0478:712:GW2002102894th:2:1201:8175:99757/150‑1 (MQ=60)
ATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGGCGTGGGCTACAAG < GWNJ‑0478:712:GW2002102894th:2:1101:14451:87543/150‑1 (MQ=60)
cgagatgtagctccgtctcgtgggctcggagatgtgtataagagacagGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGGCGTGGGCTACAAGAT < GWNJ‑0478:712:GW2002102894th:2:1212:2912:97601/102‑1 (MQ=60)
GCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGGCGTGGGCTACAAGAT < GWNJ‑0478:712:GW2002102894th:2:1101:16353:86712/149‑1 (MQ=60)
gagatgtgtataagagacagGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGGCGTGGGCTACAAGATCGTCACCAAC < GWNJ‑0478:712:GW2002102894th:2:2113:1485:91995/130‑1 (MQ=60)
GCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGGCGTGGGCTACAAGATCtgtctcttatacacatctccgagccc > GWNJ‑0478:712:GW2002102894th:2:2204:10968:3682/1‑124 (MQ=60)
|
GTTCGCGATCGGTGTCTCACACGGTATCCAGCAGATCAACTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGTGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGGCGTGGGCTACAAGATCGTCACCAACCTGATGATGTTGCCGGT > AM260479/916366‑916623
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
