Sample Resequencing Stats

Note: The mutation counts shown below represent unfiltered mutation sets.
ALE, Flask, Isolate Predicted Mutations Mean Coverage Total Reads Percent Mapped Mapped Reads Average Read Length
A1 F1 I1 R1 150 26.0 2053212 90.0% 1847890 107.8

Breseq alignment

BRESEQ :: Evidence
Predicted mutation
evidence seq id position mutation annotation gene description
RA AM260480 507,425 +A coding (27/564 nt) h16_B0448 → fragment of ABC‑type transport system,periplasmic component: APC family

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*AM260480507,4231.A97.2% 132.7 / ‑3.3 36coding (25/564 nt)h16_B0448fragment of ABC‑type transport system,periplasmic component: APC family
Reads supporting (aligned to +/- strand):  ref base . (1/0);  new base A (17/18);  total (18/18)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.79e-01

ATACCACGGGCGGCGTTCGCCGCCTGACATGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGGCATTCTTGCCACCCTTTCGGGGCCGTCGGCCGATGTCGGGCGCGACCAATA  >  AM260480/507313‑507550
                                                                                                               |                                                                                                                                
aTACCACGGGCGGCGTTCGCCGCCTGACATGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATcgc                                                                                                           >  2:447937/1‑134 (MQ=255)
aTACCACGGGCGGCGTTCGCCGCCTGACATGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATcg                                                                                                            <  1:373757/133‑1 (MQ=255)
aTACCACGGGCGGCGTTCGCCGCCTGACATGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATcg                                                                                                            >  2:373757/1‑133 (MQ=255)
     aCGGGCGGCGTTCGCCGCCTGACATGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATcgccgccg                                                                                                      >  2:331375/1‑134 (MQ=255)
            gcgTTCGCCGCCTGACATGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCgcgctggc                                                                                               >  1:868645/1‑134 (MQ=255)
               ttCGCCGCCTGACATGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCgcgctggcgct                                                                                            >  2:936718/1‑134 (MQ=255)
                   ccgccTGACATGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTc                                                                                        <  1:936718/134‑1 (MQ=255)
                             tGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGgcgc                                                                              <  1:331375/134‑1 (MQ=255)
                             tGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGgcgc                                                                              <  1:447937/134‑1 (MQ=255)
                                  aGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGcc                                                                         <  1:787590/134‑1 (MQ=255)
                                  aGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGcc                                                                         <  2:868645/134‑1 (MQ=255)
                                                     cGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGgcgc                                                                              >  1:942458/1‑110 (MQ=255)
                                                     cGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGgcgc                                                                              <  2:942458/110‑1 (MQ=255)
                                                     cGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCg                                                      >  2:548153/1‑134 (MQ=255)
                                                     cGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCg                                                      >  1:514583/1‑134 (MQ=255)
                                                                              gACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATcg                                                                                                            >  1:526405/1‑55 (MQ=255)
                                                                              gACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATcg                                                                                                            <  2:526405/55‑1 (MQ=255)
                                                                              gACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAg                                                                       <  1:947052/92‑1 (MQ=255)
                                                                              gACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAg                                                                       >  2:947052/1‑92 (MQ=255)
                                                                              gACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCtga                                                                 >  1:278854/1‑98 (MQ=255)
                                                                              gACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCtga                                                                 <  2:278854/98‑1 (MQ=255)
                                                                              gACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGGCATTCTTGCCACCCTTTCGGGGcc                             >  1:621766/1‑134 (MQ=255)
                                                                                cGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGgcgc                                                                              >  1:950025/1‑83 (MQ=255)
                                                                                cGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGgcgc                                                                              >  2:503570/1‑83 (MQ=255)
                                                                                cGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGgcgc                                                                              <  2:950025/83‑1 (MQ=255)
                                                                                cGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGgcgc                                                                              <  1:503570/83‑1 (MQ=255)
                                                                                cGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGGCATTCTTGCCACCCTTTCGGGGCCGt                           >  1:906623/1‑134 (MQ=255)
                                                                                cGGGCAATGACAATTCGACGGAGGAGACATGAACCAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGGCATTCTTGCCACCCTTTCGGGGCCGt                           >  2:456449/1‑134 (MQ=255)
                                                                                   gCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTc                                                                                        <  2:325712/70‑1 (MQ=255)
                                                                                   gCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTc                                                                                        >  1:325712/1‑70 (MQ=255)
                                                                                               cGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGGCATTCTTGCCACCCTTTCGGGGCCGTCGGCCGATGTCggg             <  1:500515/133‑1 (MQ=255)
                                                                                               cGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGGCATTCTTGCCACCCTTTCGGGGCCGTCGGCCGATGTCggg             <  1:456449/133‑1 (MQ=255)
                                                                                               cGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGGCATTCTTGCCACCCTTTCGGGGCCGTCGGCCGATGTCggg             <  2:948514/133‑1 (MQ=255)
                                                                                                        agaCATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGGCATTCTTGCCACCCTTTCGGGGCCGTCGGCCGATGTCGGGCGCGACCAAt   <  2:514583/134‑1 (MQ=255)
                                                                                                        agaCATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGGCATTCTTGCCACCCTTTCGGGGCCGTCGGCCGATGTCGGGCGCGACCAAt   <  1:545275/134‑1 (MQ=255)
                                                                                                         gaCATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGGCATTCTTGCCACCCTTTCGGGGCCGTCGGCCGATGTCGGGCGCGACCAATa  >  2:281548/1‑134 (MQ=255)
                                                                                                               |                                                                                                                                
ATACCACGGGCGGCGTTCGCCGCCTGACATGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGGCATTCTTGCCACCCTTTCGGGGCCGTCGGCCGATGTCGGGCGCGACCAATA  >  AM260480/507313‑507550

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 

GATK/CNVnator alignment

BRESEQ :: bam2aln output
GCAATACCACGGGCGGCGTTCGCCGCCTGACATGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGGCATTCTTGCCACCCTTTCGGGGCCGTCGGCCGATGTCGGGCGCGACCAATACGATGGCTTTCTGCTTGCCGT  >  AM260480/507310‑507571
                                                                                                                    |                                                                                                                                                  
GCAATACCACGGGCGGCGTTCGCCGCCTGACATGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCG                                                                                                                   >  GWNJ‑0478:712:GW2002102894th:2:2206:13800:72240/1‑150 (MQ=60)
 CAATACCACGGGCGGCGTTCGCCGCCTGACATGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGC                                                                                                                  <  GWNJ‑0478:712:GW2002102894th:2:1208:17277:94525/150‑1 (MQ=60)
                     GCCGCCTGACATGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCC                                                                                              <  GWNJ‑0478:712:GW2002102894th:2:2210:15727:85916/150‑1 (MQ=60)
                               ATGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAG                                                                                    <  GWNJ‑0478:712:GW2002102894th:2:1207:14189:32154/150‑1 (MQ=60)
                               ATGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAG                                                                                    <  GWNJ‑0478:712:GW2002102894th:2:1212:2261:25718/150‑1 (MQ=60)
                                ccgtctcgtgggctcggagatgtgtataagagacagACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAG                                                                                    <  GWNJ‑0478:712:GW2002102894th:2:1216:10105:35339/113‑1 (MQ=60)
                                    GAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAG                                                                               <  GWNJ‑0478:712:GW2002102894th:2:2203:18727:47443/150‑1 (MQ=60)
                                       gggctcggagatgtgtataagagacagCCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGT                                                                             <  GWNJ‑0478:712:GW2002102894th:2:2212:1840:47655/122‑1 (MQ=60)
                                         GTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGctgtctctt                                                                           >  GWNJ‑0478:712:GW2002102894th:2:2212:17434:20140/1‑140 (MQ=60)
                                         GTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGG                                                                          >  GWNJ‑0478:712:GW2002102894th:2:2101:13901:6574/1‑150 (MQ=60)
                                         GTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAacagaaactgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtcttctgcttga                                                                          >  GWNJ‑0478:712:GW2002102894th:2:2210:16656:28711/1‑75 (MQ=60)
                                         GTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAacagaaactgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtcttctgcttga                                                                          >  GWNJ‑0478:712:GW2002102894th:2:2101:20075:37535/1‑75 (MQ=60)
                                                                  CCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCctgtctcttatacacatctccgagcccacgagacggagctacatctcgtatgccgtcttctgctt                                                 >  GWNJ‑0478:712:GW2002102894th:2:2101:7692:88977/1‑85 (MQ=60)
                                                                  CCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGGCATctgtctcttatacacatctccg                                                 >  GWNJ‑0478:712:GW2002102894th:2:1203:4503:85939/1‑128 (MQ=60)
                                                                  CCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGGCATTCTTGCCACCCTTTCGGGGCCG                                                 >  GWNJ‑0478:712:GW2002102894th:2:2108:6778:31936/1‑150 (MQ=60)
                                                                  CCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCCGGCGCgacaagatcgcccgcagccgaacgggattggggctccaggccccatcctgtctcttatacacatc                                                 >  GWNJ‑0478:712:GW2002102894th:2:2213:21078:98435/1‑85 (MQ=60)
                                                                    ACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGctgtctcttatacacatctccgagcccacgagacgg                                                >  GWNJ‑0478:712:GW2002102894th:2:2212:11789:64284/1‑113 (MQ=60)
                                                                    ACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGGCATTCTTGCCACCCTTTCGGGGCCGTC                                               >  GWNJ‑0478:712:GW2002102894th:2:2211:12349:8119/1‑150 (MQ=60)
                                                                       TAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCctgtctcttatacacatctccgagcccacgagacggagctacatctcgt                                             >  GWNJ‑0478:712:GW2002102894th:2:1204:3076:93222/1‑100 (MQ=60)
                                                                                               ATTCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGGCATTCTTGCCACCCTTTCGGGGCCGTCGGCCGATGTCGGGCGCGACCAATACG                     >  GWNJ‑0478:712:GW2002102894th:2:2204:20602:57708/1‑149 (MQ=60)
                                                                                                 TCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGGCATTCTTGCCACCCTTTCGGGGCCGTCGGCCGATGTCGGGCGCGACCAATACGAT                   <  GWNJ‑0478:712:GW2002102894th:2:1216:20562:17951/149‑1 (MQ=60)
                                                                                                 TCGACGGAGGAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGGCATTCTTGCCACCCTTTCGGGGCCGTCGGCCGATGTCGGGCGCGACCAATACGAT                   <  GWNJ‑0478:712:GW2002102894th:2:1212:3762:73796/149‑1 (MQ=60)
                                                                                                          GAGACATGAAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGGCATTCTTGCCACCCTTTCGGGGCCGTCGGCCGATGTCGGGCGCGACCAATACGATGGCTTTCTGC         <  GWNJ‑0478:712:GW2002102894th:2:2102:15983:18930/150‑1 (MQ=60)
                                                                                                                 gaAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGGCATTCTTGCCACCCTTTCGGGGCCGTCGGCCGATGTCGGGCGCGACCAATACGATGGCTTTCTGCTTGCCGT  <  GWNJ‑0478:712:GW2002102894th:2:1202:3828:4412/148‑1 (MQ=60)
                                                                                                                    |                                                                                                                                                  
GCAATACCACGGGCGGCGTTCGCCGCCTGACATGGGGAGCCGTTCAGGCTCGCGTCCGCACGGGCACCACATAGAGTGCCTGACGGGCAATGACAATTCGACGGAGGAGACATGAACAGAAATGCGTGGCAATCGCCGCCGCGCTGGCGCTGCTCGCGCCGGCGCTGGCCAGTGCTGAAGTGAAGGTCGGCATTCTTGCCACCCTTTCGGGGCCGTCGGCCGATGTCGGGCGCGACCAATACGATGGCTTTCTGCTTGCCGT  >  AM260480/507310‑507571

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: