breseq  version 0.35.4  revision f352f80f4bc9
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 49,791 G→A intergenic (+160/‑32) kefC → / → folA glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase
RA 1,613,627 T→A V84E (GTG→GAG)  marR → multiple antibiotic resistance transcriptional regulator MarR
MC JC 2,399,465 Δ7 bp coding (650‑656/939 nt) lrhA ← transcriptional regulator LrhA
MC JC 3,631,242 Δ66 bp coding (241‑306/1500 nt) pitA → inorganic phosphate transporter PitA
RA 4,594,167 C→T S64F (TCT→TTT)  bglJ → DNA‑binding transcriptional activator BglJ

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NZ_CP009273 356981 357569 589 4 [0] [1] 2 lacA galactoside O‑acetyltransferase
* * ÷ NZ_CP009273 578974 579035 62 2 [1] [1] 2 BW25113_RS26085/appY tail fiber assembly protein/DNA‑binding transcriptional activator AppY
* * ÷ NZ_CP009273 647544 647617 74 2 [1] [1] 2 citC/dpiB [citrate (pro‑3S)‑lyase] ligase/sensor histidine kinase DpiB
* * ÷ NZ_CP009273 724403 724441 39 3 [1] [0] 3 kdpF/ybfA K(+)‑transporting ATPase subunit F/YbfA family protein
* * ÷ NZ_CP009273 968874 968888 15 2 [1] [0] 2 elyC/cmoM envelope biogenesis factor ElyC/tRNA uridine 5‑oxyacetic acid(34) methyltransferase CmoM
* * ÷ NZ_CP009273 982687 982749 63 3 [1] [1] 3 ompF/asnS porin OmpF/asparagine‑‑tRNA ligase
* * ÷ NZ_CP009273 1321109 1321146 38 2 [1] [1] 3 rluB 23S rRNA pseudouridine(2605) synthase RluB
* * ÷ NZ_CP009273 1363732 1363749 18 5 [0] [1] 2 pspD phage shock protein PspD
* * ÷ NZ_CP009273 1459458 1459502 45 3 [0] [1] 2 [BW25113_RS25580] [BW25113_RS25580]
* * ÷ NZ_CP009273 1720020 1720051 32 2 [1] [1] 2 ydhL DUF1289 domain‑containing protein
* * ÷ NZ_CP009273 1783307 1783314 8 3 [0] [1] 2 aroH 3‑deoxy‑7‑phosphoheptulonate synthase AroH
* * ÷ NZ_CP009273 2027379 2027421 43 2 [1] [1] 2 drpB/ompC cell division protein DrpB/porin OmpC
* * ÷ NZ_CP009273 2222527 2222557 31 2 [1] [0] 2 [yohP] [yohP]
* * ÷ NZ_CP009273 2337769 2337796 28 2 [0] [1] 3 yfaL/nrdA AIDA‑I family autotransporter adhesin YfaL/EhaC/ribonucleoside‑diphosphate reductase subunit alpha
* * ÷ NZ_CP009273 3260533 3260568 36 2 [1] [1] 2 tdcA/tdcR transcriptional regulator TdcA/transcriptional activator TdcR
* * ÷ NZ_CP009273 3354472 3354481 10 2 [1] [1] 3 gltD/gltF glutamate synthase subunit GltD/DUF1120 domain‑containing protein

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NZ_CP009273 19797 =NA (NA)15 (0.700) 11/162 0.5 83.5% intergenic (‑2/+14) BW25113_RS25960/BW25113_RS00100 fimbrial family protein/IS1‑like element IS1A family transposase
?NZ_CP009273 = 2808953 3 (0.140)coding (846/1557 nt) gshA glutamate‑‑cysteine ligase
* ? NZ_CP009273 360104 =NA (NA)48 (2.230) 21/164 0.1 62.3% intergenic (‑35/+251) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
?NZ_CP009273 = 360286 29 (1.350)intergenic (‑217/+69) lacZ/BW25113_RS23320 beta‑galactosidase/relaxase/mobilization nuclease domain‑containing protein
* ? NZ_CP009273 1203246 =0 (0.000)14 (0.810) 12/132 0.3 84.9% coding (290/630 nt) BW25113_RS05990 hypothetical protein
?NZ_CP009273 1205075 = 5 (0.290)coding (40/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 12032610 (0.000)11 (0.630) 9/132 0.5 81.5% coding (305/630 nt) BW25113_RS05990 hypothetical protein
?NZ_CP009273 = 1205058 5 (0.290)coding (57/540 nt) BW25113_RS06005 phage tail protein
* ? NZ_CP009273 = 1391494NA (NA)14 (0.660) 8/162 0.8 100% intergenic (+145/‑128) BW25113_RS06980/ynaJ IS5‑like element IS5 family transposase/DUF2534 family protein
?NZ_CP009273 1972747 = 0 (0.000)intergenic (‑293/‑487) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
* ? NZ_CP009273 = 19727510 (0.000)22 (1.020) 16/164 0.3 100% intergenic (‑297/‑483) flhD/uspC flagellar transcriptional regulator FlhD/universal stress protein UspC
?NZ_CP009273 = 2283592 NA (NA)intergenic (‑68/‑387) BW25113_RS11435/narP IS5‑like element IS5 family transposase/nitrate/nitrite response regulator protein NarP
* ? NZ_CP009273 2808944 =2 (0.090)22 (1.020) 15/164 0.3 91.7% coding (855/1557 nt) gshA glutamate‑‑cysteine ligase
?NZ_CP009273 3576788 = NA (NA)coding (391/417 nt) BW25113_RS17825 YrhA family protein
* ? NZ_CP009273 4532499 =5 (0.230)13 (0.680) 9/146 0.6 57.2% intergenic (+49/‑433) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
?NZ_CP009273 4532813 = 15 (0.780)intergenic (+363/‑119) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
* ? NZ_CP009273 = 45325075 (0.230)6 (0.310) 6/146 1.0 38.1% intergenic (+57/‑425) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA
?NZ_CP009273 = 4532803 15 (0.780)intergenic (+353/‑129) fimE/fimA type 1 fimbria switch DNA invertase FimE/type 1 fimbrial major subunit FimA