Sample Resequencing Stats
Note: The mutation counts shown below represent unfiltered mutation sets.
| ALE, Flask, Isolate |
Predicted Mutations |
Mean Coverage |
Total Reads |
Percent Mapped |
Mapped Reads |
Average Read Length |
|
A0 F0 I1 R1
|
146 |
27.9 |
2327082 |
89.7% |
2087392 |
107.8 |
Breseq alignment
BRESEQ :: Evidence
|
| evidence |
seq id |
position |
mutation |
annotation |
gene |
description |
| RA |
AM260479 |
2,111,013 |
+G |
coding (553/1089 nt) |
h16_A1942 → |
Hypothetical protein |
|
| |
seq id |
position |
ref |
new |
freq |
score (cons/poly) |
reads |
annotation |
genes |
product |
| * | AM260479 | 2,111,012 | 1 | . | G | 100.0%
| 61.2
/ NA
| 17 | D184E (GAT→GAG) | h16_A1942 | Hypothetical protein |
| Reads supporting (aligned to +/- strand): ref base . (0/0); new base G (8/9); total (8/9) |
CCAGCATGATTCCTCTTCCACAAGCGCAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGAT‑GACTCGACGA‑GATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGCGCGCCGCGCCCGCCGTGCGTCGCGCCGCGGTGCGCGGCG > AM260479/2110900‑2111131
|
ccAGCATGATTCCTCTTCCACAAGCGCAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATc > 1:990313/1‑134 (MQ=255)
ccAGCATGATTCCTCTTCCACAAGCGCAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATc > 2:705708/1‑134 (MQ=255)
ccAGCATGATTCCTCTTCCACAAGCGCAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATc > 2:393821/1‑134 (MQ=255)
cATGATTCCTCTTCCACAAGCGCAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGAcg < 1:705708/134‑1 (MQ=255)
ccACAAGCGCAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCcgccgcgcc < 2:612236/131‑1 (MQ=255)
cgcAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGcc < 1:393821/134‑1 (MQ=255)
ggCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTc < 2:990313/134‑1 (MQ=255)
tgGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGccctcccc > 1:63975/1‑134 (MQ=255)
ggCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTccgccg < 1:478481/118‑1 (MQ=255)
ggCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTccgccg > 2:478481/1‑118 (MQ=255)
ggCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTc > 1:144639/1‑134 (MQ=255)
ccGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGcgcgccgcg < 1:154752/134‑1 (MQ=255)
ccGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCcgccgcgcc > 2:653646/1‑70 (MQ=255)
ccGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCcgccgcgcc < 1:653646/70‑1 (MQ=255)
ccTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGCGCGCCGCGCCCGCCGTGCGTCGCGCCGCGGTg < 2:10956/134‑1 (MQ=255)
cgacgaACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGCGCGCCGCGCCCGCCGTGCGTCGCGCCGCGGTgcg < 2:144639/132‑1 (MQ=255)
cgaACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGCGCGCCGCGCCCGCCGTGCGTCGCGCCGCGGTGCgcggcg > 2:978637/1‑134 (MQ=255)
|
CCAGCATGATTCCTCTTCCACAAGCGCAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGAT‑GACTCGACGA‑GATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGCGCGCCGCGCCCGCCGTGCGTCGCGCCGCGGTGCGCGGCG > AM260479/2110900‑2111131
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
GATK/CNVnator alignment
BRESEQ :: bam2aln output
TGTGGTGGGACCGATCGCCAGCATGATTCCTCTTCCACAAGCGCAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGAT‑GACTCGACGA‑GATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGCGCGCCGCGCCCGCCGTGCGTCGCGCCGCGGTGCGCGGCGTGGCCCAGGCGGT > AM260479/2110883‑2111144
|
gaagacggcatacgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGAT < GWNJ‑0478:712:GW2002102894th:2:2201:14110:22325/90‑1 (MQ=60)
TGGTGGGACCGATCGCCAGCATGATTCCTCTTCCACAAGCGCAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCG > GWNJ‑0478:712:GW2002102894th:2:2207:7696:23712/1‑150 (MQ=60)
GCATGATTCCTCTTCCACAAGCGCAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGC < GWNJ‑0478:712:GW2002102894th:2:2108:4797:51511/150‑1 (MQ=60)
acgagattagcgagtgtctcgtgggctcggagatgtgtataagagacagTGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCAT < GWNJ‑0478:712:GW2002102894th:2:2104:8718:44532/100‑1 (MQ=60)
GCGCAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGC < GWNJ‑0478:712:GW2002102894th:2:1206:19097:44680/150‑1 (MQ=60)
GGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCT > GWNJ‑0478:712:GW2002102894th:2:1102:5909:99389/1‑150 (MQ=60)
agTCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGT < GWNJ‑0478:712:GW2002102894th:2:1210:13335:97256/148‑1 (MQ=60)
TCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCG > GWNJ‑0478:712:GW2002102894th:2:1109:17944:64851/1‑150 (MQ=60)
GCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGCGCGCCGCGCCCGCCGTGCGTCGC < GWNJ‑0478:712:GW2002102894th:2:1111:17219:19165/150‑1 (MQ=60)
TCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCtgtctcttatacacatctccgagcccacgagacactcgctaatctcgtatgccgtcttctgcttgaaaaaa > GWNJ‑0478:712:GW2002102894th:2:1108:4241:67299/1‑78 (MQ=60)
TCGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGCGCGCCGCGCCCGCCGTGCGTCGCGCgcgacctgtctcttatacacatctcc > GWNJ‑0478:712:GW2002102894th:2:2211:8143:97832/1‑124 (MQ=60)
CGACGAACTGGTGGATGGACTCGACGAGGATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGCGCGCCGCGCCCGCCGTGCGTCGCGCgcgaccgaggggagggcgcggcggagc < GWNJ‑0478:712:GW2002102894th:2:1210:5592:53709/150‑28 (MQ=60)
|
TGTGGTGGGACCGATCGCCAGCATGATTCCTCTTCCACAAGCGCAGTTGATCGGGCGCATCGCCAATGTGGCCGGGCGCATGCTCGCCGACGGCGCCGACGAGTATGAGACCTTCGACGAACTGGTGGAT‑GACTCGACGA‑GATGCGATCGACGCCGCCGCGCCGGTGCTGGCCGGCATGGTGCTCCGCCGCGCCCTCCCCTCGGTCGCGCGCGCCGCGCCCGCCGTGCGTCGCGCCGCGGTGCGCGGCGTGGCCCAGGCGGT > AM260479/2110883‑2111144
|
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 0 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
