Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
146 |
27.9 |
2327082 |
89.7% |
2087392 |
107.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
916,512 |
T→C |
V341A (GTG→GCG) |
h16_A0836 → |
predicted exporter of the RND superfamily |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 916,512 | 0 | T | C | 100.0%
| 119.7
/ NA
| 33 | V341A (GTG→GCG) | h16_A0836 | predicted exporter of the RND superfamily |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base C (16/17); total (16/17) |
TCCAGCAGATCAACTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGTGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGGCGTGGGCTACAAGATCGTCACCAACCTGATGATGTTGCCGGTG > AM260479/916392‑916624
|
tCCAGCAGATCAACTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACg > 1:899070/1‑134 (MQ=255)
tCCAGCAGATCAACTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACg > 2:393816/1‑134 (MQ=255)
cAACTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCa > 2:507728/1‑134 (MQ=255)
aCTTTATCGTCGGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCatc > 2:249165/1‑134 (MQ=255)
aCTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCt < 2:22056/114‑1 (MQ=255)
aCTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCt > 1:22056/1‑114 (MQ=255)
aCTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCa < 1:295003/120‑1 (MQ=255)
aCTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCa > 2:295003/1‑120 (MQ=255)
aCTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCatc < 2:899070/134‑1 (MQ=255)
aCTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCatc < 1:161274/134‑1 (MQ=255)
aCTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCa < 2:644757/132‑1 (MQ=255)
aCTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCa > 1:644757/1‑132 (MQ=255)
tCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCatcccgatccc > 2:269149/1‑133 (MQ=255)
cgAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCt < 1:435912/101‑1 (MQ=255)
cgAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCt > 2:435912/1‑101 (MQ=255)
cgAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCgatga > 2:963671/1‑134 (MQ=255)
aGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATc > 1:1096519/1‑134 (MQ=255)
gggTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTgg < 2:523726/134‑1 (MQ=255)
ccGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGAt < 1:391987/133‑1 (MQ=255)
cGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACcgcg < 1:494119/133‑1 (MQ=255)
ccATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGc > 1:465578/1‑133 (MQ=255)
cATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCa > 1:471202/1‑134 (MQ=255)
ttGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATc < 1:249165/134‑1 (MQ=255)
ccgccCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTAGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCgg < 2:465578/133‑1 (MQ=255)
gcgcAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGGCGTGGGc > 1:530225/1‑133 (MQ=255)
aCCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGGCGTGGGCTACAAGATCGt < 2:1151699/134‑1 (MQ=255)
aCCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGGCGTGGGCTACAAGATCGt < 1:507728/134‑1 (MQ=255)
aCCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGGCGTGGGCTACAAGATCGt < 2:530225/134‑1 (MQ=255)
gctgATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCt < 1:733880/44‑1 (MQ=255)
gctgATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCt > 2:733880/1‑44 (MQ=255)
ggATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCa > 2:1040626/1‑40 (MQ=255)
ggATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCa < 1:1040626/40‑1 (MQ=25)
gcgcTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGGCGTGGGCTACAAGATCGTCACCAACCTGATGATGTTGCCGGTg < 1:963671/132‑1 (MQ=255)
|
TCCAGCAGATCAACTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGTGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGGCGTGGGCTACAAGATCGTCACCAACCTGATGATGTTGCCGGTG > AM260479/916392‑916624
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
GTGTCTCACACGGTATCCAGCAGATCAACTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGTGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGGCGTGGGCTACAAGATCGTCACCAACCTGATGATGTTGCCGGTGGCGGCGTCGTACTTCCGGTT > AM260479/916377‑916644
|
GTGTCTCACACGGTATCCAGCAGATCAACTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGC > GWNJ‑0478:712:GW2002102894th:2:2203:17895:84513/1‑150 (MQ=60)
agatgtgtataagagacagCAACTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTC < GWNJ‑0478:712:GW2002102894th:2:1209:6157:77266/131‑1 (MQ=60)
tttttttttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacaGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTC < GWNJ‑0478:712:GW2002102894th:2:2111:3696:7038/75‑1 (MQ=60)
GTATCCAGCAGATCAACTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCctgtct > GWNJ‑0478:712:GW2002102894th:2:1103:15984:42213/1‑144 (MQ=60)
GTATCCAGCAGATCAACTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCC < GWNJ‑0478:712:GW2002102894th:2:1201:3006:53658/150‑1 (MQ=60)
GTATCCAGCAGATCAACTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCC > GWNJ‑0478:712:GW2002102894th:2:2105:9497:90109/1‑150 (MQ=60)
tttttttttttcaagcagaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCC < GWNJ‑0478:712:GW2002102894th:2:2211:20217:96342/70‑1 (MQ=60)
AACTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATC < GWNJ‑0478:712:GW2002102894th:2:1111:6455:53407/150‑1 (MQ=60)
ACTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCC > GWNJ‑0478:712:GW2002102894th:2:2213:4137:83529/1‑150 (MQ=60)
AGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCC > GWNJ‑0478:712:GW2002102894th:2:1210:14613:30389/1‑149 (MQ=60)
GGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCAT > GWNJ‑0478:712:GW2002102894th:2:1210:9935:59291/1‑150 (MQ=60)
ACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGG < GWNJ‑0478:712:GW2002102894th:2:1206:16114:33814/149‑1 (MQ=60)
CCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGGCGTGGGC < GWNJ‑0478:712:GW2002102894th:2:1213:3055:73340/149‑1 (MQ=60)
CGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGGCGTGGGCT > GWNJ‑0478:712:GW2002102894th:2:1215:2289:49207/1‑149 (MQ=60)
ATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGGCGTGGGCTACAAG < GWNJ‑0478:712:GW2002102894th:2:1114:10175:6038/150‑1 (MQ=60)
CACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGGCGTGGGCTACAAGATCGTCACCAACCTGATGAT < GWNJ‑0478:712:GW2002102894th:2:1212:20746:40519/150‑1 (MQ=60)
GGCGCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGGCGTGGGCTACAAGATCGTCACCAACCTGATGATGTTGCCGGTGGCGGCGTCGTACTTC < GWNJ‑0478:712:GW2002102894th:2:2209:20828:69828/148‑1 (MQ=60)
GCTGATCACCGCGCTGGCGAGCTTCGTCACGCTTATTCTCATCCCtgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaaaaaaaaacacaacaaatataaattacatacattc > GWNJ‑0478:712:GW2002102894th:2:1102:16918:46468/1‑45 (MQ=60)
|
GTGTCTCACACGGTATCCAGCAGATCAACTTTATCGTCCGCGAGGTCGCCCAGGGTACCGACTCCGCCATTGCCGCCCGGCGCAGCTTCACCGGCCTGCTGATTCCCGGATCGCTGGCGCTGATCACCGCGCTGGTGAGCTTCGTCACGCTTATTCTCATCCCGATCCCGATGATCCGAGAACTGGCGATCACCGCGGCCATCGGCGTGGGCTACAAGATCGTCACCAACCTGATGATGTTGCCGGTGGCGGCGTCGTACTTCCGGTT > AM260479/916377‑916644
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
